activities on sweet cherry genetic resources at cra-frf ... · activities on sweet cherry genetic...
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Activities on sweet cherry genetic
resources at CRA-FRF and DEMETRA
Daniela Giovannini
CRA-FRF, Unità di Ricerca per la
Frutticoltura di Forlì
Annalisa Marchese
Dipartimento DEMETRA
Università di Palermo
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
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Italy displays many, heterogeneous climatic conditions
hundreds of local varieties
progressive loss of traditional varieties
links with the territory
source of useful traits for breeding
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
Sweet cherry in Italy has been cultivated for more than 2000 years
Initiatives, at the local level, to preserve heritage varieties
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Corniola
Morphological & phenological characterization of the collected germplasm
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Fiore
Duroncino di Cesena
Morphological & phenological characterization of the collected germplasm
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Don Antoni
Cappuccia di Chiusa Sclafani
Morphological & phenological characterization of the collected germplasm
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Kronio
Morphological & phenological characterization of the collected germplasm
Naturally self-compatible (Marchese et al., 2007)
S5’S6, where S5’ derives from a mutation in the S5 pollen component
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Morphological & phenological characterization of the collected germplasm
Bombardune
Carrammendula 1
Zuccaredda
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Caddusa Moscatella C. Sclafani
Cappuccia Castelbuono Nucigliera
Cappuccia Imperiale Niredda Cappuccia
Carrubbedda Nireddi
Cristallina Puntalazzese
Furticchiara Quarantana
Itala S.Mela Raffiuna S.Mela
Kronio
Maiolina S.Mela
Sant’Angelo S.Mela
San Pitrisa Zappia
Corniola A Durone di Cesena B
Corniola B Durone Nero I
Corniola C Durone Nero II
Durella A Fiore A
Durella B Fiore B
Durona di S.Giovanni Gemella
Duroncino di Cesena Morandina
Durone Compatto di Vignola Morena A
Durone di Cesena A Morena B
Accessions studied & their territory of origin
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
Abenavoli bianco Citra Napoletana
Abenavoli nero Cuore Aspromonte Niredda
Amarena grecanica Cuore Serre Piede corto
Bombardune Grammendula 1 San Pietro 1
Cannamele Grammendula 2 San Pietro 2
Cappucciarica 1 Iancuzza napoletana Santa Nutrice
Cappucciarica 2 Lombardune Semenzale durone
Carraffune Maiatica Semenzale morbido
Carraffune bianco Maiatica rossa Vallescura 1
Carraffune biondo Maiolina Piana Vallescura 2
Carraffune nero Maiolina Serre Zuccaredda
Carrammendula A Moddacchia 1 Zuccarigna
Carrammendula B Moddacchia2
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(Tobutt & Clarke, 2009)
Standard set of primers
& Reference cvs.
Genetic diversity assessment
64 accessions
5 Reference cvs.
59 Italian accessions
16 from E.Romagna
14 from Sicilia
29 from Calabria
References
(source EMR)
Noble
Noir de Meched
Napoleon
Goodnestone Black
Colney
Primer Rank
CPPCT006 1
CPPCT22 2
EMPA017 3
EMPA002 4
EMPaS10 5
EMPaS12 6
EMPaS01 7
BPPCT037 8
PceGA34 9
EMPaS06 10
EMPaS02 11
UDP98-412 12
EMPaS14 13
EMPA026 14
PS05C03 15
EMPA003 16
10 out of 16 SSRs used
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
http://www.bordeaux.inra.fr/euprunusdb/
EPDB Portal
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0.1
(1) Durone di Cesena I (4) Noir de Meched (3) Cristallina (2) Cuore Aspromonte (2) Vallescura II (2) Maiatica (2) Vallescura I (4) Goodnestone Black (4) Colney (3) Quarantana (1) Durella II (3) Kronio (1) Duroncino (1) Durone Nero I (4) Noble (1) Durone Compatto Vignola (1) Durone di Cesena II (1) Durone Nero II (3) San Pitrisa Zappia (2) Grammendula II (1) Durona Sangiovanni (1) Cornetta I (1) Cornetta II (1) Fiore I (1) Fiore II (1) Durella I (1) Morandina (2) Amarena grecanica (1) Morena (3) Cappuccia Castelbuono (3) Carrubbedda (2) San Pietro San Lorenzo (2) Zuccaredda (3) Caddusa (3) Itala S. Mela (2) Maiatica rossa (3) Moscatella Chiusa Scalfani (3) Maiolina S. Mela (2) Piede Corto (3) Nireddi (3) Puntalazzese (2) Carraffune bianco (2) Lombardune (3) Niredda Cappuccia (2) Semenzale Morbido (1) Gemella (3) Furticchiara (4) Napoleon (2) Grammendula I (3) Cappuccia Imperiale (2) Iancuzza Napoletana (2) Carraffune (3) Raffiuna S. Mela (2) Abenavoli bianco (2) Zuccarigna (2) Bombardune (2) Carrammendula II (2) Moddacchia Aspromonte (3) Nucigliera (3) Sant'Angelo S.Mela (2) Citra (2) San Pietro Cardeto (2) Cappucciarica (2) Cappucciarica San Lorenzo
54 distinct profiles
17 non-distinguishable profiles
2 to 15 alleles/SSR locus (mean = 7.6);
He range 0.37-1.00 (mean = 0.77);
PIC range 0.38-0.75 (mean= 0.60);
Most polymorphic SSRs in the set:
BPPCT037 (15) and PceGA34 (12)
Least polymorphic:
EMPA002 (2), EMPA003 (4) and EMPA017 (4)
Genetic diversity assessment
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
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45 Italian accessions (37 unique genotypes)
Cvs. of known S-genotype
amplification of S-RNase and SFB genes
0,00
0,03
0,06
0,09
0,12
0,15
0,18
Fre
qu
en
cy
13 S-alleles detected
17 groups represented
5 ‘new’ groups
2 universal donors
1 self-compatible (S5’)
(In)compatibility group assignment*
S3, S6 , S13 and S16 are well represented across Italian germplasm;
S1, S2 are rare;
S4 is absent (the only accession carrying S4 is most probably a synonym of Napoleon);
*according to Tobutt et al. (2004); Marchese et al. (2007), Schuster et al. (2007), Ipek et al. (2011).
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
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Future breeding goals
Self-compatibility
Environmental adaptability
low chilling requirement
Extended ripening season
very early, late
Better fruit quality
good taste
high flesh firmness
high sugar content
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
Availability of diverse germplasm as a source of useful traits
Kronio self-progeny
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People
Thanks for your attention
WG1 : Application of genomic approaches to breeding cherries. Joint meeting with the 6th Rosaceae Genome Conference
Daniela Giovannini
Annalisa Leone
Sandro Sirri
Alessandro Liverani
Franco Marra
Annalisa Marchese
Antonino Raimondo
Tiziano Caruso
Rocco Mafrica
Sonia Pangallo
Paolo Pellegrino