introduction to the eukaryotic cell

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Introduction to the Eukaryotic Cell

Exercise 9

+Announcements

Students have time to observe Week 8 plates and finish their LNAs.

Post Lab 9/Pre Lab 10 are due by the time your lab meets next.

LNA Eukaryotic Cell is due next week in lab.

+Goals

To learn the basic structures associated with eukaryotic cells.

To identify the differences between animal and plant cells.

To understand the basis of cell culture, and the changes imposed on cells under culture conditions.

To understand the techniques of fluorescence and electron microscopy and cell culture.

+Animal Cell

+Plant Cell

+The Exercise Part I: Plant cells: Onion epidermis

Basic cell structure of a plant lacking chloroplasts Iodine: observe nucleolus Janus Green: observe mitochondria

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Part II: Animal cells: Human squamous epithelium Observe differences from the plant cells Do the mitochondria vary in size from those found in

plant cells?

The Exercise

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Part III: Autofluorescence View Tradescantia under the fluorescent microscope Chlorophyll pigments autofluoresce

The Exercise

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Part IV: Anacharis Leaf Cells View a living plant cell with chloroplasts

The Exercise

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Part V: Electron Micrographs and Cellular Infrastructure View EM transparencies Identify organelles You may want to take notes for future identification (i.e. Exam 2 and

Final)

The Exercise

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Be Able to Identify:Nucleus

Nuclear envelopeNuclear pore

NucleolusRough Endoplasmic Reticulum

RibosomeMitochondria

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Be Able to Identify:Golgi complex section viewtop view

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Golgi top view

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Golgi

section view

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Be Able to Identify:

Mitochondrion

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Be Able to Identify:

NucleusVacuole

Chloroplast

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Be Able to Identify:

Thylakoid

+ Summary of Exercise 9 Part I: Onion epidermis

slide x 3: water / Iodine / Janus Green size, nucleus, vacuole, cell wall/membrane, chloroplast,

mitochondria (?)

Part II: Cheek cells slide x 2: water / Janus Green size, nucleus, cell membrane, mitochondria (?)

Part III: Tradescantia TA Autofluorescence

Part IV: Anacharis Cytoplasmic streaming