altering taproot architecture of black tupelo (nyssa sylvatica) to a more fibrous root system

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ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM Jimmy Klick, M.S. - Urban Forestry Advisor: Dr. David J. Williams

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I presented this powerpoint at a national conference for the American Society of Horticultural Sciences in St. Louis on July 27, 2009. ABSTRACTThe demand for native plants in the urban landscape continues to increase. Traditional plant production methods in nurseries have had limited success in producing saleable plants from some of our native trees. One of the major difficulties relates to species that produce a taproot. I investigated alternate container production methods in a tree native to Illinois with multiple seasons of interest, the black tupelo (Nyssa sylvatica). Black tupelo is rarely found in the urban landscape because of a coarse root system rendering it difficult to transplant. Three existing root promotion techniques, air-root pruning, manual root pruning or auxin (K-IBA) dips, were used on container grown young seedlings and two-year-old transplanted liners. My objective was to alter taproot architecture of black tupelo and promote a more fibrous root system to alleviate transplant shock and facilitate field establishment. Results showed that superior root systems were produced using several air-root pruning containers that are also more economically feasible than similar standard containers. Higher concentrations of auxin resulted in an increase in all plant parts measured, with an increase in survival. Manual root pruning produced only minor benefit.

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Page 1: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO

A MORE FIBROUS ROOT SYSTEM

Jimmy Klick, M.S. - Urban Forestry

Advisor: Dr. David J. Williams

Page 2: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Research Introduction

Objective

Page 3: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Research Introduction

Air-root pruning Small Containers

Page 4: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Research Introduction

Air-root pruning Small Containers Medium Containers

Page 5: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Research Introduction

Air-root pruning Small Containers Medium Containers

Auxin (K-IBA) 0 mg/L 1875 mg/L 3750 mg/L 7500 mg/L

Page 6: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Data Collected

Page 7: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

1

3

Root Rating

Density Mass Coarse roots Circling roots Dead roots

Page 8: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Ordinal Logistic Model

Root Rating Why?

Ordinal values No transformation Determines likelihood of something occurring

How does it work? Odds Ratio

Determines how different one treatment is from another

Confidence intervals

Page 9: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Results: Root Rating Small Containerscd ab de de bc a bc ef f

Page 10: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Root Structure (Visual observations)

Fabric Container Plant Band Container

Page 11: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Results: Root Rating Medium Containers

b b

aa

Page 12: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Results: Auxin Root Rating a

Page 13: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Survival Rate

0 mg/L: 40% 1875 mg/L: 75% * 3750 mg/L: 65% * 7500 mg/L: 70% *

* significant difference (P = 0.025)

Page 14: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Conclusions

Small air-root pruning containers Black fabric and Plant Band

Medium air-root pruning containers Black fabric and green mesh

Auxin dips Transplant survivability

Page 15: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Future Studies

Media Transplant Auxin Ordinal Logistics Model

Page 16: ALTERING TAPROOT ARCHITECTURE OF BLACK TUPELO (NYSSA SYLVATICA) TO A MORE FIBROUS ROOT SYSTEM

Acknowledgements

Advisor: Dr. David J. Williams Committee: Dr. Gary J. Kling and Dr. Jeffrey O.

Dawson Joseph Castro and Massimo Bionaz for help with

statistical analysis IR-4 Program for funding my research assistantship High Caliper Growing Systems, Lacebark Inc.,

Nursery Supplies Inc. for providing containers