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Physiology lab #1… RBC counting

Hemocytometer:

It’s a kind of glass slide, we’ll use it in this lab as well as the coming one.

the area of RBC counting in which we’re concerned has the following

dimensions and divisions :

You counted the RBC’s in a volume of 0.004 mm^3, remember that you

diluted the sample now for the calculations ( assuming that you counted

120 RBC\0.004 mm^3 ):

o 120 * 25 = 3000/0.1mm^3 … (i.e. in the whole RBC counting area)

o 3000 * 10 = 30000/mm^3 … diluted

o 30000*200(the dilution factor)= 6,000,000/mm^3 … not diluted

Those are notes I’ve written in the lab

..they’re particularly useful for those

who did not attend it, they include

extra things about the methods only

Area for RBC counting

Physiology lab #2… WBC counting

Each of the 4 squares used in WBC counting has a volume of:

1 mm * 1mm * 0.1 mm= 0.1 mm^3

Count WBC’s in each then take the avg of them

For dilution : 1ml blood in 19 ml saline dilution factor = 20

You counted the WBC’s in a volume of 0.1 mm^3 , remember that you diluted

the sample now for the calculations ( assuming that you counted

40WBC\0.1 mm^3 ):

o 40 * 10 = 400/mm^3 … diluted

o 400*20(the dilution factor)= 8000/mm^3 … not diluted

Areas forWBC counting(bold borders)

Depth = 0.1 mm

1 mm

1 mm

Physiology lab #3

I. Hb concentration

o Take 20 microliter of blood .

o Add 2 -3 drops of HCL

o You’ll get “ferrohematinic acid” , dark brown color

o Dilute it with distilled water till you get the same color as the standard

solution

o Then check the grade on the tube

II. HcT…. H1/H2 * 100%

Centrifuged blood sample

Heig

ht 1

Heig

ht 2