aerobic gram negative cocci
DESCRIPTION
Aerobic Gram Negative Cocci. Student Lab Division of Medical Technology Jeanne Filbey, MT(ASCP). Aerobic GNDC. General Characteristics. Normal flora of upper respiratory, alimentary and genitourinary tracts Gram stain: GNDC Oxidase positive Catalase positive Non-motile. Fastidious - PowerPoint PPT PresentationTRANSCRIPT
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Aerobic Gram Negative Cocci
Student LabDivision of Medical Technology
Jeanne Filbey, MT(ASCP)
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General Characteristics• Normal flora of upper respiratory,
alimentary and genitourinary tracts • Gram stain: GNDC• Oxidase positive• Catalase positive• Non-motile
Aerobic GNDC
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Neisseria gonorrhoeae• Fastidious• Temperature sensitive• Culture immediately• Transport media if delay
Aerobic GNDC
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Specimen Gram Stain• Intracellular GNDC
Neisseria gonorrhoeae
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Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth– Contains antibiotics
Neisseria gonorrhoeae
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Identification• Carbohydrates
– Glucose pos– Maltose neg– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria gonorrhoeae
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Pathogenesis• Port of entry• Progression of infection• Human to human transfer• Females asymptomatic
Neisseria gonorrhoeae
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Gonorrhea• Male
– Acute urethritis, prostatitis and epididymitis if untreated
• Female– May be asymptomatic– Cervicitis --> pelvic inflammatory disease --
> scarring of fallopian tubes --> sterility, ectopic pregnancies
Neisseria gonorrhoeae
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Other Infections• Disseminated gonococcal infection• Pharyngitis• Gonococcal ophthalmia neonatorum• Vulvovaginitis
Neisseria gonorrhoeae
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Treatment• Antimicrobial testing
Neisseria gonorrhoeae
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Neisseria meningitidis• Fastidious• Temperature sensitive• Culture immediately• Occasionally grows on SBA
Aerobic GNDC
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Specimen Gram Stain• GNDC
Neisseria meningitidis
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Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth
Neisseria meningitidis
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Identification• Carbohydrates
– Glucose pos– Maltose pos– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria meningitidis
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Pathogenesis• Meningitis• Septicemia• Septic arthritis• Endocarditis• Pneumonia• Urethritis• Asymptomatic nasopharyngeal carriers
Neisseria meningitidis
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Neisseria lactamica• Normal flora
Aerobic GNDC
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Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth
Neisseria lactamica
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Identification• Carbohydrates
– Glucose pos– Maltose pos– Lactose pos– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria lactamica
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Moraxella (Branhamella) catarrhalis• Can be normal respiratory flora
Aerobic GNDC
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Colony Morphology• Sheep blood agar• Chocolate agar• Thayer-Martin
agar– Growth
Moraxella (Branhamella) catarrhalis
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Identification• Carbohydrates
– Glucose neg– Maltose neg– Lactose neg– Sucrose neg
• DNase pos• Nitrate pos• Butyrate esterase pos
Moraxella (Branhamella) catarrhalis
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Pathogenesis• Acute bronchitis• Pneumonia• Meningitis• Seen in immunocompromised patients
(elderly)• Otitis media & maxillary sinusitis in kids• Most produce beta lactamase
Moraxella (Branhamella) catarrhalis
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Summary• Specimen handling and processing• Growth characteristics• Identification• Clinical significance• Antimicrobial testing
Aerobic GNDC
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Who am I?
Urethral gram stain Thayer Martin agar
Neisseria gonorrhoeae
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Who am I?
CSF gram stain Chocolate agar
Neisseria meningitidis