Age and growth analysis of the shortfin mako, Isurus oxyrinchus, in the western and central

North Pacific Ocean

西部和中部北太平洋海域灰鯖鮫之年齡成長分析

Yasuko Semba & Hideki Nakano & Ichiro AokiEnviron Biol Fish (2009) 84:377–391

speaker ：陳怡婷

Shortfin Mako

Kingdom AnimaliaPhylum ChordataClass ChondrichthyesSubclass ElasmobranchiiOrder LamniformesFamily LamnidaeGenus IsurusSpecies oxyrinchus

中文名 灰鯖鮫圖片來源 http://fishdb.sinica.edu.tw/chi/showpic.php?science=Isurus%20oxyrinchus

資料來源 http://fishdb.sinica.edu.tw/chi/newsearch/search.asp?search=order00

IntroductionIntroduction

Biology

• Habitats : Ocean 、 Deep Sea 、 Coastal 

• Habitats Depth : 0 - 740 M 

• Max Lenth  : 400 cm 

• Size at birth estimated to be about 60-70 cm in TL. • Ovoviviparous, with a uterine cannibal.

高級消費著

消費者

生產者

http://fishbase.sinica.edu.tw/Photos/ThumbnailsSummary.php?ID=752

r-organisms K-organisms short-lived long-lived

small adult size large adult size

weak strong or well-protected

waste a lot of energy energy efficient

have large litters have small litters

reproduce at an early age reproduce at a late age

fast maturation slow maturation

strong sex drive weak sex drive

small size at birth large size at birth

Characteristic

• Wide distribution, and prefers temperatures of 17 to 22°C. (Casey and Kohler 1992)

• Migration • Sexual/ontogenetic stage segregation

Distribution

資料來源 http://www.aquamaps.org/receive.php

Sustainable Development

• The recent and rapid collapse of large predatory fish populations is a global concern (Myers and Worm 2003, 2005).

• Information on life history parameters derived from age and growth analyses plays an integral role. Age determination is an important component of population analyses.

• In addition, the growth curves generated in this study were compared to those for populations in other geographic regions.

Materials and methodsMaterials and methods

Sample collection

• Sampling period : 1992~2005 by the Japanese research vessels and tuna longline training vessels.

• Sampling amount : 275 individuals (128 males and 147 females).

• Sampling site : western and central North Pacific Ocean.

Fig. 1 Sampling locations for the shortfin mako (Isurus oxyrinchus) in the western and central North Pacific Ocean. Each point denotes a position where samples were collected.

Fig. 2 Length frequency of the specimen used for the analysis

species.wikimedia.org/.../Images/test

PCL ( cm )

TL ( mm )

DL ( mm )

FL ( mm )

DL, TL, FL with PCL

PCL : 57.2 to 186.5 cm

Step

Sex determination

Measure PCL

Excise the vertebral column above the gill slits

Froze the vertebral column at −40°C

Vertebral processing

Boil the vertebrae

Store it in 70% ethanol

Cut off a single centrum

Soak it in 6N NaOH

Scrub it with apolishing buff

Each centrum is cutinto two longitudinal sections after drying

The halfcut centra with the focus were used for analysis after washing away the bone particle and drying for 1 day

Four techniques to enhance vertebral growth bands

1. shadowing (half-cut centra; Francis and Maolagáin 2000)

2. alizarin staining (section;Berry et al. 1977)

3. silver nitrate staining (whole centra; Stevens 1975)

4. soft X-radiography (whole or half-cut centra; Cailliet et al. 1983)

Method of age determinationMethod of age determination

shadowing alizarin staining

silver nitrate staining

soft X-radiography

Vertebral half-cut centra sagittal section whole centra whole or half-cut centra

Growth bands

convex

concave

lightly stained (opaque)

deeply stained (translucent)

translucent region

opaque region

Spending time

shorttest longer longer shorter

analysis more precision and clarity

less information less information overestimation

The shadowing method

1. Simplicity

2. Visual clarity

3. Relative abundance of information amount

4. Less time-consuming process

Fig. 3 Half of a vertebral centrum with lateral illumination from fiber-optic sources. The triangles indicate a convex structure or growth band. This example is from a 137 cm PCL female, with a vertebral centrum radius of 9.5 cm, and an estimated age of 4.6 years

Age determination

• In this way, the convex and concave structures were observed as light and dark regions, respectively. These structures correspond to the opaque (convex) and translucent (concave) regions observed in the sagittal section.

• The vertebrae of neonates, which displayed only a birthmark, were excluded from this analysis.

CR with PCL

• The centrum radius (CR) is the distance from the focus to the edge of the centrum perpendicular to the direction of the cutting plane. The CR was measured to the nearest 0.01 mm

CR

Fig. 6 Age-bias plot of 275 shortfin mako vertebral counts from two independent readings by the first author. The open and solid mark indicate the observed counts and the mean counts assigned by the second count for all fish which was assigned a given count in the first count up to 12, respectively. The vertical bars for the solid mark up to the first count of 11 indicate the 95% confidence interval. The one to one equivalence line is also represented.

PA= [No. agreed/No. read]*100

Assuming that annuli (convex structures) are formed soon before or after 1 December, age in years was calculated as follows:

x = the number of convex structurey = the month when the individual was caught (1≤y≤12).

Lt = PCL at time t (cm)

L0 = size at birth (cm)(74 cm TL) (Joung and Hsu 2005)

L∞ =asymptotic length (cm) K = brody growth constant (year−1).

VBGE with size at birth

Results

Male : 53~240 cm (PCL)

Female : 57~300cm (PCL)

the allometric equation for regression analysis

Akaike’s Information Criterion

AIC = n ln(MSE) + 2K

• model N (no bandperiodicity) : 312.44

• model A (annual periodicity) : 271.89

• model B (biannual periodicity) : 316.24

The estimated growth equations

t = age (years)

The estimated S.E. of L∞ for females was larger than that for males, whileS. E. of K for males was larger than for females

DiscussionDiscussion

Growth

Fig. 7 Estimated von Bertalanffy growth curves for female and male shortfin mako in the western and central North Pacific Ocean

PCL : 308 cm

FL : 370 cm

Growth band formation

• Convex dominant in the winter and concave dominant in the summer.

• Neonates captured in May and June suggested that the formation of the first convex concentric structure (birthmark) occurs after parturition.

• Branstetter (1987)

• We determined that the growth increment between the birthmark and the second annulus is approximately 7 months.

Fig. 8 Comparison of growth curves to those reported in previous studies for a males and b females. The curves reported by Pratt and Casey (1983) were recalculated assuming one growth band pair formation per year

Growth comparisons among geographical regions

• Eastern North Pacific (Cailliet and Bedford 1983; Ribot-Carballal et al. 2005)

• Atlantic (Pratt and Casey 1983); (Natanson et al. 2006)

• South Pacific (Bishop et al. 2006)

Conclusion

• Our data from the western and central Pacific Ocean indicate that shortfin makos in this area grow slowly and mature late, and our results provide insight into the population structure.

• A better understanding of the age structure in a unified regional framework will allow us to construct effective guidelines for the assessment and management of the shortfin mako population.

Thak you for your attention !Thak you for your attention !