acquity uplc i-class ivd

ACQUITY UPLCI-Class IVD

Sample Manager - Flow Through Needle Overview and Maintenance Guide

715003737IVD / Revision B

Copyright © Waters Corporation 2013All rights reserved

ii February 26, 2013, 715003737IVD Rev. B

General Information

Copyright notice

© 2013 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

Trademarks

ACQUITY, ACQUITY UPLC, UPLC, Waters Quality Parts, Xevo, and Waters are registered trademarks, and “THE SCIENCE OF WHAT’S POSSIBLE.” are trademarks of Waters Corporation.

Teflon is a registered trademark of E. I. du Pont de Nemours and Company.

TORX is a registered trademark of Textron Corporation.

PEEK is a trademark of Victrex Corporation.

Other registered trademarks or trademarks are the sole property of their owners.

February 26, 2013, 715003737IVD Rev. B iii

Contacting Waters

Contact Waters® with technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow good laboratory practice, and consult your organization’s safety representative for guidance.

Waters contact information:

Contacting medium Information

Internet The Waters Web site includes contact information for Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-HPLC (800 252-4752) or fax 508 872-1990.For other locations worldwide, phone and fax numbers appear in the Waters Web site.

Conventional mail Waters Corporation34 Maple StreetMilford, MA 01757USA

iv February 26, 2013, 715003737IVD Rev. B

Considerations specific to the SM - FTN

FCC radiation emissions notice

Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

Canada spectrum management emissions notice

This class A digital product apparatus complies with Canadian ICES-001.

Cet appareil numérique de la classe A est conforme à la norme NMB-001.

Warning: To avoid injury, if the ACQUITY UPLC I-Class IVD system must be relocated, drain and remove all liquids, including solvents and waste, from the system.

Warning: To avoid injury, if the ACQUITY UPLC I-Class IVD system must be relocated, disconnect the power cord and all cables.

Warning: To avoid skeletal or muscle injury, at least two people must lift the Sample Manager-Flow Through Needle (SM-FTN).

Warning: To prevent incorrect reporting of results, verify proper sample vial placement and positioning against the sample list, prior to sample injection.

Warning: To avoid eye injury, and to prevent personal contamination with biohazards and toxic compounds, ensure that the fluidics compartment door is closed whenever the pumps of the binary solvent manager are pumping solvents. If a leak develops in the fluidics plumbing, pressurized solvent can be released.

February 26, 2013, 715003737IVD Rev. B v

Electrical power safety notice

Do not position the instrument or device so that it is difficult to operate the disconnecting device.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken.

Equipment misuse notice

If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution advisories.

Operating the sample manager

When operating the sample manager, follow standard quality control (QC) procedures and the guidelines presented in this section.

Applicable symbols

Symbol Definition

Indicates the medical device manufacturer as defined in EU Directive 98/79/EC

Indicates the authorized representative in the European Community

Confirms that a manufactured product complies with all applicable European Community directives

vi February 26, 2013, 715003737IVD Rev. B

Australia C-Tick EMC compliant

Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements

Consult instructions for use

Symbol Definition

February 26, 2013, 715003737IVD Rev. B vii

Audience and purpose

This guide is intended for only professionally trained and qualified laboratory personnel who operate and maintain ACQUITY UPLC I-Class IVD solvent managers.

Intended use of the solvent managers.

Waters designed the ACQUITY UPLC I-Class IVD solvent manager for use with the ACQUITY UPLC I-Class IVD system to perform ultra-performance liquid chromatography for in vitro diagnostic use.

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should include the entire range of QC samples, typical specimens, and atypical specimens.

When calibrating mass spectrometers, consult the instrument’s online Help system for instructions.

Quality control

Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. If trays are the same or very similar, vary the location of the QC samples in the trays.

Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste.For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions.

For in vitro diagnostic use

Symbol Definition

viii February 26, 2013, 715003737IVD Rev. B

Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily.

When analyzing samples from a complex matrix such as soil, tissue, serum/plasma, whole blood, and other sources, note that the matrix components can adversely affect LC/MS results, enhancing or suppressing ionization. To minimize these matrix effects, Waters recommends you adopt the following measures:

• Prior to the instrumental analysis, use appropriate sample pretreatment such as protein precipitation, liquid/liquid extraction (LLE), or solid phase extraction (SPE) to remove matrix interferences.

• Whenever possible, verify method accuracy and precision using matrix-matched calibrators and QC samples.

• Use one or more internal standard compounds, preferably isotopically labeled analytes.

ISM classification

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network.

February 26, 2013, 715003737IVD Rev. B ix

EC authorized representative

Waters Corporation (Micromass UK Ltd.)Floats RoadWythenshaweManchester M23 9LZUnited Kingdom

Telephone: +44-161-946-2400

Fax: +44-161-946-2480

Contact: Quality manager

x February 26, 2013, 715003737IVD Rev. B

Table of Contents

General Information .................................................................................... iii

Copyright notice .................................................................................................. iii

Trademarks ........................................................................................................... iii

Contacting Waters ............................................................................................... iv

Safety considerations .......................................................................................... iv Considerations specific to the SM - FTN............................................................ v FCC radiation emissions notice .......................................................................... v Canada spectrum management emissions notice .............................................. v Electrical power safety notice ............................................................................ vi Safety hazard symbol notice............................................................................... vi Equipment misuse notice ................................................................................... vi Safety advisories ................................................................................................. vi

Operating the sample manager ........................................................................ vi Applicable symbols ............................................................................................. vi Audience and purpose...................................................................................... viii Intended use of the solvent managers. ........................................................... viii Calibrating ....................................................................................................... viii Quality control ................................................................................................. viii

ISM classification ................................................................................................. ix ISM Classification: ISM Group 1 Class B ......................................................... ix

EC authorized representative ............................................................................ x

1 Sample manager-flow through needle ............................................... 15

Location of SM-FTN in the ACQUITY UPLC I-Class IVD system ........... 15

Flow path through the sample management system ................................. 17

SM-FTN major components ............................................................................. 18

Functional systems ............................................................................................ 20 R positioning mechanism................................................................................ 20

February 26, 2013, 715003737IVD Rev. B xi

Injection system ................................................................................................. 21

Injection mechanics .......................................................................................... 21 Wash system ...................................................................................................... 23 Thermal system ................................................................................................. 24

2 Preparing for operation ........................................................................ 25

Installing the leak sensor ................................................................................. 26 Required materials ............................................................................................ 26

Installing the waste tubing .............................................................................. 27 Required material .............................................................................................. 27

Calibrating the needle’s z axis ........................................................................ 29

Priming the SM-FTN ......................................................................................... 30

3 Using the SM-FTN ................................................................................... 33

Interface requirements ..................................................................................... 34 Ventilation.......................................................................................................... 34 Drainage system ................................................................................................ 34

Installation recommendations for fittings ................................................... 35 Required material .............................................................................................. 35

Selecting purge and wash solvents ................................................................ 37 Purge solvent...................................................................................................... 37 Wash solvent ...................................................................................................... 37 General guidelines ............................................................................................. 37

Washing the SM-FTN needle ........................................................................... 39

Loading sample plates ...................................................................................... 40 Vial and plate guidelines................................................................................... 40 Required material .............................................................................................. 41

Sample chamber considerations .................................................................... 44

Choosing needles and extension loops ......................................................... 44 Sample needles .................................................................................................. 44 Extension loops .................................................................................................. 44

xii February 26, 2013, 715003737IVD Rev. B

Modifying needle and extension loop configuration parameters..................... 46

Choosing the sample syringe .......................................................................... 47 Modifying sample syringe configuration parameters ...................................... 47

Choosing the sample syringe draw rate ....................................................... 47

Choosing the needle height setting ............................................................... 48

Recovering maximum sample from vials ..................................................... 49

Revising a plate type ......................................................................................... 50

Air gaps ................................................................................................................. 50

Load Ahead and Loop Offline options .......................................................... 51 Load Ahead ........................................................................................................ 51 Loop Offline........................................................................................................ 53 Determining the correct time to take the loop offline...................................... 53 Reducing carryover ............................................................................................ 54

Auto dilution ....................................................................................................... 55

Diagnostic tests .................................................................................................. 56

Resolving leak sensor errors ........................................................................... 56 Required materials ............................................................................................ 57

4 Maintaining the SM-FTN ....................................................................... 61

Maintenance schedule ...................................................................................... 61

Spare parts .......................................................................................................... 63

Troubleshooting with Connections INSIGHT ............................................. 63

Safety and handling .......................................................................................... 64

Contacting Waters technical service ............................................................. 65 Locating system serial numbers ....................................................................... 65

February 26, 2013, 715003737IVD Rev. B xiii

Configuring maintenance warnings .............................................................. 66

Replacing the leak sensor ................................................................................ 67

Replacing the seal .............................................................................................. 69 Required materials ............................................................................................ 69

Replacing the sample needle and needle guide .......................................... 81 Required materials ............................................................................................ 81

Replacing the sample syringe ......................................................................... 89 Required materials ............................................................................................ 89

Cleaning the injection port ............................................................................. 92 Required materials ............................................................................................ 92

Replacing the injection valve cartridge ....................................................... 95 Required materials ............................................................................................ 95

Cleaning the exterior of the SM-FTN ............................................................ 97

A Safety Advisories .................................................................................... 99

Warning symbols .............................................................................................. 100 Specific warnings ............................................................................................. 101

Caution advisory .............................................................................................. 103

Warnings that apply to all Waters instruments and devices ................. 104

Warnings that address the replacing of fuses ........................................... 109

Electrical and handling symbols .................................................................. 111 Electrical symbols ............................................................................................ 111 Handling symbols ............................................................................................ 112

xiv February 26, 2013, 715003737IVD Rev. B

1 Sample manager-flow through needle

You can submit samples for analysis on the ACQUITY UPLC I-Class IVD system by loading microtiter plates or vials onto the rotary sample tray of the Sample Manager-Flow Through Needle (SM-FTN). Using a flow-through-needle mechanism, in which the needle is part of the high-pressure sample flow path, the Sample Manager injects the samples it draws from the plates and vials onto a chromatographic column.

An optional, 50-µL extension loop (installed between the sample needle and the injection valve) increases the volume of injections beyond that of the sample needle. Using the SM-FTN, you also can dilute injected samples (auto-dilution).

Location of SM-FTN in the ACQUITY UPLC I-Class IVD system

The following diagram shows the location of the SM-FTN in the ACQUITY UPLC I-Class IVD system:

Contents:

Topic Page

Location of SM-FTN in the ACQUITY UPLC I-Class IVD system 15

Flow path through the sample management system..................... 17

SM-FTN major components............................................................. 18

Functional systems .......................................................................... 20

Injection mechanics.......................................................................... 21

February 26, 2013, 715003737IVD Rev. B 15


ACQUITY UPLC I-Class IVD System:

Important:

• Use care when stacking or moving the SM-FTN. Ensure the drip tray does not collide with any surface.

• Use care when installing and removing reusable fittings.

See also: AQUITY UPLC Column Heater I-Class IVD Overview and Maintenance Guide (part number 715003738IVD) on the ACQUITY UPLC I-Class IVD System Documentation CD (part number 715003740IVD) to avoid potential leaks or carryover.

Warning: To avoid skeleton or muscle injury, at least two people must lift the Sample Manager-Flow Through Needle (SM-FTN).

Column heater CH-A

Sample manager - FTN

Binary solvent manager

Xevo TQ-S

16 February 26, 2013, 715003737IVD Rev. B

Flow path through the sample management system

Flow path through the sample management system

The following diagram shows how the SM-FTN functions as part of the ACQUITY UPLC I-Class IVD system:

Drainage from column heater

Wash solvent direct from solvent bottle

Sample

Degassed solvent from solvent manager

Inject valve

Mobile phase from solvent manager

To column

Secondary drainage to solvent manager

Secondary drainage to solvent manager

Primary drainage to solvent manager

Primary drainage to solvent manager

Sample syringe valve



SM-FTN major components

Front view with doors closed:

Front view, with doors open:

On/off switch

Power LED

Run LED

Sample compartment door Fluidics compartment door

Plate selector switch

Sample syringe

Location of sample manager leak sensor

Injection valve

Sample tray

Chamber temperature sensor

Access panel


Location of back pressure regulator

Location of column heater leak sensor


SM-FTN major components

Sample compartment components visible with access panel removed:

SM-FTN components:

Component Description

Access panel Removable panel (requires a TORX® driver) that allows access to sample compartment components such as the seal assembly and needle carriage.

Compartment lighting

LED that illuminates the sample compartment. The compartment light automatically turns on when the SM-FTN’s sample compartment door is opened and turns off when the door is closed. The light can also be turned off via the console to accommodate light-sensitive samples.

Injection/wash port An assembly that houses the injection port, needle seal, needle wash mechanism, and force sensor.

Location of sample needle

Injection/wash port

Injection port wash drain

Sample needle carriage

Location of wash tube

Compartment lighting

Location of column heater leak sensor cable connector

Location of sample manager leak sensor cable connector



Functional systems

R positioning mechanism

The R(R-theta) positioning mechanism’s two axes control the orientation of the sample plates within the sample compartment and the relative position of the sample needle carriage.

• The theta-rotary axis is a belt-driven shaft that rotates a pair of sample plates 360° from a reference point.

• The R-linear axis is the axis along which the sample needle carriage is oriented. The carriage runs from the rear-left corner to the front-right corner of the sample compartment.


Directs the needle wash to waste.

Injection valve A two-position, six-port injection valve.

Leak sensor Continuously monitors the SM-FTN for leaks and stops the system flow when its optical sensor detects about 1.5 mL of accumulated, leaked liquid in its surrounding reservoir.

Plate selector switch Toggle switch used to select either plate position 1 or 2.

Sample syringe Draws sample into the sample needle.


A three-position, rotary-shear valve.

Sample needle Extracts sample from vials.

Sample needle carriage

Positions the sample needle in the sample compartment.

Sample tray Secures the sample plates or vial holders in place.

Chamber temperature sensor

Located on the upper guide rail toward the back of the sample chamber, to monitor sample environment.

Wash tube Delivers wash solvent to the inject/wash port.

SM-FTN components: (Continued)

Component Description


Injection mechanics

Injection system

The injection flow path includes the assemblies required to aspirate a sample and deliver it to the column. The process involves the needle, optional extension loop, sample syringe and syringe valve, injection valve, and injection/wash port.

Flow path diagram:

Injection mechanics

During an injection:

1. The needle is positioned and driven down by the R-carriage.

2. A PEEK support sleeve prevents buckling and helps ensure proper alignment with the seat.

Solenoid valve

Wash pump

InOut

Wash solvent

Purge solvent

SampleTo waste

Sample syringe

Pressure transducer

To waste

Union

Inject valve inject position

From solvent manager

To column

Injection port

Sample compartment



3. The needle is driven into the seat to a specified force to form the first high-pressure seal.

4. The second high-pressure seal on the seat is formed during assembly when the seat is locked into place between the support sleeve and overflow cup via a compression nut.

5. The entire injection port assembly rests on a spring and is guided in an aluminum housing.

6. The needle presses against the seat, compressing the spring, which creates the required load between the needle and the seat.

Cross-view of injection needle and seal:

High-pressure seal

Needle motion

Seal

Location of Force sensor

Spring cup

Aluminum housing

Overflow cup

Force applied through the needle carriage

NEEDLE

Needle

Seal


Injection mechanics

The following figure shows a standard ACQUITY UPLC I-Class IVD system cycle time and Load Ahead cycle time:

Standard ACQUITY UPLC I-Class IVD system cycle time definition:

Wash system

The wash system cleans the outside of the sample needle while it is inside the injection/wash port.

You can choose two external needle washes, pre-injection or post-injection. Neither wash sequence allows wash solvent to enter the sample stream.

Pre-injection (insertion) wash

The pre-injection wash washes the needle at a location above the seal position used for injection. The solvent begins to flow before the needle is lowered to this wash position.

Perform this wash if you are concerned about material on the outside of the sample needle damaging the seal or affecting the contact between the seal and the needle.

Start End

SM-FTN sample preparationand positioning

Sample injection

Systemsetup

Standard injection mode

Total ACQUITY UPLC I-Class IVD system cycle time

Chromatographic run time

Pre-wash Post-wash



Post-injection (OD needle) wash

Performed by default, this wash washes the exterior of the sample needle after an injection is made and the needle remains in the seal position.

Two priming modes are available:

• Wash solvent prime, where the wash solvent flows through the wash system to prime each component.

• Purge solvent prime, where the degassed purge solvent flows through the sample syringe.

The purge solvent used for priming is also the solvent used to move sample through the injection flow path. During auto-dilution, the purge solvent is the dilution solvent.

Thermal system

The thermal system maintains the set temperature in the sample compartment.

Tips:

• You do not need to defrost the sample compartment.

• The SM-FTN’s fans stop circulating air whenever the sample compartment door is open.

The sample tray rotates slowly when the system is idle, to help maintain a uniform temperature across the plates.


2 Preparing for operation

Preparation of the SM-FTN involves these steps:

• Installing the leak sensor

• Installing the waste tubing

• Calibrating the rotary sample tray

• Priming the SM-FTN

Before you prepare the SM-FTN for operation, prepare the solvent manager.

Tip: For instructions about preparing the solvent manager, see the ACQUITY UPLC I-Class IVD Binary Solvent Manager Overview and Maintenance Guide (part number 715003739IVD).

Contents:

Topic Page

Installing the leak sensor ................................................................ 26

Installing the waste tubing ............................................................. 27

Calibrating the needle’s z axis ........................................................ 29

Priming the SM-FTN ....................................................................... 30



Installing the leak sensor

Required materials

• Gloves: clean, powder-free, chemical-resistant

• Leak sensor

To install the leak sensor:

1. Power-off the SM-FTN.

2. Open the fluidics compartment door.

3. Carefully unpack the new leak sensor.

Warning: To avoid the harmful effects of personal contact with solvents, including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use.

Warning: To avoid personal contamination with biohazards, toxic compounds, or their residues, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Caution: To avoid damaging electrical parts, never disconnect an electrical assembly while power is applied to an instrument or device. To completely interrupt power, set the power switch to Off, and then unplug the power cord from the AC source. Wait 10 seconds thereafter before you disconnect an assembly.


Installing the waste tubing

4. Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place.

5. Plug the leak sensor connector into the front of the SM-FTN.

6. Power-on the SM-FTN.

7. In the ACQUITY UPLC Console, select Sample Manager FTN from the system tree.

8. In the SM-FTN information window, click Control > Reset SM, to reset the SM-FTN.

Installing the waste tubing

Required material

Gloves: clean, powder-free, chemical-resistant

Warning: To avoid contaminating uncontaminated surfaces with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when installing or removing waste tubing.

TP02892

T-bar

Leak sensor installed in reservoir

Slot in leak sensor reservoir



To install the waste tubing:

1. Locate the pre-installed, corrugated tubing running from the process waste port (found on the lower drip tray of the sample manager), and route it through the pass-through on the upper drip tray of the solvent manager.

2. Slide the adapter onto the end of the corrugated Teflon® tubing.

3. Connect the adapter to the front boss fitting on the lower drip tray of the solvent manager.

Tip: For instructions on routing the solvent manager waste and vent lines, see the ACQUITY UPLC I-Class IVD Binary Solvent Manager Overview and Maintenance Guide (part number 715003739IVD).

Corrugated Teflon tubing

Process waste port

Pass-through on upper drip tray of the solvent manager

Front boss fitting on lower drip tray of the solvent manager


Calibrating the needle’s z axis

Calibrating the needle’s z axis

To calibrate the needle’s z axis:

1. Open the sample manager door.

2. Remove the plates from the trays.

3. In the Console, select Sample Manager > Control > Reset SM.

Result: The mechanism moves to the home position, with the needle above the wash station.

4. Click Maintain > Calibrate needle Z axis.

5. Click Start, and then click OK in the confirmation window.

Tip: To easily and efficiently achieve the correct needle setting, slide a business card under the needle.

6. Select 1.0 mm for displacement per keystroke.

7. Use the +Z button (Page Down key) to drive the needle down to within 1 mm of the tray surface.

8. Switch the displacement increment to 0.1 mm, and lower the needle until it is almost touching the surface of the business card.

9. Click Save > Yes, and then click Close.

10. Remove the card.

11. Specify the sample needle and syringe volumes, if they changed.

12. Characterize the needle seal (see the ACQUITY UPLC online Help).

Caution: To prevent damaging the needle, you must calibrate the it’s z axis before you use the FTN for the first time and thereafter, whenever you replace the sample needle.Improper calibration can result in needle damage if the needle should strike a vial.Additionally, if you are working with limited sample material, and your needle is not properly calibrated, the needle might not aspirate the sample. As a result, peaks will not appear in the resulting chromatogram.



Priming the SM-FTN

The priming process fills the wash system with wash solvent or the injection pathway with purge solvent. You prime the system to accomplish these tasks:

• Prepare a new SM-FTN for operation.

• Prepare a SM-FTN for operation after it has been idle for an extended period.

• Change the purge solvent.

• Remove bubbles from the lines.

Ensure that the purge and wash solvents are correctly composed and that they are high in quality and miscible with any other solvents used in your system. Use filters in all solvent reservoirs, and ensure the volumes of solvents are sufficient for priming.

To prime the sample syringe:


SM-FTN information window:


Priming the SM-FTN

2. Click Control > Prime.

Alternative: Right-click in the SM-FTN control panel, in the data application, and then click Prime.

3. In the Prime dialog box, select the priming function you want to perform.

4. Enter a duration, in seconds, for priming the wash solvent. Enter the number of cycles for priming the purge solvent. Then, click OK.

Recommendation: Specify 5 to 7 primes when you are changing solvents.

Each priming cycle takes approximately 0.5 min. When the system status is “Idle,” priming is finished.

Priming parameter values:

Solvent Range Default

Wash solvent 1 to 600 seconds 15 seconds

Purge solvent 1 to 50 cycles 5 cycles


3 Using the SM-FTN

Before running samples:

• Examine the injection valve, sample syringe, and all fittings for leaks. Tighten fittings as needed.

• Ensure both the sample compartment and the fluidics compartment doors are closed.

Contents:

Topic Page

Interface requirements .................................................................... 34

Installation recommendations for fittings ...................................... 35

Selecting purge and wash solvents ................................................. 37

Washing the SM-FTN needle .......................................................... 39

Loading sample plates ..................................................................... 40

Sample chamber considerations...................................................... 44

Choosing needles and extension loops ............................................ 44

Choosing the sample syringe........................................................... 47

Choosing the sample syringe draw rate.......................................... 47

Choosing the needle height setting ................................................. 48

Recovering maximum sample from vials........................................ 49

Revising a plate type........................................................................ 50

Air gaps............................................................................................. 50

Load Ahead and Loop Offline options............................................. 51

Auto dilution..................................................................................... 55

Diagnostic tests ................................................................................ 56

Resolving leak sensor errors............................................................ 56


3 Using the SM-FTN

Interface requirements

Ventilation

Allow at least 15.2 cm clearance at the rear and at least 1.3 cm clearance on the right-hand side of the SM-FTN for ventilation.

Drainage system

• Allow clearance for fluid lines to pass along the right-hand side of the SM-FTN (twelve 0.125-inch OD tubes or four 1/6-inch OD tubes, for example).

• Ensure that the SM-FTN can accept drainage from the column heater and provide a path to waste.



Installation recommendations for fittings

Installation recommendations for fittings

The system uses gold-plated compression screws and two-piece ferrules. See the diagram below for assembly orientation:

Recommendations:

• To prevent bandspreading, ensure the tubing reaches the bottom of its fitting hole before you tighten the compression screw.

• For easier accessibility, use long compression screws to attach tubes to the injector and vent valve.

• Perform the sample syringe leak test whenever you replace or loosen fittings during maintenance (see the ACQUITY UPLC online Help).

• Whenever you loosen fittings during maintenance, examine them for cracks, stripped threads, and deformations.

• Do not reuse stainless steel fittings more than six times.

Required material

Gloves: clean, powder-free, chemical-resistant

Warning: To avoid personal contamination with biohazards, toxic compounds, or their residues, wear clean, chemical-resistant, powder-free gloves when reinstalling fittings.

Caution: To avoid damaging the active pre-heater assembly, when installing or removing a column, turn the column, not the fitting (compression screw and ferrule). If the fitting turns, the collet could stick, causing tubing of the pre-heater to turn with the fitting.

TubingCompression screwFerrule with locking ring


3 Using the SM-FTN

When tightening system fittings, consult the following table:

Installation recommendations for ACQUITY UPLC fittings:

Fitting Recommended tighteningF

irst

use

or

re-i

nst

alle

d 1/4-28 flangeless with ferrule Finger-tight

Fir

st u

se o

rre

-in

stal

led 1/4-28 flangeless with 2-piece ferrule Finger-tight

Fir

st u

se o

rre

-in

stal

led 10-32 one-piece PEEK Finger-tight

Fir

st u

se

Stainless steel (gold-plated) with 2-piece stainless steel ferrule

Finger-tight, plus 3/4-turn using wrench

Re-

inst

alle

d

Stainless steel (gold-plated) with 2-piece stainless steel ferrule (re-installed)

Finger-tight, plus up to 1/6-turn using wrench

3/4-turn

1/6-turn


Selecting purge and wash solvents

Selecting purge and wash solvents

Purge solvent

Purge solvent is used to move sample along the injection pathway. You also use purge solvent to prime the syringe. The purge solvent does not contact the sample unless it is used as the dilution solvent.

To prevent bacterial growth, use a composition that contains at least some organic modifier. The purge solvent must be compatible with the initial strength mobile phase.

See also: “Priming the SM-FTN” page 30.

Wash solvent

By default, the system washes the exterior of the needle after an injection. You can also prime the wash system with wash solvent to ascertain proper flow through the waste tubing and to confirm that the wash system is operating properly.

Wash solvent is also used in an optional procedure that cleans the exterior of the needle before or after an injection. The wash solvent must solubilize the sample entirely, and it must be compatible with the sample diluent. The wash solvent can contain modifiers.

Take care to select appropriate wash solvents. A poor selection can lead to contamination.

See also: “Wash system” page 23.

General guidelines

For best performance, follow the guidelines in the Solvent Considerations appendix in the ACQUITY UPLC I-Class IVD System Guide (part number 715003736IVD) when selecting purge and wash solvents. Otherwise, you can increase the risk of carryover.

Use purge and wash solvents based on the sample and mobile phase chemistries of your application. When you perform auto-dilutions, ensure purge solvent and sample solutions/buffers are miscible and soluble.

For buffered aqueous, reversed-phase chromatographic conditions and MS applications, it is best to use a wash solvent of 100% methanol or acetonitrile


3 Using the SM-FTN

or a mixture of methanol or acetonitrile with 0% to 20% water. Use a purge solvent with low organic content (~5% to 10%), to minimize dissolved gas while still preventing microbial growth.

If you use the auto-dilution option, for best performance, the purge solvent must be similar or identical to your isocratic or initial gradient solvent conditions, excluding buffers. Do not use salt buffers in purge or wash solvents.

Caution: To avoid damaging the solenoid valve seats and seals in the solvent path, do not use a nonvolatile buffer as the purge or wash solvent.

Wash solvent effects:

Property Effect

Organic species As a general principle, purge and wash solvents must include the same organic species, which is not always practicable. You can, however, use a 100% organic wash solvent.

Solvent composition The purge solvent, if used for auto-dilution, must reflect as closely as possible the same composition as the initial gradient mobile phase.

pH Adjust the pH of the purge and wash solvents for best peak shape and carryover performance.

Concentration of wash solvent

Wash solvent must be no stronger than the concentration needed to reduce carryover to an acceptable level.

Solubility of sample If you are performing auto-dilution, the sample must be soluble in the purge solvent.Note: Proteins (in plasma, for example) do not dissolve in solvents that have an organic component greater than 40%.


Washing the SM-FTN needle

Washing the SM-FTN needle

To wash the SM-FTN needle (OD needle wash):


2. In the SM-FTN information window, click Control > Wash Needle.

Alternative: Right-click the SM-FTN control panel in the data application, and then click Wash Needle.

3. In the Needle Wash box, specify the wash duration, in seconds.

Tips:

• The range is 0 through 99 seconds; the default is 6 seconds.

• The flow rate of the wash solvent is 20 mL/min ± 20%.

4. Click OK.

Result: The needle wash begins. When it ends, the status returns to idle.

To stop a needle wash routine before it finishes:

In the SM-FTN information window, click Control > Reset SM.

Alternative: Right-click the SM-FTN control panel in the data application, and then click Reset SM.

Sample diluent The purge solvent (diluent) will contact the sample, so match the sample matrix as closely as possible. To offset adverse effects on peak shape caused by the matrix’s composition, adjust the purge solvent composition.

Cycle times Higher viscosity wash solvents lengthen wash cycles.

Wash solvent effects: (Continued)

Property Effect


3 Using the SM-FTN

Loading sample plates

The SM-FTN is compatible with the American National Standards Institute (ANSI) well-plates, vial-trays, vials, and cap-mats and their seals approved for use with the ACQUITY UPLC I-Class IVD system. The SM-FTN holds two ANSI/SBS plates that you load via the sample compartment door.

Requirement: The plates you use must meet ANSI/SBS standards. (Waters uses the ANSI standard.)

Vial positions V1 through V4, located on the right-hand and left-hand sides of the sample tray, accommodate 4-mL vials. For inserts that allow you to use 2-mL vials in these positions, contact Waters.

Vial and plate guidelines

Waters recommends that you observe these usage guidelines for sample vials and plates in the SM-FTN:

• Vials

– Use only Waters-certified vials.

– Ensure that vial holders conform to ANSI/SBS standards.

• Plates

– Use only 1860024XX-series plates and cap mats in the SM-FTN.

– When selecting a new plate, especially for 384-well plates, measure the plate size to ensure compatibility with Waters’ specifications for the SM-FTN.

Caution: To avoid incorrect sample identification, always position the sample plate so that the A1 vial poisition occupies the rear, left-hand corner.

Warning: To avoid warping plates, do not centrifuge them.

Warning: Be aware that plates containing samples with high concentrations of organic solvent can give inconsistent results at room temperature.

Warning: Use pre-slit cap-mats/seals and vial caps. Use of non-pre-slit cap mats and vial caps can cause clogging in the wash lines.



• Covers

– Use foil covers on vial plates whenever possible.

– To prevent sample spillage or needle damage, use only Waters-approved covers on the sample vials.

See Also: The Waters Autosampler Vials, Plates and Seals for Use with the AQUITY UPLC Systems Family, part number 720004278, for additional information about plates and vials.

Required material

Safety glasses

To load a sample plate:

1. Open the sample compartment door.

2. Press the plate selector switch on the top, center of the door frame, and select plate position 1 or 2.

Exception: If the SM-FTN is accessing the sample tray when you select a new plate position, the selector switch does not operate, and the device beeps once. The selector switch operates again after the SM-FTN no longer accesses the sample tray.

Tip: Press the plate selector switch twice to toggle between loading a sample plate and loading positions that accept 4-mL vials.

Warning: To avoid eye injury, wear safety glasses when loading sample plates.


3 Using the SM-FTN

3. Pull the sample tray out.

4. Load the plate onto the tray so that well position A,1 is at the rear, left-hand corner, and the forward edge of the plate is behind the spring inside the front of the carrier.

Tip: “A” represents the row number, “1” represents the vial position.

Sample tray pulled out

Position for a 4-mL vial (V1 through V4)

TP03232

Sample plate

A,1 well position



Sample plate vial positions:

5. Slide the tray in until it clicks into place.

Warning: To prevent incorrect reporting of results, verify proper sample vial placement and positioning against the sample list, prior to sample injection.

1 2 3 4 5 6 7 8

A

B

C

D

E

F

Sample tray handle


3 Using the SM-FTN

6. Close the sample compartment door.

Sample chamber considerations

Choosing needles and extension loops

Sample needles

Waters offers multiple needle sizes, so you can choose the best option for your injection volume and sample viscosity. The needles, made from metal, provide greater strength and more robust operation than some other traditional materials. Waters recommends metal needles for samples known to be attracted to hydrophobic polymers.

Tip: Using a smaller needle increases cycle times.

Extension loops

Extension loops increase the volume of sample that can be drawn and held for injection. The 50-µL injection loop is an optional part of the injection system.

Caution: To avoid damaging the sample needle, the sample plates must be positioned correctly and the sample tray must be fully engaged.

Warning: To avoid puncture wounds, keep hands or loose clothing clear of the needle assembly mechanism while it is moving. Note that the SM-FTN beeps three times whenever the sample compartment door is open and the needle assembly mechanism is about to move.

The following metal sample needles are available for the SM-FTN:

Needle sizeRecommended maximum injection volume

15-L (24-inch L 0.007-inch ID) – Default 10 L

30-L (24-inch L 0.010-inch ID) 25 L


Choosing needles and extension loops

It is installed between the needle and the injection-valve port. The maximum injection volume is 50-µL.

Recommendation: Use a sample syringe with a volume of at least two times that of your sample. When using the 50-µL extension loop, you can use the default (100-µL) sample syringe. See “Choosing the sample syringe” page 47 for more information about replacing the sample syringe.

Required materials

• Extension loop kit

• 1/4-inch open-end wrench


To install an extension loop:



3. Remove the sample needle from port 4 of the injection valve.

From injection port

To column


To waste

Sample syringe

Needle or extension loop


3 Using the SM-FTN

4. Screw the extension loop fitting into port 4 of the injection valve, and then use the 1/4-inch open-end wrench to tighten the fitting 3/4-turn past finger-tight.

5. Screw the sample needle fitting into the extension loop union, and then use the 1/4-inch open-end wrench to tighten the fitting 3/4-turn past finger-tight.

Requirement: During system operation, ensure solvent does not leak from the connection between the sample needle fitting and the extension loop. The waste management system cannot collect leaks from this connection.

6. Close the fluidics compartment door.


8. Modify the extension loop volume configuration according to the instructions in page 46.

Modifying needle and extension loop configuration parameters

To configure the system for a needle or extension loop size that differs from the one currently fitted:


2. Select Configure > Volumes.

TP03450

Extension loop union

Extension loop fitting


Choosing the sample syringe

3. In the Volume Configuration dialog box, select the appropriate needle or extension loop size from the list, and then click OK.

Choosing the sample syringe

You can use the following sample syringe sizes in the SM-FTN:

• 100 L (default)

• 250 L

Choose a syringe size that allows you to draw your desired total sample volume into the sample needle (and optional extension loop). Waters recommends using a sample syringe volume that is at least twice the volume of your sample.

Modifying sample syringe configuration parameters

To configure the system for a syringe size that differs from the one currently fitted:


2. Select Configure > Volumes.

3. In the Volume Configuration dialog box, select the appropriate sample syringe size from the list, and then click OK.

Choosing the sample syringe draw rate

The ideal syringe draw rate depends on sample volume, sample viscosity, and desired cycle time. The default syringe draw rates depend on the needle size:

• 15 L needle: 30 L/min

• 30 L needle: 120 L/min


3 Using the SM-FTN

You can also specify the draw rate (in L/min), if desired.

Choosing the needle height setting

The default setting for needle penetration depth prevents you from reaching the bottom of the vial.

You can change the default needle placement (needle height) setting in the software in two places: the Dilution tab of the Sample Manager instrument method editor and the Advanced Settings dialog box. Set consistent values in both places.

To aspirate more sample from the vial, decrease the value so that the needle tip is closer to the bottom of the vial.

Tips:

• The default needle-height setting for 48-vial plates with a 2-mL vial is “automatic”, which is set at 4.0 mm.

• The default needle-height setting for all other plates is 2.0 mm.

Maximum syringe draw rates:

Solvent Type15-L needle (0.007-inch ID)

30-L needle (0.010-inch ID)

50:50 methanol/water 30 L/min 120 L/min

100% water 55 L/min 230 L/min

100% acetonitrile 150 L/min 640 L/min

100% dimethyl sulfoxide (DMSO) 25 L/min 100 L/min

Caution: To avoid damaging the needle, follow the guidelines in this section, and use an appropriate needle-height setting for your sample plates or vials.


Recovering maximum sample from vials

Recovering maximum sample from vials

The current ANSI plate (48 vials) definition for the 2-mL Maximum Recovery Vials can, folowing aspiration, leave some sample in the vial. If you want to recover the maximum amount of sample, change the needle placement setting.

Tip: To modify needle placement for vials, click Instrument Method Editor > ACQ-FTN > General tab > Advanced, and change the “Needle Placement (from bottom)” value.

Vial type

Minimum needle placement (mm)

Description Part number

Waters Supplied Total Recovery Vial

0.7 Screw Cap 12 × 32 mm Clear Total Rec pre-slit PTFE/Silicone Septa

186000385C

Waters Supplied Max. Recovery Vial

2.1 Screw Cap 12 × 32 mm Clear Max Rec pre-slit PTFE/Silicone Septa

186000327C

Screw Cap 12 × 32 mm Amber Max Rec pre-slit PTFE/Silicone Septa

186003886C

Vial depth

Sample needle

2 mm offset


3 Using the SM-FTN

See also: Waters Sample Vials and Accessories brochure on www.waters.com.

Revising a plate type

To select a new plate type:

In MassLynx software, in the Bed Layout area, select a plate.

Result: The plate number is updated. If the plate layout differs from the previous one, the picture is updated.

Air gaps

If air gaps are required for your chromatography, you can specify an air-gap volume in the instrument method editor. The default air-gap volume is 0 L.

To specify an air-gap volume:

1. In the instrument method editor, click the ACQ-FTN tab and then the General tab.

2. Click Advanced.

Waters Supplied Flat Bottom Vial

0.1 Screw Cap 12 × 32 mm Clear with pre-slit PTFE/Silicone Septa

186000307C

Screw Cap 12 × 32 mm Amber pre-slit PTFE/Silicone Septa

186000847C

Screw Cap 12 × 32 mm 750 µL PP pre-slit PTFE/Silicone Septa

186002636

Screw Cap 12 × 32 mm 300 µL PP pre-slit PTFE/Silicone Septa

186002639

Vial type

Minimum needle placement (mm)

Description Part number


Load Ahead and Loop Offline options

3. In the Advanced Settings dialog box, mark the box for the air-gap option.

4. Specify volumes for your pre-aspirate and post-aspirate air gaps, and then click OK.


Load Ahead

When a sample is running, choosing the Load Ahead option causes the sample manager to immediately aspirate the next sample in the sample list. Thus the option reduces an injection’s overhead time (the time beyond what is necessary for chromatographic analysis). In many applications, the Load Ahead option reduces cycle time without degrading chromatographic performance.

Requirement: You must take the needle and optional extension loop off-line when you use the Load Ahead option.

The following figures show a standard ACQUITY UPLC I-Class IVD system cycle time and Load Ahead cycle time:

Caution: To prevent the loss of sample, understand that the Load Ahead option does not operate for the first injection of a sample set or for injection sets whose methods differ.


3 Using the SM-FTN

Standard ACQUITY UPLC I-Class IVD system cycle time definition:

ACQUITY UPLC I-Class IVD system Load Ahead cycle time definition:

Tip: The time buffer delay is a “wait time” that compensates for variations in the time it takes to load a sample.

Start End

SM-FTN sample preparationand positioning

Sample injection

Systemsetup

Standard injection mode



Pre-wash Post-wash

EndStart

Sample injection


Load Ahead mode after the first injection


SM-FTN sample preparation

SM-FTN wash time

Time buffer delay

System setup and sample positioning

Loop comes offline



If the wash time and the sample prep time are equal to or greater than the chromatographic run time, then the Load Ahead option does not improve the cycle time.

Loop Offline

The Loop Offline option can reduce the delay volume. It takes the needle and extension loop offline before the gradient reaches the injection valve and after the sample transfers to the injection port. Nevertheless, dwell volume is significant only in systems that use extension loops larger than 50 µL.

Take the loop offline:

• Before the first gradient change reaches the injection valve

• After the gradient returns to the initial conditions

Determining the correct time to take the loop offline

At the point the loop is eliminated from the flow path, it contains the mobile phase composition. Solutes that deposit in the extension loop owing to poor solubility are not transferred to the column until the gradient composition dissolves the sample and flushes it onto the column. At that point, the solute’s high retention factor (k') causes it to elute from the column in one column volume. Choosing the correct time to take the extension loop offline ensures that all of the peaks are flushed out of the loop.

If you are taking the loop offline before the first gradient change, flush the needle and extension loop with at least five loop volumes of solvent at the initial gradient composition to completely flush the sample onto the column. In this case, use the following formula to calculate the loop offline time:

Example: For a system flowing at 100 µL per minute, with a 50-l loop installed, the loop offline time should be at least 2.5 minutes.

Loop-offline time (min) =

Nominal needle and extension loop volume (L)

Flow rate (L/min) 5


3 Using the SM-FTN

If you are taking the needle and optional extension loop offline after the end of the gradient, ensure they are completely filled with solvent of the initial gradient composition before taking them offline.

In addition, observe these considerations when determining the correct loop offline time:

• If the wash solvent volume has been significantly increased, the washing process may not be completed when the loop switches back. If this is the case, extend the loop offline time.

• The loop-offline time must not occur if the contents of the needle and optional extension loop are of a higher concentration than the initial gradient conditions.

To select the Load Ahead and Loop Offline options:

1. In the instrument method editor, click the ACQ-FTN tab and then the General tab.

2. Select the check boxes for the Load Ahead and Loop Offline options.

3. If you chose the Loop Offline option, specify a time interval.

Tip: In the SM-FTN, a programmed gradient typically flows through all parts of the device that contact the sample. If you initiate the Loop Offline option before the gradient reaches its final conditions, the highly organic portion of the gradient does not pass through the needle. As a result, the gradient can fail to remove all sample from the needle, resulting in low sample recovery and an increased risk of carryover.

Reducing carryover

In a chromatographic system, any substance that creates unwanted peaks or excessive background noise is contamination. Carryover, a specific type of

Loop-offline time (min) =50 (L)

100 (L/min) 5

Loop-offline time (min) = 0.5 min 5

Loop-offline time (min) = 2.5 min


Auto dilution

contamination, occurs when sample material remaining in the system after an injection appears as peaks in subsequent injections, compromising quantification. To optimize system performance, carryover must be minimized and held to an acceptable level (often, below the limits of detection).

Carryover can result from incorrectly installed tubing, fittings, or other hardware or by selecting ineffective wash solvents. Take these actions to reduce carryover:

• Restrict extension loop usage to one system. Owing to part tolerances, fittings that have already been mated to one part may become a source of carryover when used with a different part.

• Ensure all tubing connections are properly set. Tubing must seat properly (that is, without gaps) in its fittings before you tighten them. Poorly seated connections create unnecessary space reservoirs that retain sample, increasing carryover.

• Inspect the needle guide for sample residue or debris, which can cause carryover. If necessary, clean or replace the guide.

• Avoid plate or vial sealing systems that use sticky substances, which can cause carryover.

• If you suspect sample interaction with the needle material, increase the strength of your wash solvent or the wash time. If carryover persists, replace the needle with one of a different material composition, such as the Bio (MP35N) needle, which may reduce sample interaction.

• When selecting wash solvents, follow the guidelines set forth in the Solvent Guidelines appendix of the ACQUITY UPLC I-Class IVD System Guide (part number 715003736IVD).

Auto dilution

Choose the auto-dilution option to dilute dissolved samples (containing no solids) with a solvent the sample syringe delivers. You can specify an interval, to allow time for sample mixing.

To choose the dilution option:

1. In the instrument method editor, click the ACQ-FTN tab and then the Dilution tab.

2. Select Enable dilution.


3 Using the SM-FTN

3. Specify a needle height, purge solvent volume, and a post dilution delay interval.

Diagnostic tests

You can select these diagnostic tests from the SM-FTN’s Maintain menu:

• Needle seal readiness test, which verifies that, when the needle is in the “seal” position and the injection valve is in the “inject” position, no unacceptable drop in solvent pressure occurs.

• Sample syringe leak test, which verifies the sample path is free of leaks.

The Maintain menu also lists these functions:

• Characterizing the needle seal, which determines the seal location.

• Calibrating the needle’s Z-axis, which calibrates the vertical position of the needle.

• Disabling motors, which you do before manually moving the sample tray and R-carriage.

• Parking the sample needle and injection valve, which you do before storing the system, or replacing a needle or valve.

• Replacing the needle, seal, and sample syringe.

See also:

• ACQUITY UPLC I-Class IVD Binary Solvent Manager Overview and Maintenance Guide (part number 715003739IVD) for information on the solvent manager’s leak test.

• ACQUITY UPLC Console online Help for additional information about running diagnostic tests.

Resolving leak sensor errors

The SM-FTN is the only ACQUITY UPLC instrument fitted with two leak sensors, bottom and top. These are called the SM-FTN leak sensor and the column heater leak sensor, respectively.

After approximately 1.5 mL of liquid accumulates in the leak sensor reservoir, an alarm sounds indicating that the leak sensor detected a leak.



Required materials

• Cotton swabs


• Nonabrasive, lint-free wipes

To resolve a leak sensor error:

1. In the ACQUITY UPLC Console’s Leak Sensors dialog box, determine which of the SM-FTN’s 2 leak sensors detected a leak.

2. If the message reads “Leak Detected”, locate the source of the leak, and make the repairs necessary to stop it. If you need additional information, see the Column Heater Operator’s Overview and Maintenance Information document.

3. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it.

Warning: To avoid personal contamination with biohazards or toxic materials, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Caution: To avoid scratching or damaging the leak sensor:• Do not allow buffered solvents to accumulate and dry on the leak

sensor.• Do not submerge the leak sensor in a cleaning bath.

Caution: To avoid damaging the leak sensor, do not grasp it by the ribbon cable.

Serrations


3 Using the SM-FTN

Tip: If you cannot easily manipulate the leak sensor after removing it from its reservoir, detach the connector from the front of the device (see “Replacing the leak sensor” page 67).

4. Use a nonabrasive, lint-free wipe to dry the leak sensor prism.

5. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area.

6. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area.

TP02891

Prism

Lint-free wipe

Rolled up lint-free wipe

Leak sensor reservoir

Cotton swab

Leak sensor reservoir




8. If you detached the connector from the front of the instrument, reattach it.



TP02892

T-bar




3 Using the SM-FTN


4 Maintaining the SM-FTN

Keep to a maintenance schedule and perform maintenance as required and described in this chapter

Maintenance schedule

Waters recommends that you perform the following routine maintenance on the SM-FTN to ensure reliable operation and accurate results. When using the system throughout the day (and on nights and weekends), or when using aggressive solvents such as buffers, perform these maintenance tasks more frequently.

Contents:

Topic Page

Maintenance schedule ..................................................................... 61

Spare parts ....................................................................................... 63

Troubleshooting with Connections INSIGHT ................................ 63

Safety and handling......................................................................... 64

Contacting Waters technical service............................................... 65

Configuring maintenance warnings................................................ 66

Replacing the leak sensor ................................................................ 67

Replacing the seal ............................................................................ 69

Replacing the sample needle and needle guide .............................. 81

Replacing the sample syringe.......................................................... 89

Cleaning the injection port .............................................................. 92

Replacing the injection valve cartridge........................................... 95

Cleaning the exterior of the SM-FTN ............................................. 97



See: For information about how to schedule routine preventative maintenance based on system usage, see page 66.

Recommended routine maintenance schedule:

Maintenance procedure

FrequencyFor information

Clean the SM-FTN with a soft, lint-free cloth, or paper dampened with water

Perform as part of your annual routine scheduled maintenance and when solvent or dust accumulates.

See page 97.

Replace the leak sensor

As needed. For example, when the leak sensor is damaged or an error message states the leak sensor is not detected.

See page 67.

Replace the needle seal

Replace as part of the annual scheduled routine maintenance and, thereafter, as needed. The typical failure modes are as follows:• The dynamic leak test fails.• The needle seal readiness test fails.• Peaks do not appear on your

chromatogram.• Peak areas are lower than expected.

See page 69.

Replace the sample needle and needle guide

Replace when the needle breaks, when the error message “Drawing sample rate excessive <value>” appears, or the needle seal readiness test is failed.

See page 81.

Replace the sample syringe

Replace as part of the annual scheduled routine maintenance and, thereafter, as needed. The typical failure modes are as follows:• The sample syringe leak test fails.• Air bubbles are in the syringe.• A sample syringe hardware error

message appears.

See page 89.


Spare parts

Spare parts

Troubleshooting with Connections INSIGHT

Connections INSIGHT® is an “intelligent” device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system. To use Connections INSIGHT, you must install its service agent software on your MassLynx workstation. In a client/server system, you must also install the service agent, on the computer from which you control the system. The service agent software automatically and securely captures and sends information about the support needs of your system directly to Waters.

If you encounter a performance issue when using the Instrument Console, you can manually submit a Connections Insight request to Waters customer support. Alternatively, you can use Remote Desktop, a real-time collaboration option that controls the two-way connection with the ACQUITY UPLC system by enabling the Connections INSIGHT iAssist service level.

Consult these sources for more information about Connections INSIGHT and Connections INSIGHT iAssist:

• http://www.waters.com

• Connections INSIGHT Installation Guide (part number 715001399)

Replace the injection valve cartridge

Perform as part of your annual routine scheduled maintenance and if the system shows erratic system pressure from run to run.

See page 95.

Clean the injection port

Perform as part of your annual routine scheduled maintenance and if you see sample or particulate accumulation.

See page 92.

Caution: To ensure that your system operates as designed, use only Waters Quality Parts®. Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them.

Recommended routine maintenance schedule: (Continued)

Maintenance procedure

FrequencyFor information



• Connections INSIGHT User's Guide (part number 715001400)

• Your sales representative

• Your local Waters subsidiary

• Waters Customer Support

To submit a Connections Insight request:

1. Select Troubleshoot > Submit Connections Insight request.

2. In the Connections Insight Request dialog box, type your name, telephone number, e-mail address, and a description of the problem.

3. Click Submit, and allow approximately 5 minutes to save the service profile.

Result: A .zip file containing your Connections Insight profile is forwarded to Waters customer support for review.

Tip: Saving a service profile or plot file from the Instrument Console can require as much as 150 MB of file space.

Safety and handling

Bear in mind the following safety considerations when performing maintenance procedures:

Warning: To avoid personal contamination with biohazards, toxic compounds, or their residues, wear clean, chemical-resistant, powder-free gloves while handling the components.

Warning: To prevent injury, always observe Good Laboratory Practice when handling solvents, changing tubing, or operating the instrument. Know the physical and chemical properties of the solvents used (see the Material Safety Data Sheets for the solvents in use).

Warning: To avoid electric shock, do not remove the instrument’s protective panels. The panels cover components that are serviceable only by Waters technicians.

Warning: To avoid electric shock, ensure that the instrument is in Standby mode before beginning any maintenance operation.


Contacting Waters technical service

See Appendix A for safety advisory information.

Contacting Waters technical service

If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800 252-4752). Otherwise, phone the Waters corporate headquarters in Milford, Massachusetts (USA) or contact your local Waters subsidiary. The Waters Web site includes phone numbers and e-mail addresses for Waters locations worldwide. Visit www.waters.com.

When you contact Waters, be prepared to provide this information:

• Error message (if any)

• Nature of the malfunction

• Serial numbers of modules (see page 65)

• Flow rate

• Operating pressure

• Solvent(s)

• Detector settings (sensitivity and wavelength)

• Type and serial number of column(s)

• Sample type

• Data application version and serial number

• ACQUITY UPLC system workstation model and operating system version

For complete information on reporting shipping damages and submitting claims, see the document Waters Licenses, Warranties, and Support Services.

Locating system serial numbers

Each system module bears a serial number that facilitates service and support. Serial numbers also provide a way to create single log entries for each module so that you can review the usage history of a particular unit.




Be prepared to provide the serial numbers of the modules in your system when you contact Waters customer support.

To view module information:

1. In the ACQUITY UPLC Console, select an module from the system tree.

2. Click Configure > View module information.

The Module Information dialog box displays this information:

• Serial number

• Firmware version

• Firmware checksum

• Component software version

Alternatives:

• From the main window, point to the visual representation of the system module for which you want information.

• Obtain the serial number from the printed labels on the device’s rear panel or inside the sample compartment door.

Configuring maintenance warnings

Maintenance counters provide real-time usage status information that can help you determine when to schedule routine maintenance for specific components. You can set usage thresholds and maintenance warnings that alert you to when a component reaches the designated threshold limit.

By setting threshold limits and monitoring these usage counters regularly, you can minimize unexpected failures and unscheduled downtime during important work. For information on setting maintenance warnings, consult the ACQUITY UPLC Console online Help.


Replacing the leak sensor

Replacing the leak sensor

Required materials


• New leak sensor

To replace the leak sensor:


2. Press down on the tab to detach the leak sensor connector from the front of the SM-FTN.


Leak sensor connector

Press down on tab to release connector



3. Remove the leak sensor from its reservoir, grasping it by its serrations, and pull upward on it.

4. Carefully unpack the new leak sensor.


6. Plug the leak sensor connector into the front of the SM-FTN.

Serrations

TP02892

T-bar




Replacing the seal



Replacing the seal

Required materials




• T10 TORX driver

• T20 TORX driver


• Seal kit

To remove the seal:

1. In the ACQUITY UPLC Console, select Sample Manager-FTN from the system tree.

2. Click Maintain > Replace > Seal.

Result: Doing so moves the needle carriage towards the back of the sample compartment.

3. Open the sample compartment and the fluidics compartment doors.

Warning: To avoid personal contamination with biohazards, toxic compounds or their residues, wear clean, chemical-resistant, powder-free gloves when performing this procedure.



4. Using the T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel.

5. Using the 1/4-inch open-end wrench, unscrew the seal port tube’s fitting from port 1 of the injection valve.

Tip: The seal port tube’s fitting is located in the 1 o’clock position.

Captive screw (2)Access panel

Location of seal port tube fitting (port 1)


Replacing the seal

6. Using the T10 TORX driver, remove the screw that secures the seal assembly to the sample compartment floor, lift the assembly upward, and remove it and the seal port tube from the sample compartment.

7. Unscrew the finger-tight wash tube fitting from the finger-tight wash fitting.

8. Unscrew the finger-tight wash fitting from the seal assembly.

Screw

Seal assembly

Wash fitting

Location of wash tube fitting



9. Slide the support sleeve out of the metal housing. Guide the seal port tube through the slots.

Requirement: Hold the assembly in a vertical position when disassembling it. Doing so helps ensure that the metal spring stays in place and in the proper position.

10. Straighten the seal port tube.

11. Place the seal port’s metal housing in its original location on the sample compartment floor.

Result: Doing so helps ensure that the metal spring stays in place and in the proper position.

Note: The metal spring can fall out of the spring cup if the seal port metal housing is not placed it its original location on the sample compartment floor.

12. Place the 1/2-inch open-end wrench on the PEEK support sleeve to hold it in place.

Support sleeve

Metal spring

Slot

Metal housing

Location of seal port tube

Spring cup

Locking nut


Replacing the seal

13. Place the 5/16-inch open-end wrench on the stainless steel locking nut.

14. Loosen the locking nut, and unscrew it from the support sleeve.

Place 1/2-inch open-end wrench here



Support sleeve

Locking nut

Tube/port assembly



15. Remove the seal from the seal port, and then discard the seal.

To install a new seal:

1. Insert the new seal into the seal port. The seal is keyed, ensuring that it will only install one way, as shown below.

Warning: To avoid contaminating uncontaminated surfaces with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when installing or removing waste tubing.

Seal

Locking nut

Seal port

Seal

Locking nut

Smaller diameter end

Seal port

Notch


Replacing the seal

2. Finger-tighten the locking nut into the support sleeve.

3. Place the 1/2-inch open-end wrench on the support sleeve to hold it in place.

Seal

Align notch with pin in seal cup

Pin

Seal cup

Support sleeve

Locking nut



4. Place the 5/16-inch open-end wrench on the locking nut and tighten 1/4-turn beyond finger tight.

5. Bend the line 90 degrees in line with the threaded hole in the support sleeve.

Note: To avoid interfering with the motion of the metal spring in the metal housing, the bend in the seal port tube must not extend beyond the step in the locking nut.

Caution: To avoid damaging the seal port tube, do not excessively twist the tube.



Support sleeve

Step in locking nut

Threaded hole


Replacing the seal

6. Slide the seal port tube into the slot on the side of the metal housing.

Requirement: Ensure the three prongs on the locking nut are seated inside the metal spring.

Prongs on locking nut correctly seated inside metal spring:

Support sleeve

Metal spring

Slot

Metal housing


Spring cup

SpringProng (3)

Locking nut



7. Slide the support sleeve into the metal housing, ensuring the fitting hole on the support sleeve aligns with the slot on the metal housing.

8. Screw the wash fitting finger-tight into the support sleeve.

9. Screw the wash tube fitting into the wash fitting, and tighten it finger-tight plus 1/4-turn.

Caution: To avoid damaging the support sleeve, be careful not to cross-thread the wash fitting.

Fitting hole

Slot

Wash fitting

Location of wash tube fitting


Replacing the seal

10. Adjust the amount of tubing in the sample compartment by sliding it in and out of the foam.

Tip: The wash tube is secured to the wall and should not interfere with the sample tray operation or vertical motion of the wash port.

11. Place the seal assembly in its original location on the sample compartment floor and align the screw hole with the hole in the floor, ensuring the injection port wash drain is in the needle wash basin.

Tip: There are unused holes in the sample compartment floor.

12. Using the T10 TORX driver, tighten the screw that secures the seal assembly to the sample compartment floor.

13. Ensure all cables are routed so they do not interfere with the operation of the needle carriage home sensor.

Using gentle radius bends, route the seal port tube to the right, following straight up the right side edge of the sample compartment. The seal port tube must be routed in the middle of the wash fitting and

Screw

Seal assembly


Needle wash basin



the load cell cable. Ensure the cable exits the injection port assembly without any tight bends in front of the load cell cable.

Important: To avoid errors in operation, ensure that the load cell cable is routed behind the seal port tube and the wash tube.

14. Using gentle radius bends, route the seal port tube behind the needle tube and to the right.

15. Screw the seal port’s fitting into port 1 of the injection valve, and then use the 1/4-inch open-end wrench to tighten the fitting 1/4-turn past finger-tight.

16. Reinstall the access panel using the T20 TORX driver to tighten the two screws that secure the panel to the front of the unit.

Requirement: Ensure that the seal port tube is routed under the gap on the side of the access panel.

Location of seal port tube fitting (port 1)

Access panel gap


Replacing the sample needle and needle guide

17. Close the sample compartment and fluidics compartment doors.


19. Perform the needle seal readiness test (see the ACQUITY UPLC online Help).


Required materials

• T6 TORX driver

• T10 TORX driver

• T20 TORX driver


• Needle assembly kit

To remove the sample needle and needle guide:


2. Click Maintain > Replace > Needle.

Result: This invokes a wizard that moves the sample needle assembly to an accessible position.

3. Open the sample compartment and fluidics compartment doors.




4. Using a T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel.

5. Remove the sample plate if a plate is loaded.

6. Unscrew the needle assembly’s fitting from injector port 4.


Location of needle assembly’s fitting (port 4)



7. Inside the sample compartment, open the needle-tubing guide cover located on the roof of the compartment by rotating it clockwise.

8. Push the needle latch back to release the needle mounting cylinder from its mounting cavity and the needle tubing from its notches.

Guide cover Guide channelNeedle tubing

Needle latch NotchesMounting cavity



9. Lift the needle tip out of the needle guide at the bottom of the needle mechanism.

10. Using a T10 TORX driver, loosen the screw on the needle-tubing clamp and remove the tubing.

Warning: To avoid puncture wounds or damage to the end of the needle, do not touch or press the end of the sample needle.

Caution: To avoid damaging the needle, do not touch the end.

Needle guide

Needle

Remove needle assembly from this clamp

Clamp screw



11. Using the T6 TORX driver, loosen the needle guide set screw, and then remove the needle guide.

To install a new sample needle and needle guide:

1. Install the new needle guide, and tighten the set screw.

2. Holding the new needle assembly with both hands to control its position within the sample compartment, secure the mounting sleeve in the opening of the tubing clamp, with the fitting oriented toward the valve.

ACQUITY UPLC sample needle assembly:

Caution: To avoid letting go of the needle assembly and possibly damaging the tip of the needle, do not tighten the clamp screw during this step of the procedure.

Set screw

Needle guide

Fitting ID sleeveMounting sleeve Needle tip

Mounting cylinder



3. From inside the sample compartment, take hold of the needle assembly tubing as it enters the compartment from above.

4. Ensure the needle tubing is routed to the left side of the needle carriage’s rails and is secured in the guide channel on the roof of the compartment.

5. Remove the protective cap from the needle tip.

6. Insert the needle mounting cylinder into the mounting cavity.

Secure mounting sleeve in this clamp

Guide coverGuide channel Needle tubing



7. Route the tubing through the two notches below the Z-flag.

8. Push the needle latch forward to secure the needle assembly.

9. Using a T10 TORX driver, tighten the screw on the needle-tubing clamp.

Caution: To avoid damaging the SM-FTN, do not place the loose loop of tubing above the Z-flag.

Notches

Mounting cavity

Do not loop needle tubing above Z-flag

Needle latchNotches



10. Insert the needle’s tip into the needle guide at the bottom of the needle mechanism.

11. Ensure that the needle tubing is fully inserted into port 4, on the injection valve, and then thread the fitting into the port, tightening the fitting securely.

12. Reinstall the access panel, using the T20 TORX driver to tighten the two screws that secure it to the front of the unit.

Requirement: Ensure that the seal port tube is routed under the gap on the side of the access panel.


14. Calibrate the needle’s Z axis (see the ACQUITY UPLC online Help).

Insert needle tip here

Access panel gap


Replacing the sample syringe


16. Perform the needle seal readiness test (see the ACQUITY UPLC online Help).


Replace the sample syringe when any of these conditions arise:

• The syringe plunger tip becomes worn or discolored

• You want to change to the other syringe size

• The syringe leaks or causes air bubbles

• The syringe fails the leak test

Recommendation: Perform the sample syringe leak test whenever you replace the sample syringe (see the ACQUITY UPLC online Help).

Air bubbles in the sample syringe adversely affect system pressure, baseline, volume, and peak area.

Required materials

• Degassed, weak wash solvent


• Replacement sample syringe


Caution: To avoid breaking the sample syringe, do not attempt to remove air bubbles from the syringe by tapping on it.

Warning: To avoid a pinch injury or puncture wound, ensure that no injection is underway or pending before you remove the sample syringe.



To remove the sample syringe:


2. Click Maintain > Replace > Sample syringe > OK.

Result: Doing so invokes a wizard that moves the syringe to the “down” position.


4. Remove the knurled screw that holds the sample syringe to the syringe mounting bracket.

Sample syringe assembly components:

5. Unscrew the sample syringe, counterclockwise, until it separates from the sample syringe valve.


Sample syringe

Knurled screw

Knurled collar



6. Depress the syringe barrel to clear the top mounting bracket, and remove the syringe.

To install a new sample syringe:

1. Carefully unpack the replacement sample syringe.

2. Partially fill the new syringe (by hand) with weak wash solvent (to help remove air bubbles).

Requirement: Make sure all air bubbles are removed.

3. Retract the syringe plunger so that the plunger end slides over the threaded post on the syringe guide mounting bracket.

4. Screw the new sample syringe partially into the sample syringe valve.

5. Finger-tighten the sample syringe.

6. Install and finger-tighten the knurled screw that holds the sample syringe plunger to the mounting bracket.

7. Run the Prime Sample Syringe Only option until no air bubbles remain in the sample syringe.


Caution: To avoid breaking the syringe, do not grasp it by its glass barrel. Always grasp the syringe by its knurled collar.


Mounting bracket

Threaded holder

Syringe plunger

Knurled collar



Cleaning the injection port

Important: To ensure consistent injection performance, clean the injection port weekly or monthly, as necessary.

The surfaces of the injection port can become coated with salt or buffer buildup, especially for systems used in biopharmaceutical applications. The buildup can interfere with the motion of the injection port. The frequency of cleaning varies according to system usage and solvent composition.

Required materials

• T20 TORX driver

• Spray bottle


• Nonabrasive, lint-free wipes

• HPLC-grade water

To clean the injection port:

1. Stop the solvent flow.

2. Open the sample compartment and fluidics compartment doors.

3. Remove the sample plate.


5. Click Maintain > Replace > Seal.

Result: Doing so moves the needle carriage towards the back of the sample compartment, improving access to the injection port.

Warning: To avoid personal contamination with biohazards and toxic compounds, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Warning: To avoid eye injury, wear safety glasses when cleaning the injection port.


Cleaning the injection port

6. Open the SM-FTN’s door.

7. Using the T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel.

8. Dampen a nonabrasive, lint-free wipe with HPLC-grade water and use it to clean the areas of the injection port shaded in the image below.




9. In the ACQUITY UPLC Console, click Maintain > Change Needle.

10. The needle carriage moves to the front of the sample compartment, improving access to the needle.

11. Place a nonabrasive, lint-free wipe underneath the needle carriage.

12. Spray the needle and needle guide with HPLC-grade water to wash off any deposits.

13. Allow the water and deposits to drip off the needle, and then carefully remove the wipe from beneath the needle.

14. Reinstall the access panel using the T20 TORX driver to tighten the two screws that secure the panel to the front of the unit.

Requirement: Ensure that the seal port tube is routed through the gap in the insulation on the side of the access panel.

Needle guide

Needle

Access panel gap


Replacing the injection valve cartridge


16. Reset the SM-FTN.

Replacing the injection valve cartridge

Requirement: When you replace the injection valve, you must also replace the sample loop.

Required materials

• 2-mm Allen wrench


• Injection valve cartridge

To remove the injection valve cartridge:


2. In the SM-FTN information window, click Maintain > Park needle and inject valve > Yes.



Warning: To avoid personal contamination with biohazards and toxic compounds, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Caution: To avoid damaging electrical parts, never disconnect an electrical assembly while power is applied to an instrument or device. To completely interrupt power, set the power switch to Off, and then unplug the power cord from the AC source. Wait 10 seconds thereafter before you disconnect an assembly.



5. Remove the fittings attached to the injection valve cartridge.

6. Use the 2-mm Allen wrench to remove the screw at the 10 o’clock position on the injection valve cartridge.

7. Remove the injection valve cartridge from the injection valve assembly.

To install a new injection valve cartridge:

1. Carefully unpack the replacement injection valve cartridge.

2. Ensure that the groove in the cartridge housing and the groove on the drive clamp align. If they do not align, turn the drive clamp until the grooves line up.

3. Insert the new injection valve cartridge into the injection valve assembly.

Note: If the injection valve cartridge does not slide fully into the injection valve assembly, contact your Waters service representative.

4. Insert the 2-mm Allen screw at the 10 o’clock position on the injection valve cartridge and tighten it.

From injection port

To column


To waste

Sample syringe

Needle

Drive clamp

Aligned grooves

Injection valve cartridge housing


Cleaning the exterior of the SM-FTN

5. Reattach all fittings, ensuring the tubes sit on the bottoms of their fitting holes.



Cleaning the exterior of the SM-FTN

Clean surfaces of the SM-FTN using only a soft, lint-free paper or cloth dampened with water.

Observe these requirements when cleaning device surfaces:

• Always ensure the electrical power to the device is interrupted.

• Always use eye and hand protection during the cleaning process.

• Apply the water to a clean cloth only, and then wipe the device.

• Never spray or apply the water directly onto any device surface.


A Safety Advisories

Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product’s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of the products that Waters offers.

Contents:

Topic Page

Warning symbols.............................................................................. 100

Caution advisory .............................................................................. 103

Warnings that apply to all Waters instruments and devices ........ 104

Warnings that address the replacing of fuses ................................ 109

Electrical and handling symbols ..................................................... 111


A Safety Advisories

Warning symbols

Warning symbols alert you to the risk of death, injury or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it.

Warning: (Risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of sharp-point puncture injury.)

Warning: (Moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)


Warning symbols

Specific warnings

The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts.

Burst warning

This warning applies to Waters instruments and devices fitted with nonmetallic tubing.

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Warning: (Use eye protection.)

Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized:• Wear eye protection.• Extinguish all nearby flames.• Do not use tubing that is, or has been, stressed or kinked.• Do not expose nonmetallic tubing to incompatible compounds like

tetrahydrofuran (THF) and nitric or sulfuric acids.• Be aware that some compounds, like methylene chloride and

dimethyl sulfoxide, can cause nonmetallic tubing to swell, significantly reducing the pressure at which the tubing can rupture.


A Safety Advisories

Biohazard warning

The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans.

Biohazard and chemical hazard warning

This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials.

Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult your the biohazard safety representative for your organization regarding the proper use and handling of infectious substances.

Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials.


Caution advisory

Caution advisory

Caution advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample’s integrity. The exclamation point symbol and its associated statement alert you to such risk.

Caution: To avoid damaging the instrument’s case, do not clean it with abrasives or solvents.


A Safety Advisories

Warnings that apply to all Waters instruments and devices

When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.

注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設

備的權利。

注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操作该设备的合法性。

주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

注意：規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとしての承認が無効になる可能性があります。



Warning: Use caution when working with any polymer tubing under pressure:• Always wear eye protection when near pressurized polymer tubing.• Extinguish all nearby flames.• Do not use tubing that has been severely stressed or kinked.• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated

nitric or sulfuric acids.• Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic

tubing to swell, which greatly reduces the rupture pressure of the tubing.Attention: Manipulez les tubes en polymère sous pression avec precaution:• Portez systématiquement des lunettes de protection lorsque vous vous

trouvez à proximité de tubes en polymère pressurisés.• Eteignez toute flamme se trouvant à proximité de l’instrument.• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF)

ou de l'acide sulfurique ou nitrique concentré.• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le

gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht:• In der Nähe von unter Druck stehenden Polymerschläuchen stets

Schutzbrille tragen.• Alle offenen Flammen in der Nähe löschen.• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind.• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder

konzentrierte Salpeter- oder Schwefelsäure verwenden.• Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische

Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.


A Safety Advisories

Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione:• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero

pressurizzati.• Spegnere tutte le fiamme vive nell'ambiente circostante.• Non utilizzare tubi eccessivamente logorati o piegati.• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico

o nitrico concentrati.• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano

rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi.

Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión:• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos

de polímero sometidos a presión.• Si hubiera alguna llama las proximidades.• No se debe trabajar con tubos que se hayan doblado o sometido a altas

presiones.• Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano

(THF) o ácidos nítrico o sulfúrico concentrados.• Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo

dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.

警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。

• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。

• 熄滅附近所有的火焰。

• 不要使用已經被壓癟或嚴重彎曲管線。

• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。

• 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。



警告：当有压力的情况下使用管线时，小心注意以下几点：

• 当接近有压力的聚合物管线时一定要戴防护眼镜。

• 熄灭附近所有的火焰。

• 不要使用已经被压瘪或严重弯曲的管线。

• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。

• 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。

경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.• 근처의 화기를 모두 끄십시오.• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축 질산 또는 황산과 함께 사용하지 마십시오.

• 염화 메틸렌(Methylene chloride) 및 디메틸술폭시드(Dimethyl sulfoxide)는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오.

警告：圧力のかかったポリマーチューブを扱うときは、注意してください。

• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。

• 近くにある火を消してください。

• 著しく変形した、または折れ曲がったチューブは使用しないでください。

• 非金属チューブには、テトラヒドロフラン(THF)や高濃度の硝酸または硫酸などを流

さないでください。

• 塩化メチレンやジメチルスルホキシドは、非金属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧力で破裂します。


A Safety Advisories

Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。

경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다.

警告：ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。


Warnings that address the replacing of fuses

Warnings that address the replacing of fuses

The following warnings pertain to instruments equipped with user-replaceable fuses.

If the fuse types and ratings appear on the instrument:

Warning: To protect against fire, replace fuses with those of the type and rating printed on panels adjacent to instrument fuse covers.Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument.Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind.Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti alla copertura fusibili dello strumento.Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características impresos en los paneles adyacentes a las cubiertas de los fusibles del instrumento.

警告：為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類型與規格的保險絲。

警告：为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝。

경고: 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오.

警告：火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプおよび定格のヒューズをご使用ください。


A Safety Advisories

If the fuse types and ratings do not appear on the instrument:

Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter.Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des fusibles" du chapitre traitant des procédures de maintenance.Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" des Kapitels "Wartungsverfahren" angegeben sind.Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo "Sostituzione dei fusibili" del capitolo "Procedure di manutenzione".Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características indicados en la sección "Sustituir fusibles".

警告：為了避免火災，更換保險絲時，應使用「維護步驟」章節中「更換保險絲」所指定之相同類型與規格的保險絲。

警告：为了避免火灾，应更换 “维护步骤 ”一章的 “更换保险丝 ”一节中介绍的相同类型和规格的保险丝。

경고: 화재의 위험을 막으려면 유지관리 절차 단원의 “퓨즈 교체” 절에 설명된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오.

警告：火災予防のために、ヒューズ交換ではメンテナンス項目の「ヒューズの交換」に記載されているタイプおよび定格のヒューズをご使用ください。


Electrical and handling symbols

Electrical and handling symbols

Electrical symbols

The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels.

Electrical power on

Electrical power off

Standby

Direct current

Alternating current (single phase)

Protective earth terminal

Frame or chassis ground

Fuse


A Safety Advisories

Handling symbols

The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.

Keep upright!

Keep dry!

Fragile!

Use no hooks!


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