bloodborne pathogens edgar romo, rehs environmental health & biosafety specialist janette de la...
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Bloodborne Pathogens
Edgar Romo, REHSEnvironmental Health & Biosafety Specialist
Janette De La Rosa Ducut, Ed.D.Training Manager
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1. Access the Bloodborne Pathogens Standard2. Identify epidemiology and symptoms
3. Explain the modes of transmission4. Locate the Exposure Control Plan at UCR
5. Recognize tasks/activities
6. Identify methods of prevention
7. Decontaminate and dispose of PPE
Objectives
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8. Select proper PPE
9. Obtain, or decline, the Hep B vaccine10. Follow emergency procedures11. Take action in an exposure incident12. Complete post-exposure evaluation13. Recognize signs and labels
14. Manage biohazardous waste
15. Obtain answers to your questions
Objectives
4
Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Bloodborne Pathogens Standard
The StandardApply to all work where occupational exposure to bloodborne pathogens is reasonably anticipated View online at
http://ehs.ucr.edu/biosafety
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Bloodborne Pathogens Standard
Bloodborne PathogensMicroorganisms that are carried in blood
that can cause disease
HBV HIV
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Epidemiology and Symptoms
EpidemiologyPlan and evaluate strategies to prevent illness by studying how diseases occur.
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Modes of Transmission
Routes of exposure Ways toxins enter the body Inhalation Absorption
Injection Ingestion
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Modes of Transmission
Mucous Membranes
Injection Non-Intact Skin
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Modes of Transmission
Potentially infectious materialsTransmission of a biohazardous agent via body fluid
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Modes of Transmission
Potentially infectious materials
urinefeces
vomit
tears
sweat
sputum
nasal secretions
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Exposure Control Plan
Exposure Control Plan
Review online at http://ehs.ucr.edu/biosafety
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Exposure Control Plan
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Exposure Control Plan
Biological Use Authorization (BUA)Review the conditions / restrictions for use
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Tasks and Activities
Determine if your tasks, activities, or job description involves exposure and protect yourself.
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Tasks and Activities
Follow precautions when handling…Syringes, needles, or sharpsEmergency responseBody fluidsSpill clean-ups
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Tasks and Activities
Job DescriptionsPhysicians and NursesAthletic TrainersLifeguardsEmergency Responders (i.e., UCPD)
Laboratory workersPhlebotomistsBiohazardous waste workersCustodiansGroundskeepers
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Methods of Compliance
Methods ofCompliance
Universal PrecautionsHousekeepingEngineering ControlsWork PracticesPPE
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Methods of Compliance
Universal PrecautionsHandle as if items were known to be infectious
Wash hands and use protective barriers (i.e., PPE) for direct contact with blood and other potentially infectious materials.
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Methods of Compliance
HousekeepingDecontaminate with a 10% bleach solution(1 part bleach to 9 parts water) or other approved disinfectant for at least 15 minutes
Sanitize with 1:10 bleach solution
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Methods of Compliance
Engineering ControlsEliminate or reduce exposure through use or substitution of
machinery or equipment.
Dispose of sharps in a puncture-
proof container
Needleless System Use aerosols in a Biological Safety
Cabinet
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Methods of Compliance
Work Practices
Implement safe ways of performing your tasks
One-Hand Technique
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Methods of Compliance
HygieneAvoid contactCheck for open wounds Wash hands
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EatingDrinkingSmoking…in the laboratory
Methods of Compliance
NO
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Methods of Compliance
PPECheck for defects, remove properly, and replace immediately.
Do not reuse.
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
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PPESelection
PPE
Gloves: Latex or NitrileEyes: Safety Glasses,
Goggles or Face Shield
Body: Lab CoatFeet: Closed-Toe Shoes
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
PPEDecontamination and Disposal
After usingDecontaminate (i.e., wash hands after removing gloves)
Dispose of in biohazard containers
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Properly remove PPE
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Hepatitis B Vaccination
Hep B Vaccine
Participate in a free series of three shots (80-95% effective at preventing infection)
Hepatitis B Vaccination
Hep B Vaccine
You may elect to decline the vaccine
Hepatitis B Vaccination
Hep B Vaccine
Receive the vaccine, OR Complete the Declination Form
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Emergency Procedures
In Case of an AccidentObtain medical care and report the injury or illness
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Emergency Procedures
SpillsIsolate, decontaminate, and wash hands
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Emergency Procedures
Review the Emergency Procedures online at http://ehs.ucr.edu/emergency/procedures
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Exposures
ExposureContact between eyes, mouth, mucous membranes, skin cuts, or abrasions
Inju
ry R
epo
rt F
orm
Exposures
NotificationYou complete an Injury Report Form
UCR records exposures on a Sharps Injury Log.
Sh
arp
s In
jury
Lo
g
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Exposures
EmergencyProcedures
Wash skin immediately.Flush eyes with water for 15 minutes.Call 911 for help.Notify supervisor and EH&S.
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Post-ExposureEvaluation and Follow-Up
Medical EvaluationRoutes of exposureSource individualBlood testingProphylaxis (if applicable)
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Signs and Labels
Biohazard SymbolFlourescent orange or orange-red in color, with contrasting lettering
Signs and Labels
Affix biohazard label
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Waste
Biohazardous WasteBiologically contaminated waste that could potentially cause harm to human health, animal health, or the environment.
Examples: cell cultures, transgenic plants, recombinant DNA, and waste from production of bacteria / virus / fungi / parasites.
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Waste
LiquidWaste
The three types of biohazardous waste generated on campus are contaminated sharps, red bag waste, and liquid waste.
ContaminatedSharps
Red BagWaste
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Contaminated Sharps
WasteContaminated Sharps
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Label with the word “Biohazard” and the biohazardous symbol
WasteContaminated Sharps
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Store in approved sharps container
WasteContaminated Sharps
LabelsLabels
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Store until the container reaches the fill line.
WasteContaminated Sharps
Do not exceed fill line
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Decontamination is not necessary(unless you plan to dispose as physically hazardous waste)
WasteContaminated Sharps
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Dispose within the appropriate time after generation
WasteContaminated Sharps
Within 90 daysif stored below 0˚C
Within 7 daysif stored above 0˚C
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Submit a waste pickup request online by visiting www.ehs.ucr.edu and clicking on “Waste Pickup”
WasteContaminated Sharps
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WasteContaminated Sharps
Click on the link “Biohazardous or Radioactive Waste”
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WasteContaminated Sharps
Enter information and click on “Submit”
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WasteRed Bag Waste
Red Bag Waste
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Label with:1. “Biohazard” word2. Symbol3. Building
4. Room Number
WasteRed Bag Waste
Hinderaker 4108
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Label with:1. “Biohazard” word2. Symbol3. Building
4. Room Number
WasteRed Bag Waste
Hinderaker 4108
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WasteRed Bag Waste
Store in a labeled and leak-resistant secondary container
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WasteRed Bag Waste
Close containers when not in use.
Labels
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Treat within the appropriate time after generation by autoclaving
WasteRed Bag Waste
Within 90 daysif stored below 0˚C
Within 7 daysif stored above 0˚C
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Red Bag WasteWaste
Autoclave tape
Place indicator tape on the red bag
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Red Bag WasteWaste
Autoclave
Follow safe autoclave procedures
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Red Bag WasteWaste
Dispose of autoclaved waste in the building dumpster.
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Red Bag WasteWaste
Submit a waste pickup request online when autoclaving is not possible
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Go to www.ehs.ucr.edu and click on “Waste Pickup”
WasteContaminated Sharps
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WasteContaminated Sharps
Click on the link “Biohazardous or Radioactive Waste”
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WasteContaminated Sharps
Enter information and click on “Submit”
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LiquidWaste
WasteLiquid Waste
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Label with:1. “Biohazard” word2. Symbol
WasteLiquid Waste
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WasteLiquid Waste
Store in a clear, non-breakable, container with positive closure
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Treat within the appropriate time after generation by disinfecting
WasteLiquid Waste
Within 90 daysif stored below 0˚C
Within 7 daysif stored above 0˚C
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Mix 1 part bleach to 9 parts water
WasteLiquid Waste
10
9
8
7
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5
4
3
2
1
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Add to liquid waste and wait for 30 minutes
WasteLiquid Waste
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Pour down drain(only if decontaminated)
WasteLiquid Waste
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Waste
Mixed waste must be treated in the following order: 1. Radioactive 2. Chemical 3. Biohazardous
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Waste
Mixed WasteWaste that contains radioactive, chemical, and biohazardous components
32P
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Waste
Mixed WasteWaste that contains radioactive and biohazardous components32P
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Waste
Mixed WasteWaste that contains chemical and biohazardous components.
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Topics
1. Bloodborne Pathogens Standard
2. Epidemiology and Symptoms
3. Modes of Transmission4. Exposure Control Plan5. Tasks and Activities6. Methods of
Compliance7. PPE
(Selection)
8. PPE (Decontamination and Disposal)
9. Hepatitis B Vaccination10. Emergency Procedures11. Exposures12. Post-Exposure
Evaluation 13. Signs and Labels14. Waste15. Questions and Answers
Questions and Answers
Biosafety Officer
Email ehs@ucr.edu Call (951) 827-5528Visit http://ehs.ucr.edu/biosafety
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1. Access the Bloodborne Pathogens Standard2. Identify epidemiology and symptoms
3. Explain the modes of transmission4. Locate the Exposure Control Plan at UCR
5. Recognize tasks/activities
6. Identify methods of prevention
7. Decontaminate and dispose of PPE
Summary
116
8. Select proper PPE
9. Obtain, or decline, the Hep B vaccine10. Follow emergency procedures11. Take action in an exposure incident12. Complete post-exposure evaluation13. Recognize signs and labels
14. Manage biohazardous waste
15. Obtain answers to your questions
Objectives
117
For more information
ehs.ucr.edu
951 827 5528
ehs@ucr.edu
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