Наноробот коригира дефект в еритроцитната мембрана
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Slide 1
. - ,
IP3, NAADP, cADPr .
5Courtesy of DOE: www.nano.gov/html/facts/The_scale_of_things.html
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Sizing-Down
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(340/380)
CCD (Roper)(Sutter) (Eppendorf) (Eppendorf)Perfusion System
IP3- Ca+2 LyERIP3Ca2+IP3 inositol triphosphate
Traces = average 6 cells +/- s.e.m
cADPr- Ca+2 LyERcADPrCa2+cADPr - cyclic adenosine diphosphate ribose cADPr . Ry . ER cADPr Ry .Traces = average 6 cells +/- s.e.m
= :
: ( , 25nm) (, 8nm) (10nm)
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(ER)
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:IP3 Inositol triphosphatecADPr Cyclic adenosine diphosphate riboseNAADP Nicotinic acid adenine dinucleotide phosphate
: (ER) IP3 and cADPr (Ly) NAADP** .
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150mM K+4mM K+0.1M Ca++2mM Ca++20mM Na+145mM Na+4mM Cl-110mM Cl-IntracellularExtracellular ,
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Plate of cellsCellsIntroduce virusVirusInfected cells37 ()
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Submicron Diameter - Fluid insideCell MembraneINTRACELLULAREXTRACELLULAR - , , , ,
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Submicron Diameter :
Cylindrical NanochannelFluid insideCell MembraneINTRACELLULAREXTRACELLULARNanochannel connected to larger probe
PinjectionFlowLDP2P1FlowP1 > P2
(CNTs)
Carbon Nanotubes
Single Wall (SWCNT)Multi Wall(MWCNT)SWCNT 0.6 1.8 nm, 20 nm 500 mm, ,
MWCNT - >0.6 nm, 20 nm o 500+ mm, ,
, MWCNT10 nm (>50nm)
Narishige Piston Injector Eppendorf Femtojet Injector 6000 hPa :
AFM
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Electrical ConnectionQuartz ExteriorInnerCarbon FilmExposed Carbon Tip1 cm
Carbon TipQuartz Micropipette5 m
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25 m
Scale = 200 m
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