presented by: dr. shivi maini clinical research- r&d, ayurvet limited
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METHIONINE SUPPLEMENTATION OPTIONS IN POULTRY
Presented by:Dr. Shivi Maini
Clinical Research- R&D,Ayurvet Limited
Introduction
Scientific Validations and Recommendations
Overview of Synthetic DL Methionine Supplementation in Poultry
Methionine Supplementation Options
Key Features, Benefits and Advantages of Product
Methionine sulphoxide
Methionine sulphoxide
Methionine in natural feedstuff (present in L form) is limited
Low Biological Availability
DL Methionine in basal ration is added to meet
the requirement
Replaceable
Non-Replacea
ble
Storming issues with synthetic amino acid: SCIENTIFIC REPORTS
• Possible synthetic Hazards• Residues in Synthetic Feed Supplements• Unjustified in organic productions• Global Food Safety & Security Concerns
Methionine Toxicity
Sulphate Release
Poor Bone Mineralization
Oxidation of Excess
Methionine
Immunosuppression
Thinning of Egg Shells
Metabolic Acidosis
Low Body Wt Gain
Poor egg and Shell Quality
EXCESS OF SYNTHETIC METHIONINE IN POULTRY FEED MAY HAMPER GROWTH & PERFORMANCE LEADING TO REVERSE EFFECTS.. Diseases of Poultry page No. 1028, Iowa state press
Excess Methionine
FLUCTUATIONS IN DL Methionine PRICE
Sept, 14 Oct,14 Nov,14 Dec,14 Jan, 15 Feb, 15 March,15 April, 15 May,15 June,150
100
200
300
400
500
600
310
535
508
500
495495
480
500
520
535
Price (Rs.) of DLM (99%)
HERBAL FEED ADDITIVES: A SAFE, SCIENTIFIC & SUSTAINABLE
APPROACH
PHYTOADDITIVE REPLACEMENT OPTIONS
• Herbs are known and documented for having highly precursors of vital AA
• Mimics the activity of Methionine etc
Sweeter
Safer
Same taste
Cicer arientinum
Phaseolus mungo
Triticum sativum Mucuna pruriens
Allium cepa
MAJOR CONSTITUENT HERBS WITH BIOMIMICKING ACTION
NATURAL ALTERNATIVE
Contains Standardized Herbal Extract – Mimics the activity of Methionine
Rich in precursors of Methionine/SAMe
Fits strategically in Methionine Cycle in body to provide optimum Function
Non- toxic No residual effects Environmental Friendly Safe to Livestock Safe to user
Novel feed additive
For partial replacement of synthetic methionine
BIOMIMICKING ACITIVITY OF HERBS
DL Methionine
L Dopa Mucuna pruriens
Better MAT Activity
Increases SAMe Formation
.Zhao WQ, Latinwo L, Liu XX, Lee ES, Lamango N, Charlton CG. L-dopa upregulates the expression and activities of methionine adenosyl transferase and catechol-O-methyltransferase. Exp Neurol. 2001 Sep;171(1):127-38.
Methionine ATP
S-Adenosyl Methionine (SAMe)
Transmethylation reaction
Methyl Group
Homocysteine
SAH (S-Adenosyl Homocysteine)
(Allicin) Allium sativa
mimics the activities of cysteine prodrugs such as N-acetyl cysteine (NAC) and S-adenosyl methionine (SAM)
Ref: Bernas GC, Gonzales RE, Solevilla RC, Ysrael MC. Pharmacology-Toxicology. In: Guevara BQ, editor. A guide book to plant screening: Phytochemical and biological. Santo Tomas Philippines: Research Centre for Natural Sciences, university of Santo Tomas; 2004. P. 103-132.
Corcoran GB, Wong BK. Role of glutathione in prevention of acetaminophen-induced hepatotoxicity by N-acetyl-L-cysteine in vivo: studies with N-acetyl-D-cysteine in mice. J Pharmacol Exp Ther 1986;238(1):54-61.
Cicer arientinum
Cicer arientinum enhances the bioavailability of partial or limiting levels of DL Methionine
Reference: Arch Latinoam Nutr. 1975 Dec;25(4):363-75. [Limiting amino acids of chickpeas (Cicer arietinum) for chickens and pigs]. [Article in Spanish] Shimada A, González EA.
Scientific Validation Studies on METHIOREP
16 National Research Trials
4 International Research Trials
4 Thesis (2 MVSc+ 2 PhD)
12 Scientific Publications
SCIENTIFIC VALIDATIONS ON METHIOREP :
BROILER, OUAT Bhubaneswar, Orissa
LAYER, IPPM, TANUVAS, Chennai
PIG GROWERS, PGRIAS, Kattupakam, TN
BROILER, Field trial in 10 farms, Bidar , Karnataka
Layer, 0-40 wk study, PGIVAS, Akola, MS
16
MQA Broiler Study, Udgir, MS
BROILER, Field trial in 10 farms, Bidar , Karnataka
BROILER, Project Directorate of Poultry
BROILER, College of Vet. Sci. Hyderabad: (3 trials)
broiler, Durg Vety College, ChattisgarhLayer Durg Vety College, Chattisgarh
broiler, College of Vet. Sci. , Uttar Pradesh
Cattle, Nagpur vety college , MS
Cattle, Nagpur vety college , MS
Trial conducted by:Dr Borja Vila
RECERCA I TECHNOLOGIA AGROALIMENTARIESMonogastric Nutrition
Department of Animal Nutrition, Centre de Mas BoveI,
Reus, Barcelona, SPAIN.
SCIENTIFIC VALIDATION WITH INTERNATIONAL COLLABORATORS
STUDY INVESTIGATOR: Woo Kyun Kim, Ph.D.Assistant Professor
303 Poultry Science BuildingDepartment of Poultry Science
University of GeorgiaAthens, Georgia 30602
STUDY INVESTIGATOR: Dr. José A. Garcia Regueiro (Ph. D.)
IRTA MonellsFunctionality and Nutritionjoseantonio.garcia@irta.cat
www.irta.cat
Trial conducted by:Dr Borja Vila
RECERCA I TECHNOLOGIA AGROALIMENTARIES
Monogastric NutritionDepartment of Animal
Nutrition, Centre de Mas BoveI,
Reus, Barcelona, SPAIN.
Study 1: Comparative efficacy evaluation of Methiorep & synthetic Methionine in improving
growth at partial replacement
EXPERIEMENTAL DESIGN
660 day old male Ross 308 broiler chicken were procured from commercial hatchery. (5 replicates of 33 chick each/group)
Experiemental duration: 5 weeks
GROUP I - No added synthetic/herbal methionine GROUP II - Synthetic methionine @0.2% (2Kg/tonne
of feed)GROUP III - 1/3 replacement of synthetic
methionine by MethiorepGROUP IV - 1/2 replacement of synthetic
methionine by Methiorep
MEAN BODY WEIGHT (Kg) AT 5th WEEK
Group I Group II Group III Group IV2.2
2.252.3
2.352.4
2.452.5
2.552.6
2.652.7
2.36
2.642.66 2.65
Average live weight at the end of the experiment
Significantly higher body weight was recorded in combination groups III & IV & DLM supplemented group II as compared to
untreated control group I
Average Daily Feed Intake Per Bird
Group I Group II Group III Group IV92949698
100102104106108
97.6
106.5 106103.3
Average daily feed intake (g)
Average daily feed intake non significantly different in treatment groups
FEED EFFICIENCY
Group I Group II Group III
Group IV1.441.461.48
1.51.521.541.561.58
1.61.62
1.607
1.508
1.5089999999999
9
1.5169999999999
9
Non-significa
ntly different among treatme
nts
Dressing Percentage (%)
Group I Group II Group III
Group IV
8181.5
8282.5
83
81.6
82.882.4 82.4
Series1
A non-significant difference in Dressing % was recorded among treated groups II, III & IV, however it was
significantly better than untreated group I
Organoleptic and Sensory Parameters
Sensory Evaluation of Microwave cooked meatIt was done using eight point Scale
Appearance
6.3 6.66
Control
DLM + Methiorep (1:1)
Economics of ProductionPARTICULARS GROUPS
IIIII (DLM + Methiorep)1/3 replacement
IV (DLM + Methiorep)1/2 replacement
Cost of ChickRs. 19/-
Rs. 19/- Rs. 19/-
Avg. Feed consumed/chick (0-42days) 3.73 kg
3.71 kg 3.62 kg
Cost of feed@Rs.25/kgRs. 93.25/-
Rs.92.75/- Rs.90.50/-
Cost of additives DL Methionine@ Rs525/-Methiorep@Rs.135
Rs.3.91 /-R s. 0.324/- + Rs. 2.57
= Rs. 2.89R s 0.48/- + Rs 1.90
= Rs. 2.38
Misc. costRs. 15/-
Rs. 15/- Rs. 15/-
Wt of broiler2.64kg
2.66kg 2.65 kg
Cost of broiler@Rs. 75/kgRs.198/- Rs.199.50 Rs.198.75/-
Profit : Cost of Broiler- (cost of chick+misc. + feed + additive) [198 - (19+93.25+3.91+15)=
Rs. 66.84/- chick
[199.50 - (19+92.75+2.89+15) =
Rs. 69.86/bird
[198.75 - (19+90.50+2.38+15)=
Rs.71.87
Loss due to mortality (per bird)1.5/-
0.74/- 0.72/-
Net profitRs. 65.34/bird
Rs. 69.12/- Rs. 71.15/-
NET PROFIT OVER CONTROL GROUP --- Rs. 3.78/- Rs.5.81/-
CONCLUSION
Partially replacing DL –methionine by Methiorep by 1/3 or ½ was well comparable to DLM supplementation alone in terms of growth performance & carcass yield.
Improving the overall economics of production as Methiorep costs less than DL-Methionine.
Recommended use of Methiorep for multiplying your Profits.
INTERNATIONAL POULTRY SCIENTIFIC FORUMATLANTA, USA, JANUARY 27–28, 2014
Publication
TRIAL STATUS
TITLE OF PROJECT:
Comparative efficacy evaluation of Methiorep and DLM supplementation in broilers
INVESTIGATOR
Dr. Park W. Waldroup, Poultry Science Department, University of Arkansas, Fayetteville AR 72701 PWW- 2010-08
TRIAL RESULT IN BRIEF:
Study 2. International trial on Methiorep : USA
Results of these studies shows that herbal methionine product (Methiorep) can be used for partial replacement of Synthetic methionine in broiler diet. It appears to work in combination with dl methionine (67/33) at 0.3% level of inclusion in supporting body weight
gain of the broiler chick; which is cost effective on basis CB Analysis..
Study3: International trial Methiorep
STUDY INVESTIGATOR:
Woo Kyun Kim, Ph.D.Assistant Professor
303 Poultry Science BuildingDepartment of Poultry Science
University of GeorgiaAthens, Georgia 30602
INTESTINAL AMINO ACID TRANSPORTERS:
°AT-1 (SLC family 6 member 19) and LAT-1 (SLC family 7 member 7)
LIVER NUTRIENT METABOLISMS USING REAL-TIME PCR:
IFN-gamma Glucose metabolism (PEPCK) Pyruvate Kinase (PK)Glucose Transporter 2 (GLUT2) Pyruvate Dehyrogenase (PDH)) Lipid metabolism (Acetyl-CoA acetyltransferase 2 (ACAT2) Peroxisome Proliferator Receptor-gamma (PPAR-gamma)Lipoprotein Lipase (LPL), Fatty acid synthase (FAS)
OBJECTIVE : To evaluate the effects of herbal Methiorep on pullet growth, feed efficiency, immunity, amino acid transporters, and liver nutrient metabolisms in pullets.
To evaluate the effects of herbal Methiorep on feed efficiency, egg production and egg quality in laying hens
PARAMETERS STUDIED: Growth, Performance & Production Feed consumption Body weight Feed efficiency Mortality uptil 18 weeks Egg production Egg quality parameters
GENE EXPRESSION STUDIES RELATED TO IMMUNE SYSTEMS: IL-10 TLR-4 IL-1 IL-6
2: International trial Methiorep
Experiemental Design
Study I (0-40 wk): This study will be conducted to evaluate the effects of herbal
Methiorep on pullet growth, feed efficiency, immunity, amino acid transporters
and liver nutrient metabolisms in pullets.
A total of 208 1-day old Leghorn pullets (4 trt. x 6 rep x 13 birds per cage) will be
randomly allocated to 4 treatment groups:
1) Negative control (No synthetic DL-Met supplemented)
2) Positive control ( 0.2% DL-Meth supplemented)
3) DL-Met (50%) + herbal Methiorep (50%)
4) DL-Met (67%) + herbal Methiorep (33%)
Summary of Results: S.NO.
PARAMETERS (AVG.) CONTROL GROUP
(WITHOUT DLM)
DLM (0.2%) METHIOREP (0.1%)
+ DLM (0.1%)
METHIOREP (0.133%)+ DLM
(0.067%)
1 FEED EFFICIENCY (19-40 WK)
2.26 1.92 1.94 1.95
2 AVG. EGG PRODUCTION % 91.08 96.24 97.42 96.67
3 MEAN EGG WEIGHT (G) 52.75 56.40 56.06 56.07
4 MEAN SPECIFIC GRAVITY 1.085 1.087 1.088 1.088
5 Mean Haugh Unit (22-40 weeks)
98.62 97.98 98.40 99.85
6 YOLK COLOUR BRIGHTNESS 58.36 58.41 58.36 58.01
7 YOLK COLOUR REDNESS 3.84 3.94 3.91 3.93
8 SHELL WEIGHT (G) 5.27 5.54 5.42 5.49
Feedback on Product usage experience by Investigator
Study 4: International trial Methiorep
STUDY INVESTIGATOR:
Dr. José A. Garcia Regueiro (Ph. D.)IRTA Monells
Functionality and Nutritionjoseantonio.garcia@irta.cat
www.irta.cat
OXIDATIVE STATUS
TBARS Antioxidant enzymatic profile
OTHER METABOLITES
Carnitine Taurine 5-methyl- THF Vitamin B12 VITAMIN B6 Adrenaline Total fatty acids Glucose Total Protein
OBJECTIVE : The effect of two products on specific biomarkers and related metabolites in blood from a poultry animal model.
PARAMETERS TESTED:
Metabolite Assay HomocysteineCysteineGlu-CysCys-Gly GSHGSSG.
Amino acid profile in plasma: SAMe Using HPLC/ DAD & MS/MS
Identification of chemical markers of herbal substances
International Trial: Methiorep
VALIDATIONS SUMMARY
• The most important result was a positive correlation between Methionine/ Methiorep
Cysteine and Homo-Cysteine with B12, this is an expected result but could be useful as a way
to validate the effect of the treatments.
• Rutin & L DOPA was isolated from Methiorep supplemented group.
• Ratios Cys/Met and Ser/Met:
Ratio Cys/Met :was lower for DL-Methionine (2.03 at 360 h) and that for of DL-
Methionine+Methiorep (2.26 at 360 h).
Ser/Met ratio showed a similar pattern with the lowest value for DL-Methionine (3.32 at 360
h) and that for DL-Methionine+Methiorep showed a value of 3.74 at 360 h.
• These results evidenced a strong effect of DL-Methionine in the concentration of Methionine
that requires a lowest quantity of Ser to produce Methionine.
STUDY 5: STUDIES ON COMPARATIVE EFFICACY OF HERBAL AND
SYNTHETIC METHIONINE ON PERFORMANCE OF VEN COB BROILER
PhD THESIS (2011) Submitted at COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY, ANJORA, DURG (C.G.) 491001, INDIRA GANDHI KRISHI VISHWAVIDYALAYA, RAIPUR (C.G.)
Albumin mg/dl Serum Globulin g/dl Serum total protein g/dl
SGPT U/L0123456789
Basal Diet
Herbal methionine
DL Methionine
Effect of herbal & synthetic methionine on Blood biochemical constituents
Hb g/dl PCV % TLC Heterophil Lymphocytes0
10
20
30
40
50
60
9
29.5
19.56
38.25
50.75
9.6528.75
18.27
37
50.75
9.81
28.33
18.85
37
52.75
Basal Diet
Herbal methionine
DL Methionine
DLM 2
DLM + HM
DLM 1
Effect of Methionine supplementation on Haematological parameters
CONCLUSION
The growth performance, feed efficiency &
biochemical parameters in DLM & Methiorep
supplemented group II are well comparable to DLM supplementation alone
(group I)
STUDY 6: COMPARATIVE EFFICACY OF SUPPLEMENTATION OF SYNTHETIC AMINO ACIDS
WITH HERBAL ALTERNATIVES IN LAYERS
PhD Thesis :
submitted to Deptt. of Animal Nutrition,
College of Veterinary And Animal Sciences,
Durg, Chhattisgarh, India. 2012
Methionine supplemented groups were having higher egg weight
Basal diet Synthetic Methionine Herbal Methionine47
48
49
50
51
52
53
54
55
56
57
50.45
56.2556.13
Egg weight (g)
Higher egg mass was recorded with methionine supplementation
Basal
die
t
Synt
hetic
Met
hion
ine
Her
bal M
ethi
onin
e46
48
50
52 48.05
51.8 51.78Egg mass (g egg/ hen per day)
Avg. no .of eggs/ hen from 18-35 weeks
Group 1 (without DLM)
Group II (DLM) Group III (DLM + Methiorep)
84
86
88
90
92
94
96
98
100
102
90.55
101.15 100.84
Avg. No. of Eggs/Bird
A significant increase in number of eggs
produced per hen was evident in DL
Methionine group II and DLM +Methiorep
supplemented group III during experiemental
period.
Yolk Albumen Shell0
10
20
30
40
50
60
70
25.76
64.88
9.03
26.09
64.89
9.0227
64.9
9.02
Basal dietSynthetic MethionineHerbal Methionine
EGG COMPONENT PERCENTAGE
Basal diet Synthetic Methionine Herbal Methionine0
102030405060708090
42.28
78.37 80.7
Economics/Net Profit/bird (Rs.)
Herbal Methionine supplementation was found to be most economical….!!!
METHIOREPphytoadditive for replacement of synthetic methionine
BENEFITS
Natural analogue to replace synthetic methionine to cut your methionine supplementation
cost.
Economic & can give better performance of birds.
Powder form ensures equivalent distribution of the product in the feed.
Its activity remain unaltered during pelleting.
RECOMMENDED USAGE
BROILERS (1:1)
Methiorep: 1 part Methiorep and 1 part DL Methionine from day 1 to marketing age.
LAYERS (1:2)
Methiorep: 1 part Methiorep and 2 part DL Methionine from 1st week to 72nd week.
BREEDERS (1:3)
Methiorep: 1 part Methiorep and 3 part DL Methionine from 1st week to 72nd week.
I’d like another sack of the “METHIOREP” you sold me last week!!
This is an Ayurvet R&D presentation
Thank You!Questions invited!!
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