amnion biosciences

Amnion Biosciences

About us

“Amnion Biosciences ” is incorporated with wholly owned private equity by pioneering

bioentrepreneurs. The company started with a vision to cater the core services and solutions in

the field of Biotechnology. The unique strength of the company is that our researchers are well

equipped with knowledge and expertise to understand the ever changing demand in the

industry and offer suitable products and solutions as per the need of scientific community with

an unstinted professional integrity. We are pleased to introduce ourselves as the manufacturers

of molecular bio products (research kits, enzymes, markers, ladders and reagents) and we are also

well equipped to take contract research programs as per the prevailing and internationally laid

standards with mutually benefiting confidentiality providing a series of services in the filed of

Biotechnology.

Amnion’s Bio products are developed on your precise experimental needs with a focus on

innovation to nourish science. These products are manufactured to meet international standards

by employing world class technology with uncompromising Quality practices.

Mission

§ To support our customers to achieve outstanding success in the field of biological sciences

§Our commitment for our customers and market is to provide exceptionally quality products and value for money

§Empowering scientific activities by the fastest delivery , with out compromising on the quality of products

§Supporting scientists to accomplish their goals for the enrichment of the scientific community in India

Strengths

§Internationally and nationally renowned scientists as the leaders of our projects

§Exceptional talent, skill, passion, commitment and sincerity of our employees

§Well equipped laboratory

§Quality products

§Collaborations with international organizations

Contents Amnion BioSciences | 2011-12

1 Amnion Services 1

2 Ladders and Markers 3

3 PCR Reagents 6

4 PCR Research Kits 10

5 Research Kits 12

6 Biosolutions 17

7 Terms & Conditions 20

S.No. Category Page No.

2

Amnion Services Amnion BioSciences | 2011-12

Bacterial Identification

• Phylogenetic Tree provided, 10 nearest neighbour information provided

• ~1500 bp aligned sequence data provided

8,500

Per Sample

Per Sample

AMCS005Fungal Identification

• 18S rDNA/ 26S rDNA/ ITS based molecular identification

• Phylogenetic Tree provided, 10 nearest neighbour information provided

• data provided respectively

~ 2Kb/ 2.5Kb/ 500 bp aligned sequence

9,000

9,000

AMCS006 Fish Identification

• molecular identification

Mitochondrial 16S rDNA based

• neighbour information provided

Phylogenetic Tree provided, 10 nearest

• ~ 500 bp aligned sequence data provided

AMCS007Bacterial Population Study

• within Samples provided

Upto 100 nos. Bacteria Identification

• Phylogenetic Tree / analyzed data of all bacteria provided

• ~1500 bp sequence data provided

• Clones of ~1500 bp 16S rDNA genes

Per Bacteria 6,000

AMCS008Protein expression and purification services

• ~90% pure proteins on PAGE

• Expression of gene of interest

• antibody, assay development, etc.

Suitable for polyclonal or monoclonal

Per Protein 90,000

AMCS001

AMCS002

AMCS003

Cloning & Sequencing of PCR Products

Cloning & Sequencing: Upto 0.5 kb

Cloning & Sequencing: Upto 1 kb

Cloning & Sequencing: Upto 2 kb

Per Sample

Per Sample

Per Sample

14,000

21,000

32,000

AMCS004

16S rDNA based molecular Identification• Per Sample

AMCS009Synthetic Gene Service

• verification for fragments upto 1000 bp

Synthesis, Cloning and Sequence Per bp 100

Prices may vary depending upon the Project requirement.

Cat. No. Service Service Description Unit Price (INR)

1

AMCS010Microsatellite Library Development

• library for any organism

Development of Microsatellite

• standardization for specific Markers

Primer designing and PCR

• sequence data provided

Protocols ,primer,

For 20 Markers

210,000

For40 Markers

400,000AMCS011

AMCS012DNA isolation & Purification from any source

• Isolated by Spin Column based protocol

• with Agarose Gel photo

High quality DNA provided

• downstream applications

DNA suitable for all

Per Sample

Per Sample

Enquire

EnquireAMCS013

Molecular Marker Analysis

• SSR, RAPD, Candidate gene Analysis

• Primer designing and data provided

Prices may vary depending upon the Project requirement.

Cat. No. Service Service Description Unit

Amnion Services Amnion BioSciences | 2011-12

Price (INR)

2

AMLD001

AMLD002

250 gµ

250 µg

9,500

8,750

Note: For CUSTOM orders minimum order value should be Rs. 1,000/-

100 bp Ladder

500 bp Ladder

• Ready-to-load• 100 bp to 1 Kb, 10 bands

• Spiked 500 bp as reference

• Excellent run in 2% Agarose

AMLD001S 50 µg 2,000100 bp Ladder • Loading Dye separate• 100 bp to 1 Kb, 10 bands

• Spiked 500 bp as reference


AMLD002S 50 µg 2,100500 bp Ladder • Loading Dye separate• 500 bp to 5 Kb, 10 bands

• Spiked 2.5 Kb as reference

• Excellent run in 1.5% Agarose

AMLD003

AMLD003S

250µg

50 µg

8,750

2,100

1 kb Ladder

1 kb Ladder

1 kb Ladder

• Ready-to-load

• Loading Dye separate

• 1 Kb to 10 Kb, 10 bands

• 1 Kb to 10 Kb, 10 bands

• Spiked 5 Kb as reference

• Spiked 5 Kb as reference






AMLD001C

AMLD002C

AMLD003C

100 bp Ladder

500 bp Ladder

• Order as per requirement



CUSTOM

CUSTOM

CUSTOM

50/µg

45/µg

45/µg

Cat. No. Product Product Description Pack Size

Ladders and Markers Amnion BioSciences | 2011-12

Price (INR)

3

AMLD004 250 gµ 11,500Low Range DNA Ruler


• Spiked 500 bp/ 1 kb as reference


AMLD004C Low Range DNA Ruler

• Order as per requirement CUSTOM 55/µg

AMMR001C Lambda DNA / Eco RI Digest

• Order as per requirement CUSTOM

AMLD005C High Range DNA Ruler

• Order as per requirement CUSTOM 40/µg

20/µg

AMLD004S 50 µg 2,200LowRange DNA Ruler

• Loading Dye separate• 100 bp to 5 kb, 16 bands

• Spiked 500 bp/ 1 kb as reference


AMLD005S 25 µg 2,100High Range DNA Ruler

• Loading Dye separate• 500 bp to 10 Kb, 13 bands• Spiked 2.5 Kb as reference• Excellent run in 1% Agarose

AMMR001 250 µg 3,500Lambda DNA / Eco RI Digest

• Ready-to-load

• 6 fragments

• Range: 3530 bp to 21226 bp

100 µg 1,800Lambda DNA / Eco RI Digest

• Loading Dye separate• 6 fragments

• Range: 3530 bp to 21226 bp

AMLD005 250 µg 8,500High Range DNA Ruler

• Ready-to-load

• 500 bp to 10 Kb, 13 bands



AMMR001S

Cat. No. Product Product Description


Price (INR)Pack Size

AMMR002 250 µg 3,500Lambda DNA / Hind III Digest

• Ready-to-load

• 8 fragments

• Range: 125 bp to 23130 bp

4Note: For CUSTOM orders minimum order value should be Rs. 1,000/-


AMMR002C • Order as per requirement CUSTOM

CUSTOM

20/µg

AMMR002S

AMMR003

AMLD004C

AMMR003C

AMMR003C

100 µg

250 µg

CUSTOM

100 µg

1,800

3,500

15/µg

20/µg

1800

Lambda DNA / Hind III Digest

Lambda DNA / Hind III Digest

Lambda DNA /Eco RI / Hind III Double Digest

Lambda DNA




• Ready-to-load



• 8 fragments

• 13 fragments,

• 13 fragments,

AMLD004 250 µg 3,000Lambda DNA • Linear, dsDNA

• 48502 bp size.

AMPL001

AMPL003

AMPL002

AMPL001C

AMPL002C

AMPL003C

100 µg

100 µg

100 µg

CUSTOM

CUSTOM

CUSTOM

6,000

6,000

6,000

70/µg

70/µg

70/µg

pUC18

pBR 322

pUC 19

pUC18

pUC 19

pBR 322

• Circular, dsDNA

• Circular, dsDNA

• Circular, dsDNA




• 2686 bp size.

• 4361 bp size.

• 2686 bp size.








5

Cat. No. Product Description

AMTq001A

AMTq001B

AMTq001C

AMTq002A

1000 units

2000 units

5000 units

1000 units

3,000

6,000

15,000

3,000

Taq DNA Polymerase

Taq DNAPolymerase

Taq DNA Polymerase

Taq DNA Polymerase

• Conc: 1U/µl

• Conc: 1U/µl

• Conc: 1U/µl

• Conc: 1U/µl

• 10X Assay buffer with MgCl2



• 10X Assay buffer, Separate MgCl2

• Controls provided




AMTq002B 2000 units 6,000Taq DNA Polymerase

• Conc: 1U/µl



AMTq002C 5000 units 15,000Taq DNA Polymerase

• Conc: 1U/µl



AMTq003A 1000 units 3,000Taq DNA Polymerase

• Conc: 3U/µl



AMTq001CUS CUSTOM 4/ UnitTaq DNA Polymerase

• Conc: 1U/µl




AMTq002CUS CUSTOM 4/ UnitTaq DNA Polymerase

• Conc: 1U/µl





Product

Amnion BioSciences | 2011-12


6

PCR Reagents

AMTq003B 2000 units 6,000Taq DNA Polymerase

• Conc: 3U/µl




AMTq003C 5000 units 15,000Taq DNAPolymerase

• Conc: 3U/µl



AMTq003CUS CUSTOM 4/ UnitTaq DNAPolymerase

• Conc: 3U/µl




AMTq004CUS CUSTOM 4/ UnitTaq DNAPolymerase

• Conc: 3U/µl




AMTq004A 1000 units 3,000Taq DNAPolymerase

• Conc: 3U/µl



AMTq004B 2000 units 6,000Taq DNAPolymerase

• Conc: 3U/µl



AMTq005A 1000 units 4,000Taq DNA Polymerase

• Conc: 10U/µl



AMTq005B 2000 units 8,000Taq DNAPolymerase

• Conc: 10U/µl



AMTq004C 5000 units 15,000Taq DNAPolymerase

• Conc: 3U/µl



Cat. No. Product DescriptionProduct



7

PCR Reagents

AMTq008CUS CUSTOM 8/ UnitAMTaq Polymerase

-6• Enzyme mix; High Fidelity : 13x10

• For all regular usages, upto 5 kb







9

PCR Reagents

5,000

8,000

4,500

8,000

4,500

8,000

4,000

7,500

AMPC001A

AMPC001B

50 reactions

100 reactions

PCR Core Kit• Controls provided

• 100 bp ladder provided

• Taq polymerase & individual PCR components provided

AMPM001A

AMPM001B

50 reactions

100 reactions

PCR Master-Mix• Aliquoted separately in 50/ 100 PCR vials


• 2X Reaction mix with Taq Polymerase,

AMPM002A

AMPM002B

50 reactions

100 reactions

AMTaq Master-Mix • Aliquoted separately in

50/ 100 PCR vials


• 2X Reaction mix with AMTaq Polymerase, High Fidelity

AMPC002A

AMPC002B

50 reactions

100 reactions

PCR Clean-up Kit

• ~ 80% recovery

• Cleans up excess enzyme, primers and salt

• Ideal for down-stream applications (sequencing / ligation of amplicons)

• Column based quick protocol



PCR Research Kits Amnion BioSciences | 2011-12

Price (INR)Pack SizeProduct

10

AMBIO001 200 µl 3,100dATP Solution 100mM

AMBIO002 200 µl 1,100dATP Solution 10mM

AMBIO003 200 µl 3,100dCTP Solution 100mM

AMBIO004 200 µl 1,100dCTP Solution 10mM

AMBIO005 200 µl 3,100dGTP Solution 100mM

AMBIO006 200 µl 1,100dGTP Solution 10mM

AMBIO007 200 µl 3,100dTTP Solution 100mM

AMBIO008 200 µl 1,100dTTP Solution 10mM

AMBIO009 200 µl 3,100dUTP Solution 100mM

AMBIO010

AMBIO011

4 x 250 µl

4 x 250 µl

2,500

10,500

dNTP Mix

10 mM mix, Final 2.5 mM each

100 mM, Final 25 mM each

AMBIO012

AMBIO013

4 x 200 µl

4 x 200 µl

10,000

2,800

dNTP Set

100mM each

10mM each



PCR Research Kits Amnion BioSciences | 2011-12

Price (INR)Pack SizeProduct

11

AMRK001

AMRK002

AMRK003

AMpurE Plasmid DNA Mini

• Spin Column based quick protocol

• 1-5 ml culture, Upto ~20 µg high quality DNA

• downstream applications

DNA suitable for all

50 Isolations

100 Isolations

200 Isolations

4,500

8,000

15,000

AMRK007

AMpurE Plasmid DNA Maxi

• 100 ml or more culture, Upto ~1 mg high quality DNA

• DNA suitable for all downstream applications

• Swing bucket Rotor Centrifuge required

25 Isolations

AMRK004

AMRK005

AMpurE Plasmid DNA Midi


• 50-100ml culture, Upto high quality DNA

~500 µg



10 Isolations

25 Isolations

5,000

11,000

AMRK006 • Spin Column based quick protocol 10 Isolations 10,000

20,000

AMRK008

AMRK009

AMpurE Plant Genomic DNA Mini


• 100 mg Leaf or soft tissue, Upto ~20 µg high quality DNA


25 Isolations

50 Isolations

4,500

8,000


Research Kits Amnion BioSciences | 2011-12


12

AMRK010

AMRK011

AMpurE Plant Genomic DNA Midi


• 500 mg- 1 gm Leaf or soft tissue, Upto ~500 µg high quality DNA



10 Isolations

25 Isolations

7,000

12,000

AMRK012

AMRK013

AMpurE Plant Genomic DNA Maxi


• 1-2 gm Leaf or soft tissue, Upto ~ 1 mg high quality DNA



10 Isolations

25 Isolations

12,000

22,000

AMRK014

AMRK015

AMpurE Plant Genomic DNA from Seeds


• 50- 100 mg of Seeds, Upto ~20 µg high quality DNA


50 Isolations

100 Isolations

8,000

15,000

AMRK019

AMRK020

AMpurE Fungal Genomic DNA Mini




25 Isolations

50 Isolations

5,000

7,500

AMRK016

AMRK017

AMRK018

AMpurE Bacterial Genomic DNA Mini




25 Isolations

50 Isolations

100 Isolations

2,500

4,500

9,000




13

AMRK021

AMRK022

AMpurE Fresh Blood Genomic DNA Mini


• 200 µl Blood(Unclotted Blood), Upto ~10 µg high quality DNA


50 Isolations

100 Isolations

4,750

9,000

AMRK023

AMRK024

AMpurE Animal Tissue Genomic DNA Mini


• 100 mg of Tissue sample, Upto ~10 µg high quality DNA


25 Isolations

50 Isolations

4,000

7,250

AMRK027

AMRK028

Gel DNA Extraction Spin

• Spin Column based fast protocol

• 70 bp to 16 kb fragments eluted

• ~ 70-80% recovery

50 Extractions

100 Extractions

4,500

8,000

AMRK025 AMpurE Crude DNA Universal


• Any crude DNA preps re-purified

• DNA suitable for all downstream applications (PCR, digestions, ligations etc.

50 Isolations 5,500

AMRK026 AMpurE DNA from Swab


• DNA quantity good enough for 10 or more PCR reactions

50 Isolations 8,500

AMRK029

AMRK030

AMRK031

AMpreP Plasmid DNA Mini

• Solution based quick protocol

• 1-5 ml culture, Upto ~20 µg good quality DNA

50 Isolations

100 Isolations

200 Isolations

2,200

4,250

8,000




14

AMRK032

AMRK033

AMpreP Plasmid DNA Midi


• 50- 100 ml culture, Upto ~500 µg good quality DNA

20 Isolations

50 Isolations

2,750

5,000

AMRK034

AMRK035

AMpreP Plasmid DNA Maxi


• 100ml or more culture and upto 1mg good quality DNA

10 Isolations

25 Isolations

2,500

4,800

AMRK036

AMRK037

AMpreP Plant Genomic DNA Mini


• 100 mg Leaf or soft tissue, Upto ~20 µg good quality DNA

50 Isolations

100 Isolations

2,200

4,250

AMRK038

AMRK039

AMpreP Plant Genomic DNA Midi


• 500 mg- 1 gm Leaf or soft tissue, Upto ~500 µg good quality DNA

20 Isolations

50 Isolations

2,750

5,000

AMRK040

AMRK041

AMpreP Plant Genomic DNA Maxi


• 1-2 gm Leaf or soft tissue, Upto ~ 1 mg good quality DNA

10 Isolations

25 Isolations

2,500

4,800

AMRK042

AMRK043

AMpreP Bacterial Genomic DNA Mini


• 1-5 ml culture, Upto ~20 µg good quality DNA

50 Isolations

100 Isolations

2,250

4,200

AMRK044

AMRK045

AMpreP Fresh Blood Genomic DNA Mini


• 200 µl Blood(Unclotted Blood), Upto~10 µg good quality DNA

50 Isolations

100 Isolations

3,000

5,500

AMRK046

AMRK047

AMpreP Animal Tissue Genomic DNA Mini


• 100 mg of Tissue sample, Upto ~10 µg high quality DNA

50 Isolations

100 Isolations

4,000

7,500




15

AMRK048

AB5α Competent Cells

• 65x10 Transformation efficiency; shipment on Dry-Ice

• Can be stored at -70°C for 2-3 weeks

• -20°C storage not recommended

• Usage of 5-10 µl ligation mix for better results

50 µl X 10 nos. 5,000

AMRK049 50 µl X 20 nos. 9,500

AMRK050Protein SilverStaining Kit

• Highly sensitive staining kit

• Detects ~0.5 to 5 ng protein

• 2 hrs protocol

10 Staining 6,500

AMRK051 25 Staining 11,500

AMRK052Protein

Zn-Staining Kit

• Highly sensitive staining kit

• Detects ~ 10 ng protein

• 30 min protocol

10 Staining 5,500




16

AMBS014 5 x 1 ml 1,600Blue DNA Gel Loading Buffer (6X)

Nuclease free, Ready-to-use, Blue Dye

AMBS015 5 x 1 ml 1,600Orange DNA Gel Loading Buffer (6X)

Nuclease free, Ready-to-use, Orange Dye

AMBS016 5 x 1 ml 1,600TriCol DNA Gel Loading Buffer (6X)

Nuclease free, Ready-to-use, Both Blue & Orange Dye together

AMBS017 100 gm 2,300Guanidine Hydrochloride

Powder; Molecular Biology Grade

AMBS018 100 gm 5,000Guanidium Thiocyanate

Powder; Molecular Biology Grade

AMBS019 1 gm 2,800IPTG Powder; Molecular Biology Grade

AMBS020 5 x 1 ml 7,500X-Gal 100 mg/ ml, Ready-to-use

AMBS021 5 x 1 ml 1,800Lysozyme 10 mg/ml Solution, Low Nuclease

AMBS022 250 ml 3,500MOPS Buffer (10X) Nuclease free, Ready-to-use

AMBS023 5 x 1 ml 2,800PMSF (0.1M) Protease free, Ready-to-use

AMBS024 1 gm 44,800Proteinase K Powder; Molecular Biology Grade

AMBS025 100 mg 5,000Proteinase K Powder; Molecular Biology Grade

AMBS026 5 x 1 ml 3,000Proteinase K 10 mg/ ml; Protease free

AMBS027 1 ml 1,800Ribonuclease A 10 mg/ ml; DNAse free

AMBS028 500 mg 11,000Ribonuclease A Powder; Molecular Biology Grade


Biosolutions Amnion BioSciences | 2011-12


17

AMBS029 100 ml 5,000AM RNAwipe Solution to wipe tables/ apparatus/ plastics/ Glassware to make RNAse-free

AMBS030 4X1 ml 6,000RNA Loading Buffer (5X)

RNAse free; Ready-to-use

AMBS031

AMBS032 AM RNAstable

• Stabilizes and protects RNA from degradation

• Aqueous, nontoxic cell & tissue storage reagent

50 ml

250 ml

4,000

16,000

AMBS033 100 ml 2,000Sodium Acetate (3M) Nuclease free; Ready-to-use

AMBS034 100 ml 1,000SDS Solution (10%) Nuclease free; Ready-to-use

AMBS035 100 ml 1,600SDS-PAGE Stacking Ready-Mix

5%; Just add APS and TEMED to cast PAGE

AMBS036 100 ml 2,200SDS-PAGE Separating Ready-Mix






AMBS039 10 x 100 mg 1,800Ammonium Persulphate

Analytical Reagent Grade

AMBS040 10 x 1 ml 4,000TEMED Analytical Reagent Grade

AMBS041 100 ml 2,200Acrylamide Mix (30%) In 19:1 ratio

AMBS042 100 ml 2,200Acrylamide Mix (30%) In 29:1 ratio

AMBS043

AMBS044

100 ml

5 x 2 ml

2,200

2,000

Acrylamide Mix (30%)

Protein Loading Buffer (5X)

In 36.5:1 ratio

Ready-to-use




18

AMBS045 1000 ml 2,750Tris Glycine SDS Buffer (10X)

Ready-to-use

AMBS046 1 M pH8, Mol Bio grade, Nuclease free 500 ml 5,000

Tris-CLAMBS047 1.5 M pH8.8, Mol Bio grade, Nuclease free 500 ml 5,000

AMBS048 1 M pH6.8, Mol Bio grade, Nuclease free 500 ml 5,000

AMBS054 5 x 100 ml 5,000Water

AMBS055

Protease, Nuclease free, Molecular Biology Grade

100 ml 1,600

AMBS049 200 ml 2,200TAE (50X) Nuclease free, Molecular Biology Grade

AMBS050 200 ml 1,600TBE (10X) Nuclease free, Molecular Biology Grade

AMBS051 100 ml 2,000TE (10X) Nuclease free, Molecular Biology Grade

AMBS052 100 ml 1,600Tween-20 DNAse free, Molecular Biology Grade

AMBS056 5 x 100 ml 7,000Water

AMBS057

DEPC- treated, RNAse free, Molecular Biology Grade

100 ml 1,800

AMBS059 10 Loadings 1,400Protein Molecular weight MarkerAMBS060

8 bands, 110 kDa, 97 kDa, 66 kDa, 51 kDa, 30 kDa, 25 kDA, 18 kDa, 14 kDa

50 Loadings 5,500

50 gmAMBS058 7,250BSA (Blotting) Protease-free; For Blocking of membranes inWestern

25 LoadingsAMBS061 3,500Protein Marker for Silver Staining

Concentration normalized for optimum detection

AMBS053 100 gm 1,600Urea Powder; Molecular Biology Grade




19

Terms & Conditions

Payment TermsThe payment of invoice shall be paid by customer within the time period or in advance as per applicable or in manner as agreed with the customer.Maximum Credit limit for any product :30DaysTaxes:- Excise Duty: Our prices are inclusive of Excise duty, Education cess.- Central Sales Tax: Will be charged as applicable. - Local Sales Tax / VAT: Extra as applicable. - Octroi and other levies, if any will be to the buyers account.- Service Tax: Will be charged extra as applicable for custom services.- If You wish to avail any concession on tax must provide necessary concession Forms (Form C/D). In case of non

availability of any Forms, full amount of sales tax will be charged.

Price quoted in this catalogue are ex-stock pricesPacking, Forwarding and Freight charges will be extra ourside BangalorePrices are valid until 31st March 2012 Rate Contract: To avail Rate Contract facility, please contact us.

Disputes: All disputes are subject to Bangalore Jurisdiction only.

DELIVERY POLICY

Perishable Items: Delivery charges will be added to the price of each order. Please specify shipping instructions in your order, so that we can try to accommodate your request. On the other hand we will decide the best mode of shipment based on the nature of the material and destination.

WarrantyWarranty period for all Amnion Biosciences products is limited.Ensure that the products perform as per specification indicated in the Data Sheet. In case of nonperformance, the products will be replaced free of charge. Warranty is not applicable for the products beyond expiry date and for storage conditions as stated in the data sheet.In case of non-performance please report us within 48 hours of delivery

Terms of SaleAmnion BioSciences and its authorized dealers/ representatives would adhere to the expressed or written terms of sale with the customer from case to case basis.

Legal Terms

Trade Mark / Service MarkFicoll® is a registered trademark of Pharmacia Biotech AB.Tween ® is a registered trademark of ICI Americas, Inc.

Patents/Disclaimer: Some products/process mentioned in this catalogue/pricelist may be covered by patents issued and applicable in certain countries for certain periods. This is because, purchase of our products does not possess license to perform patented applications. Hence users have to ensure they have necessary license to use.

Disputes: All disputes subject to Indian Jurisdiction at Bangalore.

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