The ACQUITY RI Detector.

ACQUITY RI DetectorLow-dispersion, refractive index detection for UPLC and APC has arrived

Universal detection for your non-UV analytes

Combine the ACQUITY RI Detector

with your ACQUITY System to

improve resolution and

throughput for your isocratic

applications, including:

■■ Advanced Polymer

Chromatography (APC)

■■ Food QC Applications

■■ Pharmaceutical USP Methods

■■ Industrial Process Monitoring

ENABLING FAST ER SEPARAT IONS, WIT HOUT COMP ROMISNG DATA QUALIT Y

The key to UPLC® and APC™ separation quality is minimizing the overall system

dispersion. Historically, refractive index detection has had very high dispersion due to the

higher volumes needed to stabilize the detector response. Therefore, adapting refractive

index detectors to low-dispersion LC instrumentation demands specific attention not only

to volume reduction, but also to maintaining a high degree of temperature stability, all

while ensuring critical performance attributes, such as low baseline noise and a wide-

linear dynamic range, are not compromised.

The ACQUITY® Refractive Index (RI) Detector has addressed these challenging technical

and environmental hurdles by providing accurate and precise detection for your non-UV

absorbing analytes, delivering unrivaled quantification for your UltraPerformance LC®

and Advanced Polymer Chromatography™ applications.

Get faster answers for your quality control applications

Laboratories around the world have deployed UPLC technology to improve data quality

and throughput by reducing analysis run times and increasing chromatographic

resolution, making integration and quantification easier and more reliable. These same

benefits can now be realized for samples that contain analytes that require RI detection

due to the absence of a UV-chromophore.

Analysis of the five common food sugars in various matrices on the ACQUITY UPLC® H-Class System with the ACQUITY RI Detector in under four minutes.

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1- Fructose 2- Glucose 3- Sucrose 4- Maltose 5- Lactose

Mixed food sugar standard

Orange juice

Corn syrup

Low fat milk 5

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INNOVATIV E T ECHNOLOGY FOR T HE CHROMATOGRAPHIC LABORATORY

Minimized dispersion enhancements■■ Flow cell volume of 1.3 µL to preserve narrow

UPLC peak volumes

■■ Maintains excellent peak shape

■■ High energy throughput for maximum sensitivity

Refined thermal management■■ Countercurrent heat-exchanger balances the

temperature of in-coming solvent to reduce

baseline drift

■■ Optics housed in a thermally isolated compartment

to minimize environmental fluctuations

Integrated fluidic management■■ Integrated solvent recycle valve empowers you to

reduce costly solvent consumption and is method

programmable

■■ Automatic reference cell purging to maintain

optimal baseline performance

Enhanced control and diagnostics■■ Equipped with a customizable instrument console

giving you access to control, set-up, and status

monitoring features

■■ Simple navigational approach

■■ Diagnostic features at your fingertips

Enhanced data acquisition■■ Sampling rates from 1 to 80 points/s to ensure

accurate definition of your narrow UPLC peaks

■■ Independent optimization of filter time constants

and sampling rates delivers the best combination

of sensitivity and resolution for your application

Enhanced sensitivity■■ High energy LED delivers exceptional energy

transmittance through the flow cell

■■ Long-lifetime lamp with consistent performance

The ACQUITY RI Detector interactive display within the Instrument Console.

Flow cell

End capheat exchanger

Cross tee

Counter currentheat exchanger

Sample inlet flow

Sample outlet flow

SR

Sample flow enters the counter-current

heat exchanger travelling through an

internal capillary into the flow cell. When exiting the flow cell, it flows through a larger I.D. tubing that surrounds the inlet

capillary. This thermally equilibrates the

incoming sample-flow temperature, and

dramatically minimizes drift.

A K EY COMPONENT FOR ADVANC ED POLYMER CHROMATOGRAPHY… LOW-DISPERSION DET ECT ION

The ACQUITY Advanced Polymer Chromatography (APC) System defines a new category of chromatographic polymer analysis that gives you

more information about your polymers, faster than ever before. Combining low-dispersion system fluidics with rigid, small particle columns

of a wide range of pore sizes, the system defines the ultimate in polymer peak resolution, particularly for low molecular weight oligomers.

One of the key enablers of this break-through technology is low-dispersion, refractive index detection. GPC applications are typically limited

to larger I.D. columns due to the impact of the detector’s dispersion on the resolution of the polymeric peak. The effect of system dispersion

can result in incorrect molecular weight averages and polydispersity measurements. However, the low dispersion in the ACQUITY RI

Detector makes it entirely compatible with narrow, high resolution APC columns, which promotes reduced solvent consumption and provides

unprecedented resolution of low molecular weight polymers.

The same 103 to 105 polystyrene standards were run on an ACQUITY APC System with a standard HPLC refractive index detector [A], and the ACQUITY RI Detector [B].

The loss in resolution due to the HPLC detector volume is clearly evident when zoomed in to the 103 standard. When the HPLC detector is used, all of the resolution of the low MW oligomers is lost to the HPLC detector’s dispersion.

The high-resolution separation achieved with the ACQUITY RI Detector on an ACQUITY APC System provides better characterization of low MW polymers and their oligomers, such as this epoxy resin.

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Epoxy resinACQUITY RI detectionEluent: THFACQUITY APC XT 4.6 x 150 mm x 2 (125 Å/45 Å)

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Epoxy resinACQUITY RI detectionEluent: THFACQUITY APC XT 4.6 x 150 mm x 2 (125 Å/45 Å)

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

SIMPLIF IED MET HOD T RANSFER - TAK E YOUR CURRENT HPLC / RI MET HODS TO T HE NEX T LEV EL

The benefits of transferring your existing HPLC methods to UPLC have been

demonstrated time and time again, across multiple application types. Now, HPLC

methods that require refractive index detection can also be seamlessly transferred

to UPLC. Intuitive tools, including the ACQUITY UPLC Columns Calculator and

the Reversed-Phase Column Selectivity Chart, are available to help you select the

proper UPLC stationary phase and scale your existing HPLC/RI methods to your

UPLC/RI system.

Benefits of UPLC Analysis:

■■ Improved chromatographic resolution

■■ Increased sample throughput

■■ Reduced solvent consumption and cost per analysis

Waters, ACQUITY UPLC, UPLC, UltraPerformance LC, and Waters Quality Parts are registered trademarks of Waters Corporation. Advanced Polymer Chromatography, APC, and T he Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2013 Waters Corporation. Printed in the U.S.A.March 2013 720004636EN TC-PC

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The ACQUITY UPLC H-Class System with the ACQUITY RI Detector.

Topi

ram

ate

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HPLC

UPLC

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Topi

ram

ate

The transfer of the HPLC USP method for Topiramate to the ACQUITY UPLC H-Class System with the ACQUITY RI Detector has resulted in an 80% reduction in runtime while maintaining equivalent chromatographic performance.