dna microarray. biology statistics (data analysis) engineering (microfluidic, microfabrication,...
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DNA microarray
BiologyStatistics(data analysis)
Engineering(microfluidic, microfabrication, robotic automation…)
Chemistry(surface modification)
Optics(signal detection)Electronics, physics
Research Integration
Gene Expression
Gene Discovery
Genotyping (SNPs)Drug Discovery
Diagnosis
Sequencing/resequencing
Disease Barcoding, Drug Susceptibility
Crop ImprovementBasic Research
Preparation of DNA solution for microarray
PCR amplification
PCR product purification
96-well plate
Quality control
Collections of ESTs, library clones...
DNA printing solution
384-well plate
Re-racking
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Printing of DNA microarray
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Each spot represents different gene/clone
Flowers
Leaves
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mRNA isolation
DNA microarray probe preparation
cDNA labeling with fluorescent dyes
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Flower
Leaf
DNA microarray hybridization
Modified from http://darwin.bio.uci.edu/~faculty/wagner/array2.html
Labeled DNA hybridizes to corresponding DNA/gene
Laser excitation
Cy5: ~650 nmCy3: ~550 nm
Image overlay
No changes
Flower up-regulated genes
Leaf up-regulated genes
Cy3 labeled cDNA from etiolated seedlingsCy5 labeled cDNA from green leaves
Arabdidopsis 11.5K microarray
Girke et al. 2000
Detection of cross hybridization
Gygi et al. 1999
Correlation between protein and mRNA levels
Plant functional genomics
Basic mechanism of plant growth and developmentBasic mechanism of plant growth and development
Application in agricultural biotechnologyApplication in agricultural biotechnology
增加農作物產量創造高營養價值之農作物創造具高適應力之農作物利用農作物作為生物反應器
DNA microarrayand beyond….
Insertion site identification from knockout population
Mahalingam and Fedoroff, PNAS (2001) 13, 7420-7425
Pollack et al. 1999
DNA copy number detection
Iyer et al. Nature (2001) 409,533-38
DNA-protein interaction
MacBeath et al. (2000) Science 289, 1760-3
Protein-protein interaction (protein microarray)
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