acquity uplc column compartments operator’s overview and … · 2012-01-17 · overview 1...

ACQUITY UPLCColumn Compartments Operator’s Overview

and Maintenance Information

Revision C

Copyright © Waters Corporation 20102011All rights reserved

ii

Copyright notice

© 20102011 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

Considerations specific to the column compartments

Warning: Do not use the column heater with solvents that have auto ignition temperatures below 150 C.

Warning: When dealing with hazardous solvents, run the system in a hooded environment that provides a sufficient air exchange rate.

Warning: To prevent injury, always observe Good Laboratory Practices when you handle solvents, change tubing, or operate the column heater. Consult the Material Safety Data Sheets and the Solvent Considerations section of the ACQUITY UPLC System Guide regarding the solvents you use.

Table of Contents

Copyright notice ................................................................................................... ii

Considerations specific to the column compartments ................................. ii

Overview ................................................................................................................ 1 Column compartment types ................................................................................ 1

Preparing the column compartments ........................................................... 14 Installation recommendations for fittings........................................................ 14 Installing the standard column heater............................................................. 17 Installing the 30-cm column heater.................................................................. 39 Installing the column manager......................................................................... 45 Installing one CM-Aux module, in a single-stack configuration..................... 48 Installing two CM-Aux modules in a multiple-stack configuration................ 54 Installing the ACQUITY 30-cm column heater/cooler..................................... 82 Installing the optional column in-line filter ..................................................... 87 Installing the column......................................................................................... 89

Using the column compartments ................................................................... 98 Control panels .................................................................................................... 98 Resolving column compartment leak sensor errors ......................................... 99

Maintaining the column compartments ..................................................... 110 Contacting Waters technical service............................................................... 110 Maintenance schedule ..................................................................................... 112 Maintenance considerations............................................................................ 113 Configuring maintenance warnings ............................................................... 113 Replacing the column heater’s leak sensor .................................................... 114 Replacing the 30-cm column heater’s leak sensor ......................................... 119 Replacing the column manager’s leak sensor................................................. 119 Replacing the column manager switching-valve cartridge............................ 122 Replacing the column ...................................................................................... 125 Replacing the active preheater assembly ....................................................... 127 Replacing the column stabilizer assembly (CH-A only)................................. 133 Replacing the frit in the in-line filter unit...................................................... 136

Table of Contents iii

Replacing the column heater/cooler fuse (ACQUITY UPLC 30-cm CHC).... 139 Cleaning the instrument’s exterior (all models) ............................................ 140

iv Table of Contents

Overview

Column temperature variations can shift peak retention times and alter peak shapes, increasing the difficulty of achieving precise results. The ACQUITY UPLC® column compartments help to ensure precise, reproducible separations by controlling the column temperature.

Note: If you are using an ACQUITY UPLC system with two dimensional applications, refer to the ACQUITY UPLC Systems with 2D Technology Capabilities Guide.

Column compartment types

The following column compartments are available for use with ACQUITY UPLC systems:

• Column heater (CH-A)

• 30-cm column heater (CH-30A)

• Column manager (CM-A)

• Auxiliary column manager (CM-Aux)

• 30-cm column heater/cooler (ACQUITY UPLC 30-cm CHC)

Each of these column compartments is also available in a bio-compatible version.

Column heater (CH-A)

A standard component of the basic ACQUITY UPLC system, the column heater heats the column compartment to any temperature, from 20ºC to 90 ºC, that is at least 5 ºC above the ambient temperature. A standard, active preheater heats the incoming solvent before it enters the column. Additionally, you can install a supplementary column stabilizer.

The column heater can accommodate UPLC® columns with dimensions of as much as 4.6 mm, internal diameter (ID), and 150-mm long.

To reduce dispersion associated with dead volume, and minimize the length of tubing between system instruments, the column compartment is directly above the sample manager and connected to the optical detector (on top of the column heater). For MS applications, you can mount the column compartment, on a bracket to the right-hand side of the system stack, reducing the distance to the mass spectrometer’s inlet probe.

Overview 1

A receptacle on the column heater’s right-hand side receives the column's eCord™ chip. The eCord column chip stores column manufacturing and usage data, which you can access from the ACQUITY UPLC Console.

The column heater does not have its own power supply; it draws power from the sample manager. Thus the column heater is automatically powered-on when you power on the sample manager.

Restriction: The ACQUITY column heater (CH-A) cannot be used in conjunction with the optional 30-cm column heater (CH-30A).

Active preheater

The standard, active preheater uses an electric heating element to directly heat the column inlet tubing. The rapid heating, low volume design reduces gradient delay and extra-column bandspreading. It ensures that high flow rate separations occur at the column temperature, not at the temperature of the incoming solvent. By controlling the temperature of the mobile phase at the point of use—the column inlet—temperature differences introduced by variations in the system configuration are eliminated.

Active preheater:

If you use the active preheater when running an HPLC or UPLC method originally developed for use with a column stabilizer, adjusting the column temperature can be necessary.

2

The following diagram shows the location of the column heater in the ACQUITY UPLC system.

TP03240

Bottle tray

Detector

Column heater (CH-A)

Sample manager - flow through needle / Sample manager - fixed loop

Solvent manager

Overview 3

Column heater, major components:

The following diagram shows the column heater’s major components.

Column heater components:

Component Description

Column compartment

Contains the column, column heater, in-line filter, and active preheater or column stabilizer assembly. The column compartment insulates the column from changes in ambient temperature. For MS applications, the assembly can be mounted on an external bracket on the right-hand side of the chassis, reducing the distance to the inlet probe of a mass spectrometer.

In-line filter Protects the analytical column against contamination by trapping impurities before they reach the column.

Active preheater assembly

Heats the incoming solvent to the specified column temperature before the solvent enters the column.

Active preheater key

In the 30-cm column heater, installed in the control port not occupied by the active preheater.

Active preheater retainer

Secures the active preheater in the column compartment.

eCord receptacle Receives the column eCord™ chip, which stores column manufacturing and usage data.

Column compartment tray

Location of column

Fitting Active preheater retainer


4

30-cm Column heater (CH-30A)

The optional 30-cm column heater heats the column compartment to any temperature, from 20ºC to 90 ºC, that is at least 5 ºC above ambient. The 30-cm column heater mounts on the side of the system stack.

An active preheater heats the incoming solvent before it enters the column. The active preheater can be located at the top or bottom of the column compartment, to accommodate UPLC or HPLC columns. You can disable the active preheater when solvent pre-heating is unnecessary.

Column stabilizer (optional)

Passively heats the incoming solvent before the solvent enters the column.

Drip tray Captures any leakage from the column or column connections and routes it into the drip management system.

Column heater components: (Continued)


Overview 5

The column heater can accommodate UPLC® columns of 4.6 mm, or less, ID and 150 mm, or less, length and HPLC columns of 4.6 mm, or less, ID and 300 mm, or less, length. For columns that exceed 4.6 mm ID, use the ACQUITY UPLC 30-cm column heater/cooler with the appropriate compatibility kit.

A receptacle atop the column heater receives the column’s eCord™ chip. The chip stores column manufacturing and usage data, which you can access from the ACQUITY UPLC Console.

The column heater does not have its own power supply; it draws power from the sample manager. Thus the column heater is automatically powered-on when you power on the sample manager. Do not use the 30-cm column heater (CH-30A) in conjunction with the standard ACQUITY column heater (CH-A).

6

30-cm column heater components:

Column manager (CM-A) and auxiliary column manager (CM-Aux)

The column manager can regulate the temperature of as many as four columns between 4 and 90 °C. The temperature of each column trough can be independently set in 0.1 °C increments. The column manager can be used for column switching applications as well as two dimensional applications. For two dimensional applications, see the ACQUITY UPLC Systems with 2D Technology Capabilities Guide.


Location of active preheater or active preheater key

Location of active preheater or active preheater key

Location of column

Drip tray with leak sensor

Overview 7

Note: The minimum achievable column compartment temperature set point cannot be greater than 25 °C below ambient temperature.

A supplementary pre-heating device (active preheater) can also be installed, to heat the incoming solvent before it enters the column.

The column manager also offers a bypass channel and automated, programmable switching between columns for methods development. ACQUITY UPLC™ columns are equipped with eCord Information Management Technology, which captures the history of each column to assist in tracking column usage. Reusable high-pressure fittings ease replacement of the columns, when needed.

The column manager can accommodate columns with inside diameters of up to 4.6 mm. It can accommodate two 100- or 150-mm columns or four 50-mm columns.

Receptacles on the column manager’s front panel receive the column eCord™ chips. The eCord column chip stores column manufacturing and usage data, which you can access from the ACQUITY UPLC Console.

Column manager major components

The following diagram shows the column manager’s major components.


eCord receptacles

Active preheater retainer Location of active

preheater assembly

Drip tray with leak sensor

Column switching-valves

8

Auxiliary column manager

In addition to the column manager, the system can include one or two auxiliary column manager modules. The column manager acts as a master to the optional auxiliary column manager modules. Although the auxiliary column manager modules have their own power supply, the column manager controls their power state.

Auxiliary column manager modules are not fitted with switching valves. All plumbing connections are made to the switching valves in the column manager.

The auxiliary column manager can accommodate two 100- or 150-mm columns with inside diameters of up to 4.6 mm.

Each auxiliary column manager module consists of two independently heated and cooled column troughs. Like the column manager, the temperature range of the auxiliary column manager module is 4 to 90 °C. Each trough can accommodate one column of 50 to 150-mm, and an active preheater.

Column manager and auxiliary column manager components:


Column compartment

Contains the column, column heater, in-line filter, and active preheater assemblies. The column compartment insulates the column from changes in ambient temperature.

In-line filter Protects the analytical column against contamination by trapping impurities before they reach the column.


Heats the incoming solvent to the specified column temperature prior to entry to the column.

Active preheater retainer

Secures the active preheater in the column compartment.

Column switching-valves (CM-A only)

The inlet and outlet column switching-valves allow for programmable switching access to any column installed in the column manager as well as to column bypass mode and to waste.

Drip tray Captures any leakage from the column or column connections and routes it into the drip management system.

Overview 9

Auxiliary column manager:

Column manager configurations

You can configure the column manager for use with two columns (in any combination of 50-, 100-, or 150-mm columns), or with as many as four, 50-mm columns. To accommodate additional 100- or 150-mm columns, the ACQUITY UPLC system can include one or two auxiliary column manager (CM-Aux) modules (a maximum of six columns per system).

These configurations are available for an ACQUITY UPLC system including a CM-Aux:

• Column manager configured for two columns and, in addition, one auxiliary column manager (as many as four, 100- or 150-mm columns, total)

• Column manager configured for two columns and, in addition, two auxiliary column managers as many (as six, 100- or 150-mm columns, total)

To reduce dead volume, and ensure optimal performance, you must plumb all column manager configurations using preconfigured tubing lengths. Therefore, the modules must occupy specific positions in the system stack. One auxiliary column manager can rest atop the column manager, and the column manager can rest atop a second auxiliary column manager.

Note: See the appropriate system guide for supported system configurations.

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Examples of column manager configurations:

Single stack

Dual stack

Triple stack

TUV

Sample

Solvent

CM-Aux

CM-A

TUV

SQD

CM-A

CM-Aux

PDA

CM-Aux

CM-Aux

CM-A

PDA

CM-Aux

CM-A

ELS

TUV

SQD

CM-A

ELS

CM-Aux

CM-Aux

CM-Aux

CM-APDA

ELS

PDA

CM-Aux

CM-Aux

CM-A

SQD

ELS

CM-Aux

PDA

CM-Aux

CM-Aux

CM-A

ELS

SQD

PDA

CM-Aux

CM-A

Manager

Manager

SolventManager

SolventManager

SolventManager

SolventManager

SolventManager

SolventManager

SolventManager

SolventManager

Sample Manager

Sample Manager

Sample Manager

Sample Manager

Sample Manager

Sample Manager

Sample Manager

Sample Manager

Overview 11

For each column in a two- or four-column configuration, you can use an active preheater. In the two-column configuration, the column manager supports guard columns or filters. When the column manager is configured for four 50-mm columns, you cannot use guard columns or filters (see page 10).

ACQUITY UPLC 30-cm column heater/cooler (ACQUITY UPLC 30-cm CHC)

The ACQUITY 30-cm column heater/cooler is a combination forced-air-convection heater and cooler with a temperature range of 20 to 65 °C.

30-cm column heater/cooler:

The ACQUITY UPLC 30-cm column heater/cooler offers these features for ease of use:

• Adjustable column clips that slide, for easy column mounting.

• Extended column compartment that allows for the use of two 300-mm columns, with column guards; four 150-mm columns, without column guards; or six 50-mm columns, without column guards.

• Grommet in the column mounting plate, for attaching a calibrated probe (thermistor) for validation.

12

• Single-piece column compartment door and hinge assembly that allows tubing to enter or exit the column compartment from all four sides.

• Columns with dimensions of up to 7.8 mm diameter.

When you specify the temperature via the operating software, thermoelectric devices achieve the desired column temperature. The column heater/cooler’s internal power supply provides the additional power required to operate the thermoelectric heater/cooler engine circuit.

Door gasket

Compartment door

Tubing guide

Drip tray

Compartment door clasp

Column clip rails

Temperature port

Column clip

Convection grid

Overview 13

Preparing the column compartments

Installation recommendations for fittings

The system uses gold-plated compression screws and two-piece ferrules. See the diagram below for assembly orientation.

Recommendations:

• To prevent bandspreading, ensure the tubing bottoms in its fitting hole before tightening the compression screw.

• For easier accessibility, use long compression screws to attach tubes to the injector and vent valve.

• Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads, and deformations.

• Do not reuse stainless steel fittings more than six times.

• When installing or removing a column or optional in-line filter, always hold the active preheater’s reusable compression fitting in place and rotate the column or in-line filter onto the fitting.

Warning: To avoid the harmful effects of personal contact with fittings contaminated with biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when reinstalling the fittings.

TubingCompression screwFerrule with locking ring

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When tightening system fittings, consult the following table.

Installation recommendations for ACQUITY UPLC column compartment fittings:

Fitting Recommended tightening

Fir

st u

se

Reusable finger-tight Finger-tight, plus 1/6-turn

Rei

nst

alle

d

Reusable finger-tight Finger-tight, plus up to 1/6-turn; if leaking, tighten another 1/8-turn

Fir

st u

se

Stainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule

Finger-tight, plus 3/4-turn using wrench

TP02728

1/6-turn

Collet removal tool

1/6-turn

Additional 1/8-turn

TP03378

Long flats 2-piece ferrule

3/4-turn

Preparing the column compartments 15

Rei

nst

alle

dStainless steel (gold-plated) fitting with long flats and 2-piece stainless steel ferrule

Finger-tight, plus up to 1/6-turn using wrench

Fir

st u

se o

r re

inst

alle

d

Dual-threaded finger-tight fitting with stainless steel cap nut

1. Loosen the stainless steel cap nut from the gold fitting.

2. Finger tighten the gold fitting, with the ferrule, into the column.Requirement: If you are reinstalling the fitting, replace the PEEKTM ferrule.

3. Rotate the column, and tighten the column onto the gold fitting.

4. If you are installing the column in a system with pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), tighten the column an additional 1/2-turn.

5. Tighten the stainless steel cap nut onto the gold fitting.

Installation recommendations for ACQUITY UPLC column compartment fittings: (Continued)

Fitting Recommended tightening

TP02713

Long flats 2-piece ferrule

1/6-turn

TP03360A Gold fitting

Ferrule

Stainless steel cap

16

Installing the standard column heater

If your system includes the standard column heater, follow the procedures below to install it.

Installing the optional MS extension bracket

If your system includes a mass spectrometer, use the optional MS-bracket to position the column heater to the right of the ACQUITY UPLC system stack. Doing so reduces the length of the tubing that extends to the mass spectrometer inlet.

Consult the ACQUITY Configuration Guide to determine whether your system requires the MS-bracket kit.

Installing the MS-bracket kit entails these actions:

• Removing the column heater from the device chassis

• Installing the MS-bracket on the column heater chassis

• Attaching the column heater housing onto the MS-bracket

• Adjusting the height of the column heater bracket, if necessary

• Attaching the column heater and connecting accessories, such as the MS drip tray, leak sensor, and drain tubing

• Connecting inlet and outlet tubing

Follow the instructions below to install the MS-bracket kit.

Required materials

• Column heater MS-bracket kit

– MS-bracket

– Column heater housing

– MS drip tray

– Height adjustment bracket

– Hinge assembly

– Cable clamp

– TORX® screws


– Phillips® screws

– Cable clamp nut

• Drain tubing

• Gloves: clean, powder-free, chemical-resistant

Required tools

• 1/4-inch open-end wrench

• 4-mm open-end wrench

• 3-mm hex wrench

• Phillips screwdriver

• Small flat screwdriver

• T20 TORX driver

MS-bracket kit major components:

TP03252

MS-bracket

Column heater housing

MS drip tray

Height adjustment bracket

Hinge

18

To install the MS-bracket:

1. Stop any currently running mobile phase.

2. Power-off the sample manager.

3. Using the 1/4-inch open-end wrench, disconnect the column inlet, active preheater, or column stabilizer tubing from injector port 6 of the sample manager.

4. Disconnect the column-outlet PEEK® tubing from the detector inlet.

5. Open the column heater door, and remove the column.

Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment internal temperature to ensure all components are cool.

Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when preparing the column heater for use with a mass spectrometer.

Column, active preheater, or column-stabilizer-inlet tubing


6. Remove the column stabilizer assembly, if fitted.

a. Using the Phillips screwdriver, loosen the two screws that fasten the column stabilizer assembly to the column compartment tray.

b. Remove the column stabilizer assembly from the thermal gasket.

c. Remove the thermal gasket from the column tray.

7. Remove the active preheater assembly, if fitted.

a. Remove the active preheater assembly from the retainer in the column compartment.

TP03425

Location of Phillips screws

Thermal gasket


20

b. Using a small screwdriver, gently pry out the active preheater retainer, and remove it from the column compartment.

8. Remove any solvent lines routed behind the column heater door.

9. Detach the leak sensor connector from the front of the sample manager, if fitted.

10. Remove the TORX screw from the right-hand side of the column heater, just behind the eCord receptacle.

Gently compress retainer here

TORX screw

eCord receptacle


11. Gently pull the right-hand side of the column heater toward you, and then slide it to the right, to unseat the tab on the left-hand side of the column heater from the device’s chassis.

12. Remove the 25-pin cable from the receptacle on the back of the column heater.

13. Set the column heater aside.

14. Ensure that the front left-hand foot of the column heater’s rear enclosure locks into the retainer bracket on the sample manager below it.

Alternative: If the retainer bracket is not installed on the sample manager chassis, perform these tasks:

a. Remove the solvent tray and any system modules stacked atop the column heater’s rear enclosure.

Warning: To avoid injuries arising from solvent spills, remove the solvent bottles from the solvent tray before removing the solvent tray.

Caution: To avoid spilling solvent when disassembling the system, remove the solvent bottles first. Then remove the solvent tray, and finally the modules, one at a time. Ask an assistant to help lift the components off the system stack.

1

2

22

b. Remove the Phillips screw that secures the column heater’s rear enclosure to the sample manager chassis.

c. Remove the column heater’s rear enclosure from the sample manager chassis.

d. Secure the retainer bracket, supplied in the column heater startup kit, onto the sample manager chassis using the TORX screw provided in the kit.

e. Install the column heater’s rear enclosure sliding its front, left-hand foot into the retainer bracket on the sample manager’s chassis.

Retainer bracket


f. Install the Phillips screw that secures the column heater’s rear enclosure to the sample manager chassis.

g. Replace any other instruments and the solvent tray above the column heater’s rear enclosure.

15. Install the MS-bracket on the sample manager’s chassis:

a. Insert the tab on the left-hand side of the MS-bracket into the slot on the left-hand side of the sample manager chassis.

Tab

Slot

24

b. Route the 25-pin cable behind the MS-bracket and through the slot on the right-hand side of the bracket.

c. Install the TORX screw in the tab, and tighten it slightly.

d. Hold the left-hand side of the bracket into the slot, and guide the MS-bracket tab on the right-hand side into the cutout in the sample manager’s chassis.

Requirement: Ensure that the 25-pin cable remains in the slot as you guide the tab into the cutout. If the cable slips out of the slot, it can prevent the bracket from seating fully into the chassis.

e. Tighten the TORX screw in the tab.

f. Tighten the 3-mm Allen screw on the left-hand side of the MS-bracket.

Route the 25-pin cable through this slot

Tab

Cutout


16. Attach the column heater housing onto the MS-bracket using the hinge:

a. Attach the short side of the hinge to the MS-bracket using two TORX screws.

b. Attach the long side of the hinge to the column heater housing using two TORX screws.

17. Swing the column heater housing, to align it with the front of the mass spectrometer, and ensure that the column heater is at the correct height for the mass spectrometer inlet.

18. If the column heater housing height is not correct for the mass spectrometer inlet, perform these tasks:

a. Remove the two TORX screws that secure the hinge to the MS-bracket.

MS-bracket

Column heater housing

Remove these TORX screws

26

b. Using two TORX screws, attach the height adjustment bracket to the MS-bracket.

c. Using two TORX screws, attach the hinge assembly on the column heater housing to the height adjustment bracket.

d. Swing the column heater housing, aligning it with the front of the mass spectrometer, and ensure that the column heater is at the correct height for the mass spectrometer inlet.

e. If the column heater housing is not at the correct height, adjust the height by removing the hinge from the height adjustment bracket and then reattaching it using different holes.

19. Attach the MS drip tray to the column heater housing using the Phillips screws.

Tip: Installing the leak sensor is optional.

TORX screws

MS-bracket

Height adjustment bracket

Locations of Phillips screws

Drip tray

Leak sensor cable slot


20. Install the leak sensor:

a. Align the leak sensor’s T-bar with the slot in the left-hand side of the MS-bracket drip tray, and slide the leak sensor into place.

b. Route the leak sensor cable through the slot in the column heater housing.

21. Secure the 25-pin cable:

a. Insert the 25-pin cable into the cable clamp.

b. Install the cable clamp on the threaded stud inside the column heater housing, and then thread the 4-mm nut onto the stud.

TP02892

Leak sensor T-bar

Slot

28

c. Tighten the cable clamp nut with the 4-mm wrench.

22. Install the column heater in its housing:

a. Insert the tab on the left-hand side of the column heater into the slot on the left-hand side of the housing.

b. Hold the left-hand side of the column heater into the slot, and guide the eCord tab, on the right-hand side, into the cutout in the column heater housing.

c. Install the TORX screw in the tab and tighten it.

25-pin cable routed through slot

4-mm cable clamp nut on stud

Cable clamp

Tab

Cutout


23. Push the 25-pin cable into the socket on the back of the column heater, and then tighten the thumbscrews to secure the cable to the socket.

24. If you installed the optional leak sensor, plug the connector into the receptacle located behind the column heater.

25. Swing the column heater into the operating position.

26. Push the drain tubing onto the barbed drain fitting on the left-hand side of the MS-bracket drip tray, and route it to a suitable waste container.

27. Open the column heater door.

Thumbscrews

Location of leak sensor receptacle

30

28. Slide the column compartment tray to the right.

29. If desired, install the active preheater assembly:

a. Gently compress the active preheater assembly retainer between your thumb and forefinger, and push it into the left-hand side of the column compartment until it snaps into place.

b. Install the active preheater assembly in the retainer.

30. Install the column in the column tray:

a. Snap the column support clips onto the body of the column (one between the reusable fitting and column). Rotate the clips so that the openings face forward.

b. Attach the mass spectrometer inlet tubing included with the system to the column outlet.

c. Set the column into the column compartment.

Requirement: Ensure that the stainless steel tubing from the sample manager is routed through the front notch on the lower, left-hand side of the column compartment and that the PEEK


Column support clip


tubing to the mass spectrometer inlet is routed through the groove on the right-hand side of the column compartment.

d. If you installed the active preheater, connect the column inlet to the active preheater by holding the active preheater’s reusable compression fitting in place, rotating the column onto the fitting until it is snug, and then tightening an additional 1/6 turn.

e. Connect the column inlet or active preheater tubing to injector port 6 of the sample manager.

Tip: To ensure that the sample manager door closes properly, and does not damage the tubing, route the tubing as shown in the drawing below.

f. Attach the column-outlet tubing to the mass spectrometer’s inlet.

Tip: Consult the sample manager’s documentation for more information about connecting to the inlet.

Column inlet or active preheater inlet tubing

32

g. Attach the eCord fob to the receptacle on the side of the column heater.

h. Close the column heater door.

Installing the optional column stabilizer assembly

The column heater’s optional column stabilizer reduces sensitivity to ambient temperature swings by transferring heat (via conduction) from the column heater’s film element through the thermal gasket to the column inlet tubing. The looped tubing path reduces dispersion while ensuring maximum surface area contact between the tubing and the column tray, maximizing heat transfer to the mobile phase.

The passive column stabilizer provides thermal equilibration by heating the incoming mobile phase and injected sample to the specified column temperature before they enter the column.

Note: You cannot use the active preheater and the passive column stabilizer simultaneously; choose the preheater type that best suits your application.

Receptacle

eCord fob


Typical column stabilizer:

The column stabilizer’s passive design lends itself to lower flow rate and higher volume applications. If you are running an HPLC or UPLC method that was originally developed for use with a column stabilizer, you must continue to use the column stabilizer when you run that method on the ACQUITY UPLC system.

Requirement: You must remove the active preheater assembly before installing the column stabilizer assembly.

For instructions on how to replace the column stabilizer assembly, see page 133.


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when installing the optional column stabilizer assembly.

34

Required materials


• 50/100-mm or 150-mm column stabilizer kit:

– Collet and compression screw multi-tool (collet separator)

– Column stabilizer assembly

– PEEK tubing for connecting the column to the detector’s flow cell

– 3 Phillips screws (1 is a spare)

– 3 thermal gaskets (2 are spares)

Required tools

• Needle-nose pliers


To install the optional column stabilizer assembly:

1. Stop any currently running mobile phase before replacing the column stabilizer assembly.

2. Disconnect the inlet tubing from injector port 6 of the sample manager.


Location of column- stabilizer-inlet tubing


4. Remove the active preheater assembly from the retainer in the column compartment.

5. Using a needle-nose pliers, gently compress the top and bottom of the active preheater retainer, and pull it out of the column compartment.

6. Remove the clear, protective backing from the thermal gasket, and with the thermal gasket oriented vertically, center it between the two threaded holes.


Gently compress retainer here

Location of thermal gasket

Threaded holes

36

7. Press the thermal gasket into the column compartment tray, ensuring the gasket is centered and formed into the bottom of the tray, with equal lengths on both sides.

8. Firmly press the column stabilizer assembly into the thermal gasket, ensuring the thermal gasket uniformly envelops the sides of the column stabilizer and does not bunch beneath it.

Column stabilizer assembly (two styles):

TP03425

Column-stabilizer-inlet tubing

Threaded holes for Phillips screws

Thermal gasket visible along both sides of stabilizer

TP03123

Reusable fittings

Inlet tubing to injector

For 150-mm column For 50-mm or 100-mm column


9. Carefully route the column-stabilizer-inlet tubing through the groove on the side of the tray.

10. Insert the Phillips screws into the threaded holes, and tighten them gently using the Phillips screwdriver.

11. Route the column-stabilizer-inlet tubing through the cutout in the top of the sample manager door to the injector.

Requirement: Do not route the tubing behind the sample manager door hinge.

12. Remove the O-ring retainer from the inlet end of the column-stabilizer inlet-tubing, and then connect the inlet tubing to injector port 6 of the sample manager injector.

Caution: To avoid stripping the threads, do not overtighten screws.


Note: Do not route the tubing between the sample manager door and hinge, here.

38

Installing the 30-cm column heater

If your system includes a 30-cm column heater, follow the procedure below to install it.

Required materials


• Screws: M4 × 50-mm, stainless steel (2), startup kit

• Side mount shaft (2), startup kit

• Side mount collar (2), startup kit

Required tools

• 1/4-inch bit driver

• 2.5-mm hex driver

• 7-mm adjustable wrench

• T20 TORX screwdriver

To install the 30-cm column heater:

1. Assemble the side-mount collars onto the side-mount shafts, and position the collars so that they are located at the base of the shafts' threads.


Caution: To avoid damaging the electronic components of column heaters fitted with a rear DSUB cable, unplug the cable from the sample manager before performing this procedure.

Caution: To avoid damaging electrical components, do not disconnect an electrical assembly from an instrument or device that remains connected to a building’s electrical supply. To completely interrupt power, set the unit’s power switch to Off. Then disconnect the power cord from the unit’s rear panel or from the wall outlet. After discontinuing power, wait 10 seconds before attempting to disconnect an assembly.


3. Using the T20 TORX screwdriver, remove the top screw from the right-hand side of the sample manager; remove the bottom screw from the right-hand side of the solvent manager.

Right-hand side of system stack:

Remove screws

Detector

Sample manager

Solvent manager

40

4. Insert the two M4 × 50-mm long screws into the side-mount shafts, from the side with the knurled, side-mount collars.

5. Screw the side-mount shafts into the top, right-hand side of the sample manager and the bottom, right-hand side of the solvent manager.

Insert M4 × 50-mm long screws here

Install side-mount shafts here

Detector

Sample manager

Solvent manager


6. Mount the CH-30A by sliding the large key-hole slots onto the side mount shafts.

7. Turn the knurled, side-mount collars counter-clockwise, to pinch the CH-30A sheet metal chassis within the mount for a secure fit.

8. Connect one end of the pin cable to the socket on the 30-cm column heater’s rear panel.

Key-hole slots

Knurled side mount collar

42

9. Connect the other end of the pin cable to the socket on the sample manager’s rear panel.

10. Connect the drain tubing to the barbed fitting on the bottom front of the column heater.

11. Route the other end of the drain tubing to a suitable waste container.

Sample manager - FTN

30-cm column heater

Connect the pin cable between these sockets

Drain fitting


Moving the preheater key

When the CH-30A is shipped, the preheater key is installed in the upper, active preheater control port, thus readying the active preheater for use with a 30-cm column heater and 300-mm (30-cm) column. If you intend to use the active preheater with shorter tubing and a shorter column, you must move the preheater key to the lower control port.

Requirements:

• Always install the preheater key in the control port not already occupied by the active preheater.

• Do not insert two active preheater assemblies in the CH-30A simultaneously. The preheater key must always occupy one of the active preheater control ports for the instrument to function.

The following procedure describes how to move the preheater key.

Required materials


• Preheater key assembly (startup kit)

Required tools

Needle-nose pliers

To move the preheater key:

1. Remove the active preheater assembly from the retainer in the column compartment.

2. Using a needle-nose pliers, gently compress the top and bottom of the active preheater retainer, and withdraw it from the column compartment.

44

3. Gently press the preheater key assembly into the upper or lower-control port in the column compartment.

4. Using a needle-nose pliers, install the active preheater retainer, in the control port where the active preheater will be installed.

5. Insert the active preheater intended for use.

Installing the column manager

If your system includes a column manager, follow the procedure below to install it.

Plumbing the column manager

Ensure you have the correct tubing kit for your column compartment configuration (column manager and optional auxiliary column manager modules).

Connect the column manager tubing, as shown in the diagram below.

When making plumbing connections, follow the guidelines on page 14.

Warning: To avoid back injuries, two or more people must lift the column manager.

Warning: To avoid crushing your fingers beneath or between modules, use extreme care when installing the column manager in the system stack.

Warning: To avoid overheating the column manager, do not block the air vent on its side.

Insert this end into control port


Two-column configuration, with inlet switching-valve on the right-hand side:

Note: See the appropriate site preparation guide for information regarding which tubing and valve kit to use.

Two-column configuration plumbing connection details:

From ToLength (inches)

Part number

H-Class I-Class

CM-A inlet valve, port B

CM-A outlet valve, port B

N/A 430002725, bypass tubing, .005-inch ID)

430002725, bypass tubing, .004-inch ID)

CM-A inlet valve, port Wa

Solvent manager front drip tray waste port

N/A 430002727, waste tubing, .010-inch ID

430002727, waste tubing, .004-inch ID

3

4

12

B

3

45

WI

12

B

3

45

6

O

6

WPinPlug

6

Injector

Column manager

Outlet switching valve

Inlet switching valve

To detector

Sample manager

Column position 1

Column position 2

46

CM-A inlet valve, port 3

Column 1 inlet 12.5 205000730, APH

205000894, APH


Column 2 inlet 12.5 205000730, APH

205000894, APH

Column 1 outlet CM-A outlet valve, port 3

19 430002683, SST tubing, .004-inch ID (reorder part number 700005478)

430003151, SST tubing, .003-inch ID (reorder part number 700008868)

Column 2 outlet CM-A outlet valve, port 4

19 430002683, SST tubing, .004-inch ID (reorder part number 700005478)


Sample manager injector valve, port 6

CM-A inlet valve, center port

14.5 430002582, SST tubing, .004-inch ID (reorder part number 700005476)


Detector inlet CM-A outlet valve, center port

14.5 430002681, SST tubing, .004-inch ID (reorder part number 700005477)


a. Be sure to connect the 430002727 waste tubing to port W (at the 3 o’clock position) of the inlet valve. Do not connect to the center port.

Two-column configuration plumbing connection details: (Continued)


Part number

H-Class I-Class


Notes:

• The two-column configuration for the ACQUITY UPLC I-Class system uses low-volume tubing with high-pressure fittings.

• When installing the active preheater on the column inlet, use a wrench to tighten the gold fitting onto the column an additional 1/4-turn.

• The dual-threaded finger-tight fitting secures the tubing to the column outlet in ACQUITY UPLC I-Class systems.

Installing one CM-Aux module, in a single-stack configuration

The single-stack configuration supports up to four columns (in any combination of 50-, 100- or 150-mm columns). The inlet valve is located on the right-hand side of the column manager.

Required materials


• 4-column tubing set, for installing the upper auxiliary column manager (CM-Aux)

Required tools



• 2-mm Hex wrench



• T10 TORX driver

• T20 TORX driver

• Right-angle nut extender tool, for low-pressure fittings

• Stainless steel tubing cutter, for cutting waste tubing to length

Repositioning instruments in the system stack

To reposition the instruments in the system stack:

1. Power-off the column manager (CM-A) and detector.

48

2. Disconnect the power and Ethernet cables from the rear panel of the CM-A and detector.

3. Remove all solvent bottles from the bottle tray.

4. Remove the solvent tray from the system stack.

5. Disconnect the detector’s waste tubing and the inlet tubing from the CM-A outlet.

6. Remove the detector from the system stack.

7. Place the CM-Aux on the CM-A.

8. Place the detector on the CM-Aux.

9. Place the solvent tray on the detector.

Warning: To avoid injuries arising from personal contact with spilled solvent, remove the solvent bottles from the solvent tray before removing the solvent tray.


10. Place the solvent bottles in the solvent tray.

Front view of repositioned instruments:

11. Connect the power cables to the CM-Aux, CM-A, and detector.

12. Connect the external control cable between the CM-A’s upper auxiliary connector and the CM-Aux.

Detector

CM-Aux

CM-A

Sample manager

Solvent manager

Bottle tray

50

13. Connect the Ethernet cables to the rear panel of the CM-A and detector.

Rear view of repositioned instruments:

Setting up waste management

To set up the waste management components:

1. Route a waste line from the barbed fitting on the rear of the solvent tray to a suitable waste container.

2. Route the waste tube from the detector to the solvent manager drip tray (using the backpressure regulator) or to another in-line detector.

Detector

CM-Aux

CM-A

Sample manager

Solvent manager

Bottle tray

External control cable


3. Install the 4.125-inch, convoluted drip tube (part number 430002826) from the drip tray of the CM-Aux to the front waste port of the CM-A’s drip block.

Tip: To avoid leaks, ensure that the convoluted drip tube is fully inserted into the front waste port of the CM-A’s drip block.

CM-Aux

Convoluted drip tube

CM-A drip block

52

Plumbing the CM-A and CM-Aux

Tip: To avoid damaging the tubing and column compartment cover latches, ensure the tubing is routed through the grooves in the sides, top, and bottom of the column compartment.

Location of grooves in sides, top, and bottom of column compartment:

To plumb and configure the CM-A and CM-Aux, consult these pages:

• For tubing connection and fittings information, see page 68, page 76, and page 77.

• For information about configuring the new column configuration in the software, see page 78.

• For column installation information, see page 81.

Grooves(3) on right side and top of column compartment

Location of grooves (3) on left side and bottom of column compartment


Installing two CM-Aux modules in a multiple-stack configuration

These configurations can support either four or six columns (in any combination of 50-, 100-, or 150-mm columns). The inlet valve is located on the left-hand side of the column manager.

Required materials


• 4-column tubing set, for installing the upper auxiliary column manager (CM-Aux)

• 6-column tubing set, for installing the lower CM-Aux

Note: When installing the lower auxiliary column manager in a multiple stack configuration, you need the following parts from the startup kit:

– Extended feet and four screws

– Swivel drain kit and Tygon® tubing

Required tools



• 2-mm Hex wrench



• Right-angle nut extender tool, for low-pressure fittings

• Stainless steel tubing cutter, for cutting waste tubing to length

• T8 TORX driver

• T10 TORX driver

• T20 TORX driver

To establish a multiple-stack configuration, follow these general steps, in order:

1. Remove all fittings from the CM-A valves.

2. Select a new system module configuration.

3. Install extended feet, as needed (see page 57).

54

4. Reposition the instruments for the new configuration.

5. Set up waste management, as needed.

6. Exchange the valve cartridges.

7. Install new magnetic diagrams (see page 68).

8. Install the tubing.

9. Configure all columns in the software.

Removing the fittings from the inlet and outlet valves

To remove the fittings from the inlet and outlet valves:





5. Push the latches atop the CM-A’s column compartment cover inward, to disengage them from the latch pins.

Warning: To avoid injuries arising from personal contact with spilled solvent, remove the solvent bottles from the solvent tray before removing the solvent tray.


6. Tilt the column compartment cover forward, just enough to clear the valve drip tray.

7. Pull the column compartment cover up and away from the CM-A.

8. For each fitting installed on the inlet and outlet valves of the CM-A, mark the fitting with the number of the port to which it is connected.

9. Disconnect all fittings from the inlet and outlet valves.

10. Remove the columns and the active preheaters from the column troughs.

Selecting a system module configuration

To select a system module configuration:

1. Identify each instrument module in your system.

2. Referring to the diagram on page 11, select a system module configuration that includes your new column compartment(s).

Latches

Latch pins

Valve drip tray

Column compartment cover

56

Installing the extended feet on the bottommost column module

The extended feet allow room for installation of the swivel drain. Verify that the extended feet are installed on the bottommost column module, the CM-A or the CM-Aux, in the system module configuration you choose.

To install the extended feet on a CM-A or a CM-Aux:

1. Turn the CM-A or CM-Aux so that it is resting on it’s side.

2. Using the T20 TORX driver, remove the screws that secure the four feet to the bottom of the CM-A or CM-Aux.

3. Using the T20 TORX driver and M4 25-mm screws, fasten the short and long rubber feet onto the bottom of the CM-A or CM-Aux.

4. Place the CM-A or CM-Aux (now the “lower” CM-Aux) on the bench, to the right of the solvent manager, ensuring at least 3 inches space remains between the modules, for ventilation.

Screws (4)

Short rubber foot (4)

Long rubber foot (4)

T20 TORX driver

M4 25-mm screw (4)


Repositioning instruments in the system stack

Requirement: When repositioning the instruments, ensure the bottommost column module, the CM-A or the CM-Aux, is fitted with the drip tray equipped with a leak detector (see page 59).

To reposition the instruments in the system stack:





5. Disconnect the waste tubing from the detector and the inlet tubing from the CM-A outlet.

6. Remove the detector from the system stack.

7. Remove the CM-A waste tubing from the solvent manager drip tray.

8. Remove the sample manager outlet tubing from port 6 of the injector.

9. Arrange the instrument modules in the new configuration.

10. Place the solvent tray in the appropriate location in the system stack.

11. Place the solvent bottles in the solvent tray.

12. Connect the power cables to the upper CM-Aux, CM-A, lower CM-Aux (if present), and detector.

13. Connect the external control cables to the rear of the CM-A as follows:

• Upper auxiliary port to upper CM-Aux

• Lower auxiliary port to lower CM-Aux (if present)

14. Connect the Ethernet cables at the rear panels of the CM-A and detector.

Warning: To avoid injuries arising from contact with spilled solvent, remove the solvent bottles from the solvent tray before removing the solvent tray.

58

Setting up waste management

To set up the waste management for a lower CM-Aux:

1. Route the detector waste tube to the solvent manager drip tray.

2. Route the outlet of the detector flow cell to waste (using the backpressure regulator) or to another in-line detector.

3. Using the T20 TORX driver, loosen the upper and middle screws that secure the CM-A drip block to the module.

Upper screw

Middle screw


4. Using the T20 TORX driver, remove the lower screw, which secures the CM-A’s drip tray to the module.

5. Remove the drip block assembly from the CM-A, separate the drip tray from the drip block, and then set the drip tray and block aside.

6. Repeat step 3 through step 5, to remove and separate the drip tray and drip block for the lower CM-Aux module.

Lower screw

Drip block

Drip Tray

60

7. Insert the CM-Aux drip tray into the CM-A drip block, and then insert the CM-A drip tray into the CM-Aux drip block.

8. Reinstall the CM-A drip block assembly (drip block and CM-Aux drip tray) onto the CM-A module, and then secure it in place using the three T20 TORX screws.

Requirement: Tighten the upper and middle screws, and replace the drip tray screw.

9. Install the 4.125-inch convoluted drip tube (part number 430002826) from the upper CM-Aux drip tray to the front waste port of the CM-A drip block.

CM-Aux drip tray

CM-A drip block

CM-A drip tray

CM-Aux drip block


Tip: To avoid leaks, ensure that the convoluted drip tube is fully inserted into the front, waste port of the CM-A drip block.

10. Install the 2.0-inch convoluted drip tube (part number 430002827) onto the CM-A drip tray.

Upper CM-Aux

Convoluted drip tube (4.125-inch)

CM-A drip block

Convoluted drip tube (2.0-inch)

CM-A drip tray

CM-A

Lower CM-Aux

62

11. Insert the open end of the convoluted tube into the front waste port of the lower CM-Aux’s drip block, and carefully reinstall the drip block assembly onto the lower CM-A, as shown in the figure below.

Tips:

• Use your left hand to support the drip tray.

• You can loosen or tighten the two, T20 TORX, securing screws slightly, so that the screw heads can fit into the slots in the drip block.

• If the internal drip tube extends into the drip block, use needle-nose pliers to extend the tube outward approximately 0.25 inches.

CM-A drip tray

Lower CM-Aux drip block

Extending the tube approximately 0.25 inches


Installing the swivel drain and waste tubing

To install the swivel drain and waste tubing:

1. Push the latches atop the CM-A’s column compartment cover inward, to disengage them from the latch pins.

2. Tilt the column compartment cover forward, just enough to clear the valve drip tray.

3. Pull the column compartment cover up and away from the CM-A.

4. Remove the mounting screw and washer from the swivel drain cup.

Latches

Latch pins

Valve drip tray


Washer

Mounting screw

64

5. Install the swivel drain cup on the bottom of the drip tray, and thread the mounting screw through the hole in the top of the drip tray.

6. Use a T8 TORX driver to minimally tighten the mounting screw.

Requirement: Do not overtighten the screw.

Caution: To avoid leaks, ensure the O-ring is properly seated in the swivel drain cup.

Swivel drain cup

CM-A drip tray

Mounting screw and washer

Hole in top of drip tray

O-ring


Tips:

• If you cannot swivel the drain cup, the mounting screw is too tight. Loosen the mounting screw until the drain cup swivels freely.

• In a multiple-stack configuration, the swivel drain is installed in the bottommost column module.

7. Slide the Tygon waste tubing over the drain fitting and route the tubing to a suitable waste container.

Exchange the CM-A inlet and outlet switching-valve cartridges

In a multiple stack configuration, you can place the CM-A module to the right of the primary stack. In such a configuration, you must locate the inlet switching-valve, on the left-hand side of the module.

Tip: Exchanging the switching-valve cartridges is optional. Nevertheless, doing so ensures that all the fittings originally installed on a valve are reinstalled on the same valve in the new configuration. If you elect not to exchange the switching-valve cartridges, proceed to “Installing the magnetic diagram” on page 68.

To exchange the CM-A inlet and outlet switching-valve cartridges:

1. In the ACQUITY UPLC Console software, select Column Manager from the system tree.

2. In the column manager information window, click Interactive Display.

3. In the Column Manager Interactive Display dialog box, click Control.

Drain fitting

66

4. Ensure the inlet switching-valve is set to Waste.

Tip: To change the setting to Waste, click System and select Waste.

Switching-valve cartridge:

5. Use the 2-mm hex wrench to remove the hex screw at the 10 o’clock position on the switching-valve cartridge.

6. Remove the switching-valve cartridge from the switching-valve assembly by pulling it straight outward.

7. Label the inlet switching-valve cartridge.

8. Repeat step 1 through step 7 for the outlet switching-valve cartridge.

9. For both inlet- and outlet switching-valve cartridges, ensure that the groove in the cartridge housing aligns with the groove on the drive clamp.

Requirement: If the grooves fail to align, turn the drive clamp until they do so, taking care to avoid scratching the drive clamp or housing.

2-mm hex screwLock ring stop

Lock ring

Stator ID

Switching-valve cartridge housing

Drive clamp

Aligned grooves


10. Insert the inlet switching-valve cartridge into the switching-valve cartridge chamber on the left-hand side of the column manager.

Requirement: Ensure the new cartridge assumes the same orientation as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters® Technical Service.

11. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench.

12. Insert the outlet switching-valve cartridge into the switching-valve cartridge chamber on the right-hand side of the column manager.

Requirement: Ensure the new cartridge assumes the same orientation as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service.

13. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm hex wrench.

Installing the magnetic diagram

To install the magnetic diagram:

Install the appropriate column compartment diagrams, provided in the tubing kit, in the front doors of the upper and lower CM-Aux modules.

Installing the tubing

To install the tubing:

1. Refer to the following figures and tables for plumbing details on your configuration.

2. Install the waste (port W located at the 3 o’clock position on the valve) and bypass (port B) tubing before making any other tubing connections.

3. For valve connections, pull the column manager’s switching-valve forward, 1/2 to 3/4 inches, and rotate the lock ring clockwise until it rests against the stopper.

4. Use the 1/4-inch wrench to reattach existing valve fittings and tighten them 1/6-turn beyond finger-tight. For new fittings see page 76.

68

Tip: Use a pen to coil any excess tubing on any of the connections.

5. When all valve connections are made, rotate the valve’s lock ring counterclockwise, to the 12 o’clock position.

6. Push the switching-valve assembly inward until it stops.

7. Plug all unused ports on the inlet and outlet switching valves using pin plugs.

Four-column configuration, with inlet switching-valve on the right-hand side:

TubingRouter

3

4

12

B

3

45

WI

12

B

3

45

6

O

6

W

6

To detector

Column position 1

Column position 2

APH

APH

Column Manager-Aux

APH

APH

Column position 3

Column position 4

Drip tray

Column Manager

Sample Manager



Drip tube

Injector

Drip tray with leak detector


Four-column configuration, with inlet switching-valve on the left-hand side:

3

4

12

B

3

45

WO

12

B

3

45

6

I

6

W

TubingRouter

TubingRouter

To detector

APH

APH

Column Manager-Aux

APH

APH

Column Manager

Column position 1

Column position 2

Column position 3

Column position 4

From inject valve

Drip tray

Drip tube


LC detector

Extended feet



70

Six-column configuration, with inlet switching-valve on the left-hand side:

3

4

12

B

3

45

WO

12

B

3

45

6

I

6

W

TubingRouter

TubingRouter

To detector

APH

APH

Column Manager-Aux

APH

APH

Column Manager

Column position 1

Column position 2

Column position 3

Column position 4

From inject valve

To pump waste

APH

APH

Column position 5

Column position 6

Column Manager-Aux

Drip tray

Drip tube

Drip tray

Drip tube


Extended feet




Four-column configuration in a single CM-Aux (50-mm columns), with inlet switching-valve on the right-hand side:

Four and six column configuration plumbing connection details:


Part number

Stainless steelMP35N(H-Class Bio)

CM-A inlet valve, port B

CM-A outlet valve, port B

N/A 430002725 (bypass tubing, .005-inch ID)

430002779

CM-A inlet valve, port Wa

Solvent manager front drip tray waste port

N/A 430002727(waste tubing, .010-inch ID)

430002727


Column 1 inlet

12.5 279002002, APH (reorder part number 205000730)

279002003 (reorder part number 205000756)

APH

APH



APH

APH


Sample manager

Column manager

Column position 1

Column position 3

Column position 2

Column position 4

Injector valve

72


Column 2 inlet




Column 3 inlet




Column 4 inlet




Column 5 inlet




Column 6 inlet



Column 1 outlet

CM-A outlet valve, port 1

19 430002683, tubing, .004-inch ID (reorder part number 700005478)


Column 2 outlet




Four and six column configuration plumbing connection details: (Continued)


Part number



Column 3 outlet




Column 4 outlet




Column 5 outlet


22.5 430002657, tubing, .004-inch ID (reorder part number 700005480)


Column 6 outlet





CM-A inlet valve, center port





Part number


74


CM-A inlet valve, center port (I)

19b 430002599, tube assembly, .004-inch ID ×19.0-inch long (reorder part number 700005479)


CM-A outlet valve, center port

Detector inlet 14.5 430002681, tubing, .004-inch ID (reorder part number 700005477)



Detector inlet 19 430002683, tubing, .004-inch ID (reorder part number 700005478)



Detector inlet 22.5b 430002657, tubing, .004-inch ID (reorder part number 700005480)


a. Be sure to connect the 430002727 waste tubing to port W (at the 3 o’clock position) of the inlet valve. Do not connect to the center port.

b. Depending on your configuration, it may be necessary to order extra lengths of this tubing.



Part number



Identifying switching valves and active preheaters

The following table lists the features that identify standard and bio system switching valves and active preheaters.

Note: Tubing contained in the SST or MP35N column tubing kits does not have an identification sleeve.

Assembling the new fittings

For fitting assemblies with ferrules not previously assembled or set to tubing, you must mark the compression screw and fitting body, and ensure the two marks line up when you tighten them.

To assemble the new fittings:

1. Insert the end of a tube into the hexagonal end of the compression screw.

2. Insert the tube into the larger end of the ferrule.

3. Insert the tube into the fitting body.

4. Rotate the compression screw, clockwise, into the fitting body until the screw is finger-tight.

5. Using a permanent marker, mark the compression screw at the 12 o’clock position.

6. Mark the fitting body at the 9 o’clock position.

7. Ensure the tubing makes contact with the bottom of the fitting body, and use the 1/4-inch open-end wrench to rotate the compression screw clockwise 3/4-turn until the two marks line up.

Switching valve and active preheater identification features:

ComponentH-Class system identification features

H-Class Bio system identification features

I-Class system identification features

9-position switching valve

Stainless steel valve cartridge, stator ID = 11

Titanium valve cartridge, stator ID = 12

Stainless steel valve cartridge, stator ID = 22

Active preheater

White sleeve, labeled 316SST

Blue sleeve, labeled MP35N

White sleeve, labeled .003

76

Installing dual-threaded fittings

To install the dual-threaded fittings:

1. Loosen the stainless steel cap nut from the dual-threaded, finger-tight fitting.

APH dual-threaded fitting with stainless steel cap nut:

2. Ensure the stainless steel tubing protrudes beyond the PEEK ferrule.

3. Apply the axial load towards the column hardware and insert the stainless-steel tubing with the ferrule into the detail of the column hardware.

4. Seat the ferrule by finger tightening the gold fitting into place.

Requirement: Do not rotate the stainless-steel silver cap nut when seating the ferrule.

Tip: When making a new connection to a replacement column, replace the PEEK ferrule.

5. While holding the gold fitting, tighten the connection by rotating the column hardware back onto the gold fitting.

6. If you are installing the column in a system with pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), tighten the column an additional 1/2-turn.

7. Complete the connection by finger tightening the stainless-steel silver cap nut onto the fitting.

TP03360A

Gold fitting

Ferrule

Stainless steel tubing

Stainless steel cap nut


Configuring the instruments

To configure the instruments:

1. Power-on the CM-A and detector.

2. From the ACQUITY UPLC Console system tree, select TUV detector > Control > Reset TUV.

3. From the ACQUITY UPLC Console system tree, select Column Manager > Configure > Instrument.

Four-column configuration:

78

Six-column configuration:

Note: When using Empower 1154 or Empower 2 on a system containing CM-A and CM-Aux modules, you cannot select positions 5 and 6 from the column selection field of the sample queue. Instead, create an instrument method for a specific column position (5 or 6, for example) and save it with a unique name. From within the sample queue, select the appropriate method set (that included the instrument method you created) to specify the column position.


Four-column configuration using 50 mm columns:

4. Enable the upper and lower CM-Aux, as necessary, and then click OK.

Tip: When you enable the upper CM-Aux, the default CM-A column numbers change to 3 and 4.

5. In the Configuration Confirmation window, click OK, reboot the CM-A, and then click OK to restart the ACQUITY UPLC Console.

6. From the ACQUITY UPLC Console, click Configure > Arrange Instrument Modules, and then click and drag the instrument modules to appropriate locations, if necessary.

Advanced mode

When using the advanced mode of operation, you can directly control two internal, 6-port, 2-position valves in the CM-A through the Advanced Mode features in the inlet method editor.

80

Notes:

• Advanced Mode features are not compatible with older column managers (CM).

• You can use as many as three of the following external valves with the CM-A:

Monitoring the CM-A and CM-Aux LEDs

Light emitting diodes on the CM-A and each CM-Aux indicate the instrument’s state of functioning.

Power LED

The CM-A’s power LED, on the left-hand side of the front panel, is steady green to indicate that the instrument is powered on. The CM-Aux’s power LED, also on the left-hand side of the front panel, is steady green after the CM-Aux is configured (in the ACQUITY UPLC Console) and the CM-A is rebooted. The power LED is unlit after the CM-Aux is removed from the instrument configuration and the CM-A is rebooted.

Run LED

The run LED, on the right-hand side of the power LED on the CM-A’s and CM-Aux’s front panel, is steady green when the instrument with the program-selected column (either the CM-A or CM-Aux) is running a method. The run LED is unlit when the instrument is idle and functioning normally.

Installing the columns

To install the columns:

1. Refer to the ACQUITY UPLC Console, locate column position 1, and install the column in position 1. When installing the column, be sure to install the column clip.

CM-A external valves:

External valve Part number

10-port, 2-position, Titanium 417000138

7-port, 6-position, PEEK 417000139

11-port, 10-position, stainless steel 417000150


2. Connect the column inlet and outlet of the column in position 1.

3. Connect the eCord to the port on the right side of the upper CM-Aux.

4. Run flow through the column with backpressure to ensure that there are no leaks.

5. Install the columns in the other positions as desired and test for leaks.

Installing the ACQUITY 30-cm column heater/cooler

If your system includes an ACQUITY 30-cm column heater/cooler, follow the procedure below to install it.

Required materials

• Bracket kit


Required tools

• 1/4-inch bit driver

• 7-mm adjustable wrench

• T20 TORX screwdriver

To install the ACQUITY 30-cm column heater/cooler:


Caution: If your system includes a column heater with a rear DSUB cable, to avoid damaging the device’s electronics, unplug the cable from the sample manager before performing this procedure.


82

2. Using the T20 TORX screwdriver, remove the top screw from the right-hand side of the sample manager, and the bottom screw from the right-hand side of the solvent manager.

Right-hand side of system stack:

Remove screws

Detector

Sample manager - FTN

Solvent manager


3. Mount the rear tab of the adaptor bracket onto the ground stud at the rear of the sample manager.

Bracket

Ground stud

84

4. Insert two M4 × 16 screws into the slots on the bracket’s left-hand panel, leaving the first screw loose until both screws are inserted. Then tighten both screws.

5. Add an M4 Keps® nut to the ground stud at the rear of the sample manager, and tighten with a 7-mm adjustable wrench.

M4 Keps nut:

M4 × 16 screws

Ground stud

M4 side panel spacers with cover screws


6. Insert the three, M4, blue, cover screws through the three side-panel spacers, and then screw them into the three threaded holes in the bracket’s center panel.

7. Mount the column heater/cooler onto the bracket, fitting the column heater/cooler mounting holes over the M4 cover screws.

8. Using a 1/4-inch bit driver, attach the black, tubing clips to the front of the bracket.

TP02939

Spacer Cover screw

Mounting holes

86

9. Load the clip into the bit driver in the orientation desired, and then snap into the bracket.

Installing the optional column in-line filter

You can install a 0.2-m in-line filter to protect the analytical column and other system components from a build-up of particulate matter, improving column lifetime and minimizing system downtime.


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when installing the optional column in-line filter.

TP02945

Tubing clip

1/4-inch bit driver


Required materials

• Column in-line filter


• Reusable fitting

Required tools

5/16-inch open-end wrench

For instruction on how to replace the filter in the column heater’s in-line filter unit, see page 136.

To install the column in-line filter:

1. Remove the O-ring retainer from the outlet end of the active preheater or column stabilizer tubing.

Tip: Before connecting, make sure that the ferrule is approximately 3 mm from the end of the outlet tubing and that the collet is touching the back of the ferrule.

Older, reusable fittings do not have holes in their sides.

Caution: To prevent contaminating system components, wear powder-free, non-latex gloves when connecting tubing and tightening fittings.

TP02846 Reusable fitting

Ferrule

3 mmCollet

Outlet tubing

88

2. Remove the column in-line filter unit from its packaging, and then push the outlet end of the active preheater or column stabilizer tubing assembly into the in-line filter inlet until it stops.

Installing the in-line filter:

3. Holding the reusable fitting in place, rotate the in-line filter unit onto the fitting until it is snug.

4. Finger tighten the fitting until it is snug, and then tighten it up to an additional 1/6-turn.

Installing the column

The procedure for installing the column varies depending on the column heater model.

Caution: To avoid damaging the tubing, use only your fingers to tighten the tubing fittings.


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when installing the column.

TP02873

Flow

In-line filter unitColumn From column stabilizer tubing assembly

Inlet

Reusable fitting


Installing the column (CH-A, CM-A, CM-Aux, and CH-30A)

Note: Waters ships the CH-30A with a tubing kit that includes several column inlet and exit tube options. ACQUITY instrument stack configurations can vary, so you must configure the shortest possible column exit tube to the detector. Whenever possible, use reusable fittings, like the one-piece plastic ones included in the start-up kit. When you use a guard column and a 300-mm column on the same system, you must minimize path length. To do so, use the metal fitting sets with the short, gold-coated compression screws, such as those found on the 60-cm tube. You can also use short fittings with the active preheater-to-column tube. Otherwise, use the active preheater-to-column tube with reusable fittings. The kit also includes a 120-cm length of PEEK plastic tubing, for custom configurations.

Required materials

• Column support clips


• High-temperature outlet fitting

• High-temperature outlet fitting wrench

• Reusable fitting

Required tool

5/16-inch open-end wrench

For instruction on how to replace the column in the column heater, see page 125.

To connect the column in-line filter unit and the column inlet:

1. Remove the O-ring from the outlet end of the column in-line filter unit.

2. Remove the plug from the column inlet.

Caution: To avoid damaging the active preheater assembly,• turn the column, not the fitting, when installing or removing the

column to prevent the active preheater tubing from turning with the fitting.

• do not grasp and turn the assembly.• do not suspend or hang the active preheater tubing.

90

3. Push the outlet end of the column in-line filter unit into the column inlet until it stops.

4. Holding the finger-tight reusable fitting in place, rotate the column onto the fitting until it is snug, and then tighten it up to an additional 1/6-turn.

To install the PEEK tubing in the column outlet:

1. Insert the PEEK tubing into the high-temperature outlet fitting until it stops.

2. Holding the PEEK tubing, thread the high-temperature outlet fitting into the column outlet until it is finger-tight.

3. Using a 5/16-inch wrench to hold the column steady, use the high-temperature outlet fitting wrench to give the outlet fitting 1/4-turn.

4. Carefully route the PEEK tubing through the column compartment.

Caution: To avoid damaging the tubing, use only your fingers to tighten the fittings.

Caution: To avoid bandspreading, ensure that the tubing is fully bottomed in the fitting hole before tightening it.

Caution: To avoid damaging the outlet fitting, do not overtighten it with the outlet fitting wrench.


To connect the PEEK tubing to the detector:

1. Remove the protective cover from the PEEK tubing from the flow cell inlet in the detector.

2. Ensure the label on the flow cell inlet tubing matches the type of detector and flow cell in your system.

3. Attach the 0.004-inch or .0025-inch ID inlet tubing (included with the flow cell or the stabilizer kit) to the column outlet.

4. Snap the column support clips onto the body of the column (one between the reusable fitting and column, and rotate the clips so that their openings face forward.

5. Place the column in the column compartment.

TP02952Detector inlet tubing (PEEK) from column

Outlet

Inlet

Column support clip

92

6. Attach the eCord fob to the receptacle:

• Column heater – the receptacle is located on the right-hand side.

• 30-cm column heater – the receptacle is located on the top of the device’s chassis.

Heater compartment receptacle

eCord fob

Receptacle


• Column manager – the receptacle is located inside the front panel. Consult the figure below to determine the correct placement.

• Auxiliary column manager – the receptacle is located on the right-hand side of the device’s chassis. Consult the figure below to determine the correct placement.

7. Close the column compartment:

• Column heater and 30-cm column heater – close the door, and then inspect the seal around the front panel, to ensure it aligns correctly.

Note: Proper alignment of the seal is critical for a stable thermal environment.

• Column manager and auxiliary column manager – align the tabs in the column compartment cover with the slots, push the cover closed, and then push the latches outward until they snap onto the latch pins. When the column compartment cover is in place, close the door.

eCord fob

2 column configuration: Bottom column4 column configuration: Bottom right column

2 column configuration: Top column4 column configuration: Top right column

4 column configuration:Bottom left column

4 column configuration:Top left column

Top column trough

Bottom column trough

94

Tip: To avoid inconsistent results due to temperature fluctuations, ensure the column heater's or column manager's door latch is completely closed.

Column compartment door in closed position:

Installing the columns (ACQUITY UPLC 30-cm CHC)

You can install multiple chromatographic columns within the compartment of the column heater or column heater/cooler. The table below shows the possible column configurations. Columns are not provided with the column heater/cooler.

Warning: To avoid electric shock, power-off the sample manager before you connect or disconnect the external communication cable (between the sample manager and the column heater).

Caution:

• To avoid damage to the components, power-off the sample manager before you connect or disconnect the external communication cable (between the sample manager and the column heater).

• To avoid damaging electrical components, do not disconnect an electrical assembly from an instrument or device that remains connected to a building’s electrical supply. To completely interrupt power, set the unit’s power switch to Off. Then disconnect the power cord from the unit’s rear panel or from the wall outlet. After discontinuing power, wait 10 seconds before attempting to disconnect an assembly.


Secure columns within the heater/cooler compartment using adjustable column clamps attached to the column rail. You can add multiple clamps to the rail that are held in place when the column is inserted. The column clamps are designed to hold onto the compression screw threads, to accommodate the columns of various manufacturers. They do not damage the screw threads.

ACQUITY UPLC 30-cm CHC column configurations:

Quantity Description

1 300-mm length column: 7.8-mm ID, or less, with a column guard of 20-mm, or less

2 300-mm length column: 7.8-mm ID, or less, with column guards of 20-mm, or less

4 150-mm length column: 7.8-mm ID, or less, without column guards

6 50-mm length column: 7.8-mm ID, or less, without column guards

Column guard

Column

Column clamp

Column rail

Column clamp

96

Tubing retainers provided along the left-hand side, bottom, and top of the column compartment hold tubing in place. Use these retainers to organize tubing and keep it orderly.

Tubing retainers:

The heater or heater/cooler column compartment functions only when you can properly close the door. The door’s gasket and hinge assembly allow tubing to pass through undamaged.

To activate auxiliary column manager units:

1. Click the Enabled check box corresponding to the auxiliary column manager units you want to activate.

Restriction: When the icon of an auxiliary column manager is unavailable for selection, the software does not detect the device.

2. Click OK.

Caution: To avoid causing the compartment door’s gasket to fail or the door to spontaneously spring open, do not route tubing out the bottom of the door.


Using the column compartments

Control panels

Column manager control panel

Where Empower software controls the system, the column manager’s control panel appears at the bottom of the Run Samples window. Where MassLynx software controls the system, the column manager's control panel appears on the Additional Status tab of the Inlet Editor window.

Column manager control panel:

The column manager control panel displays the current column temperature and set point. You can edit the set point when the system is idle by clicking on

Warning: To avoid explosion, do not use the column heater with solvents whose autoignition temperatures are below 150 C.

Warning: To avoid breathing harmful fumes or vapors, when using hazardous solvents, run the system in a hooded environment that provides a sufficient air exchange rate.

Warning: To prevent burn injuries, allow sufficient time for the column manager to cool before opening the device’s door. The heater compartment, columns, column trough, preheater assembly, tubing, fittings, and door liner can be extremely hot.

Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use.

98

the underlined value. You cannot edit any underlined values (temperature set point and column selection) while the system is running samples.

Resolving column compartment leak sensor errors

After approximately 1.5 mL of liquid accumulates in the leak sensor reservoir, an alarm sounds, indicating that the leak sensor detected a leak.

Tip: Small column leaks can be undetectable because they can evaporate before reaching the leak sensor reservoir.

Required materials

• Cotton swabs


• Nonabrasive, lint-free wipes

To resolve a column heater leak sensor error (door in closed position):

1. In the ACQUITY UPLC Console’s Leak Sensors dialog box, determine which of the sample manager’s two leak sensors detected a leak.

2. If the message reads “Leak Detected (Column)”, open the column heater’s door. Otherwise, see the “Resolving leak sensor errors” section in the Sample Manager Operator’s Overview and Maintenance Information document.

3. Locate the source of the leak, and make the repairs necessary to stop it.

Tip: Liquid from a detector leak can accumulate in the reservoir of the column heater’s leak sensor, causing an alarm to sound. Always inspect the detector when attempting to locate the source of a leak.

Warning: To avoid personal contamination from contact with biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Caution: To avoid scratching or damaging the leak sensor• do not allow buffered solvents to accumulate and dry on it;• do not submerge it in a cleaning bath.

Using the column compartments 99

4. Disconnect the column inlet, active preheater, or column stabilizer tubing from injector port 6 of the sample manager.

5. Disconnect the column-outlet PEEK tubing from the detector inlet.

6. Remove any solvent tubing routed behind the column heater door.

7. Power-off the sample manager, and detach the leak sensor connector from the front of the column heater.


Location of column, active preheater, or column-stabilizer-inlet tubing

TORX screw

eCord receptacle

100

9. Gently pull the right-hand side of the column heater toward you, and then slide it rightward, to unseat the tab on the left-hand side of the column heater from the instrument chassis.

10. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it, tilting it to the left-hand side.

Tip: If you cannot easily manipulate the leak sensor after removing it from its reservoir, power-off the sample manager, and then detach the connector from the front of the column heater (see page 115).

Caution: To avoid damaging the leak sensor, do not grasp it by the ribbon cable.

Serrations


11. Use a nonabrasive, lint-free wipe to dry the leak sensor prism.

12. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area.

TP02891

Prism

Lint-free wipe

Rolled up lint-free wipe

Leak sensor reservoir

102

13. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area.

Cotton swab



14. Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place.

15. If you detached the connector from the front of the instrument, reattach it, and power-on the sample manager.

16. In the ACQUITY UPLC Console, select Sample Manager FTN from the system tree.

17. In the sample manager information window, click Control > Reset SM, to reset the sample manager.

To resolve a 30-cm column heater leak sensor error:

1. In the ACQUITY UPLC Console’s Leak Sensors dialog box, determine which of the sample manager’s two leak sensors detected a leak.

2. If the message reads “Leak Detected (Column)”, open the 30-cm column heater’s door.


TP02892

T-bar

Leak sensor installed in reservoir

Slot in leak sensor reservoir

104

4. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it.



Serrations

TP02891

Prism

Lint-free wipe




Rolled up lint-free wipe


Cotton swab


106




To resolve a column manager leak sensor error:

1. Power-off the column manager.

2. Open the column manager door.


Tip: Liquid from a detector leak can accumulate in the column manager leak sensor reservoir and cause the column manager leak sensor alarm to sound. Always inspect the detector when attempting to locate the source of a leak.

TP02892

T-bar




4. Detach the leak sensor connector from the front of the column manager by pressing down on the tab and pulling it out of the receptacle.

5. Remove the leak sensor from its reservoir, grasping it by its serrations, and pulling upward.





Leak sensor connector

Press down on tab to release connector

TP02891

Prism

Lint-free wipe

108


10. Reattach the connector to the receptacle on the front of the instrument, and power-on the column manager.

TP02892


T-bar


Leak sensor connector receptacle


11. In the ACQUITY UPLC Console, select Column Manager from the system tree.

12. In the column manager information window, click Control > Reset CM, to reset the column manager.

Maintaining the column compartments

Under normal operating conditions, the column compartments do not require any routine maintenance. If residue from solvent leaks accumulates in the column compartment, remove the column and wipe the compartment clean with a soft cloth.

Contacting Waters technical service

If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800 252-4752). From elsewhere, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. Waters’ Web site includes phone numbers and e-mail addresses for Waters locations worldwide. Visit www.waters.com.

When you contact Waters, be prepared to provide this information:

• Error message (if any)

• Nature of the symptom

• Instrument serial numbers (see page 111)

• Flow rate

• Operating pressure

• Solvent(s)

• Detector settings (sensitivity and wavelength)

• Type and serial number of column(s)

• Sample type

• Data application version and serial number

• ACQUITY UPLC system workstation model and operating system version

For complete information on reporting shipping damages and submitting claims, see the document Waters Licenses, Warranties, and Support Services.

110

Locating system serial numbers

Each system instrument’s unique serial number facilitates service and support. Serial numbers also provide a way to create single log entries for each instrument so that you can review the usage history of only that instrument.

Be prepared to provide the serial numbers of the instruments in your system when you contact Waters customer support.

To view instrument information:

1. In the ACQUITY UPLC Console, select an instrument from the system tree.

2. Click Configure > View module information. The Module Information dialog box displays this information:

• Serial Number

• Firmware Version

• Firmware Checksum

• Component Software Version

Note: ACQUITY UPLC 30-cm CHC information cannot be displayed via the ACQUITY UPLC Console.

Alternatives:

• From the main window, place the pointer over the visual representation of the system instrument you want information for.

• Obtain the serial number from the printed labels on the rear panels of instruments or inside their front doors.

To view column information:

1. In the ACQUITY UPLC Console, select Column from the system tree.

2. View the column part and serial numbers on the Column screen.

Alternative: View column part and serial numbers on the column.

Maintaining the column compartments 111

Maintenance schedule

Waters recommends that you perform the following routine maintenance on the column compartments to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents such as buffers, perform these maintenance tasks more frequently.

Recommended routine maintenance schedule:

Maintenance procedure Frequency For information...

Replace the leak sensors on the system instruments

As needed See page 114 and page 119

Replace the column manager switching valve cartridge

As needed See page 122

Replace the column During scheduled preventive maintenance or as needed

See page 125,

Replace the active preheater assembly


Replace the column stabilizer assembly (CH-A only)

During scheduled preventive maintenance or as needed

See page 133

Replace the column in-line filter frit


Replace the column in-line filter unit

After you have used the six filters that accompanied the unit

See page 136

Replace the fuse (ACQUITY UPLC 30-cm CHC only)


Clean the instrument with a soft, lint-free cloth, or paper dampened with water


112

Maintenance considerations

Safety and handling

Observe these warning and caution advisories when you perform maintenance operations on your system.

Proper operating procedures

To ensure your system runs efficiently, follow the procedures on page 14.

Configuring maintenance warnings

Maintenance counters provide real-time usage status information that can help you determine when to schedule routine maintenance for specific components. You can set usage thresholds and maintenance warnings that alert you when a component reaches the designated threshold limit. By setting threshold limits and monitoring these usage counters regularly, you can minimize unexpected failures and unscheduled downtime during


Warning: To prevent burn injuries, allow sufficient time for the column manager to cool before opening the instrument. The heater compartment, columns, column trough, preheater assembly, tubing, fittings, and door liner can be extremely hot.

Warning: To avoid electric shock, do not remove the instrument’s protective panels. The components within are not user-serviceable.



important work. For information on setting maintenance warnings, consult the ACQUITY UPLC Console online Help.

Replacing the column heater’s leak sensor

Perform this procedure if you must replace the leak sensor.

Required materials


• New leak sensor

Required tools


• T20 TORX driver

To replace the column heater’s leak sensor:


2. Disconnect the column inlet, active preheater, or column stabilizer tubing from injector port 6 of the sample manager.


Location of column, active preheater, or column-stabilizer- inlet tubing

114

3. Open the sample manager door, gently pulling its right-hand edge toward you.

4. Press down on the tab to detach the leak sensor connector from the front of the instrument.

5. Disconnect the column-outlet PEEK tubing from the detector inlet.

6. Remove any solvent lines routed behind the column heater door.




TORX screw

eCord receptacle


8. Gently pull the right-hand side of the column heater toward you, and then slide it to the right, to unseat the tab on the left-hand side of the column heater from the instrument chassis.

9. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it, tilting it to the left.

10. Unpack the new leak sensor.


Serrations

116


12. Plug the leak sensor connector into the front of the instrument.

TP02909

TP02892


T-bar



13. Insert the tab on the left-hand side of the column heater into the slot on the left-hand side of the instrument chassis.

14. Hold the left-hand side of the column heater into the slot, and guide the eCord receptacle tab on the right-hand side into the cutout in the instrument chassis.

15. Install and tighten the TORX screw located behind the eCord receptacle.

Tab

Slot

Tab

Cutout

118

16. Connect the column inlet, active preheater, or column stabilizer tubing to injector port 6 of the sample manager.

17. Connect the column-outlet PEEK tubing to the detector inlet.

18. Power-on the sample manager.



Replacing the 30-cm column heater’s leak sensor

Only a Waters technical service representative should replace the 30-cm column heater’s leak sensor. Contact Waters Technical Service department for more information.

Replacing the column manager’s leak sensor

Perform this procedure if you must replace the leak sensor in the column manager or the auxiliary column manager.

If your system contains only a column manager or both a column manager and one auxiliary column manager, the leak sensor is located on the column manager. If your system contains a column manager and two auxiliary column managers, the leak sensor is located on the lower auxiliary column manager.

The leak sensor is located in the column manager or auxiliary column manager module, which is itself located at the lowest position in the system stack.

Location of column-stabilizer-inlet tubing


Required materials


• New leak sensor

To replace the column manager’s or auxiliary column manager’s leak sensor:

1. Power-off the column manager.

2. Open the column manager door.

3. Press down on the tab, to detach the leak sensor connector from the front of the instrument.

4. Remove the leak sensor from its reservoir, grasping it by its serrations, and pulling upward on it.

5. Unpack the new leak sensor.





120


TP02892


T-bar



7. Plug the leak sensor connector into the receptacle on the front of the instrument.

8. Power-on the column manager.


10. In the column manager information window, click Control > Reset CM, to reset the column manager.

Replacing the column manager switching-valve cartridge

Before replacing the switching-valve cartridge, ensure you have the correct part for your system type (ACQUITY regular or Bio).


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the column manager switching-valve cartridge.

Leak sensor connector receptacle

Front of instrument

122

Required materials


• Switching-valve cartridge

Required tools


• 2-mm Allen wrench (startup kit)

To replace the column manager switching-valve cartridge:


2. In the column manager information window, click Interactive Display.

3. In the column manager interactive display dialog box, click Control.

4. Ensure the switching-valve is set to Waste.

Tip: To change the setting to Waste, click System and select Waste.

5. Label the tubing connected to the switching-valve.

6. Pull the valve forward, 1/2 to 3/4 inches, and rotate the lock ring clockwise until it rests against the lock ring stop.

7. Use the 1/4-inch wrench to remove the fittings attached to the switching-valve cartridge.

Switching-valve cartridge:

2-mm hex screwLock ring stop

Lock ring

Stator ID


8. Use the 2-mm Allen wrench to remove the hex screw at the 10 o’clock position on the switching-valve cartridge.

9. Remove the switching-valve cartridge from the switching-valve assembly by pulling straight forward.

10. Unpack the replacement switching-valve cartridge.

11. Ensure that the groove in the cartridge housing aligns with the groove on the drive clamp.

Requirement: If the grooves fail to align, turn the drive clamp until they do so, taking care to avoid scratching the drive clamp or housing.

12. Insert the new switching-valve cartridge into the switching-valve cartridge chamber.

Requirement: Ensure the new cartridge assumes the same orientation as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service.

13. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm Allen wrench.

14. Pull the valve forward, 1/2 to 3/4 inches, and rotate the lock ring clockwise until it rests against the stopper.

15. Use the 1/4-inch wrench to reattach all fittings and tighten them 1/4-turn beyond finger-tight for existing fittings, or 3/4-turn beyond finger-tight for new fittings.

16. Rotate the lock ring counterclockwise to the 12 o’clock position.

17. Push the switching-valve assembly in until it stops.

Switching-valve cartridge housing

Drive clamp

Aligned grooves

124

Replacing the column

Required materials


• Replacement column

To remove the column:

1. Stop any running mobile phase.

2. After allowing sufficient time for cooling, open the column heater, column manager, or auxiliary column manager door.

3. Do these things on the column manager or auxiliary column manager:

a. Push the latches on top of the column compartment cover inward, to disengage them from the latch pins.


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the column.


b. Tilt the column compartment cover forward just enough to clear the valve drip tray.

c. Pull the column compartment cover up and away from the column manager or auxiliary column manager.

4. Do these things if the column compartment has an active preheater:

a. Remove the active preheater assembly from the retainer.

b. While holding the column, disconnect the column outlet tubing.

Latches

Latch pins

Valve drip tray


Retainer


126

c. While holding the gold active preheater fitting, rotate the column off the fitting.

Requirement: If you are installing the active preheater in a system with pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), use the 1/4-inch and 5/16-inch open-end wrenches to disconnect the compression fitting that attaches the active preheater assembly to the optional in-line filter or to the column.

5. Do these things if the column compartment has a column stabilizer:

a. Disconnect the outlet of the column stabilizer tubing from the column or in-line filter.

b. Place the forked end of the collet separator over the collet on the column stabilizer tubing, and gently move it back and forth to pry the reusable fitting loose from the collet.

Older, reusable fittings do not have holes in their sides.

c. Replace the ferrule.

6. Continue with the instructions on page 89.

Replacing the active preheater assembly

Before replacing the active preheater, ensure you obtained the correct replacement part for your system type (ACQUITY regular or Bio) and column compartment type. Note that the length of the active preheater inlet tubing varies.

If you use the active preheater when running an HPLC or UPLC method originally developed for use with a column stabilizer, a column temperature adjustment can be necessary.

TP02914Collet separator

Reusable fitting

Tubing from column stabilizer or injector

Ferrule

Collet


Required materials


• Replacement active preheater assembly

Required tools



To remove the active preheater assembly:

1. Stop any running mobile phase before replacing the active preheater assembly.

2. After allowing sufficient time for the instrument to cool, open the column heater, column manager, or auxiliary column manager door.

3. Disconnect the inlet tubing:

• Column heater – disconnect the tubing from injector port 6 of the sample manager.

• 30-cm column heater – disconnect the tubing from injector port 6 of the sample manager.

Warning: To prevent burn injuries, set the column temperature to Off, and then allow the column compartment and its components to cool for 60 minutes before touching them. Monitor the column compartment’s internal temperature to ensure all components are cool.

Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the active preheater assembly.

Caution: To avoid damaging the active preheater assembly,• turn the column, not the fitting, when installing or removing the

column to prevent the active preheater tubing from turning with the fitting.

• do not grasp and turn the assembly.• do not suspend or hang the active preheater tubing.

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• Column manager – disconnect the tubing from the appropriate injector port of the switching-valve. The port varies depending on which active preheater you are replacing. See the figures on page 46, page 69, page 70, page 71, and page 72.

• Auxiliary column manager – disconnect the tubing from the appropriate injector port of the switching-valve, located in the column manager. See the figures on page 46, page 69, page 70, page 71, and page 72.

4. Follow these steps for a column manager or auxiliary column manager:

a. Push the latches on top of the column compartment cover inward, to disengage them from the latch pins.

b. Tilt the column compartment cover forward just enough to clear the valve drip tray.

c. Pull the column compartment cover up and away from the column manager or auxiliary column manager.

Latches

Latch pins

Valve drip tray



5. Remove the active preheater assembly from the retainer.

6. While holding the column, disconnect the column outlet tubing.

7. While holding the gold active preheater fitting, rotate the column off the fitting.

Requirement: If you are installing the active preheater in a system with pressures exceeding 103,421 kPa (1034 bar, 15,000 psi), use the 1/4-inch and 5/16-inch open-end wrenches to disconnect the compression fitting that attaches the active preheater assembly to the optional in-line filter or to the column.

To install the new active preheater assembly:

1. Loosen the stainless steel cap nut from the gold fitting.

2. Finger tighten the gold fitting, with the ferrule, into the column.

3. Holding the active preheater’s dual-threaded fitting in place, rotate the column or optional in-line filter onto the fitting until it is snug, and then tighten an additional 3/8-turn.

Tip: For information on installing system fittings, see the table on page 15.

Retainer


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4. Install the new active preheater assembly in the retainer.

If you are replacing the active preheater in a 30-cm column heater:

• Install the active preheater in the correct position for your application.

– UPLC column – install the active preheater in the upper retainer

– HPLC column – install the active preheater in the lower retainer

• Install the retainer cover in the unused active preheater position.

5. Carefully route the active preheater assembly inlet tubing to the valve:

• Column heater – route the tubing through the groove on the side of the column compartment, and then through the cutout in the top of the sample manager door to the injector. Do not route the tubing behind the sample manager door hinge.

• 30-cm column heater – route the tubing through the column heater door.

Warning: Never connect active preheaters in the upper and lower positions at the same time. Doing so can damage the 30-cm column heater.

Active preheater inlet tubing



• Column manager – route the tubing between the column compartment and the side of the sample manager’s chassis, upward, to the switching valve.

• Auxiliary column manager – route the tubing between the column compartment and the side of the instrument chassis to the switching valve.

6. Remove the O-ring retainer from the inlet end of the active preheater assembly tubing, and then connect the inlet tubing to the valve:

• Column heater – connect the tubing to injector port 6 of the sample manager.

• 30-cm column heater – connect the tubing to injector port 6 of the sample manager.

• Column manager – connect the tubing to the appropriate injector port of the switching-valve. The port varies depending on which active preheater you are replacing. See the figures on page 46, page 69, page 70, page 71, and page 72.

• Auxiliary column manager – connect the tubing to the appropriate injector port of the switching valve, located in the column manager. The port varies depending on which active preheater you are replacing. See the figures on page 46, page 69, page 70, page 71, and page 72.

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Replacing the column stabilizer assembly (CH-A only)

Use the column stabilizer only with the column heater (CH-A). Do not use it with the column manager or auxiliary column manager.

Required materials


• Replacement column stabilizer assembly

Required tools




To replace the column stabilizer assembly:

1. Stop any currently running mobile phase before replacing the column stabilizer assembly.


Warning: To prevent electric shock, ensure all waste line tubing is properly connected, and the drip tray is fitted and undamaged. Properly installing the tubing and drip tray keeps waste or solvent from contacting the power cord or cables.

Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the column stabilizer assembly.


2. Disconnect the inlet tubing from injector port 6 of the sample manager.


4. Using the Phillips screwdriver, loosen the two screws that fasten the column stabilizer assembly to the column compartment tray.

5. Remove the column stabilizer assembly from the thermal gasket.

6. Using the 1/4-inch and 5/16-inch open-end wrenches, disconnect the compression fitting that attaches the column stabilizer assembly to the optional in-line filter or to the column, and then remove the column stabilizer assembly.

7. Firmly press the replacement column stabilizer assembly into the thermal gasket. Ensure that the thermal gasket uniformly envelops the sides of the column stabilizer and does not bunch beneath it.

Location of column-stabilizer-inlet tubing

TP03425


Threaded holes for Phillips screws

Thermal gasket visible along both sides of stabilizer

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Column stabilizer assembly (two styles):

8. Carefully route the column-stabilizer-inlet tubing through the groove on the side of the column compartment.

9. Insert the Phillips screws into the threaded holes, and tighten them gently using the Phillips screwdriver.

10. Route the column-stabilizer-inlet tubing through the cutout in the top of the sample manager door to the injector.

Requirement: Do not route the tubing behind the sample manager door hinge.

Caution: To avoid stripping threads, do not overtighten screws.

TP03123

Reusable fittings

Inlet tubing to injector

For 150-mm column For 50-mm or 100-mm column


11. Remove the O-ring retainer from the inlet end of the column stabilizer tubing, and then connect the inlet tubing to injector port 6 of the sample manager.

12. Using the 1/4-inch and 5/16-inch open-end wrenches, connect the compression fitting that attaches the column stabilizer assembly to the optional in-line filter or to the column.

Replacing the frit in the in-line filter unit

If your system is fitted with the optional in-line filter frit, follow the instructions below to replace the frit.

Recommendation: Because of wear on the non replaceable compression fittings, replace the in-line filter unit after you use the six frits that accompany the unit.


Caution: To prevent contaminating system components, wear clean, chemical-resistant, powder-free gloves when replacing the frit in the column in-line filter unit.



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Requirement: Stop any currently running mobile phase before replacing the frit in the in-line filter unit.

Required materials


• Replacement frit

Required tools

5/16-inch open-end wrench (2)

To replace the frit in the in-line filter unit:

1. After allowing sufficient time for cooling, open the column heater, column manager, or auxiliary column manager door.

2. Remove the column components (column, in-line filter unit, and active preheater or stabilizer tubing assembly).

Column components:

3. Detach the active preheater or column stabilizer tubing assembly from the column in-line filter unit.

Requirement: Do not detach the in-line filter unit from the column inlet.

TP02873

Flow

In-line filter unitColumn Active preheater or column stabilizer tubing assembly

InletReusable fitting


4. Open the in-line filter unit by placing two 5/16-inch open-end wrenches onto the flats as shown.

5. Remove the in-line filter unit inlet nut, which contains the used frit.

Tips:

• Discard the frit; it cannot be reused.

• Over time, the frit can expand inside the in-line filter inlet nut. If you cannot remove the frit from the inlet nut, discard the inlet nut, and replace both the inlet nut and frit.

6. Insert a new frit, and then reassemble the in-line filter unit using the wrenches.

7. Reattach the active preheater or column stabilizer tubing assembly to the in-line filter unit.

8. Start the mobile phase, examine the assembled components for leaks, and tighten fittings as needed.

9. Return the assembled components to the column compartment.

TP02543

Flow

Place wrenches on flats and rotate to assemble and disassemble.

Outlet assembly Frit Inlet nut

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Replacing the column heater/cooler fuse (ACQUITY UPLC 30-cm CHC)

Column heater/cooler fuse ratings require:

• Two fuses

• 3.15 A, 250 V Fast-Blo, 5 × 20 mm (IEC)

To install or replace the power supply fuse in the column heater/cooler:

1. Ensure that the power switch is in the Off (0) position, and then remove the power cord from the device’s rear panel.

2. Locate the power entry module on the device’s rear panel.

3. Insert your fingers into the fuse holder slots on the device’s rear panel, and exerting a minimum pressure, pull on the spring-loaded fuse holder, removing it from the rear panel.

4. Remove and discard the old fuse.

5. Make sure that the replacement fuse is properly rated for the column heater/cooler.

6. Insert the new fuse into the fuse holder.

Caution: To avoid damaging electrical components, replace fuses with those of the same type and rating.


7. Insert the fuse holder into the receptacle, and gently push until it locks into position.

Tip: A clicking sound indicates the fuse holder is locked into position.

8. Connect the power cord to the rear panel power entry module.

Cleaning the instrument’s exterior (all models)

Clean surfaces of the column heater, column manager, auxiliary column manager, or column heater/cooler with only a soft, lint-free paper or cloth dampened with water.

Observe these requirements when cleaning device surfaces:

• Always ensure the electrical power to the device is interrupted.

• Always use eye and hand protection during the cleaning process.

• Apply the water to a clean cloth only, and then wipe the device.

• Never spray or apply the water directly onto any device surface.

Warning: To prevent burn injuries, adjust the temperature set-point of the column manager to a safe temperature (10 to 30 °C) and allow the unit to attain this set-point temperature before handling components within the column compartment. When the column manager is set at a high temperature, all interior surfaces and components are hot.

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acquity uplc column compartments operator’s overview and … · 2012-01-17 · overview 1...

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