diagnostic microbiology and immunology
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Diagnostic Microbiology and Immunology
Chapter 31 Diagnostic Microbiology and Immunology Lab Diagnostic
Time Lines The Swab Clicker Question: Heres Some, Depending on
Specimen
Clicker Question Urine Analysis Dip Sticks
Top: After reaction with metabolites in urine. Bottom:Before
reaction with metabolites in urine Test time = 60 sec. Measures:
glucose, bilirubin, ketones, sp. gravity, pH, protein,
urobilinogen, nitrite, esterases.Top two right are false positives
to show the color reaction. Eosin Methylene Blue Agar There are
hundreds of Selective and Differential Bacteriological Media
Text goes on describing particular samples:wounds, feces, genital
swabs, etc. We will cover those when we get to specific diseases.
Urethral Swab: Microscopy + Plating on Thayer-Martin Agar
Oxidase Test Reagent poured onto the agar AFTER the colonies grew
up. Gas Pack, Anaerobic Chamber Some Clinical Tests Metabolic
Reactions The Classic API-20e
Glucose Phenol Red with Durham tube. TSI: triple sugar iron agar.
Citrate Test More Clinical Tests Testing Antibiotic
Sensitivity
Kirby-Bauer Zone of Inhibition E-test. MIC: Minimal Inhibitory
Concentration Some Antibiotics Interpretation of Size of the Zone
of Inhibition Pseudomonas aeruginosa MICs
Each numbered row one antibiotic, highest conc at left, lowest at
right. Clicker Question: E test Clicker Question: Working in a
BSL-4 Lab The Gen Micro Labs in AHC2 are BSL-1, most our research
labs are BSL-2. BSL-3 labs:to contain pathogens that could be
infective as an aerosol. BSL-1: lab people wear lab coats,
gloves..and usually does not involve pathogens. BSL-2: needs to
contain pathogens in hoods, etc.Aerosol generating equipment must
be in a biosafety cabinet. Disease Progression: Untreated Typhoid
Fever
Antibody Titers:Generally done by serial 1:2 dilutions from a blood
sample.Highest dilution that brings about an Ab-Ag reaction.
Clicker Question: Disease Progression: Untreated Typhoid
Fever
Antibody Titers:Generally done by serial 1:2 dilutions from a blood
sample.Highest dilution that brings about an Ab-Ag reaction.
Clicker Question Monoclonal Antibodies Precipitin Reaction
Precipitin Rxns on a Glass Slide
Clicker Question: Agglutination Reactions Latex Bead Agglutination
for S. aureus Fluorescent Antibodies
Clostridium septicum stained with FITC-Ab, C. chauvoel stained with
Rhodamine-Ab Direct and Sandwich (Indirect) Yellow-Green cells are
CD8 Cells Red-orange cells are CD-4 Cells
B and T Cells Yellow-Green cells are CD8 CellsRed-orange cells are
CD-4 Cells Cell Sorting Clicker Question: Enzyme Immuno-Assays EIA
Kits for Small Clinics Immunoblots: Detecting Blotted Proteins
(after electrophoresis) Nucleic Acid Diagnostics PCR Detecting
Mycobacteium tuberculosis
Lanes Molecular Weight Standards Lanes 2-12 from different TB
patients sputum Lanes from non-TB patients with lung symptoms. Lane
15 from known Mycobacterium tuberculosisDNA.
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