nrdr 2.0 tutorial - the non-coding rna databases resource tutorial

Tutorial:

Searching on NRDR 2.0

This tutorial shows you how to perform the following 4 searches in NRDR 2.0:

•  Searching siRNAs involved in Cancer

•  Searching human miRNAs in databases with similarity search tool

•  Searching databases containing FASTA files for download

•  Downloading all sequences available for Arabidopsis thaliana

Example 1 Searching siRNAs involved in Cancer

* Speficy cancer as a word in Overview

* Select siRNA as ncRNA class

Example 1 Searching siRNAs involved in Cancer

* Click here to continue

Example 1 Searching siRNAs involved in Cancer

Example 1 Searching siRNAs involved in Cancer

You should see something like this:

Example 1 Searching siRNAs involved in Cancer

* Click here to see more information about this

You should see something like this:

Example 2 Searching human miRNAs in databases with similarity

search tool

* Specify Homo sapiens as organism

Example 2 Searching human miRNAs in databases with similarity

search tool

* Select miRNA as ncRNA class

Example 2 Searching human miRNAs in databases with similarity

search tool

* Specify Similarity as search method

Example 2 Searching human miRNAs in databases with similarity

search tool

* Click here to continue

Example 2 Searching human miRNAs in databases with similarity

search tool

You should see something like this:

Example 3 Searching databases containing FASTA files for

download

* Only specify FASTA in download type

* Click here to continue

Example 3 Searching databases containing FASTA files for

download

You should see something like this:

Example 3 Searching databases containing FASTA files for

download

Example 4 Downloading all sequences available for Arabidopsis

thaliana

* Click on Download at the Main menu

Example 4 Downloading all sequences available for Arabidopsis

thaliana

* Specify Arabidopsis thaliana as your organism

Thank You!

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