all origami shown in this report is from the same annealing run that massud showed in his 6/21/2011...
TRANSCRIPT
All origami shown in this report is from the same annealing run
that Massud showed in his 6/21/2011 weekly report
• biotin/streptavidin position on A tile of 2D DNA array (see next slide)staple:m13 5:1
• [m13]=10 nm• SA:biotin staple 15:1 • this annealing run was not dialyzed before adding SA and
imaging
58.88nm
Two biotin on each side of A tile
SA labeled A tile
SA labeled 2D cross DNA origami array
A subunit with total 4 biotin
B subunit with no biotin
+
anneal over weekend
A subunit with total 4 biotin
B subunit with no biotin
+
15:1 SA:biotin staples
AB array X AB array YSA AFM #1 (mica)
AB array X AB array YSA + + 66nM SA (15:1)66nM SA (15:1)
anneal over weekend
+
4 deg C overnight4 deg C overnight
AB array XSA AB array YSASA AFM #2 (mica)
All AFM images shown in final 6 slides of this presentation were previously shown in group meeting by Massud and are of
AB array XSA
below is a flowchart type key to the AFM images in the subsequent slides, any images that are not square can be assumed to have long side (X) length equal to the Y range seen on side of image. Unless otherwise stated, all images on a single .ppt slide are of the very SAME mica surface/depostion. Also, unless stated, deposition was by 5 min. incubation of origami soln. on mica followed by single HOH rinse.
AB array YSA AFM #1 first mica after one HOH rinse
AB array YSA AFM #1 same mica after second HOH rinse
AB array YSASA AFM #2 first mica after one HOH rinse
AB array YSASA AFM #2 second mica one HOH rinse
AB array X AFM #1
AB array XSA AFM #2
this is the first piece of mica imaged, TAE rinsed at left, TAE rinse followed by HOH rinse below
AB array XSA AFM #2 second piece mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; continued next slide
same mica surface/ deposition as seen in previous slide
AB array XSA AFM #2
second peice mica imaged- rinsed x2 with HOH, these images are all of one mica surface/one deposition; the only difference between this slide and the 2 preceding slides is that the same mica surface seen in previous 2 slides, sat in covered dish in ambient conditions for 6 days prior to acquisition of the images on this slide
Massud included the following slides in his 6-21-11 presentation
SA labeled 2D cross DNA origami array (this and all slides following are of
AB array XSA)
SA labeled 2D cross DNA origami array (this and all slides following are of
AB array XSA)
SA labeled 2D cross DNA origami array
SA labeled 2D cross DNA origami array
SA labeled 2D cross DNA origami array
SA labeled 2D cross DNA origami array
SA labeled 2D cross DNA origami array