aisfnf0029 kind of session: poster p.6.01 session topic ...s.a.): control 2 (without essential...

P.6.01 - 1

Abstract Code: AISFNF0029 Kind of Session: Poster

Presentation:

Session: Session 6 Session Nr.: P.6.01 Session Topic: Nutrition and Health

Day: Thursday, June 7th Time: 10.30 - 11.15 Hall: Jerónimo Saavedra Hall

Number of authors: 1

AUTHOR 1: Presenting Author Dr. Lucia Elizabeth Cruz Suarez

Universidad Autonoma de Nuevo Leon

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P.6.01 - 2

Title:

“In vitro EVALUATION OF CRUDE EXTRACTS OF Bacillus pumilus, Bacillus safensis and

Pseudoalteromonas piscicida AGAINST SHRIMP PATHOGENIC BACTERIA AND THEIR POTENTIAL

ANTIBACTERIAL ACTIVITY”

Summary:

The shrimp farming industry has faced huge economic losses with bacterial diseases, like Vibriosis and acute

hepatopancreatic necrosis disease (AHPND). Marine bacteria were selected to evaluate the possibility for novel bioactive

compounds, as an alternative to control aquatic diseases. The aim of this study was to characterize the marine bacteria and

determine their antibacterial activity from crude extracts against bacterial species of aquaculture significance.

Three marine isolates were collected from the coast of Sonora, Mexico. The bacteria were characterized and identified (16S

rRNA) as Gram (+) spore forming rods, belonging to Bacillus pumilus (36R) and B. safensis (13L); and Gram (–) rods related to

Pseudoalteromonas piscicida (36Y). In vitro antagonism were evaluated by cross streak and double layer with AHPND

pathogenic V. parahaemolyticus strains MC32, B25, E14V2; and ATCC 17802. For the crude extract evaluation we used the

shrimp pathogens Pseudomonas sp. IST1, Pseudomonas sp. IST2, Bacillus sp. BGDGIIT3, Bacillus sp. BGDGNA1, Pseudomonas

sp. SPR2 T1, Vibrio fischeri strain HI; and MTCC cultures Vibrio parahemolyticus MTCC451, Vibrio alginolyticus MTCC4439,

Aeromonas hydrophila MTCC1739, Vibrio alcaligenes MTCC4442. For the production of antibacterial compounds the isolates

were grown in Zobell Marine broth, the biomass was separated and the cell free supernatant was extracted with ethyl

acetate (1:1, v/v). The organic phase was separated and concentrated using rotary evaporator; the sample was resuspended

in methanol. Methanol was used as control (Sruthy et al. 2014). The antibacterial activity against pathogens was confirmed

through Kirby-Bauer well diffusion method. Minimum Inhibitory Concentration (MIC) of extracts was determined in

tetrazolium microplate assay (Perumal et al. 2011). Biofilm disruption assay was observed by coverslip assay (Shumi et al.

2014) and by microtiter plate method (O’Toole, 2011). The active fractions (silica gel G) of the crude extract were separated

using thin layer chromatography (hexane:dichloromethane, ratio 5:5) for optimizing the suitable solvent system to be used

up for High Performance Liquid Chromatography (HPLC) analysis.

The ethyl acetate extract were found to be most active against almost all the bacterial species tested. This study promises an

interesting development of antibacterial compounds from B. pumilus, B. safensis and P. piscicida.

P.6.02 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Ayodeji Adeoye

Department of Aquaculture & Fisheries Management, Federal University of Agriculture, Abeokuta

- Nigeria

AUTHOR 2: Presenting Author Dr. Temilade Agbebi

(1)

AUTHOR 3: Prof. Simon Davies

Department of Animal Production, Welfare and Veterinary Sciences, Harper Adams University, UK

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P.6.02 - 2

Title:

“”

Summary:

A study was carried out to evaluate the effects of mannaoligosaccharides on the production of African catfish (Clarias

-1) were fed diet supplemented with 0% mannaoligosaccharides

(control diet) or diet supplemented with 0.02% mannaoligosaccharides (treatment diet). After 56 days of ad libitum feeding,

- of

catfish fed the experimental diets. Similarly, somatic indices (k-

different among the catfish fed either diet supplemented with 0% or 0.02% mannaoligosaccharides. However, further studies

are in progress to further determine the effects of mannaoligosaccharides on intestinal health (gut morphology and

microbiota profile) of catfish fed the experimental diets

P.6.03 - 1


Presentation:




AUTHOR 1: Mr. Thomas Mock

The Nutrition and Seafood Laboratory (NuSea.Lab), School of Life and Environmental Sciences,

Deakin University

AUTHOR 2: Presenting Author Mr. David Francis

The Nutrition and Seafood Laboratory (NuSea.Lab), School of Life and Environmental Sciences,

Deakin University

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P.6.03 - 2

Title:

“SYSTEMATIC REVIEW AND META-ANALYSIS HIGHLIGHT A PAUCITY OF PUBLISHED INFORMATION

REGARDING SPECIFIC DIETARY INFLUENCES ON OMEGA-3 FATTY ACID LEVELS IN FILLET TISSUE OF

ATLANTIC SALMON”

Summary:

Fillet fatty acid composition strongly reflects dietary fatty acid composition, yet published information quantifying the key

predictors of nutritionally valuable omega-3 fatty acids in commercially important finfish species is sparse. A systematic

review of published literature and meta-analysis quantified the major predictors of long-chain omega-3 polyunsaturated fatty

acid levels (n-3 LC PUFA) in the fillet of Atlantic salmon. Despite broad research interest in Atlantic salmon, there were

relatively few long-term growth trials conducted that reported the minimum dataset required to be of use in a meta-analysis

framework. From the limited data set available, principal component analysis and multiple regression techniques largely

supported previous research in isolating dietary fatty acids which have the dominant influence in determining levels of fillet

n-3 LC PUFA. Ultimately, the principal finding of the study points to the paucity of published studies implementing long-term

growth trials focussing on Atlantic salmon nutrition. Although long-term nutritional growth data is routinely acquired through

industry-based trials, much of this work remains ‘in-house’ and is not re-produced for publication in academic journals,

therefore, an enhanced focus on industry-academic collaboration is required. Furthermore, the published literature was

often excluded from subsequent analysis due to key statistical information not being reported such as a complete set of

means and standard deviations or standard errors.

P.6.04 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Meiling Zhang

School of life Sciences, East China Normal University

AUTHOR 2: Ms. Yi Sheng

School of life Sciences, East China Normal University

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P.6.04 - 2

Title:

“How High-fat Diet Regulates the Lipid Accumulation in Fish by Intestinal Microbiota”

Summary:

Abstract: It has been found that high-fat diet influenced the intestinal microbiota composition to regulate the lipid

metabolism in mammals. However, the interaction among the high-fat diet, intestinal microbiota and host metabolism in fish

remains unclear. In this study, Nile Tilapia were fed with diet containing soybean oil at the concentration of 7% and 13%,

respectively. The results indicated that soybean oil with the concentration at 13% increased the crude lipid significantly and

accordingly, the abundance of Enterobacteriaceae increased significantly. In order to identify the role of the bacteria in host

metabolism, one strain SOB9 which is affiliated to Enterobacteriaceae was isolated from the gut of Nile Tilapia and then

added to the diet to feed Nile Tilapia with the concentration at 105CFU/mL for 8 weeks. The results indicated that addition of

SOB9 increased the accumulation of abdominal adipose tissue, TG content in liver and the expression level of DGAT2 in the

liver. Intestinal structure was impaired in SOB9 added group and oral fat tolerance text (OFTT) experiment also indicated that

SOB9 addition increased the TG concentration in serum. This study suggested that high-fat diet increased the abundance of

Enterobacteriaceae which may impair the gut permeability, and increase the lipid accumulation in fish.

P.6.05 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. Ignasi Sanahuja

Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of

Barcelona

AUTHOR 2: Dr. Laura Fernández-Alacid

(1)

AUTHOR 3: Mr. Sergio Sánchez-Nuño

(1)

AUTHOR 4: Mr. Borja Ordóñez-Grande

(1)

AUTHOR 5: Dr. Enric Gisbert

IRTA, Centre de Sant Carles de la Ràpita, Unitat de Cultius Experimentals

AUTHOR 6: Dr. Antoni Ibarz

(1)

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P.6.05 - 2

Title:

“Analyzing epidermal mucus metabolites as non-invasive methodology on gilthead sea bream welfare:

effects of dietary additives”

Summary:

Several metabolites, mainly from plasma, have been usually used as indicators of fish status (nutritional, metabolic,

pathological…). However, blood extraction implies several factors that could add an extra-stress response in themselves. In

the last years, some studies suggested the collection of exuded mucus from skin surfaces as non-invasive approach in fishes,

reporting the presence of mucus components related to metabolism, environmental influences and nutritional status. The

present study aims to determine the effects of dietary additives, proposed on the DietaPlus project, on gilthead sea bream

(Sparus aurata) juveniles at mucus metabolites level.

Dietary trials were conducted at indoor facilities of the IRTA-San Carles de la Ràpita, Spain. In the first trial, five groups by

quadruplicate of sea bream juveniles were fed for 105 days with the same diet supplied with the following additives: Control

1 (non-additives), Immune 1 and Immune 2 (with a +0.15% and +0.30%, respectively, from Immunotec®, Tecnovit S.A. This

additive is composed of Echinacea pupurea, manano-oligosacharides, β-glucan and zinc sulphate), Yeast 1 and Yeast 2 (+1.1%

and 2.2%, respectively, of Debaromyces hansenii, CIBNOR, Mexico). In the second trial, three groups by quadruplicate of sea

bream juveniles were fed for 50 days with the same diet supplied with essential oils (confidential formulation from Tecnovit

S.A.): Control 2 (without essential oils), EO 1 (with garlic oil) and EO 2 (mixture of garlic oil with carvacrol and timol). At the

end of growth periods, morphometric parameters and mucus samples were obtained from both trials.

Mucus analyses from Trial 1 demonstrated that whereas lower inclusion of yeast beneficed fish growth (87.2 vs 78.1 g) un-

altering mucus metabolites, a 2.2% yeast inclusion would modify fish condition increasing glucose and lactate exudation,

suggested as indicators of fish stress. In addition, we can observe how the Immune 2 diet supposed lower levels of mucus

cortisol. From Trial 2, both diets reduced cortisol exudation to values practically undetectable with respect to the Control diet

2, a first indication of their preventive potential. Data should be analyzed in the context of the broad project DietaPlus where

they are included together with plasma, tissues and defenses studies.

P.6.06 - 1


Presentation:




AUTHOR 1: Presenting Author Ms. Alyssa MacDonald

University of Hawai'i at Manoa

AUTHOR 2: Mr. Alyssa MacDonald

University of Hawai'i at Manoa and Leeward Community College

AUTHOR 3: Mr. Rajesh Jha

University of Hawai'i at Manoa

AUTHOR 4: Ms. Kiesha Rednour

Leeward Community College

AUTHOR 5: Mr. Jordan Yoshioka

Leeward Community College

AUTHOR 6: Mr.

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P.6.06 - 2

Title:

“EVALUATION OF CASSAVA CHIPS AS AN ALTERNATIVE FEED INGREDIENT IN HYBRID TILAPIA

(OREOCHROMIS NILOTICUS x OREOCHROMIS MOSSAMBICUS) AQUACULTURE FROM GUT HEALTH

PERSPECTIVE”

Summary:

In order to increase aquaculture production while reducing production costs, alternative ingredients must be investigated to

reduce the dependency on conventional energy sources in aquaculture feeds. Like prebiotics, feed ingredients with high fiber

and resistant starch also modulate the intestinal microbiota of the host while providing nutritional value for growth

performance. Tilapia (Oreochromis sp.) are the most widespread aquaculture species in the world due to their relative

fecundity, omnivorous feeding habits, and tolerance of marginal growing conditions, making them ideal study species for

alternative feed ingredients. This study investigated the use of sun-dried cassava (Manihot esculenta) chips as corn

replacement to reduce feed cost while maintaining production levels of tilapia (O. niloticus × O. mossambicus). Four hundred

tilapia fingerlings (~10g initial body weight) were randomly and equally placed in 20 tanks and fed with one of the 5 diets

with 0, 4.38, 8.75, 17.5, and 26.25% cassava inclusion in basal diets for 12 weeks. Feed intake and body weight was recorded

weekly and intestinal and environmental samples were collected monthly and processed for microbiota analysis using PCR-

DGGE, metagenomics and qPCR. Tilapia growth performance was not significantly different among treatments (P>0.05),

suggesting that cassava can be incorporated up to 26.25% into the tilapia diets without negative impact. Additionally, cassava

inclusion into the tilapia diets significantly altered the intestinal microbiota, which can be used as a strategy to modulate gut

health of Tilapia.

P.6.07 - 1


Presentation:




AUTHOR 1: Presenting Author Prof. Lin Feng

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

AUTHOR 2: Prof. Xiao-Qiu Zhou

(1)

AUTHOR 3: Dr. Yu-Wen Dong

(1)

AUTHOR 4: Prof. Wei-Dan Jiang

(1)

AUTHOR 5: Prof. Pei Wu

(1)

AUTHOR 6: Prof. Yang Liu

(1)

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P.6.07 - 2

Title:

“Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-

κB and TOR signaling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with

Aeromonas hydrophila”

Summary:

The intestinal immunity is critical for intestinal healthy and animal welfare. Threonine is an essential amino acid for animals.

However, there are just fragmentary reports about the effects of threonine on immune responses in animal intestine. Thus,

this study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass

carp. Six iso-nitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine kg-1) were

formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 days. Results showed that

compared with optimum threonine supplementation, threonine deficiency: (1) decreased fish ability against enteritis,

intestinal lysozyme activities (LA) [except in the distal intestine (DI)], acid phosphatase (ACP) activities, complement 3 (C3)

and C4 contents, IgM contents [except in the proximal intestine (PI)], down-regulated the transcript abundances of liver

expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, hepcidin, IgZ, IgM and β-defensin1 (except in the PI) (P < 0.05); (2) could

up-regulate intestinal pro-inflammatory cytokines TNF-α, IL-1β, IL-6, IL-8, IL-17D mRNA levels partly relate to nuclear factor

kappa B (NF-κB) signaling; (3) could down-regulate intestinal anti-inflammatory cytokines transforming growth factor (TGF)-

β1, TGF-β2, IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly through target of rapamycin (TOR) signaling. These data

indicate that threonine could enhance fish intestinal immune response partly by increases of the contents or activities of

immune compounds as well as inhibition of excess inflammation related to inhibition of NF-κB signaling and activation of TOR

signaling. Finally, based on the specific growth rate (SGR), against the enteritis morbidity and IgM contents, the optimum

threonine requirements were estimated to be 14.53 g threonine kg-1 diet (4.48 g threonine 100 g-1 protein), 15.05 g

threonine kg-1 diet (4.64 g threonine 100 g-1 protein) and 15.17 g threonine kg-1 diet (4.68 g threonine 100 g-1 protein),

respectively.

P.6.08 - 1


Presentation:




AUTHOR 1: Prof. Åshild Krogdahl

Norwegian University of Life Sciences Faculty of Veterinary Science

AUTHOR 2: Dr. Trond Kortner

(1)

AUTHOR 3: Mr. Erling Olav Koppang

(1)

AUTHOR 4: Mr. Håvard Bjørgen

(1)

AUTHOR 5: Dr. Paul Midtlyng

Aquamedic AS(1)

AUTHOR 6: Dr. Elvis Chikwati

(1)

AUTHOR 7: Dr. Gerd Marit Berge

NOFIMA

AUTHOR 8: Dr. Aleksei Krasnov

NOFIMA

AUTHOR 9: Dr. Øystein Sæle

Institute of Marine Research

AUTHOR 10: Prof. Søren Balling Engelsen

University of Copenhagen

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P.6.08 - 2

Title:

“GutMatters – A new project: “Defining and improving intestinal health in farmed salmon in Norway””

Summary:

Gut inflammation, diarrhea, and lipid malabsorption are conditions frequently observed in salmon in Norway. Sporadically

also gut cancer is observed, all indicating suboptimal gut health. In light of the its importance for production and welfare, gut

health has gained little attention by fish farmers and their veterinary consultants. Previous research indicates that increasing

inclusion of plant and other alternative feed ingredients are important contributing factors to gut malfunction. Plants contain

antinutrients known to affect gut function and health, alter gut microbiota and reduce feed utilization. The overall goal of

this new project, a cooperation between the institutions indicated in the author list and advisors from the industry, is to

improve gut health in the salmon populations by strengthening knowledge on:

• Incidence of gut problems in commercial populations in Norway

• Importance of feed composition, environment, and production conditions for the presence and severity of the gut

health problems

• Possibilities for reducing gut health problems by alteration in feed composition.

The project comprises two main work packages: WP1 Field investigation of gut health in salmon in Norway. Fish samples will

be taken three times over one year after sea transfer from the same six populations spread along the coast of Norway, i.e.

two in the South, Mid and North Norway. Analyses will show status of gut structure, immune status, digestive and barrier

functions, and gut microbiota. Detailed analyses of feed samples, i.e. nutrient profile, fibre classes, and metabolome profile,

allow estimation of correlations between feed composition, environmental conditions, management, gut microbiota, gut

function and health. WP2 Controlled experiments. The work will combine feeding experiments with fish in sea water tanks as

well as model experiments with isolated gut sacs, followed by use of a package of analytical tools revealing effects on gut

structure, function, health and gut microbiota. The results will reveal interactions between plant feed ingredients, gut

function and health. The project was initiated in October 2017. The first results are indicating clear variation in gut health

between the farms. The presentation will contain results from the first round of field samplings, analyses of diet composition,

gut function and Health.

P.6.09 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. Jie Wang *(see 7 other authors in documnet)

Faculty of veterinary medicine, Norwegian University of life sciences

AUTHOR 2: Ms. Louise Buttle

Cargill Aqua Nutrition

AUTHOR 3: Mr. Olai Einen

Cermaq Group AS

AUTHOR 4: Mr. Jie Wang

Norwegian University of Life Sciences (NMBU)

AUTHOR 5: Mr. Trond Kortner


AUTHOR 6: Mr. Yanxian Li


AUTHOR 7: Mr. Alexander Torres




AUTHOR 9: Mr. Øyvind Brevik

Cermaq Group AS

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P.6.09 - 2

Title:

“Effects of life stages, smolt types (S0 and S1) and a functional feed on performance and gut health of

Atlantic salmon (Salmo salar) under arctic conditions”

Summary:

A consequence of the foreseen increase in demand for Atlantic salmon is search for new production sites. In Norway,

production increase is possible in the North. However, present knowledge on the requirement, regarding nutrition, disease

challenges as well as optimal management conditions, of Atlantic salmon under arctic conditions is limited. It is highly likely

that, due to environmental effects, e.g. day length and temperature on the fish biology, impact of diet composition on

disease resistance may differ from that of fish produced in southerly areas and that optimal production can only be obtained

with dietary supplements tailor made for fish in arctic areas.

The present study aimed at strengthening the knowledge on effects of dietary inclusion of functional ingredients on two

smolt types reared under arctic conditions to secure gut health and the best possible production efficiency.

Throughout the observation period, the fish were fed diets based on the same basal composition varying in nutrient density

according to the life stage of fish, either without or with supplementary functional ingredients. The diets were fed to two

types of smolt, Spring smolt (S1) and Autumn smolt (S0), throughout the production cycle from fresh to salt water stages

under arctic conditions. Tissue and gut content form proximal, middle and distal intestine were sampled from 6 fish per cage

during the growth period from fresh to salt water at 4 sampling times. Gut health and digestive functions were evaluated by

various endpoints measurements including biochemical and histomorphological tools, host gene expression and microbial

community signatures.

The results are under evaluation. Preliminary results of molecular analyses show clear differences regarding gut function

between life cycle stages, reflecting the ontogeny of the salmon digestive system. Only minor differences in gene expression

were seen between the diets. Immune related genes showed significant differences between the smolt types. Interactions of

smolt type and life cycle stage were found in most of functional classes of genes.

Keywords: Salmo salar, arctic condition, functional feed, smolt type, performance, gut health

P.6.10 - 1


Presentation:




AUTHOR 1: Presenting Author Ms. Mette Hofossæter

Norwegian University of Life Sciences (NMBU), Faculty of Veterinary Medicine, Oslo, Norway

AUTHOR 2: Dr. Randi Sørby

Norwegian University of Life Sciences, Faculty of Veterinary Medicine, Oslo, Norway(1)

AUTHOR 3: Dr. Felipe Reveco

Norwegian University of Life Sciences (NMBU), Department of Department of Animal and

Aquacultural Sciences, Aas, Norway

AUTHOR 4: Prof. Charles McLean Press

(1)

AUTHOR 5: Prof. Margareth Øverland

Norwegian University of Life Sciences (NMBU), Department of Department of Animal and

Aquacultural Sciences, Aas, Norway

AUTHOR 6:

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P.6.10 - 2

Title:

“Intestinal health of Atlantic salmon fed yeast produced from non-food biomasses”

Summary:

The Norwegian aquaculture industry is rapidly expanding which has increased the demand for ingredients for fish feed

production. During the last decades salmon feed has changed from being mainly based on marine ingredients such as

fishmeal to being increasingly based on plant ingredients. An important part of Foods of Norway's tasks is to develop novel

protein feed ingredients from national resources to contribute to a sustainable development of the Norwegian aquaculture

industry.

Yeast is a potentially high-quality protein source for Atlantic salmon, which additionally contains bioactive components (e.g.

beta glucan, manno-proteins, nucleic acids) that may have immunomodulating effects. Candida utilis yeast is a protein source

that has demonstrated positive health effects. As plant-based protein sources may impair intestinal health, it is important to

ensure that the introduction of novel protein in salmon feed does not compromise gut homeostasis.

The effect of yeast on the intestinal health of salmon was investigated by conducting small-scale feeding experiments where

salmon were fed a fishmeal-based negative control diet, a yeast-based test diet, or test diets containing soybean meal

combined with either 2.5% or 20% yeast. A soybean meal-based diet was used as positive control to induce soybean meal

induced enteritis (SBMIE). The morphology of the distal intestine was evaluated by histology. Length and area of simple

intestinal folds were measured and the immunohistochemical presence of T-cell populations (CD3- and CD8-positive cells)

was determined using image analysis.

Histological investigation of the distal intestine showed that salmon fed yeast had normal morphology similar to that of

salmon fed fishmeal. Additionally, there were no statistically significant changes in T-cell populations or morphometric

measurements between fish fed the yeast or fishmeal-based diet. Salmon fed soybean meal, either alone or in combination

with yeast, showed morphologic changes related to SBMIE. In general, there was an increase in T-cell populations in salmon

fed soybean meal. However, the number of CD8-positive cells was lower in salmon fed soybean meal in combination with

yeast compared with salmon fed soybean meal alone.

Yeast is a high-quality protein source with immunomodulating effects in the gut of Atlantic salmon.

P.6.11 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Trond M. Kortner

Faculty of Veterinary Medicine, Norwegian University of Life Sciences

AUTHOR 2: Dr. Elvis Chikwati

(1)

AUTHOR 3: Dr. Kai K. Lie


AUTHOR 4: Dr. Øystein Sæle


AUTHOR 5: Dr. Katerina Kousoulaki

Nofima AS

AUTHOR 6: Dr. Ingrid Lein

Nofima AS


Faculty of Veterinary Medicine, Norwegian University of Life Sciences Oslo Norway

AUTHOR 8:

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P.6.11 - 2

Title:

“Gut health and digestive function in the cleaner fish Ballan wrasse (Labrus bergylta)”

Summary:

Cleaner fish, such as the Ballan wrasse (Labrus bergylta), are widely used as a non-medicinal agent against sea lice in salmon

farms. For the salmon industry, there is now high focus on becoming self-sufficient in cultivated cleaner fish. Important steps

towards production of efficient and healthy Ballan wrasse have been taken, but there are still many challenges in the

production related to diet formulation, gut health and gut function. A better understanding of the basic biology of digestive

functions is vital to facilitate and optimize diet formulation and feeding regime to improve the overall production.

We will present data from a 4-week feeding trial in which Ballan wrasse (initial mean weight 75g) was fed either a reference

diet, or the identical diet supplemented with i) the antinutrient soya saponin (0.4%) ii) a commercial prebiotic (Aquate™ SG,

0.4%) or iii) a combination of soya saponin and prebiotics. Blood, tissue and gut content from four separate intestinal

segments were sampled from 6 fish per duplicate tank. Gut health and digestive functions were evaluated by various

endpoint measurements including biochemical and histomorphological tools and global gene expression profiling.

Only minor differences in fish growth were seen between the four dietary groups, but saponin, both alone and in

combination with prebiotics, increased gut indices. Blood markers were by and large not affected by diet, but saponin, both

alone and in combination with prebiotics, decreased blood plasma cholesterol. Dry matter of intestinal content and activity

levels of digestive enzymes were not affected by diet. Histomorphological analyses revealed clear structural alterations in the

gut of fish fed saponin, both alone and in combination with prebiotics. The results indicated a progressing inflammation with

increased infiltration by immune cells particularly into the distal parts of the intestine. Gene expression profiles obtained by

RNA sequencing mirrored the histological and biochemical changes induced by the saponin load. The work has provided

novel basic knowledge on the anatomy, digestive and immune function of the Ballan wrasse intestine. Additionally, the study

demonstrated that Ballan wrasse gut health and digestive function may be markedly affected by diet composition.

P.6.12 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Ayodeji Adeoye

Department of Aquaculture & Fisheries Management, Federal University of Agriculture, Abeokuta

- Nigeria

AUTHOR 2: Dr. Femi Fawole

2Department of Fisheries & Wildlife Management, College of Agriculture, Osun State University,

Osogbo - Nigeria

AUTHOR 3: Prof. Sam Obasa

(1)

AUTHOR 4:

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P.6.12 - 2

Title:

“Effect of yeast (Saccharomyces cerevisiae) products on African catfish (Clarias gariepinus) production”

Summary:

A study was carried out to evaluate the effects of yeast products (unextracted and hydrolysed yeasts) on growth of African

-1) were fed diets supplemented with either 0%

yeast product, 3% unextracted yeast or 0.3% hydrolysed yeast. After 56 days of ad libitum feeding, there was no significant

rate

- ntal diets.

Similarly, the somatic indices (K-

and haematological parameters were not significantly different (P > 0.05) among catfish fed the experimental diets. It could

be inferred that under optimum rearing condition yeast products (at current level of inclusion) do not enhance the growth

performance nor haematological parameters of C. gariepinus. Histological analysis of gut morphology as well as

microbiological analysis of gut microbiota profile are ongoing.

P.6.13 - 1


Presentation:




AUTHOR 1: Presenting Author Ms. Marta Carvalho

Grupo de Investigación en Acuicultura (GIA), Instituto Universitario Ecoaqua, Universidad de Las

Palmas de Gran Canaria

AUTHOR 2: Dr. Daniel Montero

(1)

AUTHOR 3: Dr. Manuel Gesto

Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research

Centre

AUTHOR 4: Ms. Ana Lencina

(1)

AUTHOR 5: Dr. Ivar Lund

Technical University of Denmark, DTU Aqua, Section for Aquaculture, The North Sea Research

Centre

AUTHOR 6: Dr. Marisol Izquierdo

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.13 - 2

Title:

“EFFECT OF DIFFERENT DIETARY N-3 LONG-CHAIN POLYUNSATURATED FATTY ACIDS LEVELS ON STRESS

RESPONSE OF MEAGRE (ARGYROSOMUS REGIUS, ASSO 1801) JUVENILES”

Summary:

This study aimed to determine the effect of different dietary levels of n-3 LC-PUFA on stress response of juveniles of meagre,

a novel species for European Aquaculture.

Fish of 2.8±0.23g IBW were fed 5 experimental diets with increasing n-3 LC-PUFA levels (0.8, 1.4, 2.0, 2.6 and 3.6% DM)

during 90 days. The largest growth was obtained in fish fed 1.4-2.6% LC-PUFA. At day 66th of feeding, juveniles fed diets 0.8,

1.4 and 2.6% were allocated in 9 tanks of 200L (density of 10 fish tank-1) and exposed to an episodic water level reduction. At

day 90, juveniles fed diets 1.4-3.6%, were distributed in 18 tanks of 500L tanks (density of 6 fish tank-1), and were confined in

2 cages (3 fish cage-1) during 7 days.

In the first stress challenge, there were no significant differences in basal cortisol levels, which were only slightly increased

after 1h of stress irrespective of the diet. However, 1h after stress, fish fed 1.4% n-3 LC-PUFA showed lower plasma glucose

levels as well as lower plasma lactate. Additionally, fish showed maximum levels of 5-HIAA 1h after stress. The 5-HIAA/5-HT

ratio increased significantly 1h after stress in meagre fed 0.8 and 1.4% diets whereas in meagre fed 2.6% n-3 LC-PUFA this

ratio was unaffected.

In the second challenge, plasma cortisol levels showed a global increase 2h after stress, with fish fed 1.4 and 2.0% n-3 LC-

PUFA showing the highest cortisol levels, and recovering to basal levels after 7 days. Regarding cat gene expression, fish fed

1.4% presented the highest expression 2h after confinement. After 7 days of prolonged stress, fish fed 2.0% diet showed

lower cat expression than fish fed 3.6%. Additionally, a global increase in gpx expression was observed 2h of confinement.

After 7 days, fish fed 2.0% and 2.6% diets presented significant lower gpx expression then fish fed 3.6%.

The results of this study confirmed growth and metabolic responses of previous studies that suggested a requirement of

about 2.0% DM n-3 LC-PUFA for this species, and denote that this level is also required to improve resistance to acute and

prolonged stress in meagre juveniles.

P.6.14 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Cristina Elena Trenzado

Universidad de Granada

AUTHOR 2: Dr. Amalia Pérez-Jimenez

Universidad de Granada(1)

AUTHOR 3: Dr. Julio José Trenzado

(1)

AUTHOR 4: Dr. Amalia Encarnación Morales

(1)

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.14 - 2

Title:

“Can dietary vitamins modulate kidney antioxidant status of rainbow trout under high rearing

conditions?”

Summary:

Five experimental diets were formulated according to different lipid sources (soybean and fish oil respectively; HUFA 12.5

and 30.5 g/kg diet respectively) and two levels of vitamin E (25.6 and 275.6 mg/kg diet) and vitamin C (0 and 1000 mg/kg

diet).

Rainbow trout (Oncorynchus mykiss) were maintained under high (100 kg/m3) and low (20 kg/m3) fish density for 42 days. A

total of 10 experimental groups (2 density conditions x 5 experimental diets) were assayed in triplicate. Antioxidant activity

was evaluated in kidney tissue. The assays manifested a marked effect of dietary composition on superoxide dismutase (SOD)

and catalase (CAT) activity of fish under high density conditions. Catalase was also affected in fish under low density

conditions. The results indicate that dietary vitamin E deficiency could promote some antioxidant enzymes activity to cover

an increased production of reactive oxygen species (ROS), being these effect more evident in fish under a compromised

condition such crowding. This response joined to a particular SOD isoenzyme pattern in these fish kidney, displaying the

presence of a Mn-SOD isoform (unpublished results) would reflect the influence of dietary composition modulating stress

response and mitochondrial involvement in the antioxidant status.

P.6.15 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. naheed bano

g.c women university sialkot, pakistan

AUTHOR 2: Dr. naheed bano

g.c women university sialkot, pakistan

AUTHOR 3: Dr. sadia maalik

govt. college women university sialkot, pakistan

AUTHOR 4: Dr. sajida mushtaq

g.c. women university sialkot, pakistan

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.15 - 2

Title:

“Effects of vitamin E supplementation in the diet of Rohu”

Summary:

The effect of dietary vitamin E supplementation in practical diet of Labeo rohita fingerlings was

evaluated in this study. The study was conducted in feeding trial of 60 days. At the onset of feeding trial, twenty-five fish

were stocked randomly into triplicate tanks (initial body weight 4.42±0.05 g). Results of feeding trial showed that weight

gain% and SGR of fish fed fresh fish oil and low oil oxidation level were significantly higher than those of fish fed high oil

oxidation. Among high oxidized oil containing treatments, fish feeding on 1000 mg/kg vitamin E showed better growth

performance compared to no or 100 mg/kg vitamin E. Oxidized oil ingestion significantly increased the moisture content and

decreased the protein content, while vitamin E supplementation reduced the fat content and increased the moisture content

in fish body. Oil oxidation caused a significant reduction in the concentrations of vitamin E in fish liver and muscles.

Increasing the vitamin E in the diet increased the α-tocopherol content in liver while muscle α-tocopherol content remained

un-influenced from dietary vitamin E level. Oxidized oil ingestion caused higher TBARS levels and antioxidant enzyme

activities in body, liver and muscles while dietary vitamin E supplementation abrogated these effects. The hepatic and

muscular PUFA, in particular, DHA was significantly increased by the inclusion of dietary oxidized oil, while vitamin E

supplementation in rancid diets suppressed DHA concentration to the level close to those from fresh oil diets. In conclusion,

the dietary vitamin E supplementation enhanced the growth performance and antioxidant status of fish. However, high doses

of vitamin E showed the pro-oxidative effect which promoted lipid peroxidation, leading to reduced growth rate. Higher

dietary lipid levels increased the growth rate, PUFA concentrations and lipid concentration in fish body than fish fed an

adequate lipid diet. Increase in the lipid supplementation level increased the need of vitamin E supplementation to achieve

adequate protection against oxidative stress. In short, vitamin E supplementation reduced the lipid peroxidation and

enhanced the growth performance and meet quality of L. rohita fingerlings irrespective of the level and quality of fish oil,

however, the required level of vitamin

P.6.16 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Amalia Pérez-Jiménez

University of Granada

AUTHOR 2: Dr. Carolina Castro

Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR)

AUTHOR 3: Dr. Cristina Trenzado


AUTHOR 4: Mr. Tiago Aires

Sorgal, Sociedade de Óleos e Rações S.A.

AUTHOR 5: Prof. Aires Oliva-Teles

University of Porto

AUTHOR 6: Dr. Helena Peres


AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.16 - 2

Title:

“Effect on haematological parameters and plasma metabolites of dietary tryptophan supplementation in

Dicentrarchus labrax reared at two density conditions”

Summary:

Fish farming faces numerous stress situations which may negativelly affect production, but the use of feed additives could

minimize the adverse effects. Tryptophan is an essential amino acid, responsible for serotonin and melatonin synthesis, both

of them neurotransmitters with very important biological functions, including stress alleviation. A study aimed to evaluate

the effect of different dietary tryptophan levels (0.42, 1.06, 2.12 and 4.25% (dry matter), corresponding to control, 2.5T, 5T

and 10T, respectively) in haematological parameters and plasma metabolites of sea bass (Dicentrarchus labrax, initial body

weight 8.0 g) was performed. Fish were reared at two densities (LD: 4 g/L and HD: 8 g/L) and kept in 60 L tanks supplied with

filtered seawater. Fish were hand-fed twice daily, 6 days a week, until visual satiation, during 6 weeks. During this time,

salinity averaged 35‰, dissolved oxygen was kept near saturation and water temperature 25±0.5°C. Results presented in

Table 1, show that only haematocrit, MCHC and lactate were affected by density conditions. So, at lower density lactate

values were increased (P<0.05) whereas haematocrit was significantly decreased. MCHC was lower at high density but only

for fish group fed on 10T diet. Regarding dietary tryptophan levels effects, the diet with the highest tryptophan content (10T)

induced a significant decrease of haematocrit, haemoglobin and plasma lactate values. On the contrary, MCHC was increased

in 10T group, but only in fish rearing at low density condition. Plasma glucose was not affected by any condition, either

density or dietary tryptophan level.

P.6.17 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Amalia Pérez-Jiménez


AUTHOR 2: Dr. Carolina Castro


AUTHOR 3: Dr. Eva E. Rufino-Palomares


AUTHOR 4: Ms. Elisabete Matos

Sorgal, Sociedade de Óleos e Rações S.A.


University of Porto



AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.17 - 2

Title:

“Effect on growth performance and feed utilization of dietary tryptophan supplementation in

Dicentrarchus labrax reared at two density conditions”

Summary:

The use of feed additives to minimize stress consequences and so, to optimize fish growth, is being widely studied in

aquaculture. Tryptophan is an essential amino acid for protein accretion, besides being responsible for important

neurotransmitters synthesis that have relevant biological functions, namely in stress mitigation. The present study aimed to

evaluate the effect of different dietary tryptophan levels (0.42, 1.06, 2.12 and 4.25% (dry matter), corresponding to control,

2.5T, 5T and 10T, respectively) in growth performance, feed efficiency, condition factor and survival of sea bass

(Dicentrarchus labrax, initial body weight 8.0 g) rearing at two densities (LD: 4 g/L and HD: 8 g/L). Fish were kept in 60 L tanks

supplied with filtered seawater. Fish were hand-fed twice daily, 6 days a week, until visual satiation, during 6 weeks. During

this time, salinity averaged 35‰, dissolved oxygen was kept near saturation and water temperature 25±0.5°C. Results

showed that density conditions did not influence any determined parameter except feed intake which was lower at higher

density conditions as shown in Table 1. On the contrary, different dietary tryptophan inclusion levels affected all evaluated

indexes (P<0.05), except survival. In general, at higher dietary tryptophan inclusion levels, lower values for growth

performance, feed intake, feed efficiency and condition factor, with the lowest results showed in fish group fed on 10T diet.

Fish fed on 2.5T diet did not show significant differences regarding control diet in any growth performance, feed utilization

and biometric index parameter. So, to determine the potential beneficial effect of dietary supplementation with tryptophan

requires to establish the optimum levels of this amino acid to be used for each fish species. In conclusion, levels up to 1.06%

of tryptophan can be used without negatively affect growth performance and feed efficiency of sea bass, whereas levels up

to 2.12% seem to be deleterious for this species, being even very negative when 4.25% level is used.

P.6.18 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Marcelino Herrera

IFAPA Centro Agua del Pino

AUTHOR 2: Ms. M. Antonia Herves

IFAPA Centro Agua del Pino(1)

AUTHOR 3: Mr. Juan L. Roca

(1)

AUTHOR 4: Dr. Emilio Morales

University of Huelva

AUTHOR 5: Dr. Inmaculada Giráldez

University of Huelva

AUTHOR 6: Mr. Marcelino Herrera

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.18 - 2

Title:

“Stress-attenuating diets with amino acid supplements do not alter the energy metabolism in meagre

(Argyrosomus regius)”

Summary:

It has been demonstrated that some dietary amino acids can attenuate the stress response in fish. Those amino acids are

usually incorporated to the fish feed as additives, and can unbalance the basal energy metabolism, especially that of proteins

and amino acids. In this work the effects of two experimental diets for mitigating the stress response on the energy

metabolism have been studied. One hundred and fifty meagres, (105 ± 2.6 g) were distributed in 6 tanks (25 fish/tank). Fish

from 2 tanks received a commercial diet (Control group), those from another tank received a diet supplemented with

aspartate (Asp group) and fish from the other 2 tanks received a diet supplemented with tryptophan (Trp group). Fish were

fed these diets during 7 days. Afterwards, 30 fish were sampled for basal values (10 per diet), and other 30 fish for post-

stress values 1 h after submitting them to air exposure for 3 min. Liver samples were taken for analysing enzyme activity of

energy metabolism: alanine transaminase, aspartate transaminase, fructose biphosphatase, phosphofructokinase and

pyruvate kinase. Although the efficacy of the amino acid supplements had already been confirmed, changes in enzymes

related to energy metabolism were not significant among treatments. Therefore the supplements assayed could be a suitable

option to improve the fish welfare without unbalancing the metabolism.

P.6.19 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Laura Fernández-Alacid

Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of

Barcelona

AUTHOR 2: Mr. Ignasi Sanahuja

(1)

AUTHOR 3: Mr. Borja Ordóñez-Grande

(1)

AUTHOR 4: Mr. Sergio Sánchez-Nuño

(1)

AUTHOR 5: Dr. Marcelino Herrera

IFAPA Centro Agua del Pino

AUTHOR 6: Dr. Antoni Ibarz

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.19 - 2

Title:

“Mucus metabolites to determine the netting stress in meagre: effects of preventive supplementation

with specific amino acids”

Summary:

In teleosts, the skin mucus is the first physical barrier against physical and chemical attacks. It contains components related

to metabolism, environmental influences and nutritional status. Recently, we showed that some mucus metabolites could be

non-invasive indicators of a distress condition in fishes. In this way, we conducted an indoor study in meagres, Argyrosomus

regius, which were stressed via air exposition or netting. Two amino acids, tryptophan and aspartate, involved in

neurotransmission and energy metabolism, were additionally supplied in order to test their possible role in the alleviation of

stress and, hence, improvement of fish welfare.

Six batches of meagre (105 ± 2.6 g) were kept in the facilities of IFAPA Centro Agua del Pino (Huelva), with a temperature of

19.0 ± 1.0 ºC. Fish were randomly distributed in the three experimental feeding conditions: Control (commercial diet), Asp

(control diet supplemented with 1% of aspartate) and Trp (supplemented with 1% of tryptophan). These experimental

cultures were carried on for a short-period of 7 days. Thereafter, two different types of stress were induced: Air exposure (3

min) or Netting (confinement and chasing/netting with no air exposure for 3 min, 6 times an hour). Samples from epidermal

mucus were obtained before stress (basal), and 1h and 6h post-stress. The mucus metabolites (glucose, lactate, protein) and

cortisol were analyzed from mucus soluble extracts.

First evidences from mucus analyses indicated that the stress response was also detectable at epidermal mucus level by the

increases of glucose, lactate and cortisol exudation, which should be derived from the internal response. However, air

exposure and netting stress resulted in different responses, being mucus glucose risen by air, and mucus protein decreased

by netting. Amino acid preventive supplementation for one week with Asp or Trp showed markedly differences; whereas Trp

addition seems to be protective to mitigate the netting stress, the Asp addition would enhance glucose exudation and energy

loss.

In conclusion, a pre-treatment with additional dietary tryptophan would mitigate the stress response in meagre to cope with

the fishing procedure, although further studies on this response under farming conditions should be necessaries

P.6.20 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Filipe Coutinho

CIMAR/CIIMAR – Interdisciplinary Centre Of Marine And Environmental Research, University of

Porto

AUTHOR 2: Ms. Marta Monteiro

Department of Biology, Faculty of Science, Porto University

AUTHOR 3: Dr. Claudia Serra

(1)

AUTHOR 4: Ms. Rafaela Santos

Department of Biology, Faculty of Science, Porto University


(1)


(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.20 - 2

Title:

“Methionine and aurine effects on post inflammation oxidative stress in european sea bass

(Dicentrarchus labrax) juveniles”

Summary:

This study aimed to evaluate the effects of dietary methionine (Met) and taurine (Tau) supplementation to low fish meal

(FM) diets on the immune response and post inflammation oxidative stress in European sea bass juveniles. For that purpose,

four practical diets were formulated to be isolipidic (18%) and isonitrogenous (6.7%), containing 82% of the protein from

plant origin. Two of those diets had a Met level 12% below (LMetLTau) or 15% above (HMetLTau) requirements, one of each

being supplemented with 1% Tau (LMetHTau and HMetHTau, respectively). Following a 12 weeks growth trial, fish were

subjected to an inflammatory challenge, being intraperitoneally injected with LPS (lipopolysaccharide from Photobacterium

damselae subsp. piscicida) or PBS (phosphate buffer saline), as control group. At 4 and 24 hours post-injection (hpi) head

kidneys were collected to measure the expression of several immune related genes by real-time PCR and intestines were

collected to measure antioxidant enzymes activities and lipid peroxidation. The expression of pro-inflammatory cytokines,

responsible for the acute-phase reaction, interleukin 1 beta (IL-1β), tumor necrosis factor (TNF-α) and interleukin 6 (IL-6),

increased quickly in response to LPS injection, being higher at 4 hpi than at 24 hpi. At 4 hpi the intestine oxidative status of

fish injected with LPS remained unaltered, while at 24 hpi superoxide dismutase activity significantly increased in the

intestine of fish injected with LPS. Moreover, fish fed LMetLTau diet also showed higher lipid peroxidation levels in the

intestine in response to LPS injection. However, HMetLTau diet decreased lipid peroxidation levels of the intestine of fish

injected with LPS. Additionally, some effects of dietary Met or Tau supplementation in the activity of antioxidant enzymes

that were observed in fish injected with PBS were not evident in LPS treatment. In conclusion, feeding European sea bass a

diet with low Met and Tau levels was shown to increase intestine oxidative stress 24 hpi with LPS, while dietary Met

supplementation reverts this condition.

P.6.21 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Yanjiao Zhang

Ocean University of China

AUTHOR 2: Mr. Zhichu Chen

(1)

AUTHOR 3: Ms. Sifan Zhao

(1)

AUTHOR 4: Ms. Yang Liu

(1)

AUTHOR 5: Ms. Pei Yang

(1)

AUTHOR 6: Prof. Kangsen Mai

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.21 - 2

Title:

“Dietary citric acid supplementation alleviates soybean meal induced intestinal oxidative damage and

micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L.”

Summary:

A 12- week feeding trial was conducted to investigate the protective effects of citric acid against soybean meal induced

intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (Scophthalmus maximus L.). Four

isonitrogenous and isolipidic experimental diets, i.e., fish meal-based diet (FM), FM with 40% fish meal protein replaced with

soybean meal protein diet (SBM), SBM supplemented with 1.5% citric acid diet and SBM with 3% citric acid diet respectively,

were designed. Triplicate groups of 30 fish were fed to apparent satiation twice daily. There is no obvious morphological

damage observed in fish fed FM and two citric acid diets, while the typical impairment induced by soybean meal induced was

observed in fish fed SBM. The activities of intestinal trypsin, lipase and amylase in citric acid groups were significantly higher

than these activities in SBM group. Dietary citric acid in SBM diet significantly enhanced the total anti-oxidative capacity and

the gene expression of superoxide dismutase, glutathione peroxidase and heme oxygenase 1, and decreased

malondialdehyde content in the distal intestine. Additionally, the genes expression of proliferating cell nuclear antigen

(PCNA), protein kinase C (PKC), mucin-2 and mucin-18 were remarkably elevated by dietary citric acid supplementation, while

the genes expression of p53, Caspase-3 and Caspase-7 were significantly declined. Sequencing of bacterial 16s rRNA V4

region showed that Proteobacteria and Firmicutes were the dominant intestinal bacteria of turbot in FM group. Compared to

the SBM group, dietary citric acid significantly reduced the relative abundance of Bacteroidetes, maintaining the domination

of Proteobacteria and Firmicutes. The similarity of intestinal microbial communities in citric acid group was closer to FM

group from PCoA and UPGMA analysis. MetaStat analysis indicated that dietary citric acid dramatically reduced the relative

abundance of the Vibrio genus. In brief, dietary citric acid regulated p53 and PKC signal transduction and thereby mitigate

soybean meal induced intestinal oxidative damage, beneficially modulated the micro-ecological balance and specifically

reduced the relative abundance of the Vibrio genus in the distal intestine of juvenile turbot.

P.6.22 - 1


Presentation:




AUTHOR 1: Ms. MARWA MECHLAOUI

University of Las Palmas de Gran Canaria

AUTHOR 2:

AUTHOR 3:

AUTHOR 4:

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.22 - 2

Title:

“EFFECTS OF DIFFERENT DIETARY SELENIUM SOURCES ON GROWTH PERFORMANCE, LIVER AND MUSCLE

COMPOSITION, ANTIOXIDANT STATUS, STRESS RESPONSE AND EXPRESSION OF RELATED GENES IN

GILTHEAD SEABREAM (SPARUS AURATA)”

Summary:

Selenium is an indispensable trace element for fish with different metabolic functions including protection against oxidative

stress. The present study aimed to determine the effect of dietary inclusion of either an inorganic or an organic selenium (Se)

source on gilthead sea bream, Sparus aurata. Triplicate groups of juvenile gilthead (IBW 6.16 ± 0.04g and IBL 7.65 ± 0.01cm)

were each fed one of five diets: basal diet with no added Se (negative control, 0.8 mg Se/kg diet), supplemented with 0.2 or

0.5 mg Se/kg as sodium selenite (NaSe) or with 0.2 and 0.5mg Se/kg as hydroxy-selenomethionine (OH-SeMet) providing (1,

1.3, 1.1, and 1.4 mg Se/kg diet respectively) for 11 weeks. After the feeding trial, fish were submitted to an acute stress by

fish capture and a chronic confinement stress. Blood samples were withdrawn at 0 and 2 h after the acute stress and after 7

days of chronic stress for cortisol analyses. Liver and muscle composition and Se content, malondialdehyde (MDA)

concentration in muscle and liver, expression of related hepatic genes, such as superoxide dismutase (sod), catalase (cat) or

glutathione peroxidase (gpx), were also determined.

Growth rate was the highest in fish fed OH-Se-Met at 0.2mg/kg although without significant difference with fish fed the

control diet and the lowest growth was observed in fish fed Na-Se 0.5. Increase in dietary Se, particularly in the form of OH-

SeMet, tend to increase the hepato-somatic index (HIS). Thus, HSI in OH-SeMet 0.5 fed fish was significantly higher than in

the control without Se supplementation, in agreement with a higher lipid content registered in these fish. Dietary inclusion of

OH-SeMet, led to a significant reduction in MDA in both liver and muscle. However, supplementation in the form of NaSe up

to 0.2 did not prevent peroxidation processes in any of these two tissues, in comparison with fish fed the control diet.

Dietary inclusion of Se at 0.2mg/kg significantly reduced plasma cortisol after acute stress, regardless of the Se form fed.

Moreover, a slight mortality occurred during the chronic stress challenge that affected mostly fish fed Na-Se 0.5, followed by

the control fish fed diets

P.6.23 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Seunghan Lee

University of Idaho, Hagerman Fish Culture Experiment Station

AUTHOR 2: Dr. Karthik Masagounder

Evonik Nutrition & Care GmbH

AUTHOR 3: Dr. Brian C. Small

(1)

AUTHOR 4: Dr. Biswamitra Patro

(1)

AUTHOR 5: Mr. Donald Sneddon

Nutrition & Care, Evonik Corporation

AUTHOR 6: Dr. Ronald W. Hardy

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.23 - 2

Title:

“Performance of rainbow trout (Oncorhynchus mykiss) fed diets varying in fishmeal and crude protein

levels exposed to normal-growing or chronic-stress conditions”

Summary:

Immense efforts have been made over the years to reduce dietary fishmeal (FM) and crude protein (CP) levels in order to

move the aquaculture industry more sustainable. Supplemental amino acids have been used as a strategy to balance the

diets for essential amino acids (EAA) while reducing expensive FM or CP levels. The objective of this study was to evaluate the

effects of reducing dietary FM and CP levels on the growth performance of rainbow trout under normal growing condition

and their physiological response under chronic stress condition.

Eight experimental diets (isocaloric) with 4×2 factorial design were formulated to contain two levels of FM (20%, 5%) and

four levels of CP (45%, 42%, 39%, 36%). Diets were supplemented with increasing levels of amino acids to balance the diets

for EAA. Trout (34.8±0.3g) were randomly stocked into triplicate groups of tanks at 30 fish per tank. Fish were fed to

apparent satiation twice daily for nine weeks to assess growth performance under laboratory rearing conditions, and then for

an additional six weeks with and without exposure to handling stress twice per week.

Reducing dietary FM levels from 20% to 5% significantly reduced fish growth and increased FCR (P<0.05) after 9 weeks of

feeding period. Reducing dietary CP levels from 45% to 39% did not affect trout growth or weight gain, however further

reduction in CP (36% vs. 42% or 45% CP) resulted in significant drop in fish growth. Reducing dietary CP levels to 36% did not

affect FCR (0.90±0.03). No interaction effect was detected between these two factors in fish growth or FCR. Exposure of fish

to stress increased plasma glucose and cortisol levels. There were, however, no significant differences in plasma lysozyme,

glucose and cortisol among dietary treatments after stress. Reducing FM and CP levels did not affect these indices of non-

specific immunity or the stress response. In conclusion, our study showed that reducing FM level to 5% affects performance,

while diets balanced for amino acids and other critical nutrients show an opportunity to reduce CP level from 45% to 39%

without any reduction in trout performance.

P.6.24 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. Thomas Clark

University of Aberdeen

AUTHOR 2: Prof. Samuel Martin


AUTHOR 3: Dr. Daniel Macqueen


AUTHOR 4: Dr. John Tinsely

Biomar

AUTHOR 5: Dr. Trygve Sigholt

Biomar

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.24 - 2

Title:

“Molecular characterization of enzyme families controlling the urea cycle and their transcriptional

responses to inflammation in rainbow trout (Oncorhynchus mykiss)”

Summary:

The full function and response of the urea cycle is still poorly characterised in teleost fish. The urea cycle is based around the

metabolism of arginine and its precursor amino acids ornithine and citrulline. In salmonids, arginine is an essential amino acid

as sufficient quantities cannot be synthesised endogenously and must be obtained in the diet. Arginine is also believed to be

essential in both the inflammatory innate immune response and subsequent tissue healing. To further understand the role of

the urea cycle and related cycles (polyamine synthesis and nitric oxide production) in teleosts, we characterised gene families

encoding the key enzymes in this pathway, and their expression during in vivo and in vitro inflammatory responses. Due to

two whole genome duplication events in salmonid evolutionary history, several genes in these pathways were discovered to

exist as paralogues, with divergent sequences and in some cases altered regulation. These genes could potentially be useful

markers for understanding the impacts of functional amino acid supplementation in relation to fish health.

P.6.25 - 1


Presentation:




AUTHOR 1: Mr. Shijun Chen

Guangdong Provincial Key Laboratory of Improved Variety Reproduction in Aquatic Economic

Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University

AUTHOR 2: Mr. Jin Niu



AUTHOR 3: Mr. Yongjian Liu



AUTHOR 4: Presenting Author Mr. Lixia Tian



AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.25 - 2

Title:

“Changes in antioxidant status, immune response and ammonia stress tolerance of juvenile Pacific white

shrimp (Litopenaeus vannamei) fed different levels of dietary myo-inositol”

Summary:

The purpose of the present study was to investigate the effects of dietary myo-inositol (MI) on the survival rate, immune

response and antioxidant status after ammonia stress in Litopenaeus vannamei. Six practical diets supplemented with graded

levels of MI (designated as MI0, MI100, MI200, MI400, MI 800 and MI1600, respectively) were fed to triplicate groups of L.

vannamei (mean weight 0.40 ± 0.00 g ) for 8 weeks. 180 shrimp from the six experimental groups (mean weight

approximately 11-14g) were exposed to ammonia stress for 24 h after feeding trial. Reduced survival after 24 h ammonia

stress was present in shrimp fed the MI-unsupplemented diet as compared to those fed MI100, MI200 and MI1600 diets.

Compared with the control group, after exposed to ammonia stress, the plasma of alkaline phosphatase and acid

phosphatase activities increased, however, opposite results were observed in hepatopancreas. The shrimp fed MI-

unsupplemented diet had lower activities of total antioxidant capacity, glutathione S-transferase and glutathione peroxidase

and content of reduced glutathione, and higher malondialdehyde and protein carbonyl content in plasma or hepatopancreas

compared with MI-supplemented groups. In addition, mRNA expression levels of the antioxidant and immune genes ferritin,

arginine kinase, thioredoxin, heat shock protein 70, catalase and peroxiredoxin were significantly differentially regulated in

hepatopancreas. Broken-line regression analysis on glutathione peroxidase activity and quadratic regression analysis on

reduced glutathione indicated that the dietary MI requirement of L. vannamei was 673.5-1207.5mg kg-1 diet. These results

suggested that dietary MI could improve immune response, antioxidant capacity and stress resistance after ammonia stress

in L. vannamei.

P.6.26 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Rita Azeredo

Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto

AUTHOR 2: Ms. Marina Machado

(1)

AUTHOR 3: Dr. Sergio Fernández-Boo

(1)

AUTHOR 4: Dr. Luís Conceição

SPAROS Lda.

AUTHOR 5: Dr. Nuno dos Santos

i3S/IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto

AUTHOR 6: Dr. Benjamín Costas

(1)

AUTHOR 7: Ms. Filipa Fontinha

Instituto de Ciências Biomédicas

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.26 - 2

Title:

“Arginine or citrulline supplementation in diets for European seabass deteriorates host immune condition

and inflammatory response”

Summary:

The present study was designed to determine the modulatory effects of arginine and citrulline dietary supplementation on

the immune condition and inflammatory response of the European seabass, Dicentrarchus labrax. Four diets were

manufactured: a control diet (CTRL) was formulated to meet the indispensable amino acids profile established for seabass.

Based on this formulation, three other diets were supplemented with L-arginine at two different levels (0.5 % and 1 % of

feed, ARG1 and ARG2, respectively) and DL-Citrulline at 0.5 % (CIT). Fish were fed these diets for 2 or 4 weeks until visual

satiation under controlled conditions. Blood samples were withdrawn from the caudal vessel at the end of each feeding

period for the evaluation of the haematological profile. Blood smears were prepared for differential cell counting and total

peripheral leucocytes were counted as well. Blood was finally centrifuged and plasma samples were stored at -80 °C until

assayed. Head-kidney tissue was also collected for the assessment of immune-related gene expression and kept at -80 °C

until processed. At the same time, fish from all dietary treatments were intraperitoneally injected with Photobacterium

damselae subsp piscicida (100 µL, 5 x 103 CFU ml-1) and similarly sampled after 4, 24 or 48 h to follow the inflammatory

response. Plasma peroxidase activity, total white blood cells and lymphocytes decreased with time before bacterial challenge

regardless of dietary treatment, whereas lysozyme, total white blood cells, lymphocytes, monocytes and neutrophils

decreased in fish fed supplemented-diets after infection. Expression patterns of a panel of 25 genes are currently being

analysed and should further enlighten about immune mechanism in which arginine is involved. Nonetheless, results generally

point at an impairment of both the immune status and inflammatory response upon dietary supplementation of either amino

acid, a scenario that might result from nitric oxide toxic effects. Citrulline supplementation-induced changes were similar to

those inflicted by arginine, suggesting that under immune stimulation, citrulline to arginine conversion rate might increase

resulting in similar immune-impairment leading mechanisms.

P.6.27 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. PEDRO L. P. F. CARVALHO

UNESP, FMVZ - AquaNutri

AUTHOR 2: Mr. IGOR S. T. VICENTE

(1)

AUTHOR 3: Ms. HINGLIDJ C. MULLER

(1)

AUTHOR 4: Mr. WILSON M. FURUYA

(1)

AUTHOR 5: Prof. LUIZ E. PEZZATO

(1)

AUTHOR 6: Prof. MARGARIDA M. BARROS

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.27 - 2

Title:

“Dietary glutamine supplementation on innate immune response of Nile tilapia subjected to bacterial

challenge”

Summary:

Glutamine (Gln) is a major energy substrate for cells of the immune system, playing important roles in their function and

homeostasis. In mammals, Gln participates in lymphocyte proliferation, enhancement of phagocytic cell activity and bacterial

killing, immunoglobulin synthesis, cytokine production and T-cell responses. However, the role of this amino acid in fish

immune system is still poorly understood. Thus, a study was carried out to evaluate the effects of dietary Gln

supplementation on the innate immune system of Nile tilapia subjected to bacterial challenge by Aeromonas hydrophila.

Four hundred Nile tilapia juveniles (4.4 ± 0.1 g) were distributed in 40 aquaria (250 L each), in a recirculating water system.

The experimental design utilized was completely randomized with five treatments (0, 0.5, 1.0, 1.5 and 2.0 % Gln) and eight

replicates. After the 90-day feeding trial, serum lysozyme activity (LSZ), hydrogen peroxide (H2O2) and nitric oxide (NO) levels

were analyzed. Subsequently, 12 fish per treatment were inoculated intraperitoneally with the pathogen and transferred to

the experimental challenge room. After 15 days, the same immune parameters were analyzed. The bacterial challenge

affected H2O2 levels and LSZ activity (P < 0.05) but had no significant effects on NO. Glutamine supplementation had no

significant effect on H2O2 and NO concentration. Fish fed supplemented diets up to 1.5% Gln presented significantly higher

LSZ activity, but further supplementation did not elicit additional improvements according to the Tukey’s test (P > 0.05). In

conclusion, dietary Gln supplementation presented limited effects on Nile tilapia innate immune function.

P.6.28 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. Haokun LIU

Institute of Hydrobiology the Chinese Academy of Sciences

AUTHOR 2: Mr. Peiyu ZHANG

(1)

AUTHOR 3: Mr. Xiaoming ZHU

(1)

AUTHOR 4: Mr. Dong HAN

(1)

AUTHOR 5: Mr. Junyan JIN

(1)

AUTHOR 6: Mr. Shouqi Xie

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.28 - 2

Title:

“Effects of dietary inosine 5’monophosphate on growth performance, immune response, and salinity

tolerance of gibel carp”

Summary:

Inosinic acid or inosine monophosphate (IMP) is a nucleoside monophosphate. IMP is an intermediate ribonucleoside

monophosphate in purine metabolism. Important derivatives of inosinic acid include purine nucleotides found in nucleic

acids and adenosine triphosphate, which is used to store chemical energy in muscle and other tissues. To study the effects of

dietary IMP on growth performance, salinity tolerance, and immune response of gibel carp (Carassis auratus gibelio var. CAS

III), four isonitrogenous and isoenergetic semipurified diets (393±4 g crude protein and 19.2±0.3 MJ gross energy kg-1 dry

matter) were formulated containing four graded dietary IMP levels (0%, 0.1%, 0.2% and 0.4%). Each experimental diet was

randomly allocated to three tanks at a density of 30 fingerlings (average initial body weight 7.48±0.09g). Feeding trial lasted

for 3 months in recirculating water system. After feeding trial, fish were challenged with Aeromonas hydrophila by

intraperitoneal injection and exposed to12% NaCl solution. Results showed that daily feeding rate and plasma neuropeptide

Y decreased with increasing dietary IMP level and was significantly lower in 0.2% dietary IMP treatment than control group

(P<0.05). Statistically insignificant but higher weight gain and feed efficiency was observed in groups fed diet with increasing

dietary IMP level. After bacterial challenge test, cumulative survival rate, plasma myeloperoxidase and plasma superoxide

dismutase activity was significantly higher in 0.4% group than that of control group (P < 0.05). Plasma lysozyme activity in all

groups showed lower values after bacterial challenge and significant decline was found in control group (P < 0.05). Plasma

lysozyme activity in 0.2% and 0.4% IMP group was significantly lowered than that of control before challenge test (P < 0.05). 6

hour post salinity stress, plasma cortisol, kidney Hsp70 gene expression and kidney glucocorticoid receptor gene expression

was found to be significantly higher in control group than that of 0.4% IMP group (P < 0.05). While post salinity stress, plasma

glucose level and gill filament Na+-k+ ATPase activity increased and plasma potassium level decreased significantly (P < 0.05)

in 0.4% dietary IMP group. In conclusion, dietary IMP can improve the immunity and salinity tolerance of gibel carp.

P.6.29 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Albert Caballero-Solares

Department of Ocean Sciences, Memorial University

AUTHOR 2: Mr. Xi Xue


AUTHOR 3: Dr. Jennifer R. Hall

Aquatic Research Cluster, CREAIT Network, Memorial University

AUTHOR 4: Dr. Christopher C. Parrish


AUTHOR 5: Dr. Richard G. Taylor

Cargill Innovation Centre

AUTHOR 6: Dr. Matthew L. Rise


AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.29 - 2

Title:

“Progress towards a better understanding of the impact of diet on immunity of farmed Atlantic salmon

(Salmo salar)”

Summary:

The Biomarker Platform for Commercial Aquaculture Feed Development project has provided us with knowledge and tools

that will help design superior health-promoting diets for Atlantic salmon. The project investigated the immunomodulatory

effects of dietary formulations and lipid profiles that are alternative to those of traditional fish meal/fish oil-based diets. A

first study revealed that high replacement levels of fish meal/fish oil by soy protein concentrate and rapeseed oil in the diet

increased the head kidney immune-related transcriptomic response to a viral mimic (polyriboinosinic polyribocytidylic acid,

pIC). Based on diet composition, we hypothesized that dietary ARA/EPA ratios were key to such a modulatory effect. To a

limited extent, Atlantic salmon are capable of synthesizing ARA and EPA, which are involved in immune and inflammatory

processes through their derivative eicosanoids. In this regard, a second study showed that high dietary levels of EPA

precursor [α-linolenic acid, an omega-3 (ω3) FA] strengthened the induction of tlr7, irf7b, stat1b, rsad2, isg15a, and mxb by

pIC. In addition, high levels of ARA precursor (linoleic acid, an ω6 FA) were found to enhance the induction of pro-

inflammatory (il8, cox2) and antibacterial (camp) transcripts upon challenge with formalin-killed Aeromonas salmonicida

(ASAL). These findings are now being applied to the Integrated Pathogen Management of Co-infection in Atlantic Salmon

(IPMC) project. In the IPMC project, we have tested diets with three different ratios of ω3 and ω6 FAs (high ω3, high ω6, and

balanced ω3/ω6) in combination with three levels of DHA and EPA (0.3, 1.0, and 1.4%, of diet). The synergy of dietary high

ω6 FA levels and 0.3% DHA+EPA resulted in a higher induction of cox2 and camp by ASAL antigens. A stronger induction of

stlr5a by ASAL was observed in salmon fed elevated ω6 levels, regardless of the DHA+EPA levels. Conversely, high ω3 FA

levels combined with 0.3% DHA+EPA up-regulated the constitutive transcription of antiviral transcripts (tlr7 and irf7b).

Furthermore, independent of dietary DHA+EPA levels, salmon fed high ω3 FA levels showed increased pIC-induction of isg15a

and mxb. Taken together, our results help lay the foundations for an effective dietary manipulation of Atlantic salmon

immunity.

P.6.30 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Chris Parrish

Department of Ocean Sciences

AUTHOR 2: Mr. Tomer Katan

(1)

AUTHOR 3: Mr. Xi Xue

(1)

AUTHOR 4: Dr. Albert Caballero-Solares

(1)

AUTHOR 5: Dr. Richard Taylor

Cargill Innovation Center

AUTHOR 6: Dr. Matt Rise

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.30 - 2

Title:

“Growth, head kidney lipid composition, and gene expression in Salmo salar fed varying levels of

different ω3 and ω6 PUFA”

Summary:

In teleost fish the head kidney (HK) is important in hematopoiesis and immune responses and is the major antibody

producing organ. Given the connection between polyunsaturated fatty acids (PUFA) and inflammation and immunity we

investigated the effect of feeding diets varying between 2- and 8-fold in individual C18 - C22 PUFA on Atlantic salmon. A

feeding trial was conducted in which 275±54 g salmon were fed a commercial control diet and 7 experimental diets with low

EPA+DHA contents (0.3 – 1.4% of diet) and varying 18:2ω6 (LNA) and 18:3ω3 (ALA) levels to provide high ω3 or ω6 diets and

a medium ω3 and ω6 diet. After 12 weeks there were only small differences (3-14%) among the treatments in the 13 growth

performance variables measured; however, multivariate statistics revealed significant differences in overall growth

performance among the high ω3/1% EPA+DHA treatment, the medium ω3&ω6/1.4% EPA+DHA treatment, and the

commercial diet treatment. There were much larger differences in HK fatty acids which reflected dietary levels. HK ARA

varied 2½-fold reflecting a 3-fold variation in dietary LNA, and causing a 4-fold variation in HK ARA/EPA ratio. Dietary LNA

correlated with HK ARA and ω6:ω3 ratio, while ALA correlated with HK total ω3 levels. HK EPA proportions were maintained

at the same level when EPA+DHA contents were as low as 0.3% of diet, so long as high levels of ALA were provided. This is

consistent with gene expression data showing a significant up-regulation of HK transcripts involved in lipid synthesis. The

relative quantity (RQ) values of elovl5a (fatty acyl elongation) were positively correlated with both dietary and HK levels of

ALA. srebp1 (fatty acid and cholesterol synthesis) was positively correlated with HK ω3:ω6 fatty acid ratio. The diet with the

highest amount of ALA and low EPA+DHA led to the lowest RQ values for pro-inflammatory cox2 (eicosanoid synthesis). Thus,

while reduction of dietary EPA+DHA to low levels had little effect on growth, there were significant effects on HK gene

expression related to lipid synthesis depending on dietary ALA. Concomitantly there were large changes in HK ARA/EPA ratios

and gene expression related to inflammation.

P.6.31 - 1


Presentation:




AUTHOR 1: Prof. RINA CHAKRABARTI

UNIVERSITY OF DELHI

AUTHOR 2: Prof. JAIGOPAL SHARMA

DELHI TECHNOLOGICAL UNIVERSITY

AUTHOR 3: Mr. NEELESH RAJPUT

Delhi Technological University

AUTHOR 4:

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.31 - 2

Title:

“Evaluation of Immunostimulatory properties of Achyranthes aspera in rohu Labeo rohita in pond

conditions”

Summary:

The immunostimulatory and disease resistance properties of leaves and seeds of Achyranthes aspera were tested in rohu

Labeo rohita in pond conditions. Rohu fry were fed with two test diets containing 0.5% seeds and 0.5% leaves of Achyranthes

aspera and control diet. The average weight was significantly (P<0.05) higher in 0.5% seeds enriched diet fed rohu compared

to other two treatments on days-7, 14 and 21 after immunization of fish. Specific growth rate also showed the similar trend.

Food conversion ratio was significantly (P < 0.05) lower in 0.5% seeds supplemented diet fed rohu compared to other two

treatments. Serum lysozyme and myeloperoxidase activities were maximum in seeds supplemented diet fed rohu compared

to the others. Thiobarbituric acid reactive substances and carbonyl protein levels were significantly (P < 0.05) higher in the

control diet fed rohu compared to the fish fed with other diets. The expressions of various immune related genes were

recorded in the hepatopancreas of rohu. There were up regulations in the expression of lysozyme C, lysozyme G, anti-

inflammatory gene TNF-α, IL-10 and IL-1β in seeds and leaves supplemented diets fed rohu compared to the control diet fed

fish on various days of immunization.

P.6.32 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Jon Øvrum Hansen

Foods of Norway, Norwegian University of Life Sciences

AUTHOR 2: Dr. Leidy Lagos

(1)

AUTHOR 3: Mr. Peng Lei

(1)

AUTHOR 4: Dr. Felipe Eduardo Reveco-Urzua

(1)

AUTHOR 5: Dr. Liv Torunn Mydland

(1)

AUTHOR 6: Mr. Margareth Øverland

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.32 - 2

Title:

“Effect of down-stream processing of bakers yeast (Saccharomyces cerevisiae) on digestibility and

immune response in Atlantic salmon (Salmo salar)”

Summary:

The increased demand for high-quality feed ingredients for the aquaculture industry have given increased focus on microbial

ingredients as sources of both protein and lipids. However, limited information exist on the effect of different down-stream

processing of yeast on nutrient digestibility and immune response in fish. In the present study, we used three different down-

stream processing methods for yeast: spray drying, autolysis or cell crushing and evaluated their effects on nutritional and

health for Atlantic salmon (Salmo salar).

In detail, we first re-suspended fresh live bakers yeast (Saccharomyces cerevisiae) in deionized water and then

processed using one of the following six methods: spray dried at 1) 180 or 2) 250 °C, treated with a high shear processor

LM20 Microfluidizer with a force of 3) 10.000PSI or 4) 20.000PSI, and autolyzed using a 30 l Bioreactor system at 50 °C for 5)

8 or 6) 16 h. Nitrogen leakage and fixation of yeast cells for electron microscope were performed before final spray drying.

The experimental diets consisted of 700 g kg-1 of a fishmeal based reference diet ( including yttrium as a digestible

marker) and 300 g kg-1 of one of the processed yeast candidates. Fish were fed with one of the seven resulting diets for 30

days, and faeces, spleen, head kidney and plasma were collected from fish at the end of the experiment.

The results showed that dry matter (DM) digestibility ranged from 42.9 to 63.6% on ingredient level across the

experimental diets in which spray drying at 250 °C had the lowest digestible value while autolyzing yeast for 16 h yeast

presents the highest. Digestibility of protein ranged from 49.5 to 89.7% among treatments and followed same pattern as DM

digestibility. Fish growth rate and feed conversion ratio supported the difference in protein digestibility among the different

treatments. The expression of IgM in leukocytes was analyzed by flow cytometry and morphology and mucus from the distal

intestine was observed by confocal microscopy. In conclusion, different down-stream processing of yeast had an impact on

protein utilization and immune response when used as a protein source in fish feed.

P.6.33 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. José María García Beltrán

Fish Innate Immune System Group. Department of Cell Biology and Histology. Faculty of Biology,

Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia,

Murcia, Spain.

AUTHOR 2: Dr. Cristóbal Espinosa Ruiz

Università degli Studi di Palermo, Dipartimento di Scienze della terra e del Mare DiSTeM,

Laboratorio di Biochimica Marina ed Ecotossicologia, Trapani, Italy.

AUTHOR 3: Mr. Vittorio Campo

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of

Messina

AUTHOR 4: Dr. Latifeh Chupani

Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture

and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University

of South Bohemia in Ceske Budejovice, Czech Republic.

AUTHOR 5: Prof. Caterina Faggio

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of

Messina

AUTHOR 6: Prof. María Ángeles Esteban Abad

Fish Innate Immune System Group. Department of Cell Biology and Histology. Faculty of Biology,

Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia,

Murcia, Spain.

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

P.6.33 - 2

AUTHOR 12:

Title:

“Origanum vulgare administered in fish diets enhance humoral and cellular immunity of gilthead

seabream (Sparus aurata L.)”

Summary:

The use of antibiotics in farmed fish can have negative effects in fish, in the environment and also in consumers. For these

reasons, different plants have been considered as a friendly alternative to antibiotics. Origanum vulgare, distributed around

the Mediterranean area, is the most important and most variable specie of genus of medicinal plants Origanum, and due the

climate and land, this plant is very common in Spain. O. vulgare present many beneficial properties such as antimicrobial,

anti-stress and immunostimulant. In the current study, the effects of dietary supplementation with O. vulgare were studied

on the growth, immune and anti-oxidant status of gilthead seabream (Sparus aurata) over a period of 30 days. The

experimental diets were enriched with 0 (control), 0.5% and 1% of O. vulgare. Results demonstrate that the supplemented

diet had no effect neither on growth nor on the anti-oxidant enzymes activity, although an increased tendency in the total

antioxidant activity in serum and skin mucus were observed after 30 days of feeding with 0.5% or 1% diets in comparison

with control group. Fish fed diets enriched with O. vulgare for 15 and 30 days had an improved immune status. Respect

humoral immunity, 1% diet enhanced in skin mucus IgM level at 30 days and bactericidal activity against Photobacterium

dampselae at 15 and 30 days. Furthermore, fish fed 0.5% and 1% enriched diets had increased serum protease activity

compared to control fish after 30 days. Regarding cellular immunity, head kidney leucocytes phagocytic ability was also

enhanced in fish fed 0.5% enriched diet for 15 days. In conclusion, O. vulgare could be considered as a good dietary

immunostimulant supplement to be included in gilthead seabream diet.

P.6.34 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Femi Fawole

Department of Aquaculture and Fisheries, University of Ilorin, Kwara State, Nigeria

AUTHOR 2: Dr. Narottam Sahu

Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries

Education, Mumbai, India.

AUTHOR 3: Ms. Shamna N

Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries

Education, Mumbai, India.

AUTHOR 4: Dr. Ayodeji Adeoye

Department of Aquaculture and Fisheries Management, Federal University of Agriculture,

Abeokuta, Nigeria

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.34 - 2

Title:

“Immuno-biochemical response and hepatic oxidative status of Labeo rohita fingerlings fed detoxified

Jatropha Protein Isolate”

Summary:

The growing demand for fish feed and the high cost of conventional feed ingredient has prompted researchers to seek for

alternative feed ingredient especially plant materials to meet the need for protein resources in the aquafeed sector. In

response to this urgent need, the present study was conducted to evaluate the nutritional potential of detoxified Jatropha

protein isolate (DJP) in animal feeding and its effect on the immuno-biochemical response and hepatic oxidative status of

Labeo rohita fingerlings. Two hundred and sixteen fingerlings (initial average weight 3.25 ± 0.02 g) were randomly distributed

into 18 rectangular tubs, each containing 12 fish, and fed with six isonitrogenous (311.3 g/kg) and isocaloric (17.12 MJ/kg)

diets for 60 days. Soybean protein isolate (SPI) served as the major protein source in the control diet and was progressively

replaced at 25%, 50%, 75% or 100% with DJP, and additional 100% replacement group supplemented with L-Lysine

monohydrochloride. Fish were fed to satiation with their respective diets twice daily at 10:00 h and 18:00 h. The results

showed that all the haematological parameters except white blood cell counts showed no significant differences (P > 0.05)

compared with the control. The immuno-biochemical parameters such as total protein, albumin, globulin, total

immunoglobulin, respiratory burst and lysozyme activities did not differ significantly (P>0.05) among the various groups.

Furthermore, the hepatic oxidative stress enzyme like superoxide dismutase (SOD), catalase (CAT), and oxidative stress

marker malondialdehyde (MDA) showed no remarkable changes (P > 0.05) compared with the control group. The results

obtained in the current study revealed that the replacement of SPI with DJP in the diet of L. rohita fingerlings did not cause

any oxidative induced stress nor alter the immuno-biochemical responses of the fed fish. Hence, DJP may be suggested as a

replacement for SPI in aquafeed manufacturing.

Keywords: Jatropha protein, haematology, innate immunity, oxidative enzyme, Labeo rohita

P.6.35 - 1


Presentation:




AUTHOR 1: Presenting Author Ms. Thi Mai Nguyen

Author

AUTHOR 2: Ms. Thi Nang Thu Tran

Second author

AUTHOR 3: Mr. Syaghalirwa N.M. Mandiki

3th author

AUTHOR 4: Mr. Yvan Larondelle

4th author

AUTHOR 5: Mr. Patrick Kestemont

Correspondence

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.35 - 2

Title:

“Beneficial combination of β–glucan with different dietary lipid sources on growth, immune response,

fatty acid profile and expression of several genes involved in immunology, lipid biosynthesis and

eicosanoid process in common carp (Cyprinus carpio)”

Summary:

In recent years, the influence of nutrition on fish immunology is of increasing interest; however, data on carp (Cyprinus

carpio) are still limited. This study aims to evaluate the effects of a combination of β–glucan with dietary lipid sources on the

immune response, fatty acid profile and their interactions in carp juveniles (16.3±0.6g BW). Six isoproteic (39.1%) and

isolipidic (10%) diets containing different lipid sources (cod liver oil – CLO, linseed oil – LO and sunflower oil – SFO) were

formulated with (CLO+, LO+, SFO+) or without (CLO, LO, SFO) β–glucan supplementation. Fish were fed a daily ration of 4%

body weight during 9 weeks and then challenged with Aeromonas hydrophyla for 10 days. At the end of the experiment and

second day of the challenge test, plasma was sampled for lysozyme and complement activity assays; liver and muscle were

used for fatty acid analyses; kidney and liver were served for gene expression analyses. No significant differences of final

body weight, growth rate, daily weight gain, feed conversion rate and survival rate (P>0.05) were observed. We found an

influence of lipid source and β–glucan supplementation on lysozyme activity (P<0.05) but no effects were observed on

complement activity neither at the end of the growth trial, nor after the challenge test. We also found a diminution of these

immune parameters in the challenge test compared with those at the end of the growth trial (P<0.05). Fatty acid profiles in

fish liver and muscle reflected those of the diets and the difference was observed in all studied criteria (P<0.05). Cod liver oil

was the best dietary lipid source in terms of essential fatty acid content and n3/n6 ratio (P<0.05) in fish tissues, but linseed oil

appeared as a suitable alternative plant oil in carp. The expression of several genes involved in immune function (nk, lyz, il-8),

lipid biosynthesis (elovl5, fads2) and eicosanoid process (pla2a2, ara1) respectively was also investigated but did not display

any difference (P>0.05). In conclusion, the supplementation of β-glucan at the dose of 0.25g.kg diet-1 increased the lyzozyme

activity but not the growth and chemical composition in carp

P.6.36 - 1


Presentation:




AUTHOR 1: Mr. Francisco Guardiola

Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), University of Porto

AUTHOR 2: Presenting Author Mr. José Maria García-Beltran

Department of Cell Biology and Histology, University of Murcia

AUTHOR 3: Ms. María Ángeles Esteban

Department of Cell Biology and Histology, University of Murcia

AUTHOR 4:

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.36 - 2

Title:

“Effects of dietary administration of fenugreek seeds on metabolic parameters and immune status of

gilthead seabream (Sparus aurata L.)”

Summary:

Medical plants could be used as a prophylactic method in aquaculture because they are considered safe and so very

promising alternatives to the use of chemicals. The aim of the present work was to examine the effects of dietary fenugreek

(Trigonella foenum graecum) seeds administered for 8 weeks on the metabolic and cellular immune status as well as, on the

expression levels of some immune-related genes in head-kidney of gilthead seabream (Sparus aurata L.). Four experimental

groups were designated: one receiving a basal diet (control) and three fed powdered fenugreek seeds incorporated in the

fish feed at 1 %, 5 % and 10 %. The results show that significant decreases in aspartate aminotransferase, creatine kinase,

potassium and the albumin/globulin ratio were detected in the serum of fish fed 10% fenugreek compared with the values

recorded in control fish. Interestingly, the results revealed a significant enhancement of most of the cellular immune

parameters studied, especially in fish fed the highest level of fenugreek (10 %). Regarding to gene expression, dietary

supplementation down-regulated the expression of hep and hsp70 genes in fish fed the lowest inclusion of fenugreek (1%

and 5%). Contrarily, no significant variations were observed in HK gene expression of fish fed the highest rate of fenugreek

(10%) after 8 weeks of feeding. Overall, the results suggest that the high level of dietary fenugreek tested in this work (10 %)

did not negatively affect any of the metabolic parameters measured in serum but increased some of them. In addition, the

inclusion of fenugreek seeds in the gilthead seabream diet at 10 % improved the cellular immune activities and it not affects

negatively the expression of immune-related genes.. Further studies are needed to better understand the effects of this

natural product, which may be suitable for use as a feed additive in fish aquaculture.

P.6.37 - 1


Presentation:




AUTHOR 1: Presenting Author Prof. Wei-Dan Jiang

Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

AUTHOR 2: Prof. Xiao-Qiu Zhou

(1)

AUTHOR 3: Prof. Lin Feng

(1)

AUTHOR 4: Prof. Pei Wu

(1)

AUTHOR 5: Prof. Yang Liu

(1)

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.37 - 2

Title:

“Aflatoxin B1 damages the structural integrity of immune organs as well as the potential mechanisms in

fish”

Summary:

The head kidney and spleen are the main immune organs of fish and their structural integrity are crucial for fish healthy.

Aflatoxin B1 (AFB1) is one of the most prevalent and toxic in nature mycotoxins. However, AFB1 toxic effects on structural

integrity of immune organs as well as potential mechanisms has not been clarified systematically in fish. In this study, a total

of 1440 juvenile grass carp were fed six diets containing graded levels of AFB1 0 (not-added group), 30, 60, 90, 120 and 150

μg/kg (determined level was 0.04, 29.48, 58.66, 85.94, 110.43 and 146.92 μg/kg diet) for 60 days. The results indicated that

dietary AFB1 caused poor growth performance and damage of immune organs structural integrity in fish. To explore the

underlying mechanisms of this toxicity, we for the first time demonstrated that it is partly referring to (1) induction of

oxidative damage and attenuation of antioxidant ability through up-regulation of Keap1a (not Keap1b) to suppress Nrf2

signaling leading to decrease of mRNA levels and activities of antioxidant enzymes (except GSTP1 mRNA level); (2)

aggravation of apoptosis partly by activating p38 MAPK signaling to activate mitochondria pathway and death receptor

pathway; (3) weakening TJs by promoting MLCK signaling to down-regulate the mRNA of TJs protein ZO-1, ZO-2b, occludin,

claudin-b, -c, -7a, -7b, -11 and -15a mRNA levels (expect claudin-12 was up-regulated), with no effect on claudin-3c mRNA

level in the head kidney and spleen of juvenile grass carp. In addition, AFB1 only down-regulated occludin mRNA level in the

head kidney rather than in the spleen. Together, these results indicate that AFB1 could damage the structural integrity of

immune organs referring to oxidative damage, apoptosis and the TJs disruption. Finally, based on the growth performance

(PWG and FE) and oxidative injury related indexes (contents of the ROS and MDA in the head kidney and spleen), the safe

dose of AFB1 for grass carp were estimated to be 29.48 μg/kg diet.

P.6.38 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Shiwei Xie


Animals, Institute of aquatic economic animals, school of life sciences, Sun Yat-sen University

AUTHOR 2: Mr. Jin Niu


Animals, Institute of aquatic economic animals, school of life sciences, Sun Yat-sen University(1)

AUTHOR 3: Mr. Weiping Fang



AUTHOR 4: Mr. Fan Yang



AUTHOR 5: Mr. Yongjian Liu

(1)

AUTHOR 6: Mr. Lixia Tian

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.38 - 2

Title:

“Dietary replacement of fish-meal impaired immune capacity, induced intestinal damage and changed

the member phospholipid composition of juvenile Pacific white shrimp, Litopenaeus vannamei at low

salinity”

Summary:

An 8-week feeding trial was conducted to evaluate the effect of fish-meal replacement on growth performance, protein

synthesis and immune ability of juvenile Pacific white shrimp, Litopenaeus vannamei at low salinity. Five practical diets were

formulated to contain graded levels (25, 20, 15, 10 and 5%) of fish-meal. Crystalline amino acids, phytase, mannan

oligosaccharides and some micro-nutrients were supplemented in the low fish-meal diets. Weight gain and survival were

decreased with the decreasing fish meal levels. When dietary fish-meal decreased, glucose, cholesterol and triglyceride in

hemolymph decreased. The gene expression of TOR, Raptor and eIF4E2 were decreased with the decreasing fish meal levels,

while 4E-BP was increased with the decreasing fish meal levels. ER stress related genes expression in liver and intestine were

significantly influenced by fish meal levels. NF-κB pathway also was affected by fish meal levels. HE stain and TEM analysis of

intestinal samples indicated that low fish meal diets induced intestinal damage of white shrimp. UPLC-Q/TOF-MS was used to

detect the metabolites in hemolymph of shrimp, DHA and EPA related phospholipids were significantly influenced by fish

meal levels, the change of member composition may responsible for the changed immune response of shrimp. The results

indicated that weight gain of white shrimp were decreased when fish-meal levels decreased to 10%, ER stress and intestinal

damage were induced when fish meal level was decreased to 15%, the suppressed immune response of shrimp fed low fish

meal diet may induced by the change of member phospholipids compositon.

P.6.39 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Cristina Elena Trenzado

Universidad de Granada

AUTHOR 2: Dr. Ramón Carmona

Universidad de Granada(1)

AUTHOR 3: Dr. Amalia Pérez-Jiménez

(1)

AUTHOR 4: Mr. Julio José Trenzado

(1)

AUTHOR 5: Dr. Amalia Encarnación Morales

(1)

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.39 - 2

Title:

“Influence of dietary lipid source and culture density on liver histology and antioxidant status of rainbow

trout.”

Summary:

Rainbow trout (Oncorhynchus mykiss) were maintained under high (100 kg/m3) and low (20 kg/m3) fish density conditions

for 42 days. During the experimental time, fish were fed on five diets that were formulated considering two different levels

of vitamin E (25.6 and 275.6 mg/kg diet), vitamin C (0 and 1000 mg/kg diet) and HUFA (12.5 and 30.5 g/kg diet): -E-HUFA, -

E+HUFA, +E-HUFA, +E+HUFA, -C+E+HUFA. Two lipid sources were used: fish oil for +HUFA diets and soybean oil for -HUFA

diets. A total of 10 experimental groups (2 density conditions x 5 experimental diets) were assayed in triplicate. Antioxidant

capacity was evaluated in liver. Specific activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX),

glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PDH) and lipid peroxidation level (malondialdehyde

concentration, MDA) were assayed. Tissue livers were sampled for histological study. Antioxidant enzymes revealed a

marked effect of dietary composition compared to fish density. Antioxidant vitamins deficiency (particularly vitamin E)

induced a significant increase of SOD, CAT, GR activities possibly in response to an enhanced production of reactive oxygen

species (ROS). Lipid peroxidation concentration presented no remarkable changes during the experimental period among

treatments, showing that the antioxidant response was effective to prevent oxidative damage. -E+HUFA treatment induced

histological differences in liver parenchyma with hepatocytes displaying increased vesicles of small size in hepatocites

compared to the remaining experimental groups.

P.6.40 - 1


Presentation:




AUTHOR 1: Presenting Author Prof. CHUNFANG CAI

SOOCHOW UNIVERSITY

AUTHOR 2: Ms. SHENJIE REN

(1)

AUTHOR 3: Mr. ZHI WANG

(1)

AUTHOR 4: Mr. SHIBIN YAO

(1)

AUTHOR 5: Mr. GUANGTONG CUI

(1)

AUTHOR 6: Prof. YUANTU YE

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.40 - 2

Title:

“Pectin interferenced the enterohepatic circulation and biosynthesis of bile acid in Pelteobagrus

fulvidraco and induced hepatic adipose infiltration”

Summary:

Non-starch polysaccharide reaches 20-40% in most vegetable feed stuff, its physiological significance to fish, however, did

not be paid enough attention. Pectin is a type of non-starch polysaccharide, which is widely exists in plant ingredients. In this

paper, we studied the effect of pectin on serum biochemical indexes, enterohepatic circulation and biosynthesis of bile acid,

and liver histology. 30% pectin was included in diet and named as pectin diet. Two other diets were formulated by supplied

pectinase and bile acid respectively into pectin diet. Fish meal based diet was used as control. Pelteobagrus fulvidraco (initial

body weight of 5.73 + 0.5g) were fed for 8 weeks with 3 replicates. Fish was sampled after fasting for 24 hours. The serum

triglyceride in all group of fish fed diet containing pectin was significantly decreased (P<0.05). Compare with the control

group, the total bile acid (TBA) and total cholesterol (TC) in the liver of fish fed pectin diet were significantly decreased.

Typical steatosis and fibrosis was noticed in liver tissue of fish fed diet pectin, and the liver lipid content was significantly

higher than that in fish fed control diet (P<0.05), which is mild in fish fed diet contain pectinase and bile acids. The intestinal

villi was fell off, disintegrated and the muscle membrane was separated in fish fed pectin diet. Study on differentially

expressed genes in liver between fish fed control diet and pectin diet based on transcriptomes with high-throughput RNA-seq

technology, significantly down regulated expression (P<0.05) was noticed in genes involved in steroid biosynthetic pathway

in fish fed diet pectin, while the gene expression involved in immune deficiency were significantly up-regulated (P<0.05).

These results first suggested that that pectin reduce the absorption and synthesis of bile acid in pelteobagrus fulvidraco, and

thereby disturb the absorption and turnover of lipid, and impact on immuninity.

P.6.41 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. Petter Gjesdal

Norwegian Institute of Marine Research

AUTHOR 2: Dr. Nina Liland


AUTHOR 3: Dr. Ikram Belghit


AUTHOR 4: Dr. Erik-Jan Lock


AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.41 - 2

Title:

“Assessment of liver lipid accumulation in Atlantic salmon (Salmo salar) fed an insect-based diet”

Summary:

Increased production of salmon has forced the aquaculture industry to look at alternative ingredients for feed production.

Insects are part of a natural diet for salmon and a potential ingredient in future feed. In this study, the fish meal (FM) of a

control diet was replaced completely with insect meal (IM) made from black soldier fly (Hermetia illucens) larvae (BSFL). The

fish were fed the experimental feeds (IM-0 and IM-100) during 16 weeks in the sea water phase.

Cryosectioned liver (n = 18) was stained with oil red o to visualize hepatic lipid accumulation. Image analyses tools were used

to determine the number and size of lipid droplets in the hepatic sections. Additionally, lipid class analysis was performed to

look for changes in the amount of storage fat in the liver. Results from these methods, including a discussion about the

results will be added later as this study is still in process.

P.6.42 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. M.A. Ruiz

Aquaculture Research Group (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria,

PCTM

AUTHOR 2: Prof. M.B. Betnacor

Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling.

AUTHOR 3: Prof. C.M. Hernández-Cruz


PCTM

AUTHOR 4: Prof. D. Montero


PCTM

AUTHOR 5: Dr. G. Rosenlund

Skretting Aquaculture Research Centre.

AUTHOR 6: Mr. M.S. Izquierdo


PCTM

AUTHOR 7: Mr. R. Fontanillas

Skretting Aquaculture Research Centre

AUTHOR 8: Dr. M.J. Caballero


PCTM

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.42 - 2

Title:

“The effect of fish density and dietary supplementation of vitamin C, manganese, zinc and selenium on

the development of systemic granulomatosis in juvenile meagre (Argyrosomus regius)”

Summary:

Systematic granulomatosis is a chronic disease that affects the majority of farmed meagre (Argyrosomus regius) and has

been proposed that has a nutritional origin due to the impossibility of identify any type of infectious agent in fish with

granulomas. Little is known about the nutritional requirements of meagre, indeed a dietary imbalance of some vitamins or

minerals could lead to metabolic disorders. In order to try to elucidate the nutritional origin of granulomas, juvenile meagre

were fed five isolipidic (16.7%) and isoproteic (49.6%) fish meal and fish oil-based feeds prepared by adding different levels of

vitamin C, minerals and vitamin E and K (100 and 35 mg.kg-1, respectively): Diet KEC (100 mg.kg-1C), Diet KEC+Mn/Zn/Se

(100 mg.kg-1C, 40 mg.kg-1Mn, 200 mg.kg-1Zn, 1.5 mg.kg-1Se), Diet KECC (600 mg.kg-1C), Diet KECCC (1.200 mg.kg-1C), Diet

KECCCC (3.200 mg.kg-1C). All diets were tested with 100 fish/tank (3,20 kg.m-3), but diet KECC and KECCCC was also tested at

a higher density (175 fish/tank; 6,20 kg.m-3). Growth performance (final weight, SGR, FCR and K factor) was only affected by

stocking density, being lower at high density. Percentage of fish with granulomas in any tissue was significantly lower in fish

fed diets KECCC and KECCCC at low density (80 and 76%, respectively), compared with fish fed all the other diets (87-90%). A

lower number of fish with hepatic granulomas was found in fish fed KECCC and KECCCC at low density as well as fish fed

KECCCC in high density. TBARS content was correlated with the percentage of granulomas in liver (R2=0.9439, y=0.003x-

0.1242). In addition, fish fed KECCCC showed the highest expression of cat in liver and kidney as well as the highest

expression of sod and gpx in liver and heart. The present results show that high levels of vitamin C (1.200-3.200 mg.kg-1C)

and low stock density (3,20 kg.m-3) favours the growth of juvenile meagre, reducing the lipid peroxidation indicators and

decreasing the incidence of granulomas, which confirms that this pathology is mostly triggered by deficiency of antioxidant

nutrients.

P.6.43 - 1


Presentation:




AUTHOR 1: Presenting Author Mr. M.A. Ruiz


PCTM.

AUTHOR 2: Prof. C.M. Hernández-Cruz


PCTM.

AUTHOR 3: Prof. M.J. Caballero


PCTM.

AUTHOR 4: Dr. Saleh R.

AAquaculture Research Group (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran

Canaria, PCTM.

AUTHOR 5: Prof. Izquierdo M.S.


PCTM.

AUTHOR 6: Prof. Betancor M.B.

Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling

AUTHOR 7: Dr. H. Fernández-Palacios


PCTM.

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.43 - 2

Title:

“Incidence of systemic granulomatosis is modulated by the feeding sequence, type of enrichment and

dietary supplementation of vitamin E and C in meagre (Argyrosomus regius) larvae”

Summary:

One of the most critical issues in meagre (Argyrosomus regius) production is the fish health, considering the systemic

granulomatosis as the most frequent disease in this species. Systemic granulomatosis has been studied in juvenile meagre,

but there is not any study regarding the first appearance of granulomas in larvae. Two experiments were carried out to

determine the early appearance of granulomas and its association with feeding sequences and vitamin levels during the

larval stage. In trial 1, larvae were fed four different feeding sequences (3-30dph): RS (Rotifer enriched with Easy DHA Selco

3-30dph), RO (Rotifer enriched with Ori-Green 3-30dph), AS (Rotifer (3-21dph) and Artemia (12-30dph) enriched with Easy

DHA Selco) and AO (Rotifer (3-21dph) and Artemia (12-30dph) enriched with Ori-Green), a co-feeding period with a

commercial microdiet (20-31dph) was carried out in all diets. Microscopic first appearance of granulomas was observed at

20dph in fish fed diet RS and RO (1.3-2% of larvae with granulomas, respectively). At 30dph granulomas were significantly

higher in fish fed RS and RO (10.7-15.3%) compared with fish fed AS and AO (0-0.7%), regardless of the enrichment. TBARS

values were significantly higher in fish fed RS and RO and were highly correlated with the incidence of granulomatosis

(R2=0.948, y=0.084x-4.3924). In trial 2, larvae were fed RS (3-30dph) and subsequently five different microdiets (30-44dph):

C- (40 mg.kg-1E, 100 mg.kg-1C), C+ (400 mg.kg-1E, 1.000 mg.kg-1C), Krill (400 mg.kg-1E, 1.000 mg.kg-1C and substitution of

fish oil by krill oil), EC (200 mg.kg-1E, 500 mg.kg-1C) and EECC (800 mg.kg-1E, 2.000 mg.kg-1C). Larvae growth performance

was not affected by any dietary treatment, although granulomas were observed in fish fed all diets, except in larvae fed high

levels of vitamin E and C (EECC). Moreover, larvae fed EECC, show the lowest TBARS content, which indicates less lipid

peroxidation. The results showed that granulomas first appeared in meagre larvae at 20 dph when fed rotifers only.

Conversely, a reduced appearance of granulomas and lipid peroxidation occurs when Artemia is included in the feeding

sequence and when the subsequent microdiet contains high levels of vitamin E (800 mg.kg-1) and C (2.000 mg.kg-1), at

44dph.

P.6.44 - 1


Presentation:




AUTHOR 1: Presenting Author Prof. MARGARIDA MARIA BARROS

UNESP - FMVZ - AquaNutri

AUTHOR 2: Mr. PEDRO H. V. ALMEIDA

(1)

AUTHOR 3: Prof. LUIZ EDIVALDO PEZZATO

F(1)

AUTHOR 4: Ms. HINGLIDJ C. MULLER

(1)

AUTHOR 5: Mr. WILLIAM S. XAVIER

(1)

AUTHOR 6: Dr. PEDRO L. P. F. CARVALHO

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.44 - 2

Title:

“Dietary vitamin C supplementation stimulates fin regeneration process in zebrafish (Danio rerio)”

Summary:

Zebrafish (Danio rerio) has become a widely used model organism in studies of neurobiology, developmental biology,

toxicology, and molecular genetics. This small teleost has also been proposed as a possible experimental model for nutrition

and growth studies in fish. However, little is known about the nutritional requirements of this important fish species,

especially considering micronutrients. Among the micronutrients, ascorbic acid (AA) plays key roles on fish growth, bone

development, health, antioxidant status and tissue repairing. Therefore, this study evaluated the influence of dietary vitamin

C as ascorbyl-phosphate on caudal fin regeneration of experimentally amputated zebrafish. Six groups of fish (28 days post-

hatching) were fed experimental diets containing 0, 75, 150, 300, 600 and 1200 mg AA kg-1 diet, for eight weeks. Six fish per

treatment were then experimentally wounded, and sampled at 5, 10 and 15 days post-amputation (DPA) for the

measurement of regenerated area (Leica Qwin v.3.0 system). Experimental diets were continued during the sampling period.

No significant differences were observed at 10 and 15 DPA, regardless the AA supplementation level. However, fish fed AA-

rich diets up to 600 mg AA kg-1 diet presented significantly higher tissue regeneration at 5 DPA if compared to the control

group, but further supplementation did not elicit additional improvements according to the Tukey’s test (P > 0.05).

Regression analysis of tissue regeneration data using the broken-line model resulted in a minimum vitamin C requirement of

897.4 mg kg-1 diet. Our results show that dietary vitamin C influences the rate of caudal fin regeneration in zebrafish. Thus,

dietary vitamin C supplementation could be used as a nutritional strategy preceding handling and/or to accelerate the

healing process of injured animals held under laboratory conditions.

P.6.45 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Christian Lückstädt

ADDCON GmbH

AUTHOR 2:

AUTHOR 3:

AUTHOR 4:

AUTHOR 5:

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.45 - 2

Title:

“Impact of dietary potassium diformate on bacterially-challenged tilapia under commercial conditions”

Summary:

Global production of farmed tilapia in more than 140 countries exceeded 5.7 million t in 2017, an increase of almost 130

percent in ten years. This tremendous growth in production requires high-quality fish feeds. Dietary organic acids, and

especially potassium diformate (KDF) – the most widely tested organic acid salt in aquaculture, are among the various

alternatives spearheading the drive towards environmentally friendly and nutritionally-sustainable aquaculture, which

improves performance and survival of fish. Dietary KDF has been used in tilapia aquaculture since 2005. Trials on its

antimicrobial impact (e.g. against Vibrio spp. or Streptococcus agalactiae) were previously mainly carried out under research

conditions only. This study is among the first to analyse the impact of the additive on juvenile tilapia in commercial cage

culture under challenged grow-out conditions in Northern Malaysia.

A trial was carried out to observe the impact of KDF on fish mortality, caused by higher bacterial levels in the water, among

them Streptococcus agalactiae, using Nile tilapia, Oreochromis niloticus, of approx. 450 g live weight. Water temperature of

the lake was 30°C throughout the trial. Two cage systems, located next to each other (each HDPE cage with a volume of

~1885 m3) were used for the study. One system served as the negative control, whereas fish in the other system were fed

the commercial diet (31% CP) which included 0.4% KDF. The duration of the trial was 60 days; monitoring of mortality

(number of fish and biomass) started on day 27 after feeding the additive. Data were subjected to statistical analysis and a

significance level of 0.05 was used in all tests.

Tilapia fed with 0.4% KDF had significantly lower mortality numbers compared to the control (P=0.023) – as measured over a

period of 33 days. Furthermore, the overall biomass lost due to mortality was also significantly reduced (P=0.005) due to the

inclusion of KDF. The weight of the dead fish did not differ between control and treatment (P=0.78).

The results show that under commercial conditions, survival rates in tilapia fed with dietary potassium diformate are

improved under conditions of bacterial challenge, thus confirming data from laboratory trials.

P.6.46 - 1


Presentation:




AUTHOR 1: Ms. Denisse González-Colunga

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León

AUTHOR 2: Ms. Maribel Maldonado-Muñiz

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León(1)

AUTHOR 3: Dr. Janet Gutiérrez-Uribe

Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA

AUTHOR 4: Mr. Joseph Selvin

Department of Microbiology, School of Life Sciences, Pondicherry University

AUTHOR 5: Dr. Denis Ricque-Marie

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León(1)

AUTHOR 6: Presenting Author Dr. Lucía Elizabeth Cruz-Suárez

Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.46 - 2

Title:

“An Ecklonia arborea phlorotannins-rich extract as a potential feed additive against Vibrio

parahaemolyticus causative of acute hepatopancreatic necrosis disease (AHPND) in shrimp”

Summary:

AHPND caused by Vibrio parahaemolyticus (Vp) is one of the most important diseases that the shrimp farming industry

worldwide is facing. The objective of this study was to evaluate a phlorotannins-rich extract from the marine brown alga E.

arborea as a potential antimicrobial shrimp feed additive to manage AHPND. Dehydrated E. arborea collected in Baja

California Sur, México, was used to obtain an ethyl acetate fraction (EAF) where the most abundant phlorotannins were

identified by HPLC-DAD and HPLC-MS-TOF as eckol and phloroglucinol derivatives, which have been previously reported as

antibacterial compounds. The antimicrobial activity of the EAF against Vp strain M0904 AHPND+ was evaluated in terms of

minimum inhibitory concentration (MIC). The EAF acute toxicity was determined in two assays on the brine shrimp Artemia

salina by testing 7 concentrations (900 to 360 µg/ml seawater) with 4 replicates where 24 h LC50 was calculated.

Additionally the EAF effect on survival of the white leg shrimp Litopenaeus vannamei (1g body weight) was observed after 6

days of feeding graded doses (0, 1, 10, 100, 1000 mg extract/ Kg shrimp biomass/day) under controlled conditions with 3

replicates and 10 shrimp per tank. The EAF presented high anti Vp activity in vitro with a MIC of 500 µg/ml and presented low

toxicity in Artemia (LC50 674.1 µg/ml) as well as in shrimp (100, 93, 90, 90, 86.7% survival with the respective daily doses

mentioned above). These results show the potential of phlorotannins-rich extracts from E. arborea as antimicrobial additive

in shrimp feeds to manage AHPND.

P.6.47 - 1


Presentation:




AUTHOR 1: Presenting Author Prof. Xiaoming ZHU

Institute of Hydrobiology, Chinese Academy of Sciences

AUTHOR 2: Prof. Dong HAN

(1)

AUTHOR 3: Dr. Haokun LIU

(1)

AUTHOR 4: Prof. Shouqi XIE

(1)

AUTHOR 5: Dr. Junyan JIN

(1)

AUTHOR 6: Mr. Shengping CAO

(1)

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.47 - 2

Title:

“Effects of dietary Spirulina platensis on growth, immune response, disease resistance and gene

expression of Toll-like receptor 2 pathway in gibel carp (Carassius auratus gibelio var. CAS III)”

Summary:

The present study evaluated the effect of diets containing Spirulina platensis powder on growth performance, immune

response and disease resistance of juvenile gibel carp, as well as the gene expression of Toll-like receptor 2 pathway. Four

isonitrogenous (360 g kg-1) and isolipidic (90 g kg-1) diets were formulated to contain 0 g (SP0, the control diet), 3.38 g

(SP3.38), 6.76 g (SP6.76) and 13.52 g (SP13.52) S. platensis powder per 100 g diet to replace 0%, 25%, 50% and 100% of

fishmeal protein, respectively. Each diet was randomly assigned to triplicate tanks with 22 fish (about 15 g) per tank. After

the growth experiment, a challenge test was carried out using Aeromonas hydrophila. The results showed that feeding rate

and specific growth rate of fish in Group SP3.38 and SP6.76 were significantly higher than that in group SP0 and SP13.52.

While feed efficiency and protein retention rate had no significant difference among all the groups. The 7-day cumulative

survival rate after challenge test was highest in the SP3.38 group. Prior to challenge test, superoxide dismutase and

phagocyte activity of blood leukocytes had no remarkable difference among the four groups, while there was a significant

increase in the S. platensis addition groups 12h post bacterial challenge. Both pre and post challenge test, plasma lysozyme

activities in the S. platensis supplemented groups were significantly higher than that in the control group. Plasma

malondialdehyde got the lowest value in the SP13.52 group before and after challenge test. The transcriptional levels of

TLR2, myeloid differentiation factor 88 (MyD88), Toll/IL-1 receptor domain-containing adaptor protein (TIRAP), interleukin-1β

(IL-1β) and tumor necrosis factor-α1 (TNF-α1) in spleen and kidney significantly increased post bacterial challenge compared

to pre challenge. And the relative expressions of the immune related genes of the S. platensis groups were higher than that in

control group before and after challenge test. The present results indicated that low dietary inclusion of S. platensis

significantly enhanced the immune response of gibel carp partly through TLR2 pathway and 3.38% of dietary S. platensis was

recommended for the juveniles based on the growth and immune response.

P.6.48 - 1


Presentation:




AUTHOR 1: Presenting Author Dr. Alicia Estévez

IRTA, Centre de Sant Carles de la Ràpita, Unitat de Cultius Experimentals

AUTHOR 2: Ms. Joana Firmino

(1)

AUTHOR 3: Dr. Clara Trullàs

TECNOLOGIA & VITAMINAS S.L.

AUTHOR 4: Dr. Elisabet Vilarrassa

TECNOLOGIA & VITAMINAS S.L.

AUTHOR 5: Dr. Enric Gisbert

(1)

AUTHOR 6:

AUTHOR 7:

AUTHOR 8:

AUTHOR 9:

AUTHOR 10:

AUTHOR 11:

AUTHOR 12:

P.6.48 - 2

Title:

“Use of essential oils in feeds for gilthead seabream for preventing parasitic infections by Sparicotyle

chrisophrii”

Summary:

Two microencapsulated essential oils (garlic oil –Arotec-A– and a mixture of garlic oil with carvacrol and thymol –Arotec-G–,

TECNOLOGÍA & VITAMINAS, S.L., Spain) were used in the formulation of diets for gilthead seabream (Sparus aurata). The

diets were used to feed 40 g gilthead seabream for 30 days. The experiment was carried out in triplicate tanks in IRTA using

recirculation units. After 30 days of feeding, part of the fish were used in a cohabitation trial with fish infected with S.

chrisophrii in a ratio 2 non-parasitized fish (receptors): 1 infected fish (donors), with a total of 36 fish per tank. The

cohabitation trial was carried out for 30 days. At the end, the fish were sampled from each tank separating receptors and

donors. Then, fish were weighted, measured, individually stored in labeled bags and frozen at -20 ºC for parasite

identification and counting. The number of parasites in each of the gills was determined in each individual fish, with 3

categories: adults, juveniles and larvae, according to morphological characteristics. No differences were detected in the

growth of the fish at the end of the study (151 – 158 g). Fish from the control group (diet devoid of essential oils) showed a

significantly higher number of parasites in their gills, whereas fish fed the Arotec-G enriched diet had a significantly lower

number (Figure 1). The presence of adults was significantly higher in the control and Arotec-A fed groups, showing a higher

degree of reinfection with the parasite compared with the Arotec-G group, which showed fewer parasites, mostly juveniles

(Figure 2). Thus, the preventive use of a diet enriched with Arotec-G (microencapsulated garlic oil with carvacrol and thymol)

results in a lower presence of S. chrysophrii when the fish are exposed to highly infected fish.

Fig. 1. (a) Number of parasites per fish fed the experimental diets. Different letters show significant differences. (b)

Percentage of adults, juveniles and larvae of S. chrisophrii in the gills of the fish fed the experimental diets. (ANOVA P<0.001)

(a, b for adults; x, y for juveniles; A, B for larvae).

aisfnf0029 kind of session: poster p.6.01 session topic ...s.a.): control 2 (without essential...

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