gel electrophoresis l mathias. definition separation of dna fragments according to size, based on...
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Gel electrophoresis
L Mathias
Definition
• Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.
DNA negatively charged
DNA cut up using restriction endonucleases
Make up the gel which the DNA will be put into
Dye added to the DNA
Buffer solution added to the tank
DNA samples loaded into wells
Electrical current applied to the chamber
DNA is stained using ethidium bromide
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