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In this guide:

UniProt (Universal Protein Resource): A comprehensive, high-quality and freely accessible resource of protein sequence and functional information

Sequence repositories

Genomic/Proteomic Research

Large-scale Small-scale

Sharing and using data:

Use/organise

the data

Analyse/re-

analyse the

data

Compare/integrate

with other data sets

Data source in UniProt:

Direct submissions

Gene name

Protein name

PRKN

BRCA1

DONSON

HedgehogDystrophin

Fidgetin

Keywords

Calcium

Antibiotic

DigestionSpecies

Mus musculus

Caenorhabditis elegans

Arabidopsis thaliana

GO term

Kinase

activity

oviposition

lysosome

Disease

Alzheimer

Down

syndrome

Malaria

ATG12

ATG12

ATG5

ATG5

ATG12

ATG5ATG16

ATG16

• Search for the gene names individually using the UniProtKB free text

search bar

• Use the advanced search to search by organism

Under the search bar on the

homepage

Use the retrieve

tool to search

for multiple

entries at a time

List your gene names

Select your parameters

29 entries match the

three gene names

3 entries are curator reviewed

26 entries have not been

reviewed by a curator

O94817

O94817

Q9H1Y0

Q9H1Y0

O94817

Q9H1Y0Q676U5

Q676U5

• Search UniProt using identifiers

• Find corresponding genes and protein entries

Use the ID mapping tool to convert UniProt

identifiers (accession numbers) to gene

names

Convert non-UniProt identifiers to UniProt

accession numbers and vice versa

Q9H1Y0

ATG12

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VPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEEL? =

Use Peptide search tool to find entries containing sequence

Use the Peptide search

tool to search for entries

containing a specific

string of amino acids

Enter peptide

sequence

Specify the organism

ATG12

ATG12

ATG5

ATG5

ATG12

ATG5ATG16

ATG16

Find:

The function of each

component with

evidence source and

publications listed

Find:

Binary one-to-one

interaction and

complex data

Q9H1Y0

ATG12

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VPNRHEISPGHDGTWNDNQLQEMAQLRIKHQEEL? =

Use Blast and Align to find similar proteins within the same

species and in different species

Search for similar

sequences using the

BLAST tool.

You can search for full-

length protein

sequences or small

sections of protein

sequence

The identity column

shows how similar your

search query is to

individual BLAST hits

in the results table

BLAST hits can be filtered

and individual hits can be

selected for further

analysis or added to the

basket

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To indicate functional, structural and evolutionary relationships

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Contact us: help@uniprot.org

Clicking on the number of

proteins will display all

the protein records in

UniProtKB

Download all proteome

sequence data as a fasta

file for comparison with

raw sequence data

Search for specific diseases using the

diseases tab on the homepage

Phenotypes as a result of

single/double/multiple mutations

Phenotypes as a result of RNAi,

indicating developmental stage if

applicable

Provide name of mutant if

known

Indicate if phenotype is due to

mutations of several amino

acids

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Contact us: help@uniprot.org

PIs: Alex Bateman, Alan Bridge, Cathy Wu

Key staff: Cecilia Arighi (Curation), Lionel Breuza (Curation), Elisabeth Coudert (Curation), Hongzhan Huang

(Development), Damien Lieberherr (Curation), Michele Magrane (Curation), Maria Martin (Development), Peter McGarvey

(Content), Darren Natale (Content), Sandra Orchard (Content), Ivo Pedruzzi (Curation), Sylvain Poux (Curation), Manuela

Pruess (Coordination), Shriya Raj (Coordination), Nicole Redaschi (Development)

Content / Curation: Lucila Aimo, Ghislaine Argoud-Puy, Andrea Auchincloss, Kristian Axelsen, Emmanuel Boutet, Emily

Bowler, Ramona Britto, Hema Bye-A-Jee, Cristina Casals-Casas, Anne Estreicher, Livia Famiglietti, Marc Feuermann,

John S. Garavelli, Penelope Garmiri, George Georghiou, Arnaud Gos, Nadine Gruaz, Emma Hatton-Ellis, Chantal Hulo,

Nevila Hyka-Nouspikel, Florence Jungo, Kati Laiho, Philippe Lemercier, Yvonne Lussi, Alistair MacDougall, Patrick

Masson, Anne Morgat, Sandrine Pilbout, Catherine Rivoire, Karen Ross, Christian Sigrist, Elena Speretta, Shyamala

Sundaram, Nidhi Tyagi, C. R. Vinayaka, Qinghua Wang, Kate Warner, Lai-Su Yeh, Rossana Zaru

Development: Shadab Ahmed, Emanuele Alpi, Leslie Arminski, Parit Bansal, Delphine Baratin, Teresa Batista Neto,

Jerven Bolleman, Borisas Bursteinas, Chuming Chen, Yongxing Chen, Beatrice Cuche, Edouard De Castro, Tunca Dogan,

Elisabeth Gasteiger, Sebastien Gehant, Leonardo Gonzales, Alexandr Ignatchenko, Giuseppe Insana, Rizwan Ishtiaq,

Vishal Joshi, Dushyanth Jyothi, Arnaud Kerhornou, Thierry Lombardot, Jie Luo, Mahdi Mahmoudy, Andrew Nightingale,

Joseph Onwubiko, Monica Pozzato, Sangya Pundir, Guoying Qi, Daniel Rice, Rabie Saidi, Edward Turner, Preethi

Vasudev, Yuqi Wang, Xavier Watkins, Hermann Zellner, Jian Zhang

European Bioinformatics Institute

(EMBL-EBI), Hinxton, Cambridge, UK

SIB Swiss Institute of Bioinformatics

(SIB), Geneva, Switzerland

UniProt Team

Protein Information Resource (PIR),

Washington DC and Delaware, USA

See the full list of upcoming webinars at

https://www.ebi.ac.uk/training/webinars

Please fill in the survey that launches after the webinar – thanks!