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I Course Leica on Advanced Widefield and Confocal Microscopy and STED Super-resolution
From 7th - 9th July 2015
www.leica-microsystems.com
LECTURERSNathalie Garin, Leica Microsystems AG
Clément Laigle, Leica Microsystems SAS
Juan Luis Monteagudo, Leica Microsistemas S.L.
Javier Pazos, Leica Microsistemas S.L
Paco Porto, Leica Microsistemas S.L.
Paula Sampaio, Advanced Light Microscopy, Institute for Molecular and Cell Biology, Porto
INVITED SPEAKERSFábio Fernandes, Instituto Superior Técnico
José Rino Henriques, Bioimaging Unit, IMM
Renato Soccodato, Glial Cell Group, Institute for Molecular and Cell Biology, Porto
COLLABORATIONJosé Bessa, Vertebrate Development and Regeneration Group, IBMC
Luis Beine, Leica Microsistemas S.L.
Margarita Fité, Leica Microsistemas S.L.
Rita Matos, communications officer, IBMC
Augusta Monteiro, Molecular Genetics Group, IBMC
Mark Munro, Leica Microsistemas S.L.
Course coordinators:
Paula Sampaio (IBMC)
Juan Luis Monteagudo (Leica Microsistemas S.L.)
Leica Microsistemas, S.L. C/ Nicaragua, 4608029 BarcelonaTel. +34 93 494 95 55Fax +34 93 494 95 32
IBMCRua do Campo Alegre 8234150-180 Porto, Portugalwww.ibmc.up.pt
LOGISTIC INFORMATION
• From 7th - 9th July, 2015
• Venue: IBMC-Instituto de Biologia Molecular e Celular,
Rua do Campo Alegre 823, 4150-180 Porto, Portugal
• The practical sessions include working with multispectral STED
and light sheet (DLS - Digital Light Sheet) confocal systems
and FRAP microscopy for widefield applications.
• Registration fee: 350 Euros includes course material, lunches,
coffee breaks and course dinner.
• Those interested should apply by completing the pre-registration
form online at:
https://www.ibmc.up.pt/eventos/registrationnew.php?eid=76
For enquiries, contact Paula Sampaio
E-mail: sampaio@ibmc.up.pt
Tel.: 00351 226074900
• The number of places is limited to 15 people. Applicants will be
selected according background and motivation. After receipt of
acceptance e-mail, payment must be made prior to 26th June by
cheque or bank transfer.
SUGGESTED ACCOMMODATION• HF TUELA PORTO ***
Webpage: http://www.hfhotels.com/hf-tuela-po
• HOTEL DOURO *** Webpage: http://www.hoteldouro.pt
• YOUTH HOSTEL in PORTO Webpage: http://www.pousadasjuventude.pt
• SEMINÁRIO de VILAR Webpage: http://www.seminariodevilar.pt
GENERAL INFORMATION
Leica Microscopy course on Advanced Widefield & Confocal
Microscopy and STED Super-resolution.
Objectives:This course aims to provide extended technical skills in advanced
confocal imaging methods using state-of-the-art Leica equipment
including STED Super-resolution, light sheet microscopy (DLS), high
content analysis and widefield FRAP.
It combines introductory lectures and practical hands-on sessions.
During the practical sessions, students will be able to observe their
own samples.
Audience target:This course is aimed at all early career researchers, Post-Docs and
PhD students that wish to expand their theoretical and practical
knowledge in confocal microscopy and acquire skills in STED, high-
speed live cell imaging, DLS, molecular dynamics and data analysis.
PROGRAM
Tuesday 7th July 2015
09:00 - Registration09:15 - Welcome and course introduction. Paula Sampaio09:30 - Principles of fluorescence microscopy. Paula Sampaio10:30 - Coffee Break10:45 - Confocal Scanning Light Microscopy. Juan Monteagudo12:45 - Lunch14:00 - Practical Sessions I15:30 - Practical Sessions II17:00 - Coffee Break17:30 - Practical Sessions III19:00 - End of session
Wednesday 8th July 2015
09:30 - Live Cell Imaging. Paco Porto10:30 - Coffee Break10:45 - FRAP. José Rino Henriques11:45 - Super-resolution. Nathalie Garin12:45 - Lunch14:00 - Practical Sessions IV15:30 - Practical Sessions V17:00 - Coffee Break17:30 - Practical Sessions VI20:00 - Course Dinner
Thursday 9th July 2015
09:30 - FRET. Renato Soccodato10:30 - Coffee Break10:45 - FLIM & FCS. Fábio Fernandes11:45 - DLS. Clément Laigle14:00 - Practical Sessions VII15:30 - Practical Sessions VIII17:00 - Coffee Break17:30 - Practical Sessions IX19:00 - End of session
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