agranco corp. usa

AGRANCO CORP. USA

MANUFACTURER OF ENZYMES , PROBIOTICS, ANTIBODIES , MYCOTOXIN ADSORBANTS ,

CHELATED AND MICROENCAPSULATED MINERALS, NATURAL INSECTICIDES AND

FERTILIZERS, AND GERMICIDES FOR THE FEED, AQUACULTURE , AGRO. FOOD PROCESSING AND

SUGAR MANUFACTURING INDUSTRIES,

RESPECTIVELY. WWW.AGRANCO.COM

AGRANCO CORP. USA

Our Mission StatementOur Mission Statement““The Good Action of Today Make For a Better The Good Action of Today Make For a Better

TomorrowTomorrow””

Our mission is to provide and serve the world agro-Our mission is to provide and serve the world agro-industry with our quality products , in order to industry with our quality products , in order to improve the quality of live of peoples throughout improve the quality of live of peoples throughout the world. We try to accomplish this by the the world. We try to accomplish this by the manufacture of the best quality products and and manufacture of the best quality products and and their distribution through our network of their distribution through our network of distributors in all parts of the world. distributors in all parts of the world.

Las buenas acciones de hoy hacen un mañana mejor

¿Who are we?¿Who are we?

Agranco Group is a manufacturing and sale organization with Agranco Group is a manufacturing and sale organization with corporate offices in Coral Gables, Fl. USA, and manufacturing corporate offices in Coral Gables, Fl. USA, and manufacturing organizations in Mexico and the USA.. AGRANCO has a distribution organizations in Mexico and the USA.. AGRANCO has a distribution network covering Mexico, USA ,Central and South America, network covering Mexico, USA ,Central and South America, Europe , The Middle East , North Africa and Asia. All are dedicated Europe , The Middle East , North Africa and Asia. All are dedicated to the distribution and service of our products, with their own staff to the distribution and service of our products, with their own staff of Engineers , Technical Specialists and phD’ in each area of activity of Engineers , Technical Specialists and phD’ in each area of activity and expertise. These areas are: Feed and Related Industries, Food and expertise. These areas are: Feed and Related Industries, Food Processing Sanitization, Sugar Manufacturing and Agricultural Processing Sanitization, Sugar Manufacturing and Agricultural Industries. Our products are unique for each industry, of our own Industries. Our products are unique for each industry, of our own formulation and brand names. Each product has been thoroughly formulation and brand names. Each product has been thoroughly researched in laboratory and field trials. researched in laboratory and field trials.

We invite you to evaluate them and become part of our list of We invite you to evaluate them and become part of our list of satisfiedsatisfied

PHYTEX 500PHYTEX 500

PhytasePHYTEX 500………500.000 U*AGR

U* = Units of activityDosage: 100 gr. Per MT of feed

Saves 0.1% of inorganic phosphorous and 1.5% of protein per MT of feed

All ingredients are approved by FDA and the Association of American Feed Control Officials/ Official publication / page No.

998 chapter 30 Eznymes .

What IS PHYTEX 500What IS PHYTEX 500 Phytase is an enzyme that is found in nature, Phytase is an enzyme that is found in nature,

principally, in plants. It is formed in its growing principally, in plants. It is formed in its growing stage to supply phosphorous to the plant during stage to supply phosphorous to the plant during that stage. Phytase is also found in the intestinal that stage. Phytase is also found in the intestinal moccus of the small intestine in most animals. In moccus of the small intestine in most animals. In the case of micro-organisms, it is found in the case of micro-organisms, it is found in Aspergillus sp. and in yeast. PHYTEX 500 is Aspergillus sp. and in yeast. PHYTEX 500 is obtained by a natural fermentation process , from obtained by a natural fermentation process , from Aspergillus Nigger, without modification of its Aspergillus Nigger, without modification of its genetic structure. (NON-GMO)genetic structure. (NON-GMO)

Mode of ActionMode of Action Inositol Hexaphoshate, (IH), is present in Inositol Hexaphoshate, (IH), is present in

cereals and raw materials of vegetable cereals and raw materials of vegetable origin, at 1% to 3%. (IH) contains natural origin, at 1% to 3%. (IH) contains natural phosphoric acid at 60% to 80%. Example: in phosphoric acid at 60% to 80%. Example: in Wheat (IH): 0.6% to 1.4% (w/w) ; Soy Flakes: Wheat (IH): 0.6% to 1.4% (w/w) ; Soy Flakes: (IH): 1.5% to 1.8% (w/w). Bran: (IH): 2.6% to (IH): 1.5% to 1.8% (w/w). Bran: (IH): 2.6% to 5.4% (w/w).Prot.Con: (IH):2.0% to 3.0%.(w/w) 5.4% (w/w).Prot.Con: (IH):2.0% to 3.0%.(w/w) Phytase breaks the (IH) molecule freeing the Phytase breaks the (IH) molecule freeing the phosphoric acid present , making it bio-phosphoric acid present , making it bio-available to the animal in a natural form , available to the animal in a natural form , and reducing the need for inorganic and reducing the need for inorganic phosphorous.phosphorous.

PHYTEX 500 Las buenas acciones de hoy hacen un mañana mejor

PHYTEX 500 savings in formualtion, units of activity and economic advantages

When PHYTEX 500 is added to feed , at recommended levels, it allows for 1.5% savings in protein, 0.1% savings

inorganic phosphorous and 3.25% savings in energy. These savings represent at least a net savings of 600% of its

inclusion cost in formulation, per MT of feed. One unit of activity of PHYTEX 500 is defined as “ the amount of enzyme required to liberate 1 micro mole of inorganic phosphorous

per minute as measured by its method of quantification”. One unit of activity AGR U* is defined as “the amount of enzyme

required to produce (together) : an increase of : 1.5% soluble protein (w/w), 0.1% (w/w) liberated at pH 5 and 37ºC per

gram of standard formulated feed. PHYTEX 500 contains 5,000 AGR U* per gram.

.


Other benefits:Other benefits:Inositol Hexaphosphate (IH), bonds with minerals Inositol Hexaphosphate (IH), bonds with minerals , such as iron, calcium and magnesium in feeds, , such as iron, calcium and magnesium in feeds, reducing their bio-availability to the animals. (IH) reducing their bio-availability to the animals. (IH) also form insoluble molecular complexes with also form insoluble molecular complexes with proteins. These complexes prevent digestive proteins. These complexes prevent digestive enzyme activities , reducing digestibility of feeds enzyme activities , reducing digestibility of feeds and some enzyme emmulsifying functions. These and some enzyme emmulsifying functions. These molecular complexes, create the necessity of molecular complexes, create the necessity of inorganic phosphorous , as an additive in rations. inorganic phosphorous , as an additive in rations. Some of the inorganic phosphorous included in Some of the inorganic phosphorous included in rations is excreted in feces , creating rations is excreted in feces , creating environmental contamination in swine operations.environmental contamination in swine operations.


Inclusion of PHYTEX 500 in feed rationInclusion of PHYTEX 500 in feed ration100 grams per MT of feed. PHYTEX 500 can be pre-100 grams per MT of feed. PHYTEX 500 can be pre-mixed or added directly into the mixer. 100 grams of mixed or added directly into the mixer. 100 grams of PHYTEX 500 provide 500,000 AGR U* per MT of feed. PHYTEX 500 provide 500,000 AGR U* per MT of feed. Characterisitics:Characterisitics:Activity vs. Temperature: 100% Act.@ 60°C. (Range: Activity vs. Temperature: 100% Act.@ 60°C. (Range: 48°C a 68°C). Activity vs. pH: 100% Act. @ pH 6. (Range 48°C a 68°C). Activity vs. pH: 100% Act. @ pH 6. (Range de 5.5 a 7). However, PHYTEX 500 can be pelletized at de 5.5 a 7). However, PHYTEX 500 can be pelletized at maximum: 86º C during 30 seconds. Enzyme loss: 2% to maximum: 86º C during 30 seconds. Enzyme loss: 2% to 5%.5%.It can also be mixed with water (100% soluble) and It can also be mixed with water (100% soluble) and added by spray after pelleting.added by spray after pelleting.Packaging:Packaging:PHYTEX 500 is packed in poly plastic bags in 20 kg boxes PHYTEX 500 is packed in poly plastic bags in 20 kg boxes or in 20 kg paper bags.or in 20 kg paper bags.


Use and formulation guide for PHYTEX 500:Use and formulation guide for PHYTEX 500:

PHYTEX 500 saves protien, energy and PHYTEX 500 saves protien, energy and phosphorous values in feed rations. The follwoing phosphorous values in feed rations. The follwoing corrective values (reduction) should be taken into corrective values (reduction) should be taken into account when formulation with PHYTEX 500: account when formulation with PHYTEX 500: ahorra valores de proteína y fósforo en la ración. ahorra valores de proteína y fósforo en la ración. Proteins Proteins 1.50 %1.50 %Energy Energy 3.25 %3.25 %Phosphorous 0.10 %Phosphorous 0.10 %


Increase in phosphorus values with PHYTEX 500:Increase in phosphorus values with PHYTEX 500:

Standard digestibility reference in %: 0.495 of liberated Standard digestibility reference in %: 0.495 of liberated phosphorous at 0.44% phosphorous phosphorous at 0.44% phosphorous

Digestibility per uniform dosage of PHYTEX 500, Digestibility per uniform dosage of PHYTEX 500, %:0.630%%:0.630%

Increase in digestibility of phosphorous %: 0.135 Increase in digestibility of phosphorous %: 0.135 Disgestible liberated phosphorous, %: 0.0594. Calculated Disgestible liberated phosphorous, %: 0.0594. Calculated

as follows: (0.630-0.495)*0.44 as follows: (0.630-0.495)*0.44 Disgestible liberated phosphorous, gr./Kg.: 0.594 gr./Kg. Disgestible liberated phosphorous, gr./Kg.: 0.594 gr./Kg. Inorganic phosphorous bio-available replaced by PHYTEX Inorganic phosphorous bio-available replaced by PHYTEX

500: ( Defluorinated Phosphate) , %: 0.100500: ( Defluorinated Phosphate) , %: 0.100


PHYTEX 500

Nutrient BROILERS Pullets Layer/Breeders

Starter Grower/Finisher

Phosphorous 1,590 1,675 1,870 2,225

Calcium 1,590 1,675 1,870 4,455

EM, Kcal/kg 173,555 170,685 192,780 272,735

Proteins 5.041 5,032 5,610 8.020

Lisine 260 259 288 413

Metionine 39 39 38 63

Cistine 80 80 89 126

M+C 126 125 154 299

Tryptofan 23 23 26 36

Treonine 171 170 190 272

Valine 203 203 226 322

Isoleucine 221 220 245 351

Leucine 344 343 382 546

Arginine 268 267 318 453

Fenilalanine 236 234 350 371

Histidine 165 164 283 262

SwineNUTRIENT 100grams

PHYTEX 500 equivalen to

Matrix Values Nutritional %

Fósforo digest. 11.500 g 1.150

Calcium 10.000 g 1.000

Lisinae* 800 g 80

Metionine * 250 g 25

Cistine * 300 g 30

Treonine* 500 g 50

Triptofane * 300 g 30

Isoleucine * 500 g 50

Proteíne Crude * 20.000 g 2.000

Metabolizable Energy** 95.000 Kcal 95.000


• * = Ideal apparent digestibility; ** = based on crude protein

Matrix interpretationMatrix interpretation::

The hydrolitic action of PHYTEX 500 on Inositol Hexaphosphate (IH) The hydrolitic action of PHYTEX 500 on Inositol Hexaphosphate (IH) increases the bio-availability of phosphorous , and also that of other increases the bio-availability of phosphorous , and also that of other nutrients in feed. The presence of (IH) bonds to minerals and proteins nutrients in feed. The presence of (IH) bonds to minerals and proteins forming insoluble complexes , decreasing the bioavailabilty of these forming insoluble complexes , decreasing the bioavailabilty of these nutrients in feeds. Breaking down (IH), not only liberate phosphorous but nutrients in feeds. Breaking down (IH), not only liberate phosphorous but also other nutrients as expressed in the accompanied matrix.also other nutrients as expressed in the accompanied matrix.

The values that appear in the matrix above, are percentages and only The values that appear in the matrix above, are percentages and only take into consideration the hydrolitic action of PHYTEX 500. The take into consideration the hydrolitic action of PHYTEX 500. The percentages are directly related with the rate of inclusion of PHYTEX 500 percentages are directly related with the rate of inclusion of PHYTEX 500 in feed. (grams/MT). in feed. (grams/MT).

To determine the % of additional nutritional values made available by the To determine the % of additional nutritional values made available by the hydrolitic action of PHYTEX 500, the following formula is used:hydrolitic action of PHYTEX 500, the following formula is used:

The nutritional value is equivalent to:The nutritional value is equivalent to:Nutrient (%) matrix value X (Dosage in grams) PHYTEX 500 / MT of feedNutrient (%) matrix value X (Dosage in grams) PHYTEX 500 / MT of feedDevided by 1,000,000 gramos / TMDevided by 1,000,000 gramos / TMMaking this calculation per each matrix nutrient, determines the Making this calculation per each matrix nutrient, determines the increment provided by PHYTEX 500, and is equivalent to the increment provided by PHYTEX 500, and is equivalent to the corresponding savings per nutrient.corresponding savings per nutrient.

PHYTEX 500

Guaranteed CompositionGuaranteed Composition

INGREDIENTSINGREDIENTS Units of Activity Units of Activity PERCENTAGE PERCENTAGE Fermentation Products Fermentation Products Aspergillus niger (Enzymes)Aspergillus niger (Enzymes) 30% 30% Units of Activity AGR U* Units of Activity AGR U* 500,000 /100 Grams500,000 /100 Grams

Aspergillus OrizaeAspergillus OrizaeFermentation products 40% Fermentation products 40%

Carrier- Maltodextrine 30% Carrier- Maltodextrine 30% Total........................l00%Total........................l00%


PHYTEX 500 AGRU (Unit of Activity) PHYTEX 500 AGRU (Unit of Activity) DEFINITIONDEFINITION

One unit of activity of PHYTEX 500 is defined as the One unit of activity of PHYTEX 500 is defined as the quantity of PHYTEX 500 required to liberate one quantity of PHYTEX 500 required to liberate one micro-mole of inorganic phosphorous per minute micro-mole of inorganic phosphorous per minute according to the Phytex 500 method of analysis. according to the Phytex 500 method of analysis. One unit of AGR U* (Unit of activity) is the quantity of One unit of AGR U* (Unit of activity) is the quantity of enzyme , that together with phytase in PHYTEX 500, enzyme , that together with phytase in PHYTEX 500, is required to increment 1.5% of soluble protein (w/w) is required to increment 1.5% of soluble protein (w/w) , 3.25 % energy (w/w) and 0.1% phosphorous (w/w) in , 3.25 % energy (w/w) and 0.1% phosphorous (w/w) in one gram of feed at pH 5, at 37ºC. one gram of feed at pH 5, at 37ºC. PHYTEX 500 contains 5,000 AGR U* per gram.PHYTEX 500 contains 5,000 AGR U* per gram.


UNITS OF ACTIVITYUNITS OF ACTIVITYPhytase Activity: Phytase Activity: Approximately 500 units / Approximately 500 units / gramgram Measuring conditions : Substrate Inositol Measuring conditions : Substrate Inositol Hexaphoaphate Hexaphoaphate

(Final Conc.. = 6. OmM)(Final Conc.. = 6. OmM)

PH -PH - 6.0 6.0 Temperature - 37 °CTemperature - 37 °C

Reaction time - 30 MinutosReaction time - 30 Minutos Unit definition:Unit definition: One unit liverates one micromole per One unit liverates one micromole per minute minute under the PHYTEX 500 method of under the PHYTEX 500 method of analysisUnaanalysisUna Please refer to: The Journal of Food Science. Vol.24 (No. 4), 1977Please refer to: The Journal of Food Science. Vol.24 (No. 4), 1977

PHYTEX 500

PHYTASE METHOD OF ANALYSISPHYTASE METHOD OF ANALYSIS

1.1. Pipet 1 ml of solution of substrate in test tube 18 X Pipet 1 ml of solution of substrate in test tube 18 X 180 MM. Pre.-incubate in water bath at 37ªC. 180 MM. Pre.-incubate in water bath at 37ªC.

2.2. At zero time, rapidly pipet 0.1 ml of enzyme solution At zero time, rapidly pipet 0.1 ml of enzyme solution in substrate and mix, agitating it. Incubate at 37ªC in substrate and mix, agitating it. Incubate at 37ªC for exactly 30 mins. Adding 3 ml of 1M for exactly 30 mins. Adding 3 ml of 1M triachloroacetic acid, mixing it slightly. (Solution A). triachloroacetic acid, mixing it slightly. (Solution A).

3.3. Pipet 1 ml. Of solution A and 7 ml of water in test Pipet 1 ml. Of solution A and 7 ml of water in test tube (18 X 180 mm) and mix agitating . Pipet 1.5 ml tube (18 X 180 mm) and mix agitating . Pipet 1.5 ml of solution of ascorbic acid and mix agitating. Let of solution of ascorbic acid and mix agitating. Let rest for 20 mins at 37ªC. rest for 20 mins at 37ªC.

4.4. After incubation , determine the absorbance of the After incubation , determine the absorbance of the solution in cell (1-cm), at 850 nm with spectrometer , solution in cell (1-cm), at 850 nm with spectrometer , using water (T).using water (T).

PHYTEX 500

MÉTHOD OF ANALYSIS OF PHYTASE ACTIVITYMÉTHOD OF ANALYSIS OF PHYTASE ACTIVITY Pipet 1 ml. of substrate solution, 3 ml of 1 M trichloroacetic acid Pipet 1 ml. of substrate solution, 3 ml of 1 M trichloroacetic acid

and 0.1 ml of enzymatic solution in test tube of 18 X 180 mm. and 0.1 ml of enzymatic solution in test tube of 18 X 180 mm. for blank. Then prepare a blank of the same in the same form for blank. Then prepare a blank of the same in the same form as the sample, and determine the absorbance of blank (B). as the sample, and determine the absorbance of blank (B).

Pipet standard solution, 7 ml. Of water and 1.5 ml. Of ascorbic Pipet standard solution, 7 ml. Of water and 1.5 ml. Of ascorbic acid. Then prepare a standard in the same manner as the acid. Then prepare a standard in the same manner as the sample and determine absorbance (ST). sample and determine absorbance (ST).

Activity Activity = T-B X 0.2631 X 4.1 X 1 X n = T-B X 0.2631 X 4.1 X 1 X n (ST-SB) (1.0) (30) (0.1)(ST-SB) (1.0) (30) (0.1)T = Absorbance of enzymatic solutionT = Absorbance of enzymatic solutionB = Absorbance of blank enzymatic solutionB = Absorbance of blank enzymatic solutionST = Absorbancia of standard solutionST = Absorbancia of standard solutionn = Dilution factorn = Dilution factor0.2631 = u mole of inorganic phosphorous in 1 ml of standrad solution. 0.2631 = u mole of inorganic phosphorous in 1 ml of standrad solution.

PHYTEX 500

L D50 OF ENZYMES FOR PHYTEX 500L D50 OF ENZYMES FOR PHYTEX 500

The following LD values were obtained at 10 times the The following LD values were obtained at 10 times the recommended concentration of PHYTEX RATS:recommended concentration of PHYTEX RATS:

MALESMALES FEMALES FEMALES

OralOral 25,000 mg / Kg and more25,000 mg / Kg and more 25,000 mg / KG 25,000 mg / KG and moreand moreSubcutaneousSubcutaneous 6,420 mg / Kg 6,420 mg / Kg 6,350 mg / Kg6,350 mg / KgIntra-peritonealIntra-peritoneal 3,170 mg/Kg3,170 mg/Kg 3,170 mg/Kg3,170 mg/Kg

RATRATMALESMALES FEMALES FEMALES

OralOral 20,000 mg/Kg and more20,000 mg/Kg and more 20,000 mg / Kg 20,000 mg / Kg and moreand moreSubcutaneous Subcutaneous 12,380 mg/Kg12,380 mg/Kg 10,700 mg/Kg10,700 mg/KgIntraperitonealIntraperitoneal 3,280 mg / Kg3,280 mg / Kg 3,450 mg / Kg3,450 mg / Kg

PHYTEX 500

PHYSICAL , CHEMICAL AND BIOLOGICAL PROPERTIESPHYSICAL , CHEMICAL AND BIOLOGICAL PROPERTIES

1.1. Physical properties: PHYTEX 500 is a beige powder with odor. Physical properties: PHYTEX 500 is a beige powder with odor. – Segregation: 1Segregation: 1– Non compacted density gr/cm3: 0.71 Non compacted density gr/cm3: 0.71 – Compacted densty gr/cm-: N/A Compacted densty gr/cm-: N/A – Cohesiviness: 1.07 Cohesiviness: 1.07 – Compresivity %: 14 Compresivity %: 14 – Ángle of repose: 32.9 Ángle of repose: 32.9 – Solubility: 100% soluble in water. Solubility: 100% soluble in water. – Moisture absorption: 2% o Malla: 200Moisture absorption: 2% o Malla: 200

2.2. Optimum pH: Amylase: 5.0, Protease: 3.0, Celullase: 4.5, Optimum pH: Amylase: 5.0, Protease: 3.0, Celullase: 4.5, Lipase:7.0 , Pectinase:4.0, Xylanase: 5.0, Lactase: 5.5, Lipase:7.0 , Pectinase:4.0, Xylanase: 5.0, Lactase: 5.5, Phytase: 6.0Phytase: 6.0

PHYTEX 500

BIOLÓGICAL, CHEMICAL AND PHYSICAL BIOLÓGICAL, CHEMICAL AND PHYSICAL PROPERTIES PROPERTIES

3.3. Phytase identification test Phytase identification test 4.4. Activity in breaking down (IH) Inositol Activity in breaking down (IH) Inositol

Hexaphosphate made to liberate Phitic Hexaphosphate made to liberate Phitic Acid: PASS .Acid: PASS .

5.5. Identifications test were made with Identifications test were made with previously describe procedures. previously describe procedures.

6.6. Properties: PH vs. Activity, pH vs. stability, Properties: PH vs. Activity, pH vs. stability, Temperature vs. actividad: See following Temperature vs. actividad: See following graphics.graphics.

PHYTEX 500

PHYTEX 500

AGRANCO CORP USA

824 Coral Way, Coral Gables, Fl. 33134

Tel (305) 443 026 Fax (305) 443 7062

E mail: [email protected]

E. Mail: [email protected]

www.agranco.com

PHYTEX 500

agranco corp. usa

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