laboratory of aquaculture & artemia reference center, ghent university, belgium artemia research...
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Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Artemia research at ARC
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
1. ARC ‘traditionals’: description of strain characteristics
• biometrics• hatching & diapause characteristics• lifespan & reproductive characteristics• effect of abiotic conditions on growth, reproduction….• cross-breeding• HUFA content• ……..
cooperation with other partners !
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
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2. Artemia population assessment studies
cfr. fluctuations in GreatSalt Lake harvests
• abiotic conditions• primary production• Artemia population
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Karabogaz-Gol, Turkmenistan
Russian Federation(Siberia)
China (Tibet)
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Orius
Podisus
3. Mass-rearing and biocontrol potential of predatory insects (Heteroptera, Coleoptera…) (Cooperation with Laboratory of Agrozoology, RUG)
Harmonia
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
nymph IV Orius
Cost-effective production of ‘killer bugs’ : production of large numbers at low price
Use of Lepidopteran eggs, esp. of Mediterranean flour moth, Ephestia kuhniella
• good development & reproduction of predatory insects
• high price (high investment & production costs, high demand, occasional market shortages)
• possible human health problems (allergene)
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Promising preliminary results : Artemia cysts as alternative to Ephestia eggs
effect on growth, metamorphosis, survival, reproduction of predator…
application techniques (decapsulated vs. non-decapsulated, hydration level…)
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
4. Proposal for a new VIB (Flemish Institute of Biotechnology) Department : Department of Microbial Ecology of Aquatic Invertebrates
Ghent University, Belgium• Laboratory of Microbial Ecology and Technology• Laboratory of Aquaculture & Artemia Reference Center
University of Connecticut, USADalhousie University, Canada
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Rationale :Eukaryotes exist in close association with micro-organisms: symbiotic, pathogenic, neutral= EMCs (eukaryote microbial communities) Colonisation of EMCs by allochthonous organisms = 1st step in development of pathogenesis, commensalism, symbiosis.
Objective : How do allochthonous micro-organisms establish themselves in the EMCs ? What factors determine success or failure ?
new insights into host-pathogen and host-probiotic interactions therapeutic and probiotic opportunities.
Model eukaryote: Artemia franciscana Model allochthonous organisms: Vibrionaceae
• pathogenic or beneficial to Artemia• culture collection available; extensive database available of chromosomal & mobile genetic elements important to Vibrio success in EMCs • common pathogens of almost all higher eukaryotes,
develop a comprehensive model of Vibrionaceae invasion and colonisation
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Host-Microbe Unit
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Microbe-Microbe Unit
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Mobile Genetic Element Unit
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
5. FWO project: the functional role and characteristics of micro-organisms in the larviculture of aquatic organisms with Artemia as a test species
Rationale :
• general trend towards more natural suppression of opportunistic/obligate pathogens in aquaculture• consensus about critical role of microbial interactions
• numerous questions about biological processes involved :competition for adhesion sites in digestive tractcompetition for nutrientsexcretion of antibacterial products by bacteria/micro-algaestimulation of immune system by algae/yeasts/bacteria………
• test organisms not cultured axenically interactions with micro-organisms
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
opportunistic bacteria
pathogenic bacteria
probiotic bacteria
Artemia as model organism
partners: • Laboratory of Aquaculture & Artemia Reference Center (RUG)• Prof. Godelieve Criel (RUG)• Laboratory for Microbial Ecology (RUG)• Laboratory for Aquatic Ecology & Aquaculture (KULeuven)
Objective: Study the mode of action of microbial organisms & verify hypotheses byuse of axenically cultured organism
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
research questions: 1. How do bacteria colonize the gut??
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
ingestion?
through the cuticula?
through the gills?
research questions: 1. How do bacteria colonize the gut?2. How does a pathogen infect an aquatic organism?
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
research questions: 1. How do bacteria colonize the gut?2. How does a pathogen infect an aquatic organism?3. How does a ‘probiotic’ protect an aquatic organism?
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
First action: develop an axenic standard culture of Artemia
autoclaved
decapsulated
-irradiated
culture vessels
food
cysts
seawater
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
Further actions:
Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Belgium
• Linked to the axenic cultures and using scanning-electron microscopy, an ‘atlas’ of a ‘normal’ gut of successive developmental stages would be compiled.
• At a later stage, the colonization of the gut by selected harmless and pathogenic bacteria will be studied quantitatively and qualitatively using diverse microbial marker techniques (FiSH, DGE, GFP).
• Besides, we will consider axenic Dunaliella cultures as food source in order to investigate the possible probiotic role of algae.
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