igv on cluster - tufts university · viewing the igv window if the genome browser is cut off by...
Post on 23-Apr-2020
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Start IGV on the Tufts HPC cluster• Using Chrome web browser, log in to ondemand.tufts.edu• Choose Interactive Apps->IGV• Choose desired time, >2 cores, 64 Gb memory• Click “Launch NoVNC” when it appears
Viewing the IGV windowIf the genome browser is cut off by the “Workspace” bar at the bottom -> Resize window using Chrome Zoom options
BAM Visualization with IGVChrom web browser to ondemand.tufts.edu -> login.Choose Interactive Apps->IGVClick “Launch NoVNC” when it appears
RNAseq PreferencesIf you are loading spliced RNAseq BAM files1. Go to View->Preferences
2. Click Alignments -> Track Display Preferences Options3. Check Splice Junction Track. 4. Click "OK"
Load Reference GenomeChoose reference genome by clicking “Genomes” on the top menu
• If you have mapped using a standard reference genome: Choose “Load Genome from Server” and highlight the genome you’d like to download. Leave “Download Sequence” unchecked
• If you mapped using a custom genome:Choose “Load Genome from File” and navigate to the genome location on the cluster. Note that the genome must be indexed e.g. with samtools prior to loading
Load BAMChoose BAM file by clicking on “File” on the top menu
• Navigate to the BAM location on the cluster.• Note that your BAM file must be sorted and
indexed e.g. by samtools
Type gene/contig name here (e.g. SUS1) or coordinates e.g. chrII:400,000-500,000
Zoom in and out
Select a region here to zoom
Splice junctions
BAM Visualization with IGV
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