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Laboratory. 12 Nov. 2008

•Running an experiment in a completely contained environment as oppose to 2 Liter plastic

•Determine if the pH change that occurs because of T. pseudonana and P. tricornutum has an effect on other organisms living in the same environment

•Observing the effects of other species of Diatoms and their growth with Ferrous Sulfate

Picture 7: Stock cultures of algae maintained for new trials or incase of experimental mishaps

Picture 10: The spectrophotometer was used to measure growth in T. pseudonana and P. tricornutum. The wavelengths used were 660nm and 750nm respectively.

Future Studies

Conclusion

Discussion

Bibliography

•A One-Sample T-Test revealed that there was no significant difference between the CO2, pH and Spectrophotometer measurement between both Thalassiosira pseudonana and Phaeodactylum tricornutum

•In Thalassiosira pseudonana, although the spectrophotometer found more growth in concentration with Ferrous Sulfate as well as a more acidic pH, which indicate the absorption of CO2.

•In Phaeodactylum tricornutum, all data expressed showed that there was more growth in the control rather than the culture ran with Ferrous Sulfate.

•The results of this experiment differ from the work of Coale and Martin as Ferrous Sulfate has no significant effect on the growth of diatoms.

Picture 8: In Trial 2 of data collection, the vessel in growing plankton was altered to a water dispensing jug in an attempt to increase sterility.

•In Conclusion, the results of this experiment differed from the work of Coale and Martin and their idea in exponentially growing diatoms with Ferrous Sulfate.

Picture 9 and 10: The Autoclave machine served as a means of sterilizing certain substances such as media and glassware