WHEN NOT HING ELSE MAT T ERS MORE T HAN ACCURAT E RESULTS

In the ever changing world of Biopharmaceuticals, reliable and robust separation technologies are

needed to keep pace with this fast growing area of discovery. System accuracy and reproducibility

mean everything to scientists who stake their reputations on the results they generate or methods

they develop.

The Alliance® HPLC Bioseparations System, a high-precision, high-resolution HPLC system for the

fractionation and analysis of complex biomolecule mixtures, is ideally suited to help accelerate the

progress of your biotechnology analyses.

The core of the Alliance HPLC Bio System is the Waters e2796 Bioseparations Module. Designed

for robust and reliable operation under the harsh salt and pH conditions typical of bioseparations,

it features an inert flow path, making it ideal for protein purification, peptide mapping, and nucleic

acid analysis.

ALLIANCE HPLC BIOSEPARATIONS SyST EM

THE ALLIANCE HPLC BIOSEPARATION SySTEM fEATURES:

n Biocompatible flow path (Titanium/PEEK)

n Auto•Blend™Technology,whichblendsuptofoureluentstoperformisocratic,step,continuousgradient,orflowprogramapplications

n AutomatedSystemPREProutineforrapidunattendedsystemequilibration

n Precisetemperaturecontrolforenhancedsamplestability

n Compatibilitywithhigh-pressureandlow-pressurecolumnchemistries

n Automatedcolumn switching,flowdiversion,andfractionmanagementtosupportcomprehensivemulti-dimensional separationsmethods

n Enhancedsystem monitoringfunctions (pH,conductivity, composition,valve, andfractiontracking) formethodsdevelopment andtroubleshooting

n Provenday-to-dayreproducibility

The Alliance HPLC Bioseparations System may be configured to provide fully automated purification platforms for proteins, peptides, and other biomolecules.

High sensitivity UV/Visible

or PDA detection allows for

the simultaneous quantitation

of both major and minor

components.

Samples can be processed from

standard 96-, shallow- and deep-

well plates, 384-well microtiter

plates and custom plates,

2-mL vials, or 0.5- and 1.5-mL

microcentrifuge tubes.

Available online pH and

conductivity monitors enable you

to optimize complex separations

using real time buffer composition

information.

Sample temperature control

enhances biomolecule stability

prior to analysis.

Titanium flow path is ideal for

biomolecule separations using

all modern bioseparation

chemistries.

Switching

valves expand

the system’s

capability

to include

higher peak

capacity, multi-

dimensional

separations.

BioSuite™ HPLC Columns are

high performance chemistries

developed specifically for the

isolation, analysis, and charac-

terization of proteins, peptides,

and other biomolecules.

Quaternary eluent blending

enables the automatic

formulation of buffers from

simple stock solutions

using Auto•Blend methods.

Solventmanagement

The simplified design of the e2796 Solvent Manager enables the

blending and delivering of solvents in a pulse-free, low-dispersion

flow path.

In addition, Synchronized Composition Control™ (SCC™) of the gra-

dient proportioning valve (GPV) ensures that the e2796 Separations

Module delivers excellent gradient composition accuracy and precision.

The overall design results in reduced complexity and enhanced overall

ruggedness across a flow range that covers two orders of magnitude.

Automatedsystemstart-up

The Alliance HPLC Bio System also features SystemPREP – a

programmable, automated system preparation routine – for fast,

one-touch, walk-up operation. This function performs a series of

automated steps, putting the separations module in a condition of

complete system readiness for running samples. SystemPREP is

useful when you are changing solvents or after extended periods of

system inactivity and is a simple-to-use alternative to running the

individual functions of Wet Prime, Purge Injector, Equilibrate, and

Condition Column.

INT EGRAT ED fLUIDICS fOR CONSIST ENT PERfORMANC E AND ENHANC ED REP RODUCIBIL IT y

The Waters e2796 Bioseparations Module is the core of the system. Its integrated solvent management and sample management provides

consistent performance and enhanced reproducibility for high quality, high-throughput analysis for HPLC, LC/MS, or flow injection-mass

spectrometry applications.

5%

90%

5%

0%

A

B

C

D

Water

Acetonitrile

100 mMAmmonium

Formate

100 mMAmmoniumBicarbonate

Initial Conditions final Conditions

Initial final

90%

5%

5%

0%

5 mMbuffer

5 mMbuffer

Samplemanagement

The design of the e2796 Sample Manager ensures precise sampling

– delivering predictable peak areas, and consistent results.

Complimenting the simplified design of the solvent flow path, a user

programmable needle wash, and selectable “purge-wash-purge”

cycle ensures that any part of the system exposed to sample is

fully washed, virtually eliminating the risk of sample carryover.

The inherent versatility of the three-axis (X, Y, Z) needle assembly

of the e2796 Sample Manager allows for greater applications

flexibility. The sample manager has a wide range of sample loops,

holding loops, and syringe sizes available and can process samples

from a variety of plates, vials, and/or tubes. This variety allows

you to customize the configuration of the sample manager according

to the needs of your applications.

Auto•BlendTechnology

The e2796 Separations Module features Auto•Blend Technology,

which enables you to generate gradients automatically without

having to manually alter the buffer concentration. Auto•Blend

programming technology improves uptime since no premixing of

mobile phases is required.

Independently driven pistons achieve smooth solvent flow – without pulse dampeners – reducing gradient volume

Dual pressure transducers enable true pulse-free solvent flow High-efficiency

vacuum degassing technology

Precise solvent blending delivers highly reproducible gradients

Tool-free routine maintenance on plungers/seals/seal-wash seals

Simplified flow path with only two inlet check valves

BE ASSURED. CHOOSE WATERS GLOBAL SERVICES.

With94officesinmorethan50countries,onlytheWatersGlobalServicesteamhasthemostin-depthandup-to-dateknowledgeofthe

advancedscienceandtechnologiesthatprovidethefoundationforAllianceHPLCSystems.Maximizesystemuptime,increaselaboratory

productivity,andmeetstringentcompliancerequirementswiththeexpertiseandresponsivenessyouneed.

DO MORE WIT H yOUR RESULTS

Waters software solutions enable you to capitalize on the results generated by your system. The Waters Laboratory Informatics

Suite includes proven information management solutions that integrate all your lab resources in a consistent, scalable, and

seamless application environment.

LEADING BIOSEPARAT IONS CONSUMABLES

When your work involves the analysis of proteins, peptides, and amino acids, Waters has a variety

of chemistry consumable solutions for biomolecule separations in areas ranging from proteomics

to the validation of well-characterized biopharmaceuticals.

BioSuiteHPLCColumns

Designed specifically for bioseparations, Waters’ line of BioSuite

HPLC Columns deliver the resolution and recovery of proteins and

peptides scientists need. Available in ion exchange, size exclusion,

hydrophobic interaction, and reversed-phase modes, BioSuite HPLC

Columns are available in analytical as well as “lab scale” prepara-

tive dimensions.

Peptideseparationtechnologycolumns

Peptide Separation Technology is a unique column chemistry that

meets the demanding requirements of peptide separations used in

a range of applications. Available in 130 Å and 300 Å pore sizes,

Peptide Separation Technology columns are available in numerous

particle sizes and column dimensions for consistent separations,

from high-sensitivity proteomics, to peptide mapping, to high mass

load purification. Peptide Separation Technology columns are

specifically QC tested with a protein digest to ensure exceptional

application performance.

Waters BioSuite HPLC Columns.

Empower™ Software provides control

of the system, including data acquisition,

management, processing, and reporting, all

in the market-leading chromatography data

software (CDS) platform. Empower Software

is scalable from a single workstation to an

enterprise-wide CDS solution, and offers

advanced features for method development,

reporting, and custom calculations, as well

as Enterprise Options for advanced data

management and automated method valida-

tion management.

MassLynx™ Software, for advanced

mass spectrometry applications, enables

researchers to acquire, analyze, manage,

and distribute information. MassLynx

Software offers flexible control over complex

instrument configurations, and Application

Managers that are tailored for specific MS

analyses.

NuGenesis® SDMS is a customizable scien-

tific information management platform that

automatically captures and stores data in

a centralized data warehouse. SDMS makes

information easier to access, facilitates

regulatory compliance, and aids in the

administration of intellectual property. SDMS

converts scientific results into accessible and

valuable corporate knowledge.

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WatersCorporation 34 Maple StreetMilford, MA 01757 U.S.A.T: 508 478 2000F: 508 872 1990www.waters.com

Waters, Alliance, and NuGenesis are registered trademarks of Waters Corporation. The Science of What’s Possible, Auto•Blend, BioSuite, Synchronized Composition Control, SCC, Empower, and MassLynx are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2008 Waters Corporation. Produced in the U.S.A.November 2008 720000589EN LB-PDF