amino acids omar a. alomair biochemistry 1 references biochemistry (lippincott's illustrated...
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Amino Acids
OMAR A. ALOMAIRBiochemistry 1
ReferencesBiochemistry (Lippincott's Illustrated Reviews Series), 6E
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Amino Acid Structure
•More than 300 amino acids have been discover• In mammals, 20 amino acids make up every protein• Each amino acid contains a carboxyl group• Each amino acid contains a primary amino group except for
proline which has a secondary amino group• A side chain (R) is present in every amino acid and is connected to
the α carbon
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Amino Acid Structure
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Amino Acid Structure
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Amino Acid Structure
• The human body function at a physiological pH of 7.4• At this physiological pH, both the carboxyl group and the amino
group dissociate• The carboxyl group dissociate to give the a negatively charged
carboxylate ion (– COO–)•While the amino part is protonated to form a positively charged
amine (– NH3+)• The role of any amino acid is decided according to the nature of
the side chain (R)
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Amino Acid Structure
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Amino Acid Classification
• Each amino acid is classified according to the chemical properties of the side chain• The classification is according to the distribution of the electron in
the R group• As a result, the 20 amino acids are divided into non-polar polar
and amino acids• Polar amino acid is further classified at physiological pH to 1- No charge polar amino acids 2- Positively charged amino acids 3- Negatively charged amino acids
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Non-Polar Amino Acids
• Non-Polar amino acids has the same number of amino and carboxyl groups• They has hydrophobic characteristic • They have no charge on the side chain• They have low water solubility • The create the core of most globular proteins
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Non-Polar Amino Acids
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Non-Polar Amino Acids
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Polar Amino AcidsUncharged
• Polar amino acids that dose not have the same number of amino and carboxyl groups• They have no charge on the side chain• They are mostly involved in forming hydrogen bonds in globular
protein • The sulfhydryl(thiol) group (–SH) of cysteine is important in
creating disulfide bond in extra cellular protein, e.g. Albumin• Phosphate group, which is involved in important physiological
activity binds to the hydroxyl group of serine and thyronine
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Polar Amino AcidsUncharged
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Polar Amino AcidsPositively Charged
• Polar amino acids that does not have the same number of amino and carboxyl groups• They have a positive charge on the side chain• They are basic in nature• Histidine play an important role as a buffer moiety in many
protein• This due to its unique ability to be deprotonated or protonated
when its incorporated into a protein depending on the protein environment
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Polar Amino AcidsPositively Charged
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Polar Amino AcidsNegatively Charged
• Polar amino acids that does not have the same number of amino and carboxyl groups• They have a negative charge on the side chain• They are acidic in nature• They are also called dicarboxylic mono-amino acids• Glutamic acid and aspartic acid are essential as a fuel
intermediate in glycolysis and gluconeogenesis
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Polar Amino AcidsNegatively Charged
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Amino AcidsSources
• The main source of amino acids is diet •Meat provide all the 20 amino acids involved in protein synthesis• Since meat contain all the 20 amino acids it is called “complete
protein”• Some plant act as a source for most of the aforementioned amino
acid but not all of them • Examples of “incomplete protein” include legumes, seeds and
vegetable
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Amino AcidsSources
• The main source of amino acids is diet •Meat and egg white provide all the 20 amino acids involved in
protein synthesis• Since meat and egg white contain all the 20 amino acids it is called
“complete protein”• Some plant act as a source for most of the aforementioned amino
acid but not all of them • Examples of “incomplete protein” include legumes, seeds and
vegetable
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Amino AcidsEssential and Non-Essential
• Another classification amino acids is according to the ability of the body to produce them • Essential amino acids are the ones the normal adult body can not
synthesis and need an external sources to supplement • Non-Essential amino acids include all the amino acids that the
healthy adult human synthesis de-novo • Conditional-Non-Essential amino acids are the amino acids that only
children, elderly and people with diseases such as phenylketonuria need to have in their diet
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Amino AcidsEssential and Non-Essential
Source:https://amit1b.files.wordpress.com
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Amino AcidsDigestion and Absorption
• Hydrochloric acid, released from parietal cells, activate the zymogen pepsinogen to form pepsin enzyme • Pepsin enzyme unfold and break down protein to form short
peptides• It is released form the chief cells lining the stomach• Another proteolytic enzyme is trypsin which is produced in
pancreases and released in the duodenum• The result of the breakdown is single amino acids and short
peptides which can readily be absorbed
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Amino AcidsDigestion and Absorption
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Amino Acids Non-Protein functionMolecules Precursors
• Numerous Amino acids act as starting block of not only protein but also other molecules• These molecules involve neurotransmitter, porphyrins and
nucleotide• Example of this is the neurotransmitter serotonin which is
synthesized from tryptophan • Another Example is the function of glycine as heme porphyrin
precursor
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Amino Acids Non-Protein functionMolecules Precursors
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Amino Acids Non-Protein functionMolecules Precursors
• Numerous Amino acids act as starting block of not only protein but also other molecules• These molecules involve neurotransmitter, porphyrins and
nucleotide• Example of this is the neurotransmitter serotonin which is
synthesized from tryptophan • Another Example is the function of glycine as heme porphyrin
precursor
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Amino Acids Non-Protein functionUrea Cycle
• The urea cycle is one the early biochemical process discovered• It takes place mainly in the liver• The main function of the urea cycle is to convert the toxic
ammonia (NH3) to the much less toxic urea molecule (CH4N2O)• Arginine plays an essential role in the cycle as an intermediate to
remove excess nitrogen• Argininemia is a disease associated with high level of arginine and
ammonia
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Amino Acids Non-Protein functionUrea Cycle
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Amino Acids Non-Protein functionNucleotide biosynthesis
• The main molecule that form both DNA and mRNA• Each nucleotide consist of Phosphate
connected to sugar, the sugar it self is bond to nitrogen base• A number of amino acid is salvaged to
provide structural component to the nitrogen base through the process of nucleotide biosynthesis
Source:https://www.wikiwand.com/en/Nucleotide
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Amino Acids Non-Protein functionNucleotide biosynthesis
Source:https://www.wikiwand.com/en/Nucleotide