reprogramming of differentiated cells into stem cells or other somatic cells

Reprogramming of differentiated cells into stem cells or other

somatic cells

Content1. Basics 2. Stem cells3. Transcription factors4. Reprogramming of differentiated cells5. Method of reprogramming into a pluripotent stem cells6. Reprogramming of cells into multipotent stem cells7. Direct reprogramming into another somatic cell8. Alternative methods of reprogramming9. Vector systems 10. Possible areas of use for this methods

Basics

• Stem cells

• Transcriptionfactors

Aktivator

Bindung an Enhancer

Verstärkung der Transkription

Bindung an Operator

Stabilisation der RNS-

Polymerase

Bindung an Coaktivator

Verstärkung der Transkription

Katalysation der Acetylierung

Offenere DNS = Hochregulation

Repressor

Bindung an Operator

Verhindern der Transkription

Bindung an Corepressor

Abregulierung der Transkription

Katalysieren der Deacetylierung

Engere DNS = Verminderung

der Transkription

Bindung an einen Silencer

Herabregulation der Transkription

Reprogramming of differentiated cells

• Method of reprogramming into a pluripotent stem cells

• Reprogramming of cells into multipotent stem cells

Transkriptionsfaktoren

Somatische Zelle

Multipotente Zelle

Zellen eines bestimmten Keimblattes

• Direct reprogramming into another somatic cell

Transkriptionsfaktoren

Somatische Zelle

Somatische Zelle des eigenen Keimblattes

Somatische Zelle eines anderen Keimblattes

• Alternative methods of reprogramming

TranskriptionsfaktorenTranskriptionsfaktoren +

Chemische Verbindungen

mikro-RNS

Somatische Zellen

Pluripotente Stammzelle

Zellen der drei Keimblätter

Zellen der Keimbahn

• Vector systems

Retrovirus

Lentivirus

• Possible areas of use for this methods

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