agenda’ - amazon s3twin+workshop+a… · agenda ’! introduc)on ... hi-a-drbl*13 02 01 02 02 01...
TRANSCRIPT
Agenda
§ Introduc)on to (5 mins)
§ Hands on workflows in HLA Twin (15 mins) § High Quality Holotype HLA X4 data § Novel alleles with IMGT/HLA v3.10
§ Export consensus
§ Novel alleles with IMGT/HLA v3.15
§ Q&A! (10 mins)
Omixon HLA Twin™ (So4ware)
§ SoPware component of Holotype HLA
§ Automated genotyping aPer MiSeq run
§ Preconfigured Holotype HLA-‐specific seVngs
§ Two algorithms for determining HLA genotypes – Consensus Genotyping (Assembly) – Sta)s)cal Genotyping (Alignment to IMGT/HLA)
§ Traffic light system for data interpreta)on and workflow management
PASSED
WARNING
FAILED
3 IT Implementa<ons of Twin
Manual launch on a Server
Fully automated on a Server
HLA Twin on a laptop or PC
Traffic Light #1 Dual Algorithm Concordance
Fully concordant
Concordant to 2nd field
Concordant to 1st field
Discordant
Traffic Light #2 Locus-‐level Quality Check
All QC metrics passed
At least 1 QC warning
At least 1 QC failed