invasive breast carcinoma cells from patients exhibit mena inv - and macrophage-dependent...

Invasive breast carcinoma cells from patients exhibit MenaINV- and macrophage-dependent transendothelial

migration

by Jeanine Pignatelli, Sumanta Goswami, Joan G. Jones, Thomas E. Rohan, Evan Pieri, Xiaoming Chen, Esther Adler, Dianne Cox, Sara Maleki, Anne Bresnick, Frank

B. Gertler, John S. Condeelis, and Maja H. Oktay

Sci. Signal.Volume 7(353):ra112-ra112

November 25, 2014

©2014 by American Association for the Advancement of Science

Correlation of Mena isoform expression to TMEM score.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

Correlation of MenaINV-TMEM scores in different grades of human IDCs.(A) Scatter plots of relative MenaINV transcript expression against TMEM score in IDCs of different grades.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

Correlation of E-cadherin and TMEM and MenaINV scores.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

iTEM assays using human patient IDC cells obtained from FNA biopsies.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

MenaINV is required for macrophage-induced iTEM in MDA-MB-231 TN breast cancer cells.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

CSF1R expression in iTEM-competent cells differs among breast cancer subtypes.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

Primary human macrophages increase iTEM of primary human breast cancer cells.

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science

Model of MenaINV- and macrophage-mediated tumor cell invasion and TEM.ITEM-competent human breast cancer cells from all clinically relevant subtypes display MenaINV high/Mena11a low

isoform expression and perivascular macrophages promote endothelia

Jeanine Pignatelli et al., Sci. Signal. 2014;7:ra112

©2014 by American Association for the Advancement of Science