an employment of flow cytometry in the biosystematics of the genus galeobdolon
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An employment of flow cytometry in the biosystematics of the genus
Galeobdolon
An employment of flow cytometry in the biosystematics of the genus
Galeobdolon
Radka Rosenbaumová
Genus Genus GaleobdolonGaleobdolonGenus Genus GaleobdolonGaleobdolon
G. luteum G. flavidum
2n = 2x = 18
Genus Genus GaleobdolonGaleobdolonGenus Genus GaleobdolonGaleobdolon
G. montanumG. luteum
2x 4x
G. endtmannii
2n = 2x = 18
Genus Genus GaleobdolonGaleobdolonGenus Genus GaleobdolonGaleobdolonDistribution
-------G. montanum
........
G. flavidum
G. luteum
G. argentatum
G. endtmannii
Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation
Standard: Glycine max cv. Polanka, (2C = 2.5 pg)
0
100
200
300
400
500
0 250 500 750 1000
Standard4x
G. montanumG. argentatum
0
150
300
450
600
750
0 250 500 750 1000
Standard
2x
G. luteumG. flavidum
Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation
Triploids
500
400
300
200
100
00 50 100 150 200 250
2x3x
4x
Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation
Triploids
Ploidy level estimation Ploidy level estimation Ploidy level estimation Ploidy level estimation
Sympatric occurence
12%
G. luteumG. montanum G. argentatum
Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation
G. endtmannii
Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation
G. luteum
2x 4x
G. montanum
2x + 4x
4x
G. endtmannii
2x
Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination
Species 2C DNA content ± SE
(pg)
1C DNA content (pg / Mbp)
Genome size (pg)
G. luteum 3.27 ± 0.002 1.63 / 1597 1.63
„G. endtmannii“ 3.28 ± 0.02 1.64 / 1607 1.64
G. flavidum 3.41 ± 0.02 1.70 / 1666 1.70
G. montanum 6.33 ± 0.04 3.17 / 3107 1.58
G. argentatum 6.36 ± 0.04 3.18 / 3116 1.59
„G. montanum“ 6.59 ± 0.04 3.29 / 3224 1.65
„G. endtmannii“ 6.41 ± 0.03 3.20 / 3136 1.60
Triploids 4.85 ± 0.04 2.43 / 2381 1.62
Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination
1.622.43 / 23814.85 ± 0.04Triploids
1.603.20 / 31366.41 ± 0.03„G. endtmannii“
1.653.29 / 32246.59 ± 0.04„G. montanum“
1.593.18 / 31166.36 ± 0.04G. argentatum
1.583.17 / 31076.33 ± 0.04G. montanum
1.701.70 / 16663.41 ± 0.02G. flavidum
1.641.64 / 16073.28 ± 0.02„G. endtmannii“
1.631.63 / 15973.27 ± 0.002G. luteum
Genome size (pg)
1C DNA content (pg / Mbp)
2C DNA content ± SE
(pg)
Species
4.8
4.9
4.85
Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination
1.622.43 / 23814.85 ± 0.04Triploids
1.603.20 / 31366.41 ± 0.03„G. endtmannii“
1.653.29 / 32246.59 ± 0.04„G. montanum“
1.593.18 / 31166.36 ± 0.04G. argentatum
1.583.17 / 31076.33 ± 0.04G. montanum
1.701.70 / 16663.41 ± 0.02G. flavidum
1.641.64 / 16073.28 ± 0.02„G. endtmannii“
1.631.63 / 15973.27 ± 0.002G. luteum
Genome size (pg)
1C DNA content (pg / Mbp)
2C DNA content ± SE
(pg)
Species
Nuclear genome size determinationNuclear genome size determinationNuclear genome size determinationNuclear genome size determination
G. luteum / G. flavidum
0
250
500
750
1000
1250
0 250 500 750 1000
G.l.G.f.
Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination
1.622.43 / 23814.85 ± 0.04Triploids
1.603.20 / 31366.41 ± 0.03„G. endtmannii“
1.653.29 / 32246.59 ± 0.04„G. montanum“
1.593.18 / 31166.36 ± 0.04G. argentatum
1.583.17 / 31076.33 ± 0.04G. montanum
1.701.70 / 16663.41 ± 0.02G. flavidum
1.641.64 / 16073.28 ± 0.02„G. endtmannii“
1.631.63 / 15973.27 ± 0.002G. luteum
Genome size (pg)
1C DNA content (pg / Mbp)
2C DNA content ± SE
(pg)
Species
3.7 %
Nuclear genome size determinationNuclear genome size determinationNuclear genome size determinationNuclear genome size determination
G. argentatum X G. montanum
0
180
360
540
720
900
0 250 500 750 1000
„G.m“G.m.
Nuclear genome size determinationNuclear genome size determinationNuclear genome size determinationNuclear genome size determination
G. argentatum X G. montanum
ConclusionsConclusionsConclusionsConclusions
3x
Sympatric occurence
G. endtmannii
G. flavidum
G. montanum X G. argentatum
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