msu amy iezzoni (pd) jim hancock dechun wang cholani weebadde wsu cameron peace dorrie main
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MSUAmy Iezzoni (PD)
Jim HancockDechun Wang
Cholani Weebadde
WSUCameron Peace
Dorrie MainKate Evans
Karina Gallardo Vicki McCracken
Nnadozie OraguzieFormer WSU
Raymond JussaumeMykel Taylor
CornellSusan Brown
Kenong Xu
Clemson Ksenija Gasic
Gregory Reighard
Texas A&MDave Byrne
Univ. of CA-DavisTom Gradziel
Carlos Crisosto
Univ. of New Hamp.Tom Davis
Univ. of MinnesotaJim Luby
Chengyan Yue
Oregon State Univ.Alexandra Stone
Plant Research Intl, Netherlands
Eric van de WegMarco Bink
USDA-ARSNahla Bassil
Gennaro FazioChad Finn
Univ. of ArkansasJohn Clark
Acknowledgements
This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture
Plant Research International, NetherlandsEast Malling Research, UKCRA-FRU, Rome, ItalyIASMA, Trento, ItalyINRA (Bordeaux, Avignon, Angers), France
Andres Bello University, Chile Plant & Food Research, New Zealand
ARC, South Africa
RosBREED participant
location
International Collaborators
+ 2 Scientific Advisory Panel members (Spain, New Zealand)
Discovering a QTL A major milestone or a stepping-stone into
practical marker-assisted breeding?
Cholani Weebadde
25 July 2013ASHS, Palm Desert, CA
Outline of Presentation
RosBREED
How does QTL discovery inform MAB?
The RosBREED MAB pipeline - a strategy to put DNA knowledge into practical application
Workshop presentations
How does QTL discovery inform Marker-Assisted Breeding
(MAB)?
WA2
Which wild germplasm to use?
Which traits to target? Which parents & combinations to create?
Which seedlings to progress?
Which selections to trial?
Which advanced selections to commercialize?
What is Marker-Assisted Breeding? Use of DNA information to support breeding decisions
What published QTLs look like
A bump on the chromosome
What does it tell the breeder??? Nothing much really! Just that there may be a gene in the “bump region” of the chromosome that is associated with the trait
Known QTLs and their application
Hundreds of published QTLs are currently available for Rosaceae crops
Of these, only a few were being used for MAB
RosBREED has bridged this chasm
Genomics Resources
Marker- assisted breeding
100s of QTLs
published
More efficient development of new
cultivars
RosBREED is dedicated to putting DNA knowledge into practical application to increase the efficiency of rosaceous crop breeding
RosBREED
RosBREED Mission Statement
We will develop and apply marker-assisted breeding, based on improved knowledge of industry value and consumer preferences, to accelerate and increase
the efficiency of rosaceous cultivar release and successful cultivar adoption
4 yrs$7.2M federal$7.2M matching
Amy Iezzoni, Michigan State Univ.Cameron Peace, Wash. State Univ.
This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture
Demonstration Breeders
&
Breeding Trainees
The RosBREED MAB Pipeline: a strategy to put DNA knowledge into practical
application….
How we bridged the Chasm….
Find
An individual’s genome is the full complement of genetic information that it inherited from its parents.
Within this vast repertoire of genetic information, individual’s genes are being discovered that control critical production and fruit quality traits.
Put into application in breeding
Jewels= valuable genetic discoveries ready for breeding application= marker-locus-trait associations validated in a breeding program
Polish
Jewels in the genome analogy
Lots of Shiny Rocks! So What?!!
Each breeder needs to appraise for themselves all these “gemstones”…
…to decide which onesare worth polishing…
www.
rock
tum
bler
.com
…to find the “jewels”!
RosBREEDAroma
PhytonutrientShape
TartnessJuiciness
SizeOther fruit quality attributes
Storage lifeDisease resistance
No storage disordersExternal appearance
Sugar/acid balanceSweetness/soluble solids
Shelf life at retailFruit firmnessExterior colorFruit crispness
Fruit flavor
0 20 40 60 80 100
2
2
27
9676
1543
33
5
13
335
2610
23
54
777
7
2341
Socio-Economics Surveys (example for apple)
Breeders Market Intermediaries
Michigan Producers
Washington Producers
% of respondents
MOST
important
traits
LEAST
important
traits
So that resources for development of genetic tests (polishing the shiny rocks) can be directed to traits with the highest importance to stakeholders….
• Integrate DNA information with conventional breeding, systematically (8-stage MAB Pipeline)
RosBREED MAB Pipeline
“Jewels in the Genome” Available Now!
Information on the jewels on eXtension
“RosBREED Jewels are now featured in eXtension”
Workshop presentations
Known QTLs
Application in breeding
Breeding markersDNA te
sting
serv
icesQTL
prior
ities
Cross
ing
sche
mes
Trial
MASS
Simple validation
Allele effects& distributions
Seedli
ng
selec
tion
sche
mes
RosBREED success stories: QTL and QTL allele validation in Cherry, Apple and Peach
1. Amy Iezzoni – Cherry
2. Sujeet Verma – Apple
3. Ksenija Gasic - Peach
Known QTLs
Application in breeding
Breeding markersDNA te
sting
serv
icesQTL
prior
ities
Cross
ing
sche
mes
Trial
MASS
Simple validation
Allele effects& distributions
Seedli
ng
selec
tion
sche
mes
Marker-Assisted Breeding for Apple: Experiences from University of Minnesota and
Washington State University
Jim LubyRosBREED Breeding Team Lead
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