amersham western blotting - fisher scientific · amersham™ western blotting selection guide. ......
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GE HealthcareLife Sciences
Amersham™ Western blottingSelection guide
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Western blotting selection guide
Product Quantity Fisher Scientific Cat. No.
Description
Sample preparationSDS-PAGE Clean-Up kit • • • 50 samples 45-001-738 Removal of interfering contaminants and concentration of total proteinMammalian protein Extraction buffer • • • 1 × 500 ml 45-002-169 Buffer for extraction of proteins from mammalian cells2-D Quant kit • • • 500 assays 45-001-732 Quantitation of protein amount using a colorimetric assay
Gel electrophoresisAmersham ECL™ Gel Box • • • 1 45-002-481 Horizontal electrophoresis system with virtually no assembly requiredAmersham ECL Gel 10%, 2 wells • • • Pack of 10 45-002-471 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 10 wells • • • Pack of 2 45-002-476 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 10 wells • • • Pack of 10 45-002-461 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 10%, 15 wells • • • Pack of 10 45-002-466 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 2 wells • • • Pack of 10 45-002-472 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 10 wells • • • Pack of 2 45-002-477 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 10 wells • • • Pack of 10 45-002-462 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 12%, 15 wells • • • Pack of 10 45-002-467 Precast horizontal PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 2 wells • • • Pack of 10 45-002-473 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 10 wells • • • Pack of 2 45-002-478 Precast horizontal gradient PAGE gel for protein electrophoresis
Amersham ECL Gel 4-12%, 10 wells • • • Pack of 10 45-002-463 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-12%, 15 wells • • • Pack of 10 45-002-468 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 2 wells • • • Pack of 10 45-002-474 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 10 wells • • • Pack of 2 45-002-479 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 10 wells • • • Pack of 10 45-002-464 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 8-16%, 15 wells • • • Pack of 10 45-002-469 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 2 wells • • • Pack of 10 45-002-475 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 10 wells • • • Pack of 2 45-002-480 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 10 wells • • • Pack of 10 45-002-465 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel 4-20%, 15 wells • • • Pack of 10 45-002-470 Precast horizontal gradient PAGE gel for protein electrophoresisAmersham ECL Gel running buffer • • • 10 runs 45-002-482 Pre-made 10 × concentrated Laemmli buffer for denatured protein electrophoresisEPS 301 Power Supply • • • 1 45-000-465 Power supply suitable for the Amersham ECL Gel systemDeep Purple Total Protein Stain • 25 ml 45-001-299 Rapid, ultra-high sensitivity fluorescent total protein stain. Suitable for both
protein gels and blots.Bromophenol Blue • • • 10 g 45-000-242 Dye for visualization of the migration of proteins in gel electrophoresis
MarkerLow-Range Rainbow™ Molecular Weight Markers • • 250 μl 45-001-593 Mr 3 500 to 38 000, seven separate proteins with five different visible colors, not
visible on chemiluminescence imageHigh-Range Rainbow Molecular Weight Markers • • 250 μl 45-001-592 Mr 12 000 to 225 000, eight separate proteins with six different visible colors, not
visible on chemiluminescence imageFull-Range Rainbow Molecular Weight Markers • • 250 μl 45-001-591 Mr 12 000 to 225 000, ten separate proteins with six different visible colors, not
visible on chemiluminescence imageAmersham ECL DualVue Western blotting markers • • 25 loadings 45-001-182 Detect proteins on gel and membrane as well as on film, Mr 15 000 to 150 000
with 3 bands visible on gel and blotting membrane and 7 bands visible on chemiluminescence image
Amersham ECL Plex Fluorescent Rainbow markers • 120 μl 45-001-594 Protein visible molecular weight markers, as well as fluorescent images using Cy3 and Cy5 channels. Mr 12 000 to 225 000
Amersham ECL Plex Fluorescent Rainbow markers • 500 μl 45-001-595 Protein visible molecular weight markers, as well as fluorescent images using Cy3 and Cy5 channels. Mr 12 000 to 225 000
Blotting paper3MM Chr 20 × 20 cm • • • 100 sheets 05-716-3V Blotting paper suitable for Western blot
Blotting sandwichProtran BA83, 0.2 μm blotting sandwich (8 × 7.5 cm) • • 10 units 09-925-109 Pre-cut Protran membrane and blotting paper to fit with the ECL Gel system for
Western blottingProtran BA85, 0.45 μm blotting sandwich (8 × 7.5 cm) • • 10 units 09-925-110 Pre-cut Protran membrane and blotting paper to fit with the ECL Gel system for
Western blotting
MembranesAmersham Hybond™ ECL (20 × 20 cm) • • • 10 sheets 45-000-839 Unsupported, 100% pure nitrocellulose membrane validated for use with all ECL
Western blotting systems and for all protein blotting applications.Amersham Hybond ECL (30 cm x 3 m) • • • 1 roll 45-001-929 Unsupported, 100% pure nitrocellulose membrane validated for use with all
ECL Western blotting systems and for all protein blotting applications. Amersham Hybond-LFP (20 × 20 cm) • 10 sheets 45-001-313 Low-fluorescent PVDF membrane optimized for fluorescent Western blotting applicationsAmersham Hybond-LFP (30 cm x 3 m) • 1 roll 45-001-315 Low-fluorescent PVDF membrane optimized for fluorescent Western blotting applications
Pre-cut to fit with the ECL Gel systemAmersham Hybond-P (20 × 20 cm) • • 10 sheets 45-000-841 Hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in
protein transfers and/or Western blotting applicationsAmersham Hybond-P (30 cm x 3 m) • • 1 roll 45-000-931 Hydrophobic polyvinylidene difluoride (PVDF) membrane optimized for use in
protein transfers and/or Western blotting applications. Pre-cut to fit with the ECL Gel system
Chemiluminesc
ence/F
ilm
Chemiluminesc
ence/C
CD
Fluoresc
ence
Many types of products are available in other formats and pack sizes. Please visit www.fishersci.com for more information.
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Transfer units Quantity Fisher Scientific Cat. No.
Description
TE 22 Mini Tank Transfer Unit • • • 1 45-001-953 Performs protein and nucleic acid transfers in less than one hour. Transfers four mini-gels up to 9 × 10 cm in about 1 l of buffer
TE 62 Transfer Unit • • • 1 45-001-962 Tank transfer for gels up to 15 × 21 cmTE 70 Semi-Dry Transfer Unit, 14 × 16 cm • • • 1 45-001-963 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hourTE 70 PWR Semi-Dry Transfer Unit, 14 × 16 cm • • • 1 45-001-782 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hour.
Built in power supply TE 77 Semi-Dry Transfer Unit, 21 × 26 cm • • • 1 45-001-968 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hourTE 77 PWR Semi-Dry Transfer Unit, 21 × 26 cm • • • 1 45-001-783 Semi-dry electrotransfer proteins from polyacrylamide gels in less than one hour.
Built in power supply EPS 2A200 Power supply • • • 1 450-01-974 Power supply suitable for Western blotting
Blocking agentAmersham ECL Blocking agent • • • 40 g 45-001-197 For blocking nitrocellulose and PVDF membranes to be used with e.g.
ECL Western blotting detection reagentsAmersham ECL Prime Blocking reagent • • • 40 g 45-001-216 For blocking nitrocellulose and PVDF membranes to be used with e.g.
ECL Prime and ECL Plex Western blotting detection reagents
Secondary antibodyAmersham ECL Mouse IgG, HRP-linked whole Ab (from sheep)
• • 1 ml 45-000-679 Secondary antibody for using with e.g. ECL or ECL Prime
Amersham ECL Human IgG, HRP-linked whole Ab (from sheep)
• • 1 ml 45-000-681 Secondary antibody for using with e.g. ECL or ECL Prime
Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
• • 1 ml 45-000-682 Secondary antibody for using with e.g. ECL or ECL Prime
Amersham ECL Mouse IgG, HRP-linked F(ab)2 fragment (from sheep)
• • 1 ml 45-000-680 Secondary antibody for using with e.g. ECL or ECL Prime
Amersham ECL Rabbit IgG, HRP-linked F(ab)2 fragment (from donkey)
• • 1 ml 45-000-683 Secondary antibody for using with e.g. ECL or ECL Prime
Amersham ECL Plex™ goat-a-mouse IgG-Cy2 • 150 ug 45-0014-75 Cy-2 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy2 • 150 ug 45-0014-77 Cy-2 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy3 • 150 ug 45-0014-73 Cy-3 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-mouse IgG-Cy3 • 150 ug 45-001-208 Cy-3 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-rabbit IgG-Cy5 • 150 ug 45-001-212 Cy-5 conjugated secondary antibody for fluorescent detectionAmersham ECL Plex goat-a-mouse IgG-Cy5 • 150 ug 45-001-211 Cy-5 conjugated secondary antibody for fluorescent detection
Detection reagentAmersham ECL Prime Western blotting reagent • • for 1000 cm2 45-002-401 Chemiluminescent detection of Western blotting using film or CCD camera,
low pg detection levelAmersham ECL Western blotting detection reagent • • for 1000 cm2 45-000-878 Chemiluminescent detection of Western blotting using film or CCD camera,
mid pg detection levelAmersham ECL Western blotting detection reagent • • for 4000 cm2 45-000-875 Chemiluminescent detection of Western blotting using film or CCD camera,
mid pg detection levelAmersham ECL Western blotting detection reagent • • for 6000 cm2 45-001-160 Chemiluminescent detection of Western blotting using film or CCD camera,
mid pg detection levelECF Western blotting reagent pack mouse • For 2500 cm2 45-000-943 Alkaline phosphatase conjugated secondary antibodies are used for the detection
of chemifluorescent proteinsECF Western blotting reagent pack rabbit • For 2500 cm2 45-000-945 Alkaline phosphatase conjugated secondary antibodies are used for the detection
of chemifluorescent proteins immobilized on membranesAmersham ECL Select™ • • For 1000 cm2 45-000-999 Chemiluminescent detection of Western blotting using film or CCD camera,
low pg detection level
Detection with filmAmersham Hyperfilm™ ECL (5 × 7 inches) • 50 sheets 45-001-504 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (18 × 24 cm) • 50 sheets 45-001-505 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (8 × 10 inches) • 50 sheets 45-001-507 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm ECL (24 × 30 cm) • 50 sheets 45-001-509 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (18 × 24 cm) • 100 sheets 45-002-808 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (5 × 7 inches) • 100 sheets 45-002-806 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL PrimeAmersham Hyperfilm Blue (8 × 10 inches) • 100 sheets 45-002-807 Suitable for use with all blue and green light chemiluminescent system, such as
Amersham ECL and Amersham ECL Prime
Chemiluminesc
ence/F
ilm
Chemiluminesc
ence/C
CD
Fluoresc
ence
Many types of products are available in other formats and pack sizes. Please visit www.fishersci.com for more information.
![Page 4: Amersham Western blotting - Fisher Scientific · Amersham™ Western blotting Selection guide. ... ECL Prime and ECL Plex Western blotting detection reagents Secondary antibody Amersham](https://reader034.vdocuments.net/reader034/viewer/2022050814/5acc5edd7f8b9ab10a8c6c7f/html5/thumbnails/4.jpg)
BN0524125 05/2012
ECF Amersham ECL Amersham ECL Prime Amersham ECL Select Amersham ECL Plex
Detection technique Chemifluorescence Chemiluminescence Chemiluminescence Chemiluminescence Fluorescence
Limit of detection Mid picograms 20-100 pg Mid picograms 10-100 pg Low picograms < 10 pg Low picograms < 5 pg Low picograms 1-50 pg
Marker Rainbow marker DualVue markersRainbow markers
DualVue markersRainbow markers
DualVue Rainbow marker ECL Plex Fluorescence Rainbow marker
Blotting membrane Hybond-P Hybond ECL
Hybond ECLHybond-P
Hybond-PHybond ECL
Hybond-P Hybond ECL
Hybond-LFPHybond ECL
Primary antibody dilution 1:100 – 1:5000 1:100 – 1:5000 1:1 000 – 1:50 000 1:5000 – 1:30 000 1:100 – 1:5000
Secondary antibody dilution 1:2500 – 1:10 000 1:1 000 – 1:15 000 1:50 000 – 1:250 000 1:100000 – 1:300 000 1:1 250 – 1:4 000
Dynamic range of detection High to medium Medium to very low high to medium High to low High to very low
Fisher Scientific Cat. No. — 45-000-875 45-002-401 45-000-999 —
GE, imagination at work, and GE monogram are trademarks of General Electric Company.
© 2012 General Electric Company—All rights reserved. First published Jan. 2012.