dna fingerprinting

Prepared by : Ain Nur Syazwani Nurul Farah Alia Izatul Syafiqa Nur Maizatul Azwa Ellya Farida

Dna fingerprinting

I who have discovered the DNA fingerprinting.My name is Alec Jeffreys.....Do you know who I am?

WHAT IS DNA FINGERPRINTI

NG?

DNA fingerprinting is a procedure used to analyse a person’s unique collection of DNA fragments.

Ex: Dna from tissue of hair,saliva,blood or semen found at the scene can be compared to those of a suspect.

The Advantages

of Dna Fingerprinti

ng

DNA fingerprinting is more accurate than fingerprinting in the identification of individuals.DNA fingerprinting is more useful forensic tool than blood types because many people belong to the same blood typeIt provides more information on a criminal’s identity as it is more specific.Very small quantities of DNA are required to carry out a highly accurate test. Example : small amounts of dried blood or semen are sufficient to identify the owner.DNA sample last much longer than fingerprints. DNA samples that are very old (up to 25 million years) can still be used.To test compatibility of potential organ donors with patientsDNA evidence is much harder to clean up at a crime scene than evidence such as fingerprints

In agriculture DNA fingerprinting is used to know if a seed is really from the variety being claimed. Detect genetically modified organisms in agriculture. Useful in herbal origin authentication, and for the various claims of medical uses related to some of these plants.Identify and breed thoroughbred horses

DNA Fingerprinting - YouTube.FLV

STEPS IN PRODUCING

DNA FINGERPRINTI

NG

Proses bermula dengan sel darah atau sampel dari mana ia diekstrak dan kemudian dipecahkan

kepada serpihan.

Serpihan tersebut telah dipisahkan mengikut saiz oleh electrophorasis melalui gel agarosa. Setiap gel terdiri daripada salinan banyak serpihan DNA tertentu.Ia tidak kelihatan tetapi ia boleh dilihat melalui pewarnaan.

DNA band dipindahkan ke membran nilon.

probe DNA bergabung dengan DNA yang spesifik pada membran nilon

melalui probe DNA radioaktif.

Lebihan bahan dibuang meninggalkan jalur DNA

corak yang unik

Corak DNA radioaktif dipindahkan ke filem x-ray

dengan pendedahan langsung.Corak kelihatan

paduan adalah CAP JARI DNA.

The various types of Fingerpri

nts

DISADVANTAGES Seen as an infringement of civil liberties especially when DNA

profiles are stored from people who have nothing to do with a particular criminal offence.

Concerns that the finding of trace amounts of DNA can falsely implicate a person in a criminal investigation.

Concerns that the complexity of testing and data storage means errors could occur.

DNA profiles could be related to certain illnesses and charcter traits and this information would be used by insurance companies and other organisations to the disadvantage of the individual.

There is also 1 in 50 billion chance of two DNA sequence being similar.

Video time!!

!

DNA Fingerprinting - YouTube_2.FLVDNA Fingerprinting - YouTube_3.FLV

DNA Fingerprinting - YouTube_4.FLV

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