practical microbiology

Practical Microbiology

Ibrahim Mahmoud Sayed Ibrahim

Instrument

Instruments

1-Seitz filter

2—MacKnotoch 3-Gas Pack system

2 -IMVC test

•IMVC of E.coli •

IMVC test

Citrobacter Klebsellia

TSI (triple sugar iron)•LF (Ecoli) Pseudomonas Proteus

Christen urea media Simmon Citrate

•Pink +ve proteus Green –ve Ecoli

•Yellow –ve Blue +ve Klebellia

Sugar fermentation of Salmonella

•S.paratyphi B

•S.paratyphi A S.typhi

Sugar fermentation of Shigella

•Sh.dysentriae Sh.flexenerii Sh.Sonneii

Sugar Fermentation of Neisseria

•N.gonorrhea N.meningitidis

Non-inoculated Media

•Loffler’s serum Dorset Egg Lowstein Jensen media

Non inoculated media

TCBS Anaerobic media Thioglycolate –Robertson cooked meat-Deep agar

II inoculated mediaNutrient Agar

Staph (endopigment) Pseudomonas (Exopigment)

Proteus (swarming) Sabroud dextrose (yeast)

II-Blood agar•Blood agar (B-hemolysis) as Staph aureus, Strept Pyogens

•Blood tellurite as Corynebacterium diphtheriae

III Chocholate agar•Chocholate agar Optochin sensitivity

• Pneumococci +ve zone : Pneumococci•- ve zone : S.viridans

IV) Pink Plate •Mannitol salt agar MacConkey Agar

• Staph aureus Lf (Ecoli)

V) EMB plate

•E.coli Klebsellia

VI) Special Plate•MacConkey Agar Sabroud dextrose Agar

•NLF(Salmonella) Mould

VI) Special Plate

•Milk Agar Bismuth Sulphite•Staph Salmonella