Acta Medica OkayamaVolume 50, Issue 5 1996 Article 3

OCTOBER 1996

Rat Parathyroid Gland, with Special Referenceto Its Blood Vascular Bed, Pericapillary Space

and Intercellular Space

Toshihisa Tanaka∗ Mari Tsubouchi† Yutaka Tsubouchi‡

Aiji Ohtsuka∗∗ Takuro Murakami††

∗Okayama University,†Okayama University,‡Okayama Univeristy,∗∗Okayama University,††Okayama University,

Copyright c©1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.

Rat Parathyroid Gland, with Special Referenceto Its Blood Vascular Bed, Pericapillary Space

and Intercellular Space∗

Toshihisa Tanaka, Mari Tsubouchi, Yutaka Tsubouchi, Aiji Ohtsuka, and TakuroMurakami

Abstract

The blood vascular bed, perivascular space and intercellular space of the rat parathyroid glandwere studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples,and NaOH-digested tissue blocks. The findings were supplemented by transmission light and elec-tron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroidgland contained a rich network of capillaries. These capillaries were surrounded by marked peri-capillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells.The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projec-tions which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymalcells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cellsshowed strong negative charging, and maintained the intercellular spaces. The luminal surfacesof the capillary endothelium also showed strong negative charging, and maintained the capillarylumen.

KEYWORDS: parathyroid gland, cationic and anionic iron colloid stainings, vascular casting,freeze fracture, maceration

∗PMID: 8914677 [PubMed - indexed for MEDLINE]Copyright (C) OKAYAMA UNIVERSITY MEDICAL SCHOOL

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

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Acta Medica Okayama, Vol. 50 [1996], Iss. 5, Art. 3

http://escholarship.lib.okayama-u.ac.jp/amo/vol50/iss5/3

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

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Acta Medica Okayama, Vol. 50 [1996], Iss. 5, Art. 3

http://escholarship.lib.okayama-u.ac.jp/amo/vol50/iss5/3

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

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Acta Medica Okayama, Vol. 50 [1996], Iss. 5, Art. 3

http://escholarship.lib.okayama-u.ac.jp/amo/vol50/iss5/3

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

Produced by The Berkeley Electronic Press, 1996

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Acta Medica Okayama, Vol. 50 [1996], Iss. 5, Art. 3

http://escholarship.lib.okayama-u.ac.jp/amo/vol50/iss5/3

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

Produced by The Berkeley Electronic Press, 1996

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Acta Medica Okayama, Vol. 50 [1996], Iss. 5, Art. 3

http://escholarship.lib.okayama-u.ac.jp/amo/vol50/iss5/3

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Tanaka et al.: Rat Parathyroid Gland, with Special Reference to Its Blood

Produced by The Berkeley Electronic Press, 1996