extracted from presentation given by robin todd at 2007 ent soc. america meeting icr, inc. robin...

Extracted from presentation given by Robin Todd at 2007

Ent Soc. America meetingICR, Inc.

Robin Todd, ICR, Inc.

CleanZone

• A non-chemical method that works

• Elevated ozone levels – 100 ppm +

• Ozone Generator

• Confined spaces

• Used for mold elimination

• Bed bugs a new target

Robin Todd, ICR, Inc.

Test

• CleanZone Systems LLC. Lisle, IL

• Conducted study in an empty home

• Showed efficacy

• Asked ICR for independent confirmatory test

Robin Todd, ICR, Inc.

Safety

• EPA’s acceptable level: 0.08 ppm for 8 hours (0.07- 0.075 ppm proposed)

• CleanZone generates 100ppm +

• Test run in green house – safety

• Ozone levels monitored

• CleanZone staff attended test and ran generator

Robin Todd, ICR, Inc.

Test details

• Test chamber built

• 8x18x7ft tall (1008 ft2)

• Plastic sheeting over PVC piping

• Sand snakes hold down plastic

Robin Todd, ICR, Inc.

Bed bug containment & Replication

• 10 containers with screened lids

• 10 containers covered with cloth

• 10 adult bed bugs per container

Robin Todd, ICR, Inc.

Test Chamber

Robin Todd, ICR, Inc.

Control Chamber

• 4x6x7 ft high chamber (168 ft2)

• In a different building – no elevated ozone

Robin Todd, ICR, Inc.

Other species tested

• German cockroaches

• Confused flour beetles

• Pharaoh ants

• American house dust mites

Robin Todd, ICR, Inc.

Containers of insects

Robin Todd, ICR, Inc.

Observations during exposure

• Ozone levels observed hourly

• One container of each species removed hourly

- Knockdown checked = monitors

• Self-sealing port – avoid ozone loss

Robin Todd, ICR, Inc.

Results

• Ozone in test chamber 118.3 ppm (mean)• Generator run for 22.7 hours• Peak (150.7 ppm) at 9 hours• Still air – underestimated levels• Ozone levels in control chamber: 0 – 0.1 ppm

Robin Todd, ICR, Inc.

Ozone levels

Ozone Levels in Test Chamber

020406080

100120140160

1 2 3 4 5 6 7 8 9 10 11

Hours

Ozo

ne

(pp

m)

Robin Todd, ICR, Inc.

Results

• 100% knockdown in monitor containers

for all species except ants

Robin Todd, ICR, Inc.

Results

• Test Chamber - 100% mortality +24 hours after removal from chamber

• 4% mortality in control

Robin Todd, ICR, Inc.

Hourly Knockdown

Bed Bug Knockdown in Monitor

0.00%20.00%40.00%60.00%80.00%

100.00%120.00%

1 2 3 4 5 6 7 8 9 10 11

Hours of Exposure

Open Containers Covered Containers

Robin Todd, ICR, Inc.

Dead bed bugs after treatmentno longer clinging to filter paper

Robin Todd, ICR, Inc.

Other Species

• Treatment killed 100% of roaches and dust mites

• 86% (open) 98% (covered) of beetles

• 40% (open) 83% (covered) of ants– Ants in covered nest containers

– because sheltered from ozone?

Robin Todd, ICR, Inc.

Acknowledgement

• John Sharpe, formerly with ICR, conducted this test. He did a great job.

Robin Todd, ICR, Inc.