4 waf
Post on 05-Jan-2016
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B
A4 WAF3 WAF 5 WAF 6 WAF
3.0
2 WAF
pIkD205116
9784665545
36
2924
20
4.0 5.0 6.0 7.0 8.0 9.0 10.0
3 WAF 4 WAF 5 WAF 6 WAF2 WAF
4.0pI 5.0 6.0 7.0
205116978466
4555
36
2924
20
kD
Houston et al., 2009
Figure S1
Figure S1. Two dimensional reference maps of castor proteins isolated from seeds of 2, 3, 4, 5, and 6 WAF. Total protein was isolated from the five experimental stages of castor seed. Protein extracts (0.05 mg) were labeled with Cy5, separated by wide and narrow IPG strips, and fractionated by 2-DGE. A, Protein extracts were separated with pH 3-10, wide-range IPG strips. B, Proteins were fractionated using narrow-range IPG strips, pH range 4 to 7. Migrating positions of isoelectric point (pI) and molecular mass (kD) markers are at the top and left of the gels, respectively.
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