final presentation - multivariate methodology assessment ......final presentation - multivariate...

Multivariate Methodology Assessment

Final Report on the Mission and Aransas Rivers

James TolanJames Tolan

Janet Nelson

TPWD – Coastal Fisheries

Scope of Work

• Physicochemical measurements

• Water and sediment quality

• Flow measurements

• Nekton and benthic sampling

• Instream and riparian habitat

classification

• Land cover classification

Biological

Physical Chemical

Assessment Methodology

• Multivariate, community-based

– Resemblance matrix

• Highly visual techniques • Highly visual techniques

– Cluster Analysis, MDS, PCA

• Analysis based on bootstrap permutations

– ANOSIM, SIMPER, RELATE

Biological

Physical Chemical

G

Theoretical MD S – Imp aired

Waterbody

Transform: Square root

Resemblance: S17 Bray Curtis similarity

Stream A

B

C

F

E

2D Stress: 0.1

High

G

H

Intermediate Exceptional

Geographic Setting

Segment 2001

- 1 permitte d discharger into the

Tida l Segment,

- 2 permitte d dischargers above

th e Tida l Segment

Missio n River

1 = Upper

2 = Middle

3 = Lower

AL U - Presume d High

Fully Supportin g for D.O.

Non-Supportin g for Rec.

Contact - Bacteria

ss on

Mission 1

Mi i 2Mission 2

Mission 3

Upstream Downstream

Segment 2003

- 4 permitte d dischargers

al l wel l Upstream

- 5 permitte d dischargers belo w the

Tida l Segment vi a Chiltipi n Creek

Aransas River

1 = Upper

2 = Middle

3 = Lower

AL U - Presume d High

Fully Supportin g for D.O.

Non-Supportin g for Rec.

Contact - Bacteria

ransas

Aransas 1

A 2Aransas 2

Aransas 3

Upstream Downstream

Wastewater Outfalls

Me

an

alin

ity

(PS

U)

M-A Study = Drought

Sa

linity (

PS

U)

30

40

50

60

Spring Summer Fall Spring Summer Fall

Me

an

S

0

10

20

Mission River (MR 1)

Aransas River (AR 1)

2008 2009

Water Colum n Profiles (Surface)

2

4 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

Avg DO mg/L

Secchi MR 1

MR 2

AR 3

2D Stress: 0

PCA Analysis MDS Means Plot

-4 -2 0 2 4

PC1

-4

-2

0

PC

2

Avg Temp

Avg pH

Avg Salinity

MR 3

AR 1

AR 2

DataS onde Deployments

MR 1

AR 2

MR 3

2D Stress: 0.01

2

4 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

PCA Analysis MDS Means Plot

MR 2

AR 1

AR 3

MR 3

-4 -2 0 2 4

PC1

-2

0

PC

2

Temp_C

DO mg/L

pH

Salinity

Sediment Composit ion (Middle)

4

6 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

Gravel

MR 1

MR 2

AR 2

AR 3

2D Stress: 0

PCA Analysis MDS Means Plot

-8 -6 -4 -2 0 2

PC1

-2

0

2

PC

2

Organic Carbon, Total

Percent MoistureSiltClay

Sand

MR 3

AR 1

TKN

W ater Quality Measurements

MR 1

MR 2

AR 1

2D Stress: 0

2

4

6 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

BOD

TSS VSS

Ammonia

TKN

Phosphorus

Chloride Orthophosphate

PCA Analysis MDS Means Plot

MR 3

AR 2

AR 3

-6 -4 -2 0 2 4

PC1

-4

-2

0

2

PC

2

BOD

Nitrate-N

Chloride Orthophosphate

W ater Quality Measurements

2

4

6 Orthophosphate

6E-2

0.24

0.42 BOD

TSS VSS

Ammonia

TKN

Phosphorus

Chloride Orthophosphate 2

4

6 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

BOD

TSS VSS

Ammonia

TKN

Phosphorus

Chloride Orthophosphate

PCA Analysis

-6 -4 -2 0 2 4

PC1

-4

-2

0

PC

2

0.6

Nitrate-N

Orthophosphate Overlay

-6 -4 -2 0 2 4

PC1

-4

-2

0

PC

2 Nitrate-N

Nek ton – B ag Seines

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.27

MR 1

MR 2

AR 1

AR 2

2D Stress: 0.05

MDS Configuration MDS Means Plot

MR 2

MR 3

AR 3

Nek ton – B ag Seines

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.27

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Season Spring

Summer

Fall

2D Stress: 0.27

MDS Configuration Seasonality

Nek ton - Trawls

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.16 MR 1 AR 1AR 2 AR 3

2D Stress: 0.01

MDS Configuration MDS Means Plot

MR 2

MR 3

Nek ton – Trawls

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.16

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Season Spring

Summer

Fall

2D Stress: 0.16

MDS Configuration Seasonality

Benthic Inverts (Middle)

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.13

MR 1

AR 1

2D Stress: 0

MDS Configuration MDS Means Plot

MR 2

MR 3

AR 2

AR 3

MD S : Dat a Overlays Transform: Fourt h root Transform: Fourt h roResemblance: S1 7 Bray Curti s similarity Resemblance: S1 7

2 D Stress: 0.27 Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

Bag Seines

ot

Bray Curtis similarity

Salinity

5

20

35

50

2D Stress: 0.27

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Nitrate-N

0.1

0.4

0.7

1

2D Stress: 0.27

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

D.O.

1

4

7

10

2D Stress: 0.27

MD S : Dat a Overlays Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Station MR 1

MR 2

MR 3

AR 1

AR 2

AR 3

2D Stress: 0.27

Bag Seines

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Bay anchovy

100

400

700

1E3

2D Stress: 0.27

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Western mosquitofish

6

24

42

60

2D Stress: 0.27

Transform: Fourth root

Resemblance: S17 Bray Curtis similarity

Atlantic croaker

4

16

28

40

2D Stress: 0.27

Taxonomic Diversity

20

40

60

80

100

De

lta +

A A

B

A B A B A B

20

40

60

80

100

De

lta +

ns

Bag

Seine

Collections

MR 1 MR 2 MR 3 AR 1 AR 2 AR 3

Station

0

2008 2009

Year

0

20

40

60

80

100

De

lta +

A

B

Spring Summer Fall

Season

0

Bag Seine Trawl Benthic Infauna Water Column Profile Water Chemistry

Bag Seine ___

Trawl 0.158* ___

Benthic Infauna

0.099 0.230* ___

Agreemen t Amon g th e MD S Configurations

Water Column Profile

0.125* 0.230* 0.033 ___

Water Chemistry

0.063 0.024 -0.033 0.471** ___

Sediments -0.002 0.051 -0.148 0.084 0.276*

Probability of obtaining a larger correlation coefficient by random chance

(based on 1,000 permutations) denoted by: * = prob. < 0.01, ** = prob. < 0.001.

Significant correlations (ρs > 0.3) identified in bold.

Routi ne Field Parameters

Routi ne Field Parameters

MDS Means Plot

W ater Chemistry

Highland Bayou

Diversion Canal

Mission River

Texas City Pump Canal

TCPC

HBDC

Mission River

W ater Chemistry

Highland Bayou

Diversion Canal

Mission River

Texas City Pump Canal

TCPC

HBDC

Mission River

Trawl Collections

Summer Season

Collections

Study Streams

Biological Physiochemical Sediment Instream Flow Habitat

Average Taxonomic Diversity �o

SIMPER analysis used to

identify the best “candidate

indicator taxa or

constituents”

Sensitivity of

indicator taxa to

changes in

“other”

chemical and

physical data

collected

Part C

Index Development

Biocriteria For

Tidal Streams Index Score

Coast-wide Nekton

Abundanc e Patter ns i n Seines

Bay anchovy Grass shrimp

Gulf menhaden Brown shrimp

White shrimp Silversides

Relationships to Env . Variables

*** Normalized Abundance

Trend line = Nonlinear form E[y] = a *

xb

Temperature Dissolved Oxygen Salinity

Coast-wide Nekton

Abundanc e Patterns i n Trawls

Bay anchovy Gulf menhaden

Blue catfish

Atlantic croaker

White shrimp

Silver seatrout

Relationships to Env . Variables

Temperature Dissolved Oxygen Salinity

Summary (M /A Study)

• Watershed characteristics (=Salinity) appears to be the dominan t factor structuring biologica l communities – Patter n consistent across th e multipl e levels of

ecologica l integrity measure d for a number of studiesstudies

– Eve n accountin g for extrem e drought conditions

• Lowermos t Stations had the mos t “distinct ” nekton communities – Gear dependent

– Agrees wit h th e Habitat assessment

Summary (M /A - Coastal)

• Benthic Communities (and Aquatic Inverts) responded less to Salinity – Dominate d by opportunistic taxa an d species

define d as “ubiquitous” – floodin g event re-sets

• • DDii ssssooll vveed d OO xxyyggeenn ll eessss ii mpmpoorrttaannt t tthh aann SS aallii nniitty y in structuring the biologica l communities – Many estuaries wit h seasona l hypoxi a ar e som e of

th e most producti ve systems for fisheries

• The extreme euryhaline / physiologica l abilities o f the nekton almos t precludes a spatially applicable “IBI”

Summary (M /A – Coastal)

• ________________________

Biological

Chemical Physical