accutestress disorder treatment
TRANSCRIPT
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ARE YOU STRUGGLING WITH ACUTE STRESS IN YOUR DAILY LIFE?PSYCHIATRIST IN DUBAI IS OFFERING THE SERVICES FOR THE ACUTE STRESS IN
DUBAI.
Acute stress is a common problem in everyone’s life. If you are suffering with the acute stress immediately Contact Us:
www.dubai-psychiatrist.com
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OUTLINE Introduction
Psychosocial stress
Salivary glands
Production of saliva
Catecholamine
Autonomic nervous system
Salivary and norepinephine
Objective
Materials and Methods
Procedures for Trier social stress test (TSST)
Biochemical analyses
Psychometric measurements
Statistical analyses
Results
Discussion and conclusion
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PSYCHOSOCIAL STRESS
Psychological stress occurs when an individual perceives that
environmental demands tax or exceed his or her adaptive
capacity and it affected in sympathetic nervous system (SNS) activity
When psychosocial stress triggers a stress response, the body releases a group of
stress hormones including cortisol, epinephrine (or adrenalin) and dopamine which
lead to a burst of energy as well as other changes in the body
So many people are worrying with the acute stress in their daily life. If you have such
a problems immediately contact our doctor . www.dubai-psychiatrist.com. Our doctro r
is a indian based doctor in the dubai. He is a professional doctor in dubai. He has lot
of experience having a lot of knowledge on how to treat on acute stress
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PSYCHOSOCIAL STRESS
Psychosocial stress was determined by measuring
catecholamine in blood plasma or using electrophysiological
methods
- Skin conductance
- Heart rate
However, these methods are complicated and invasive methods,
therefore, using non-invasive method was interested to determine
psychosocial stress such as investigated salivary alpha-amylase
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SALIVA GLANDS
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PRODUCTION OF SALIVA
Sympathetic cAMP secreted proteins
Parasympathetic Phospholipase C Ca2+ Secreted
fluid
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SALIVARY ALPHA-AMYLASE
(SAA)
http://chemistry.tutorvista.com/organic-chemistry/glycosidic-linkage.html
sAA (α-1,4-α-D-glucan 4-glucanohydrolase)
It is a digestive enzyme that hydrolyses alpha-1,4 bonds of large
polysaccharides such as starch and glycogen, yielding the smaller by-
products of glucose and maltose.
It consists of two families of isoenzymes
• glycosylated
• no carbohydrate
It accounts for 40 – 50 % of total salivary gland-produced protein
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CATECHOLAMINE
norepinephrine
epinephrine
dopamine
A catecholamine (CA) is a monoamine.
CA are compose of …
• norepinephrine
• epinephrine• dopamine
Source
• adrenal medulla
Effects
• physiological changes (fight-or-flight response).
• increases in heart rate, blood pressure, blood
glucose levels
• a general reaction of the sympathetic nervous
system
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AUTONOMIC NERVOUS SYSTEM
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WHY DO RESEARCH DETERMINE SALIVARY ALPHA-AMYLASE AND NOREPINEPHINE
http://www.hakeem-sy.com/main/node/16265
Norepinephrine from sympathetic neurons binds to both
alpha- and beta-adrenergic receptors on the acinar cell and more sensitivity with β2 receptor .
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Acute response of salivary -amylase and plasma norepinephine to the laboratorystress paradism TSST in n = 12 healthy men and women
Rohleder et al., Ann. N.Y. Acad. Sci. 2004; 1032: 25-263.
In previous study
Trier social stress test (TSST)
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OBJECTIVE To examine the correlation between stress-induced
sAA activity with NE and E .
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MATERIALS AND METHODS
Subject
40
18 use oral
contraceptive
(6 smoke)
22 in luteal phase
26 (9 smoke)
BMI 17-27 kg/m2
Age 18-24 years
Free of chronic stress Free of medication (Ex. Beta-block ,glucocorticoid)
free of chronic disease (Ex. Endocrine, cardiovascular)
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PROCEDURES
1
• At first use salivettes to keep a saliva and keep blood sample to EDTA tube.
2• All subject do the ‘Trier social stress test (TSST)’
3• After TSST a second blood draw and saliva
sample was taken again.
4
• At 20 min and 40 min taken a blood sample and saliva sample again.
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TRIER SOCIAL STRESS TEST
(TSST)
For TSST has divided into 2 room
Room A
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Room B
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BIOCHEMICAL ANALYSES
Use enzyme kinetic method was processed on Genesis RSP8/150 liquid handing system
Dilute sample at 1:625 and use 20 µl of dilute saliva and standard transferred into 96 well microplate
Standard prepared from calibrator solution with concentration 326, 163, 81.5 , 40.7, 20.38 ,10.19 and 5.01 U/L of alpha amylase
1. Salivary alpha- amylase (sAA)
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PROCEDURES
Pipette 80 µl of substrate reagent to each well
Incubate at 37 ºc in water bath 90 s
Measurement at 405 nm
Incubate at 37 ºc again for 5 min
Measurement at 405 nm
water bath
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BIOCHEMICAL ANALYSES
2.Norepinephrine and Epinephrine
Plasma sample was analyze for NE and E by
high-pressure liquid chromatography (HPLC) at the Laboratory for Stress Monitoring
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PSYCHOMETRIC MEASUREMENTS
Perceived stress
Depressive symptoms
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PSYCHOMETRIC MEASUREMENTS Perceived stress
Its was assessed with the 14-item version
Perceived Stress Scale (PSS) . PSS is measure
the level of stress by an individual experience
by ranging from 0 ‘never’ to 4 ‘very often’
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PSYCHOMETRIC MEASUREMENTS
Depressive symptoms
This symptom were assessed with the 20-item
of the Center of Epidemiologic Studies Depression Scale
(CES-D) that is a self-report questionnaire about the
incidence and duration of impairment experienced by individuals depressive affects.
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STATISTICAL ANALYSES Use log 10 test for normal distribution
T-test
x ²-test For the assessment of difference between group
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RESULTS
• Men had a significantly higher BMI than both groups of
women .
Smokers and non-smokers differed with a trend in terms
of sex distribution .
Results showed no association of depression scale
values with stress responses of sAA, norepinephrine, or
epinephrine .
Preliminary data analysis
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PRELIMINARY DATA ANALYSIS
Perceive stress scale overall mean indicating no
elevated perceived stress levels in our sample.
Results showed no association between PSS
values and stress responses of sAA,
norepinephrine, or epinephrine .
Results
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RESULTS
Fig. 1. Salivary alpha-amylase (sAA) in response to the acute stress induction
The sAA concentrations
over the course of the four
measurement points, we found a
significant change in the sAA
release over time during the
stress intervention and highestafter TSST .
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RESULTSAfter TSST the results shown that the epinephrineresponse to acute stress was a weak predictor whereasnorepinephine was higher than epinephine.
Fig. 2. Norepinephrine (NE) and epinephrine (E) in response to the acute stress
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Fig. 3. Correlation of stress-induced increases of salivary alpha-amylase (sAA) and norepinephrine (NE).
RESULTS
When correlate between
salivary alpha-amylase
and norepinephrine
response to stress
we found that p = 0.025
and its significant in psychology .
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Correlation of stress-induced increases of sAA and NE
Result from previous study
The results show
that the increasing
of sAA and NE
concentration are
correlate in linear regression .
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DISCUSSION
The results found significant association between stress-
induced sAA and NE responses, but not between sAA
and E responses.
The active secretion of the adrenal medulla contains
approximately 80 % E and 20 % NE ; but this proportion
is reversed in the sympathetic nerves, which contain
predominantly NE , that why NE responses to stress-induced sAA more than E responses.
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DISCUSSION
In the present study we found a significant association
between sAA and NE and the usefulness of sAA to
function as an indicator for SNS activation.
In addition, we found that stress-induced sAA and E
levels in habitual smokers, in individuals with a lower BMI, and in females using oral contraceptive.
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CONCLUSION
From this study show an association between stress
responses of sAA and plasma norepinephrine . This
relationship is stronger than the association of
norepinephrine and epinephrine responses, thus placing
the predictive power of sAA well within the expectedrange for different SNS markers.
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AUTONOMIC NERVOUS SYSTEM
The autonomic nervous system has two main divisions
Sympathetic
Promotes a "fight or flight" response, corresponds with
arousal and energy generation, and inhibits digestion
Parasympathetic
Promotes a "rest and digest" response, promotes
calming of the nerves return to regular function, and
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