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OIE Collaborating Centre Reports

Activities in 2013

Title of Collaborating Centre: Diagnosis of Animal Diseases and Vaccine Evaluation in the Americas

Address of Collaborating Centre: National Veterinary Services Laboratories and Center for Veterinary Biologics, 1920 Dayton Avenue,

Ames, Iowa 50010

Tel.: +1-515-337-6100; +1-515-337-7266

Fax: +1-515-337-6316; +1-515-337-7397

e-mail address: [email protected]; [email protected]

website: http://www.aphis.usda.gov/animal_health/lab_info_serviceshttp://www.aphis.usda.gov/animal_health/vet_biologics/

Name of Director of Institute (Responsible Official):

Steven A. Karli, Acting DirectorCenter for Veterinary Biologics, STAS, VS, APHIS,

USDA

Dr. Paul J. HauerAssociate Director, National Veterinary Services

Laboratories, STAS, VS, APHIS, USDA

Name (including Title and Position) of Head of the

Collaborating Centre (formally OIE Contact Point):

Steven A. Karli, Acting DirectorCenter for Veterinary Biologics, VS, APHIS, USDA

Dr. Paul J. HauerAssociate Director, National Veterinary Services

Laboratories, STAS, VS, APHIS, USDA

Name (including Title and Position) of writer of this report

(if different from above)

Dr. Beth HarrisChief of Staff, National Veterinary Services

Laboratories, STAS, VS, APHIS, [email protected]

Ms. Chelsea J. Bare, Staff Assistant, VS, APHIS, USDA

[email protected]

Annual reports of OIE Reference Centres, 2013 1

Diagnosis of Animal Diseases and Vaccine Evaluation in the Americas

Summary of activities specifically related to the mandate of OIE Collaborating Centres

ToR: To provide services to the OIE, in particular within the region, in the designated specialty, in support of the implementation of OIE policies and, where required, seek for collaboration with OIE Reference Laboratories

ToR: To identify and maintain existing expertise, in particular within its region

1. Activities as a centre of research, expertise, standardisation and dissemination of techniques within the remit of the mandate given by the OIE

Disease control Epidemiology, surveillance, risk assessment, modelling Training, capacity building Zoonoses Wildlife Avian diseases Aquatic animal diseases Animal welfare Diagnosis, biotechnology and laboratory Veterinary medicinal products Vaccines Food safety Feed safety Other (Name the category: )

DIAGNOSIS, BIOTECHNOLOGY AND LABORATORY

Title of activity Scope

Diagnostic activities for Aujesky’s Disease [Pseudorabies (PRV)]

Testing for Ajueszky’s Disease was completed on 553 samples originating within the United States (US) using gB ELISA, gI ELISA, Autolex and Virus Neutralization (VN), and 41 samples of international origin using the same test methods. Virus Isolation (VI) and PCR testing was completed on an additional 20 samples from the US and 41 international samples.The NVSL also produced the following reagents and proficiency test panels which were supplied to OIE Member Countries: PRV virus and positive control sera for VN, antisera for various serology tests, fluorescent antibody (FA) conjugate, and proficiency panels for latex agglutination, gB ELISA, gI ELISA, and VN test methods.

Diagnostic activities for Avian Influenza Virus (AIV) and Newcastle Disease (ND)

Diagnostic testing for AIV and ND was completed on 31,310 samples of national origin using the indirect test methods of agar gel immunodiffusion (AGID) Hemagglutination (HI) and neuraminidase-inhibition (NI) antibody subtype identification for AIV, and HI antibody identification for avian paramyxovirus (APMV). Another 247 samples of international origin were tested by AGID for AIV. NVSL tested another 27,807 diagnostic samples of domestic origin using the direct test methods of HI and NI virus subtype identification, real-time RT-PCR for IAV, ND and APMV; and virus isolation (VI), genomic sequencing and pathogenicity testing for AIV and APMV. A total of 78 samples of international origin were also tested using these techniques.Reagents produced and provided to OIE Member countries included; positive control antigens and type antisera for AIV HI testing; positive and negative control sera and antigen for AGID testing, IAV matrix gene and APMV-1 matrix gene positive amplification and extraction controls for real-time PCR,

2 Annual reports of OIE Reference Centres, 2013


H5 and H7 gene real-time PCR positive amplification controls, APMV-1 control positive antigen and positive antisera for HI testing.Two proficiency tests were also produced; a real-time RT PCR panel and an AGID proficiency test.NVSL also provided diagnostic support for three OIE Member countries, and tested a total of 221 samples for routine diagnostic testing, and another 9 samples for confirmatory diagnosis.

Diagnostic activities for Anthrax

A total of 9 samples were submitted to the NVSL for bacteriological culture in 2013, with all samples originating within the U.S.Gamma phage was also produced and distributed to OIE Member Countries for use in the gamma phage lysis diagnostic test method.

Diagnostic activities for bluetongue virus (BTV)

In the US, 697 diagnostic specimens were tested for BTV via indirect test methods [competitive ELISA, AGID, complement fixation (CF) and VN], and another 835 samples were screened using real-time reverse-transcriptase PCR (real-time RT-PCR), nested RT-PCR, and VI.Another 102 international samples were screened by the above direct methods and 232 international samples were screened using real-time RT-PCR, nested RT-PCR, VI, and sheep inoculation.Reagents produced were strong and weak positive reference sera; fluorescent antibody conjugate, Bluetongue virus antiserum for VN, ELISA and AGID ( serotype specific), multiple serotypes of bluetongue stock virus for VN assays, various serotypes for BTV RNA for PCR testing, and a BTV proficiency panel consisting of 20 serum samples for AGID and ELISA methods.Diagnostic testing was carried out for two OIE Member Countries on a total of 27 samples.

Diagnostic activities for contagious equine metritis (CEM)

In 2013 the US tested a total of 1588 samples of domestic origin and 551 samples of international origin using the CF indirect diagnostic test. Another 1167 tests were performed on domestic samples and 1139 tests performed on international samples using the direct methods of culture and identification, identity confirmation and pulse field gel electrophoresis (PFGE).Also in 2013 NVSL produced and provided the following reagents to OIE Member Countries: growth media for CEM (Eugon Agar and other selective agars), CEM high titer control sera for CF testing, CEM control strains for use in identification protocols, a culture and identification proficiency panel, and a CF test ring trial panel.NVSL provided diagnostic consulting to two OIE Member Countries in the form of consultation on the PFGE test.

Diagnostic activities for Eastern equine encephalomyelitis (EEE)

Within the United States (US), a total of 3096 indirect diagnostic tests [CF, hemagglutination inhibition (HI), Plaque Reduction Neutralization (PRN) and IgM Capture ELISA] were performed for EEE, and 66 diagnostic tests performed using RT-PCR. 124 indirect diagnostic tests and 3 direct diagnostic tests were performed on international diagnostic samples using the same methods.NVSL provided diagnostic assistance for five separate OIE Member countries, testing a total of 17 samples submitted during 2013.Reagents supplied to recipient OIE Member countries included EEE virus antigen and antiserum, and normal cell culture antigen for CF, HI and ELISA. EEE equine IgM antiserum for the IgM Capture ELISA test method was also provided.

Diagnostic activities for equine infectious anaemia (EIA)

3,135 diagnostic tests for EIA were performed nationally during 2013, using AGID, ELISA and Immunoblotting. Another 9,382 diagnostic tests were performed for international clients.



Reagents produced include: strong positive, weak positive and negative reference sera for AGID, ELISA and competitive ELISA (cELISA); and an EIA proficiency panel consisting of 20 serum samples for AGID, ELISA and cELISA.Diagnostic testing for diagnostic support was conducted on 170 additional samples for four different OIE Member Countries.

Diagnostic activities for foot and mouth disease (FMD)

A total of 352 indirect tests [ELISA, agar gel immunodiffusion (AGID)] and 923 direct tests [cell cultures, real-time RT PCRs] were performed for diagnostic purposes for foot and mouth disease (FMD) virus on samples originating within the U.S. An additional 18 diagnostic samples of international origin were screened for the detection of FMD using the previously mentioned indirect tests, and 130 international samples were tested using the direct methods listed.Diagnostic testing for diagnostic support was conducted on 9 additional samples for one OIE Member Country.NVSL produced, distributed and evaluated a proficiency test for FMD to 43 participant laboratories in the U.S. Additionally, reagents FMD PCR tests were supplied to 1 OIE Member Country.

Diagnostic activities for Leptospira

In 2013, the NVSL performed 5,867 tests [Leptospira microscopic agglutination test (MAT)] for detection of Leptospira sp. on samples from the US, and another 150 tests on international samples.The following diagnostic reagents were produced and supplied to other OIE Member Countries; reference positive and negative control sera for MAT, reference cultures for MAT, multivalent fluorescent antibody conjugate, and Leptospira culture media.

Diagnostic activities for Swine influenza virus (SIV)

A total of 82 HI serology tests were performed on samples originating outside of the US, and another 319 samples originating in the U.S. were tested by direct methods [RT-PCR, VI and sequencing].The NVSL produced and provided the following products to support OIE diagnostic testing activities in Member Countries: SIV H1N1 virus, antigen and antiserum; H3N2 virus, antigen, and antiserum; FA conjugate; PCR positive controls; PCR proficiency panels; and field isolates from surveillance testing.

Diagnostic activities for Venezuelan equine encephalomyelitis (VEE)

A total of 521 diagnostic samples originating within the US were tested by indirect methods for VEE, using the same tests described above for EEE, and another 103 diagnostic samples of international origin were tested. Three direct diagnostic tests were performed on samples of international origin using RT-PCR.Diagnostic reagents for VEE that were produced and supplied to recipient OIE Member Countries in 2013 were VEE virus antigen and antiserum for performing the HI, DF, ELISA and PRN test methods.Five OIE Member Countries requested assistance with diagnostic support for VEE, and the NVSL tested 19 samples as part of this support.

Diagnostic activities for Vesicular Stomatitis Virus (VSV)

5,854 samples of national origin were tested for VSV using indirect methods of cELISA, CF and VN in2013; another 558 samples of international origin were tested for diagnostic purposes using the same methods. Another 1016 samples of national origin were tested for diagnostic purposes using the direct diagnostic methods of VI, PCR and antigen capture ELISA.The NVSL also produced reagents for VN and CF that were distributed to several OIE Member Countries. Reagents include complement, modifying factor, normal mouse brain antigen, VSV antigens, and positive and negative control sera for NJ and IN strains of VSV, plus positive and negative control sera and viruses for both NJ and IN VSV VN test methods. A total of 15 proficiency panels for the VSV-CF serology test and 2 proficiency panels for VSV-VN test were provided to laboratories within the U.S, and 2 additional



VSV-VN test panels provided to 2 OIE Member Countries. No proficiency panels were produced in 2013.One OIE Member Country also requested assistance in the form of diagnostic testing of 9 samples for VSV.

Diagnostic activities for Western equine encephalomyelitis (WEE)

In 2013, NVSL tested 2030 diagnostic samples from the US via the indirect methods of CF, HI and PRN, and another 70 samples of international origin using the same assays. A total of 44 diagnostic samples were tested using the direct diagnostic method of RT-PCR from the US, and 3 international samples.WEE virus antigen and antiserum reagents were produced and provided to OIE Member Countries for use in HI, CF or ELISA testing.Five OIE Member Countries requested assistance with diagnostic support for WEE, and the NVSL tested 17 samples as part of this support.

Diagnostic activities for West Nile Virus (WNV)

During 2013, the NVSL performed 2041 indirect diagnostic tests [equine IgM capture ELISA, PRN, VN] on samples of domestic origin and another 624 indirect tests for samples originating from other countries. For direct diagnostic tests, another 61 RT-PCR tests were performed on domestic samples, and 4 RT-PCR tests done on international samples.NVSL produced the following reagents that were also supplied to OIE Member Countries: WNV RNA for PCR controls, WNV negative, equine IgM low positive, and equine capture IgM antisera for IgM capture ELISAs, antisera for the PRN and VN assays, and an equine IgM ELISA proficiency panel consisting of 10 serum samples/panel.NVSL provided diagnostic assistance to six OIE Member Countries and tested a total of 47 samples for WNV.

VACCINES

Title of activity Scope

VICH Steering Committee Meeting

February 19-21, attended the 28th International Cooperation on Harmonisation of Technical Requirements for Registration of Veterinary Medicinal Products (VICH) Steering Committee, in Washington, DC, as the U.S. delegation representing the USDA on issues related to veterinary biologics.

Visit with Delegates from the People’s Republic of China

On March 21, representatives from NVSL and CVB met with visitors from the People’s Republic of China, as part of a visit coordinated by Iowa’s International Center, International Visitor Leadership Program. The purpose of the visit is to learn more about NVSL and CVB program missions, and the support they offer the OIE.

Facilitated Transfer of Donated Classical Swine Fever Virus

The CVB participated with the National Veterinary Stockpile to facilitate the movement of Classical Swine Fever Vaccine from Germany, through Miami International Airport, to Guatemala for use in their control program.

Visit with Delegates from Indonesia’s Agency of Drug and Food Control

On May 6, several representatives from the Indonesian National Agency of Drug and Food Control met with delegates from the CVB and NVSL to discuss the roles of U.S. Federal agencies in food and product safety regulation and supervision, as well as aspects of risk management, mitigation, evaluation, resolution and communication.

National Vaccine Advisory Committee attendance

Attended several meetings of the National Vaccine Advisory Committee in Washington, DC. The committee’s function is to encourage the availability of adequate supplies of safe and effective vaccination products, recommend research priorities, identify areas of government and non-government cooperation, provide direction to prevent adverse reactions to vaccines, and recommend measures to achieve optimal prevention of human infectious disease through vaccine development.



American Veterinary Medical Association Council on Biologics and Therapeutic Agents

Attended both semi-annual meetings of the council, to discuss and provide information on current and emerging biologics issues.

ToR: To propose or develop methods and procedures that facilitate harmonisation of international standards and guidelines applicable to the designated specialty

2. Proposal or development of any procedure that will facilitate harmonisation of international regulations applicable to the surveillance and control of animal diseases, food safety or animal welfare

Proposal title Scope/Content Applicable area

Validation of In Vitro Alternatives

Attended workshop on Validation of In Vitro Alternatives for In-Process Control of Veterinary Clostridial Vaccines, to determine details of collaborative study to validate cell-based assays as alternatives to the currently used in vivo assays for C. septicum toxins and toxoids.

Surveillance and control of animal diseases Food safety Animal welfare

Animal Influenza Viruses Gap Analysis and Countermeasure Workshop

Attended the National Veterinary Stockpile Animal Influenza Viruses Gap Analysis and Countermeasures Assessment Workshop in Athens, GA, USA. An international group of experts on animal influenza virus met to conduct a gap analysis and assess countermeasures to effectively control and mitigate the impact of an outbreak of an animal influenza virus with zoonotic and/or pandemic potential.


Pharmacovigilance Program Implementation

Attended pharmacovigilance workshop with the Animal Health Institute and the U.S. Food and Drug Administration, Center for Veterinary Medicine, to discuss data evaluation and signal detection association with pharmacovigilance data analysis.


Pharmacovigilance Program Implementation

Met with representatives of the U.S. pharmaceutical and biological industry, as well as U.S. regulatory authorities, to learn about the management of adverse event reporting using the pharmacovigilance software application, PV-Works.


Foreign Animal Disease Gap Analysis and

Attended workshop on Evaluation and Gap Analysis for Border Protection for Foreign Animal

Surveillance and control of animal diseases Food safety



Countermeasure Workshop

Diseases. The workshop addressed issues of improving measures to protect U.S. agriculture from accidental or intentional introduction of foreign animal diseases.

Animal welfare

Intra-governmental Advisory Committee for Strategic Steering (IGACSS)

Attended the National Veterinary Stockpile IGACSS meeting in Riverdale, Maryland. The purpose of the IGACSS meeting is to identify progress made towards the preparedness in combating a foreign animal disease outbreak in the U.S and facilitate future developments.


Optimization and Validation of a

Real-time RT-PCR Assay for Rapid

Detection of Foot-and-Mouth Disease Virus in Bulk Tank

Milk

Development of a sensitive and specific test suitable for passive or active surveillance of bulk tank milk samples.


Modification of H5 real-time RT-PCR to detect Mexican lineage of H5 avian Influenza

Developed new primers and probes for an H5 subtyping assay to detect the Mexican lineages of H5 avian influenza A virus.


Evaluation of commercial swine influenza virus RNA test kit for licensure in avian species.

The VetMax-Gold SIV Detection Kit targets Influenza A viruses, but is licensed only for swine species in the U.S. This study is evaluating its use in avian species.


Improved diagnostics for the detection of H7 viruses

Testing for updated Eurasian-specific H7 assay


ToR: To establish and maintain a network with other OIE Collaborating Centres designated for the same specialty, and should the need arise, with Collaborating Centres in other disciplines

ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other centres, laboratories or organisations

3. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres (CC), Reference Laboratories (RL), or organisations designated for the same specialty, to coordinate scientific and technical studies?

Yes No


http://www.oie.int/en/our-scientific-expertise/collaborating-centres/reference-centre-networks/


Name of OIE CC/RL/other organisation(s) Location

Region of networking

CentrePurpose

Pirbright Institute;European Union Reference Laboratories (EURL), FAO

World Reference Laboratory (WRL)

UK Africa Americas Asia and Pacific Europe Middle East

Optimization and validation of a real-time RT-PCR assay for rapid detection

of FMD bulk tank milk samples. Development and validation of an RNA extraction method and multiplex RT-qPCR for detection of FMDV in bulk

tank milk suitable for surveillance in the dairy industry.

USDA Agricultural Research Services (ARS) South East Poultry Research Laboratory (SEPRL); USDA APHIS National Wildlife Disease Program

Athens, GA, USA; Fort

Collins, CO, USA

Africa Americas Asia and Pacific Europe Middle East

Collaboration on Newcastle disease surveillance program. Samples that were test positive for Influenza A or

Newcastle disease were submitted to NVSL for PCR and virus isolation.

SEPRL, Friedrich-Loeffler-Institut (FLI)

Athens, GA, USA;

Germany


As part of a One Health Diagnostic Collaboration on avian influenza

A(H7N9) between USDA & the Centers for Disease Control and Prevention

(CDC), a human-origin avian influenza A(H7N9) virus [A/Anhui/1/2013] was

shared with CDC from China, and subsequently shared with animal health

partners at the NVSL and SEPRL. The virus was propagated and pathotyped

and in a cooperative effort working with national partners such as the

SEPRL as well as international influenza reference laboratories an updated

Eurasian-specific H7 assay was developed.

4. Did your Collaborating Centre maintain a network with other OIE Collaborating Centres, Reference laboratories, or organisations in other disciplines, to coordinate scientific and technical studies?

Yes No

Name of OIE CC/RL/other organisation(s) Location

Region of networking

CentrePurpose

Pan American Centre for Foot-and-Mouth

Disease/Pan American Health Organization

(PANAFTOSA)

Rio de Janeiro,

Brazil


Evaluation of FMD 01 Campos vaccine protection against the FMD 01 Ecuador.

Australian National Quality Assurance Program

Australia Africa Americas Asia and Pacific Europe Middle East

Participation in quality system accreditation Leptospira microscopic agglutination test

proficiency test.



Pirbright Institute (AHVLA), Bury St., Edmonds

Pirbright, UK


Participation in inter-laboratory test comparison for contagious equine metritis.

AHVLA

European Union Reference Laboratories (EURL), FAO

World Reference Laboratory (WRL)

Pirbright, UK


Participation in the annual foot-and-mouth disease (FMD) and swine vesicular disease (SVD) combined proficiency test scheme

(PTS).

Canadian Food Inspection Agency (CFIA), AHVLA,

Australian Animal Health Laboratory (AAHL), NVSL,

Istitutio Zooprofilattico Sperimentale delle Venezie (IZSV), Hokkaido University-

Japan, Indian Veterinary Research Institute (IVRI), FLI,

SEPRL

Canada, England, Australia, USA, Italy,

Japan,India,

Germany


Avian influenza ring trial for molecular detection and pathotyping of H5 and H7

subtypes of avian influenza.

CFIA Winnipeg, Canada


Harmonization of diagnostic tests for the detection of avian influenza virus and the evaluation of a new rRT-PCR assay for the

detection of H5 AIV.

CFIA Winnipeg, Canada


Proficiency testing for avian influenza AGID test, harmonization of antibody detection

ToR: To place expert consultants at the disposal of the OIE.

5. Did your Collaborating Centre place expert consultants at the disposal of the OIE?

Yes No

Name of expert Kind of consultancy Subject

Multiple NVSL and CVB representatives

Review of chapters Review and comment on chapters in OIE Manual of Standards for

Diagnostic Tests and Vaccines for Terrestrial Animals.

Mr. Steve Karli Official USDA delegate for the Committee of the Americas for the Harmonization of Registration and

Control of Veterinary Medicines (CAMEVET) in Panama City, Panama,

September 24-28.

Information on the USDA biologics program activities.

Dr. Byron Rippke Attended the 29th Meeting of the VICH Steering Committee in Auckland, New Zealand, as part of the U.S. delegation

representing the USDA on issues related to veterinary biologics.

VICH steering committee issues regarding veterinary biologics.



Name of expert Kind of consultancy Subject

Dr. Sabrina Swenson Subject matter expertise Swine Influenza Virus (SIV) diagnostic test reagent and proficiency test

availability; technical assistance with reagents for hemagglutination

inhibition testing for SIV; Vesicular stomatitis virus (VSV) diagnostic test

reagent availability, technical assistance on diagnostic methods for

VSV.

Dr. Bev Schmitt Member of the OIE Biological Standards Commission

Review of matters pertaining to diagnosis, recognition, epidemiology and control measures of diseases at

OIE Reference Laboratories and Collaboration Centres.

Dr. Bev Schmitt Member of the FAO-OIE Rinderpest Joint Advisory Committee

Committee overviews Rinderpest post-eradication activities, including

destruction and sequestration of Rinderpest virus stocks and the review

and approval of laboratories designated to serve as future holding

facilities.

Dr. Matt Erdman Subject matter expertise Consultation on contagious equine metritis molecular epidemiology

techniques.

Ms. Ginger Harvey Subject matter expertise Provided expert advice and standard operating procedures on biosafety

level 3 (BSL3) laboratory facilities for anthrax.

Dr. Consuelo Carrillo Technical training and consultancy Foot-and-mouth disease diagnostic protocols and test methodology, analysis of epidemiological data.

Ms. Jan Pedersen OIE twinning project with Chile Provided expert advice on biosafety level 2 (BSL2) and biosafety level 3

(BSL3) laboratory and animal facilities, conduction of the Avian Influenza (AI)

AGID test, and AI and Newcastle disease nucleotide sequencing.

Ms. Jan Pedersen Training Training on avian influenza virus (AIV) and Newcastle disease virus (NDV) validation procedures and methods

and data evaluation for the validation of rRT-PCR assays Training event held at the NVSL in Ames, IA, USA on Jun 3-

7, 2013.

Dr. Mia Torchetti Subject matter expertise Expertise in monitoring, laboratory policy, global/regional networking,

and novel influenza viruses.

Dr. Nichole Hines Training Training for AIV and NDV validation procedures and methods; held at the

LAVECEN, Dominican Republic, August 26-30, 2013.



ToR: To provide, within the designated specialty, scientific and technical training to personnel from OIE Member Countries

6. Did your Collaborating Centre provide scientific and technical training, within the remit of the mandate given by the OIE, to personnel from OIE Member Countries?

Yes No

If the answer is yes, please provide the total number of trained persons for each of the following categories:

a) Technical visits:................................................................63

b) Seminars:.........................................................................24

c) Hands-on training courses:..............................................81

d) Internships (>1 month):...................................................

Type of technical training provided

(a, b, c or d)Content

Country of origin of the expert(s) provided with

training

No. participants from the corresponding

country

a Exchange of technical information on veterinary biologics licensing

Japan 3

a Participated on technical working group to develop diagnostic and

control strategies for the U.S. and Canada for Pseudorabies, Rift Valley

Fever, and Classical Swine Fever

Canada ~ 50

c Institute for International Cooperation in Animal Biologics (IICAB), OIE Veterinary Biologics Training Program (also listed in

item 7)

16 different countries of origin

32

a Technical training provided on protocols for diagnostic sequencing

of FMD isolates

Kazakhstan 10

b Seminars covering technical aspects of FMD diagnostic test methods

Kazakhstan 24

c Hand’s on training in diagnostic techniques for viral diseases, emphasizing foot-and-mouth

disease

Kazakhstan 7

c Foreign Animal Disease Diagnostic Training courses

16 different countries of origin

35

c Training for AIV and NDV validation procedures and methods

Dominican Republic 6

c Training on avian influenza virus (AIV) and Newcastle disease virus (NDV) validation procedures and methods and data evaluation for the validation of rRT-PCR assays

Brazil 1



ToR: To organise and participate in scientific meetings and other activities on behalf of the OIE

7. Did your Collaborating Centre organise or participate in the organisation of scientific meetings on behalf of the OIE?

Yes No

National/International Title of event Co-organiser Date

(mm/yy) Location No. Participants

International Institute for International

Cooperation in Animal Biologics (IICAB), OIE Veterinary Biologics

Training Program

IICAB, OIE May 2013 Iowa, U.S. 127, including 32 international

from 16 different countries

International COSALFA 40 Meetings COSALFA April 15- 19, 2013

Panama City,

Panama

unknown

International OFFLU Swine Influenza Virus Group Meeting

OIE, FAO April 16-17, 2013

Rome, Italy 25

International International FMD Vaccine Strategic

Reserves Conference

OIE, FAO October, 2013

N/A(tele-

conference)

11

International Joint ECDC/WHO European Regional

Influenza Surveillance Meeting

WHO, OIE, FAO May, 2013 Istanbul, Turkey

Unknown

International World Health Organization (WHO)

Working Group Meeting on PCR Protocols for the

Detection of Subtype Influenza A Viruses

WHO July 2-3, 2013

Geneva, Switzerland

Unknown

ToR: To collect, process, analyse, publish and disseminate data and information relevant to the designated specialty

8. Publication and dissemination of any information within the remit of the mandate given by the OIE that may be useful to Member Countries of the OIE (Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals:.................24

Lewis CE, Reising MM, Conrad SK, Fry AM, Hermann JR, Siev D, Gatewood DM. (2013).Evaluation of a non-invasive, inhalational challenge method for rabies vaccine potency assay. J Virol Methods. 2013 Jun; 190(1-2):49-52.

Hill RE Jr, Foley PL, Clough NE, Ludemann LR, Murtle DC. (2013). Translating Research into Licensed Vaccines and Validated and Licensed Diagnostic Tests. Dev Biol (Basel). 2013;135:53-8.

Srinivas GB, Walker A, Rippke BE. (2013). USDA Regulatory Guidelines and Practices for Veterinary Leptospira Vaccine Potency Testing. Biologicals. 2013 Jul 6. pii: S1045-1056(13)00063-8.



Ruby KW, Srinivas GB. (2013). Development of in vitro Assays for Measuring the Relative Potency of Leptospiral Bacterins Containing Serogroups Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona. Biologicals. 2013 Jul 16. pii: S1045-1056(13)00065-1.

Thiele TL, Stuber TP, Hauer PJ. (2013.) Detection of Clostridium sordellii hemorrhagic tocin (TcsH) positive strains and implications for diagnostics and regulation of veterinary vaccines. Vaccine. 2013 Oct 17; 31(44):5082-7.

Anderson TK, Nelson MI, Kitikoon P, Swenson SL, Korslund JA, Vincent AL. Population dynamics of co-circulating swine influenza A viruses in the United States from 2009-2012. Influenza Other Respir Viruses. 2013 Dec; 7 Suppl 4:42-51. doi: 10.1111/irv.12193.

Bracht AJ, Armién AG, Carrillo C, O'Hearn ES, Fabian AW, Moran KE, Lu Z, Ariyakumer DS, Rasmussen JM, Metwally SA. Isolation and characterization of a Cervidpoxvirus from a goitered gazelle (Gazella subgutturosa) from a zoologic park in Minnesota. J Zoo Wildl Med. 2013 Sep; 44(3):589-95.

Courtney SC, Susta L, Gomez D, Hines NL, Pedersen JC, Brown CC, Miller PJ, Afonso CL. Highly Divergent Virulent Isolates of Newcastle Disease Virus from the Dominican Republic are Members of a New Genotype that may have Evolved Unnoticed for Over Two Decades. J Clin Microbiol. 2013 Feb; 51(2):508-17. doi: 10.1128/JCM.02393-12. [Epub 2012 Nov 28]

Dubovi EJ, Hawkins M, Jr. Griffin RA, Johnson DJ, Ostlund EN. Isolation of Bluetongue virus from canine abortions. J Vet Diag Invest. 2013 Jul; 25(4):490-2. doi: 10.1177/1040638713489982. [Epub ahead of print]

Epperson S, Jhung M, Richards S, Quinlisk P, Ball L, Moll M, Boulton R, Haddy L, Biggerstaff M, Brammer L, Trock S, Burns E, Gomez T, Wong KK, Katz J, Lindstrom S, Klimov A, Bresee JS, Jernigan DB, Cox N, Finelli L; Influenza A (H3N2)v Virus Investigation Team. Human infections with influenza A (H3N2) variant virus in the United States, 2011-2012. Clin Infect Dis. 2013 Jul; 57 Suppl 1:S4-S11. doi: 10.1093/cid/cit272. [Swenson, SL. collaboration, not listed as author]

Jaques RS, Millien M, Flanagan KJ, Shaw J, Morales P, Pinto J, Pyburn D, Gonzalez W, Ventura A, Lefrancoise T, Pradel J, Swenson S, Jenkins-Moore M, Toms D, Erdman M, Cox L, Bracht AJ, Fabian A, Mohamed FM, Moran K, O'Hearn E, Carrillo C, Mayr G, White W, Metwally S, McIntosh M, Deng M. A field and lab investigation of viral disease of swine in the Republic of Haiti. Journal of Swine Health Prod. 2013; 21 (3) 130-138.

Kitikoon P, Gauger PC, Anderson TK, Culhane MR, Swenson SL, Loving CL, Perez DR, Vincent AL. Swine influenza virus vaccine serologic cross-reactivity to contemporary US swine H3N2 and efficacy in pigs infected with an H3N2 similar to 2011-2012 H3N2v. Influenza Other Respir Viruses. 2013 Dec 7. Suppl 4:32-41. doi: 10.1111/irv.12189.

Kitikoon P, Nelson MI, Killian ML, Anderson TK, Koster LG, Culhane MR, Vincent AL. Genotype patterns of contemporary reassorted H3N2 virus in U.S. swine. J Gen Virol. June 13. 94(6):1236-1241. doi: 10.1099/vir.0.51839-0. [Epub 2013 March 13]

Larocco M, Krug PW, Kramer E, Ahmed Z, Pacheco JM, Duque H, Baxt B, Rodriguez LL. 2013 A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine αVss6 Integrin has Increased Susceptibility to Foot and Mouth Disease Virus. J Clin Microbiol. Jun;51(6):1714-20. doi: 10.1128/JCM.03370-12. Epub 2013 Mar 20.

Lopez-Martinez I, Balish A, Barrera-Badillo G, Jones J, Nuñez-García TE, Jang Y, Aparicio-Antonio R, Azziz-Baumgartner E, Belser JA, Ramirez-Gonzalez JE, Pedersen JC, Ortiz-Alcantara J, Gonzalez-Duran E, Shu B, Emery SL, Poh MK, Reyes-Teran G, Vazquez-Perez JA, Avila-Rios S, Uyeki T, Lindstrom S, Villanueva J, Tokars J, Ruiz-Matus C, Gonzalez-Roldan JF, Schmitt BJ, Klimov A, Cox N, Kuri-Morales P, Davis CT, Diaz-Quiñonez JA. Highly Pathogenic Avian Influenza A (H7N3) Virus in Poultry Workers, Mexico, 2012. Emerg Infect Dis. 2013 Sep; 19(9). doi: 10.3201/eid1909.130087.

McCluskey BJ, McCluskey AM, Creekmore L, Schiltz J. Vesicular stomatitis outbreak in the southwestern United States, 2012. J Vet Diagn Invest. 2013 Sep; 25(5) 608-613. doi: 10.1177/1040638713497945. [Epub 2013 Jul 24]

McKimm-Breschkin JL, Barrett S, Pudjiatmoko, Azhar M, Wong FYK, Kim M, et al. (2013) I222 Neuraminidase Mutations Further Reduce Oseltamivir Susceptibility of Indonesian Clade 2.1 Highly Pathogenic Avian Influenza A(H5N1) Viruses. PLoS ONE 8(6): e66105. doi:10.1371/journal.pone.0066105

Nolting, J.M., P. Dennis, L. Long, L. Holtvoigt, D. Brown, M.J. King, W. Shellbarger, C. Hanley, M.L. Killian, R.D.



Slemons. 2013. Low Pathogenic Influenza A Virus Activity at Avian Interfaces in Ohio Zoos, 2006–2009. Avian Dis. 57(3):657–662.

Pedersen JC, Hines NL, Killian ML, Predgen AS, Schmitt BJ. Isolation of a virulent Newcastle disease virus from Confiscated LaSota Vaccine. Avian Diseases, 57 (2): 316-320. June 2013. doi: 10.1637/10420-101512.

Schroeder ME, Johnson DJ, Ostlund EN, Meier J, Bounpheng MA, Clavijo A. 2013. Development and performance evaluation of a streamlined method for nucleic acid purification, denaturation, and multiplex detection of Bluetongue virus and Epizootic hemorrhagic disease virus. J Vet Diagn Invest. Nov;25(6):709-19. doi: 10.1177/1040638713503654. Epub 2013 Oct 3.

Spackman E, Pedersen JC, McKinley ET, Gelb J Jr. Optimal specimen collection and transport methods for the detection of avian influenza virus and Newcastle disease virus. BMC Vet Res. 2013 Feb 22; 9:35. doi: 10.1186/1746-6148-9-35.

Thiele TL, Stuber TP, Hauer PJ. (2013.) Detection of Clostridium sordellii hemorrhagic toxin (TcsH) positive strains and implications for diagnostics and regulation of veterinary vaccines. Vaccine . 2013 Oct 17; 31(44):5082-7.

Vincent A, Awada L, Brown I, Chen H, Claes F, Dauphin G, Donis R, Culhane M, Hamilton K, Lewis N, Mumford E, Nguyen T, Parchariyanon S, Pasick J, Pavade G, Pereda A, Peiris M, Saito T, Swenson SL, Van Reeth K, Webby R, Wong F, Ciacci-Zanella J. Review of influenza A virus in swine worldwide: a call for increased surveillance and research. Zoonoses and Public Health. 2013 Apr. doi:10.1111/zph.12049.

Xu L, Hurtle W, Rowland JM, Casteran KA, Bucko SM, Grau FR, Valdazo-González B, Knowles NJ, King DP, Beckham TR, McIntosh MT. 2013. Development of a universal RT-PCR for amplifying and sequencing the leader and capsid-coding region of foot-and-mouth disease virus. J Virol Methods. Apr;189(1):70-6. doi: 10.1016/j.jviromet.2013.01.009. Epub 2013 Feb 1.

b) International conference presentations:.........................20

Avian Influenza/Newcastle disease February 25-March 1, 2013: Participation in the Multinational Influenza Seasonal Morbidity Study

(MISMS) meeting, Padova, Italy (Killian) March 7-8, 2013: V. Lazar, R. Sina, B. Moore, M. L. Killian, S. L. Swenson, R. Pogranichniy. Surveillance

Study of Influenza A Viruses of Swine In Indiana 2009 – 2012. Poster presentation at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses.

March 7-8, 2013: M.L. Killian, L. Koster, A. Janas-Martindale, S.L. Swenson, K. Toohey-Kurth, J. Easley, D. Lyman. “Isolation of influenza A (H3N2) from farmed mink”. Poster presentation at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses.

July 2-3, 2013: Presentation of the results of the 2012 OFFLU AI Ring Trial and current status of the 2013 OFFLU AI Ring Trial at the World Health Organization 6th PCR Working Group Meeting, Geneva, Switzerland (Hines)

March 25-27, 2013: Presentation of an overview of NVSL diagnostics and results at the Animal Influenza Viruses Countermeasures Working Group Meeting and Gap Analysis, Athens, GA, and co-led a working group with John Pasick (CFIA, Canada) to identify diagnostic gaps (Torchetti)

May 28-31, 2013: Invited speaker for the Joint ECDC/WHO European Regional Surveillance Meeting, Istanbul, Turkey; presentation on “Influenza and the human-animal intersect: what do we know and how do we work together” (Torchetti)

June 4-8, 2013: Presentation of “Laboratory networks contribute to ensuring diagnostic preparedness for avian influenza A(H7N9)” at the 16th International Symposium of the World Association of Veterinary Laboratory Diagnosticians (WAVLD) meeting in Berlin, Germany (Torchetti)

July 8-12, 2013: Representation at the NIAID Centers of Excellence for Influenza Research and Surveillance (CEIRS) meeting in Memphis, TN to share information related to the A(H7N9) virus from China and perspectives from avian health - continued participation enables NVSL to provide a significant resource for the avian testing and research in the USA (Torchetti)

FMD Carrillo, C. Vaccine Banks: The Official View. South America Commission for the Fight Against FMD

(COSALFA) Pre-Meeting April 15, 2013; Panama City, Panama. Carrillo, C. FMD Antigen Banks for the Protection of Free Countries. 40 th Annual Meetings, COSALFA

Panama City, Panama; April 15- 19, 2013 Carrillo, C. FMDV 2013 Activities, 8th Meeting of the OIE/FAO FMD Reference Laboratory Network,

November 14-15, 2013, Bangkok, Thailand



Swine Influenza Virus Lazar V, Sina R, Moore B, Killian ML, Swenson SL, and Pogranichniy R. Surveillance Study of Influenza A

Viruses of Swine In Indiana 2009 – 2012. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Swenson S, Killian ML, Pogranichniy RM, Strasser J, Lindstrom S, Kitikoon P, House J, Lenz S, Koster L, Marsh B, Davis WG, Berman L, Vincent A, Richards S, Glazier M, Janas-Martindale A, and Tomlinson S. Outbreak of Influenza A (H3N2) in People and Pigs at County Fairs. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Killian ML, Koster L, Janas-Martindale A, Swenson SL, Toohey-Kurth K, Easley J, and Lyman D. Isolation of influenza A (H3N2) from farmed mink. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Garten R, Balish A, Killian ML, Swenson S, Bowman A, Davis C, Davis W, Shu B, Berman L, Barnes J, Lindstrom S, Villanueva J, and Klimov A. Characterization of influenza A(H3N2)v at county and state agricultural fairs in the U.S. Presented at the XV International Conference on Negative Strand Viruses, June 16-21, 2013.

Swenson S, Pyburn D, Korslund JA, Tomlinson S, Kasari EM, Vincent A, and Schiltz J. National surveillance for swine influenza virus in the United States, 2009-present. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Anderson TK, Nelson MI, Kitikoon P, Swenson SL, Korslund JK, and Vincent AL. Spatial and temporal dynamics of influenza A in North American Swine. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Kitikoon P, Gauger PC, Schlink SN, Bayles DO, Culhane MR, Darnell D, Webby RJ, Lager KM, Swenson SL, Klimov A, and Vincent AL. Pathogenicity and transmission of the novel A(H3N2)v influenza virus isolated from humans in experimentally inoculated pigs. Presented at the International Society for Influenza and other Respiratory Virus Diseases 2nd International Symposium on Neglected Influenza Viruses, March 7-8, 2013.

Swenson SL and Korslund JK. USDA influenza A virus surveillance program in swine. Presented at the OFFLU Swine Influenza Virus Group meeting, March 27-28, 2013.

Lewis NS, Russell CA, Burke DF, Brown IH, Chen H, Claes F, Dauphin G, Donis R, Culhane M, Hamilton K, Mumford E, Nguyen T, Nuansrichy B, Pasic J, Pavade G, Pereda A, Peiris M, Saito T, Swenson S, Van Reeth K, Vincent A, Webby R, Wong F, and Ciacci-Zanella J. Quantifying the global antigenic and genetic evolution in swine influenza A viruses and evaluating the relative zoonotic potential in humans. Presented at Options for the Control of Influenza VIII, September 5-10, 2013.

c) National conference presentations:................................15

Avian Influenza/Newcastle disease January 14-17, 2013: Participation in the International Influenza Networks meeting, Scottsdale, AZ

(Hines) February 19-22, 2013: Representation at the Live Bird Market Working Group Meeting in Seattle, WA

(Pedersen) March 11-12, 2013: Presentation of an Update of Avian Influenza and Newcastle Disease at the North

Central Avian Disease Conference, St. Paul, MN (Pedersen) March 25-27, 2013: Presentation on Trends in Veterinary Diagnostics at the Western Poultry Disease

Conference, Sacramento, CA (Pedersen) May 7-10, 2013: Representation at the USDA-NIH Multinational Influenza Seasonal Morbidity Study

workshop (Killian) May 20-23, 2013: Conducted a NAHLN site visit at the Charles C. Allen Biotechnology Laboratory and the

University of Delaware Lasher Laboratory in DE (Killian) May 28-31, 2013: Participation in the Sequencing Finishing Meeting Santa Fe, NM (Killian) June 17-20, 2013: Representation at the NPIP Official State Agency/General Conference Committee

meeting, and presentation on the implementation of 11 swab pools (Killian) July 19-24, 2013: Representation at the AAAP/AVMA meetings and subcommittee meetings in Chicago,

IL (Killian, Torchetti) – presentation of “Atypical Turkey Newcastle Disease in the Upper Midwest Part II: Virus characterization and vaccinal protection” (Killian)

October 21, 2013, 117th Annual Meeting of the United States Animal Health Association; Presentation of annual NVSL Diagnostic Reports for Avian Influenza and Newcastle disease; San Diego, CA (Torchetti)

Bluetongue: 117th Annual Meeting of the United States Animal Health Association, Bluetongue and Related Orbivirus



Committee; October 17023, 2013; San Diego, CAFMD : 117th Annual Meeting of the United States Animal Health Association, October 17023, 2013; San Diego, CASwine Influenza Virus: Swenson S, Killian ML, Pogranichniy RM, Strasser J, Lindstrom S, Kitikoon P, House J, Lenz S, Koster L,

Marsh B, Davis WG, Berman L, Vincent A, Richards S, Glazier M, Janas-Martindale A, and Tomlinson S. Outbreak of Influenza A (H3N2) in People and Pigs at County Fairs. Presented at the American Association of Swine Veterinarians Annual Meeting, March 2-5, 2013.

Killian M.L., T. Anderson. NGS sequencing of Influenza A Virus at USDA. Presented at the FIC-IRD-USDA-National Pork Board Sequencing and Epidemiology Workshop, May 7-10, 2013.

Swenson SL. Influenza A infection in swine (IAV-S). Presented at the Arkansas One Health Conference, November 21, 2013.

d) Other:..............................................................................Links to websites for additional information

(Provide website address or link to appropriate information)

AI: http://www.aphis.usda.gov/vs/nahss/poultry/index.htmFMD: http: www.ncbi.nlm.nih.gov; Genebank records KC440881-KC440884EEE/WEE: http://www.aphis.usda.gov/vs/nahss/equine/ee/EIA: http://www.aphis.usda.gov/vs/nahss/equine/eia/index.htmPRV[Aujesky’s}: http://www.aphis.usda.gov/vs/nahss/swine/index.htmSIV: http://animalhealth.we.aphis.gov/observer/home/Lists/Posts/Post.aspx?ID=289VSV: http://www.aphis.usda.gov/vs/nahss/equine/vsv/index.htmWNV: http://www.aphis.usda.gov/vs/nahss/equine/wnv/index.htm

_______________


http://www.aphis.usda.gov/vs/nahss/equine/wnv/index.htm

http://www.aphis.usda.gov/vs/nahss/equine/vsv/index.htm

http://animalhealth.we.aphis.gov/observer/home/Lists/Posts/Post.aspx?ID=289

http://www.aphis.usda.gov/vs/nahss/swine/index.htm

http://www.aphis.usda.gov/vs/nahss/equine/eia/index.htm

http://www.aphis.usda.gov/vs/nahss/equine/ee/

http://www.ncbi.nlm.nih.gov/

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