| enzyme experience energetic delivery · iepox toolbox >230 enzymes cambrex has developed and...
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| Enzyme experience Energetic delivery
The biocatalysis expertsyou’ll enjoy working with
Maria BohlinAnalytical ChemistR&D
InnovationClients from all sectors challenge us to identify enzymes suitable for the requested chemical conversions and develop the reaction ready for the integration into the complete production workflow. We respond by creating patentable know-how they can own, whilst we can operate as their reliable bulk supplier.
Adopting our technology requires no specialist equipment or knowledge, which keeps our clients’ processes simple and their costs down.
ExperienceSince 1999, we’ve grown to specialize in developing processes, designing and optimizing routes and providing R&D screening services using our proprietary library of >600 enzymes.
Our services in this area include:
• Enzyme screening
• Enzyme development
- Cloning and gene discovery
- Characterization and description of genes for different enzymes and functions
- Using different expression systems
• Process development and enzyme manufacturing
- Ability to work with different organisms and enzymes
- Process know-how for industrial enzyme production
- Process optimization and technology transfer available
• Synthetic route development and consulting
• Analytical method development
PerformanceFrom bulk production to industrialization, our specialists offer your business more benefits:
• Shorter synthetic and alternate routes
• Reagents and solvents are biodegradable
• Typical side reactions are avoided, meaning few by-products and impurities
• High conversion, yield and enantiomeric excess
• Low enzyme and negligible cofactor costs
• Biocatalyst experts you’ll enjoy working with
Read on to learn more about the conversions and reaction parameters Cambrex can achieve for you…
We’re big on biocatalysis
• Substrate load 100-400g/l
• Simple technology and equipment/standard batch reaction
• Inexpensive enzymes for R and S alcohols available in any scale
• Excellent enantio purity (>99.8%)
• Complete and quick conversion in 6-12 hours
• Cost contribution of coenzyme NAD(P) <1€/kg product
• No expensive ingredients or solvents
• Easy work up and removal of enzyme
• Environmentally friendly
Core TechnologyKetoreductases (>230 enzymes)
Core TechnologyKetoreductases for multiple applications
2 3
R1 R2
high value chiral alcohol
OH
R1 R2
O
NAD(P)H
IEPox
NAD(P)
OHO
Small and halogenated compounds
CICI
CIF
FF
OH
OHOH
OH
Chiral Intermediates for API
O
OH
OH
OOH
SN
OH
OH
OCI
OH OH O
O
CN
OH OH O
OCI
OH
OEt
O
Statine side chains
OH OH
COOH
CH3
Steroids and Bile acids OH
O N
OHOH
O O
S S
Cyclic compounds
Diols
OH
OH
OH
OH
OH
O
CI
Complex and bulky compounds
NHO
Adam LanebjerAnalytical Chemist, QC
Quality
IEPox Toolbox>230 enzymes
Cambrex has developed and commercially manufactured APIs, intermediates and bulk chemicals using biocatalysis for over 15 years. As a result, our enzymes and processes are improving cost and the efficiency of manufacturing small molecules at multi-ton scale. Not just in pharmaceuticals and generics, but in everyday life – from cosmetics and fragrances to food flavorings.
• For conversion of racemic amines to chiral amines or access to keto compounds
Monoamine - or amino acid oxidases
• Substrate load 50-150g/l
• Simple technology and equipment/standard batch reaction
• Inexpensive enzymes for R and S amines available in any scale
• Excellent enantio purity (>99.8%)
• >90% conversion in 6-12 hours
• No expensive ingredients or solvents
• Easy work up and removal of enzyme
• Environmentally friendly
Core TechnologyTransaminases (>100 enzymes)
Special Biotransformations
Core TechnologyTransaminases for multiple applications
4 5
R1 R2
high value chiral amine
NH2
R1 R2
O
O
IEPAT
or Alanine
or Pyrovate
NH2
Aliphatic chiral amine
NH2
Amphetamine
NH2R
Aromatic amine
NH2
Cl
NH2R
NH2
OH
OR1
NH2
OHR1
NH2
OR
O
Alpha and beta amino acids / esters amino alcohols
O NH2
Cyclic compounds
NH2
NH
N
NH
Heterocyclic amine
R RO
OH
C N
NH2
H2O
C Nitrilase
R RO
NH2
C N
H2O
C Nitrile hydratase
RROO
OHNH2
H2O
CC Amidase
R R
NADPH
NADP
NO2 NH2 Nitroreductase
NH2O
NH2
R
O
OHR
O
O
OHH2O
H2O2NH3
DAAO SaaT
NH2
OH
Cl O
DL-2-chlorophenylglycine
OH
Cl O
O
2-(chlorophenyl)acetic acid
NH2
OH
Cl O
L-2-chlorophenylglycine
Johan MattssonAnalytical Chemist
R&D
Anna GranbergAnalytical Chemist, QC
Quality
IEPox Toolbox>100 enzymes
Example
Amino acid oxidase/Monoamine oxidase
Nitrilases/Nitrile hydratases/Amidases/Nitroreductases
2
• Lactate oxidases
• Glycolate oxidases
• Azoreductases
• Halohydrin dehalogenases
• Aldolases (N-Acetylneuraminic acid lyase)
• ENE-Reductases
And many others
• Substrate concentration 50 up to 120g/l
• Simple technology and equipment
• Inexpensive enzyme, recombinant over-expressed (<10€/kg product)
• >95% conversion in 24 hours
• Cost contribution of co-enzyme <5€/kg product
• No expensive ingredients or solvents
• Easy work up and removal of enzyme
• Environmentally friendly
Delta1/Delta4 Dehydrogenation
Nitrilases
Esterases/Lipases and Hydrolases
6 7
CNS CN
CN
CN
OHCN
CNN
NOH
NO
CN
CN
O
O
HH
H
O
O
HH
H
Androsten-3,17 -dione H2O2 O2
IEPDD
acceptor
acceptor H2
H2OR2
R1 COOR3
R2
R1 COOR3
R1
COOR3
R1
COOH
R2
R1 COOH
R1
COOR3
+
+
Sibel AslanAnalytical Development Manager, R&D
Analytical Development
• Substrate concentration 100g/l
• Simple technology and equipment
• Inexpensive enzymes, recombinant over-expressed
• More than 95% conversion in 24 hours
• No additional acceptor required
• No expensive ingredients or solvents
• Easy work up and removal of enzyme
• Environmentally friendly
Oxidases
IEPNit Toolbox>15 enzymes
Broad substrate range
25 enzymes
NAD and NADP dependent Formate dehydrogenases (cofactor regeneration NADH and NADPH)
NADP and NAD dependent Glucose dehydrogenases (cofactor regeneration NADH and NADPH)
NADH oxidase
H2OR2
R1 COOR3
R2
R1 COOR3
R1
COOR3
R1
COOH
R2
R1 COOH
R1
COOR3
+
+
O
R OH
OH
O
R OH
OH
O
R OH
O
O2
H2O2
H2O2 O2
IEPLO
R1
OHR1 CHO
+
O
O
HH
H
O
O
HH
H
Androsten-3,17 -dione H2O2 O2
IEPDD
acceptor
acceptor H2
2H2O2 H2O+O2
NAD(P)+ NAD(P)H
+
Formate
H2CO3 H2O CO2
H2O
NAD(P)+GDH NAD(P)H
Glucose
Gluconolactone Gluconic acidH2O
NADH + H+ + O2 NAD + H2O2
IEPNitToolbox> 15 enzymes
Broad substrate range
CNS CN
CN
CN
OHCN
CNN
NOH
NO
CN
CN
H2OR2
R1 COOR3
R2
R1 COOR3
R1
COOR3
R1
COOH
R2
R1 COOH
R1
COOR3
+
+
O
R OH
OH
O
R OH
OH
O
R OH
O
O2
H2O2
H2O2 O2
IEPLO
R1
OHR1 CHO
+
O
O
HH
H
O
O
HH
H
Androsten-3,17 -dione H2O2 O2
IEPDD
acceptor
acceptor H2
2H2O2 H2O+O2
NAD(P)+ NAD(P)H
+
Formate
H2CO3 H2O CO2
H2O
NAD(P)+GDH NAD(P)H
Glucose
Gluconolactone Gluconic acidH2O
NADH + H+ + O2 NAD + H2O2
IEPNitToolbox> 15 enzymes
Broad substrate range
CNS CN
CN
CN
OHCN
CNN
NOH
NO
CN
CN
Peroxidases/Catalases
Contact CambrexExperts you’ll enjoy working withwww.cambrex.com
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