classic paper-2010 for web_0
Post on 07-Apr-2018
221 Views
Preview:
TRANSCRIPT
-
8/3/2019 Classic Paper-2010 for Web_0
1/57
Classic papers in biochemistry
Structure at 2.8 resolution of F1-ATPase frombovine heart mitochondria
Nature, 370, 1994, 621-628
Abrahams, Leslie, Lutter & Walker
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
2/57
Mitochondria and F-ATPase
1980-1994
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
3/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
4/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
5/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
6/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
7/57
How is pmf coupled to ATP synthesis?
Direct (Mitchell, Williams)
Indirect (Boyer, others)
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
8/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
9/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
10/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
11/57
2NADH H2
O
2NAD+
FADH
2
FAD 2 H
+
4 H+
4 H+
2 H
4 H+
4 H+
QQH
2
QQH
2
[O
]2
e
2e
2
e
2
e
2
e
3-4 H+
3-4 H+
ATP
ADP + Pi
Cyt c
HumanmtDNA
3 proteins
The thirteen proteins encoded in
mitochondrial DNA
7 proteins2 proteins
1 protein
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
12/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
13/57
P-loop sequences in F1-ATPase
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
14/57
P-loop sequences in F1-ATPase
consensus G X X X X G K T
169-1 G D R Q T G K T159-163 G G A G V G K T
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
15/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
16/57
Paul Boyer
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
17/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
18/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
19/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
20/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
21/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
22/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
23/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
24/57Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
25/57
ATP binding site ofTP
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
26/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
27/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
28/57
-
8/3/2019 Classic Paper-2010 for Web_0
29/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
30/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
31/57
The ATP synthase
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
32/57
20 nm
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
33/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
34/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
35/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
36/57
Generation of rotation
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
37/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
38/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
39/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
40/57
The H+/ATP and Na+/ATP ratios
For the synthesis of each ATP, the -subunit rotatesthrough 120
12 c-subunits/ring
H+
/ATP = 4 10 c-subunits in S. cerevisiae H+/ATP = 3.3 14 c-subunits in chloroplasts H+/ATP = 4.7 11 c-subunits in Na
+
-motive F-ATPases
Na+
/ATP = 3.7
Estimates of H+/ATP are in the range 3-4
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
41/57
The mammalian c-ring
The bioenergetic cost of making an ATP molecule
in mammalian mitochondria
Watt et al (2010) PNAS 107, 16823
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
42/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
43/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
44/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
45/57
Thursday, 2 December 2010
/O i i i i i
-
8/3/2019 Classic Paper-2010 for Web_0
46/57
P/O ratio in mammalian mitochondria
NADH 2e- to O2 =10 H+ pumped out
Succinate 2e- to O2 = 6 H+ pumped out
ADP/ATP exchange + Pi/H+
symport = 1 H+
in
ATP synthase H+/ATP = 8/3 = 2.7
ATP synthesis H+/ATP = 3.7
Moles ADP phosphorylated/2e- to O2 = P/O ratio
e- donor P/O calc P/O exp
NADH 10/3.7=2.7 2.5
Succinate 6/3.7=1.6 1.5
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
47/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
48/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
49/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
50/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
51/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
52/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
53/57
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
54/57
Species known to have trimethyl-lysine-43
Man, cows, sheep, pigs, rabbit, mice, rats
Chickens
Flies (Diptera)
NB Yeast has K43, but not trimethylated
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
55/57
17
Lindsay Baker, Mike Runswick, John Walker and John Rubinstein, unpublished work
Thursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
56/57
Lindsay Baker, Mike Runswick, John Walker and John Rubinstein, unpublished workThursday, 2 December 2010
-
8/3/2019 Classic Paper-2010 for Web_0
57/57
Unresolved issues
The a-c-ring interface
Understanding differences between F-ATPases(regulation, ring symmetries, peripheral stalks)
Reconciling structures and biophysics
top related