Alterations of insulin-like growth factor binding protein 3 (IGFBP-3) glycosylation in patients with breast tumours

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    (IGFBP-3)Breast tumourGlycosylationLectin-afnity chromatography

    gron olycvest

    Design and methods: IGFBP-3 in serum samples from healthy women and from women with breastin terms of its concentration (IRMA), glycosylation moiety (lectin-afnity

    Clinical Biochemistry 43 (2010) 725731

    Contents lists available at ScienceDirect

    Clinical Bioc

    evand tissues. Imbalances within the axis lead to aberrant signallingnetworks and over-expression of growth factors that are implicated inthe development and progression of breast cancer. Insulin-likegrowth factor binding proteins (IGFBP-1 to -6) are multi-functionalregulatory proteins that bind IGFs [2]. They are differentiallyexpressed in cells, tissues and physiological uids. IGFBP-1, -2, -3and -4 are the most abundant IGFBPs in blood. IGFBP-3 is by far themain circulating IGFBP that maintains a circulating reservoir of IGFs,has a role in their transport and prolongs their half-life [3]. Nativenon-glycosylated IGFBP-3 has a molecular weight of 29 kDa. In the

    counterpart. It would appear that post-translational modications ofIGFBP-3 are important and are likely to affect IGFBP-3's regulatoryfunctions in breast tissue. Furthermore, IGFBPs can be cleaved byproteases into forms that possess either reduced or no afnity for IGFs.Prostate-specic antigen (PSA), cathepsin D and plasmin can cleaveIGFBP-3 and all of these proteases have been localised within breastcancer tissue [5].

    Several large prospective and cohort studies have sought toidentify relationships between IGF-I, IGFBP-3 and breast cancer risk. Apositive association between IGF-I and breast cancer risk exists in pre-circulation IGFBP-3 exists as two glycofocontaining 3N-glycosylated sites (Asn-X-SerAsn109 and Asn172 (4, 4.5 and 5 kDa, resglycoform has two occupied N-glycosylat

    Corresponding author. Tel.: +381 11 2 617 252; faxE-mail address: (I. Barievi).

    0009-9120/$ see front matter 2010 The Canadiandoi:10.1016/j.clinbiochem.2010.03.006lopment of the breast byc effects [1]. The axis isolic activity within cells

    glycosylated forms of IGFBP-3 are functionally similar in their abilityto bind IGFs. However, in T47D breast cancer cells glycosylated IGFBP-3 binds less to the cell surface compared to its non-glycosylatedexerting potent mitogenic and anti-apoptotialso a very important regulator of the metabThe growth hormone/insulin-likeis an essential modulator of growth anmore mannose, fucose, bisecting GlcNAc and terminal sialic acid residues.Conclusion: Our results showed that breast cancer progression causes alterations of IGFBP-3

    glycosylation. The extent of changes increases with breast cancer severity. 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.


    growth factor I (GH/IGF-I) axisd deve

    45 kDa glycoform has all three [4]. The presence of N-glycosylationsites affects the ability of the IGFBPs to adhere to the cell surfacewhichcan modify their afnity for IGF. Both non-glycosylated andResults: In patients with benign tumours the concentration and carbohydrate content of IGFBP-3 wasunaltered compared to healthy women. In patients with malignant tumours in most cases these twoparameters were unchanged, but there were women whose concentration of IGFBP-3 was reduced and itsstructure was altered. In non-surviving cancer patients the concentration of IGFBP-3 was signicantlyreduced and these molecules contained a greater amount of biantennary complex type N-glycans havingKeywords:Insulin-like growth factor binding protein-3 chromatography) and distribution of molecular species (immunoblotting).tumours was characterisedAlterations of insulin-like growth factor bpatients with breast tumours

    Ivona Barievi a,, Romana Masnikosa a, Dragana Laa Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Banatska 3b Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

    a b s t r a c ta r t i c l e i n f o

    Article history:Received 13 October 2009Received in revised form 3 March 2010Accepted 10 March 2010Available online 19 March 2010

    Objectives: Insulin-likedevelopment and progressiand IGF-II. Changes in the gaim of this study was to in

    j ourna l homepage: www.e lsrms of 40 and 45 kDa/Thr) located at Asn89,pectively). The 40 kDaion sites whereas the

    : +381 11 2 618 724.

    Society of Clinical Chemists. Publishding protein 3 (IGFBP-3) glycosylation in

    ndin a, Vera Golubovi b, Olgica Nedi a

    11080 Belgrade, Serbia

    owth factor binding protein-3 (IGFBP-3) is an important modulator off breast cancer as it regulates the amount of free, physiologically active IGF-Iosylation pattern within IGFBP-3 may affect its interaction with ligands. Theigate whether such changes occur during disease progression.


    i e locate /c l inb iochemmenopausal patients [68]. No evidence supports a role for IGF-I inpredicting post-menopausal risk [913]. Evidence linking IGFBP-3 tobreast cancer risk is inconsistent. The consequence of high circulatinglevels of IGFBP-3 on breast cancer risk in some studies is associatedwith increased breast cancer risk in pre-menopausal woman whereasother studies report a protective role, concluding that high IGFBP-3concentrations are associated with decreased cancer risk [7,12,14].Recent meta-analyses indicate a positive association between

    ed by Elsevier Inc. All rights reserved.

  • 726 I. Barievi et al. / Clinical Biochemistry 43 (2010) 725731circulating IGFBP-3 levels and pre-menopausal cancer risk [15]. Incontrast, women with high IGF-I and low IGFBP-3 tend to be at highrisk for carcinoma in situ [16]. Careful scrutiny of the circulating formsof IGFBP-3 has been suggested. Rinaldi et al. demonstrated that highlevels of functional IGFBP-3 (intact IGFBP-3 and some IGFBP-3fragments) could be associated with reduced breast cancer risk,whereas high levels of total IGFBP-3 (intact and fragmented) could beassociated with increased breast cancer risk [17]. However, Li et al.failed to nd any association of intact, fragmented or total IGFBP-3with breast cancer risk [18]. Espelund et al. reported that in breastcancer patients total IGFBP-3 was unchanged, whereas intact IGFBP-3was increased due to reduced IGFBP-3 protease activity [19]. Contraryto most other studies, a recently performed large prospective studyrevealed an association between IGF-I, IGFBP-3 and breast cancer riskin post-menopausal woman [10]. The prospective Nurses' HealthStudy II noted that both IGF-I and IGFBP-3 were not associated withbreast cancer risk in pre-menopausal patients [20].

    The results from several studies that have investigated therelationship between IGF-I, IGFBP-3 and breast cancer risk are,therefore, unclear and contradictory. Such inconsistencies warranteda re-examination of the role of the IGF system in breast tumours. Inour current study we have measured the concentrations of IGF-I andIGFBP-3 in patients diagnosed with breast cancer (benign ormalignant) and were indicated for surgery. Our goal was not tocompare the levels of IGF-I and IGFBP-3 between pre-menopausal andpost-menopausal woman, rather we sought to determine whetherchanges in the glycosylation pattern of IGFBP-3 were involved in theoverall alteration of the circulating IGF/IGFBP system and whetherthese changes were related to malignancy. As protein glycosylationinuences stability and function of the IGF system components and aschanges in N-linked glycosylation occur during cancer development,we characterised the carbohydrate moiety of IGFBP-3 in breasttumour patient groups using eight lectin-afnity columns withdifferent carbohydrate specicities. This allowed us to identifysaccharide residues present in IGFBP-3 from patients and to comparethe glycomic prole with that characteristic for IGFBP-3 from healthywomen. Another approach was to investigate the presence andrelative abundance of different IGFBP-3 molecular species in patientswith breast tumour. Therefore, our aim was primarily focused onalterations of IGFBP-3 glycosylation during disease progression. Thesealterations may reect IGFBP-3 stability, half-life, binding capacityand, as a consequence, they may regulate IGF bioavailability.


    Study population

    Serum samples were obtained from healthy adult women (n=20,age 5010.2 years) and from women diagnosed with breast tumourindicated for surgery (n=53, age 5711.3 years). All womencompleted a questionnaire at interview containing details of personalcharacteristics and reproductive history. Patients were treated withchemotherapy for several months prior to surgery in the Institute ofOncology and Radiology of Serbia, Belgrade, Serbia during 2007.Patients included in this study were those indicated for surgery in theperiod JulyAugust 2008. After post-operative histopathologicalanalysis and follow up that lasted 1 year after the surgery the patientswere divided into three groups: group 1 patients diagnosed withbenign breast disease, survivors (n=8, age 5811.6 years), group2 patients diagnosed with breast cancer, survivors (n=36, age 5611.6) and group 3 non-surviving patients diagnosed with breastcancer (n=9, age 5510.3). All the patients were also consideredclustered as pre-menopausal and post-menopausal women. Thesesubgroups, however, did not show any differences in the measuredparameters. Therefore, we decided not to analyse them further as

    such. 13% of patients underwent short surgical interventions(tumorectomia, up to 30 min) whereas 3% of the patients hadextensive surgery (resectio partialis mamme). 52% of patientsunderwent longer operations (Mastectomia radicalis that lastedfrom 50 to 175 min) whereas 32% of patients underwent Quad-rantectomia cum dissectioaxillae dex (surgery lasted from 70 to140 min).

    The BMI of the volunt