smbe satellite meeting on eukaryotic -omics

#SMBEeuks

April 29 – May 2, 2013UC Davis Conference Center

Logistics

• Wi-Fi• Lunch/Dinner

Logistics

• Wi-Fi• Lunch/Dinner• Instructions for posters• Instructions for talks• Sponsor thanks• Prize for travel award recipients

It all started in Florida…

2011 Catalysis Meeting at NESCent

Future Goal:

ResearchCoordinationNetwork

Building a Eukaryotic Community

• Relaxed meeting schedule• Emphasis on speaker diversity• Breakout groups on Thursday - outputs

• Get to know your neighbor – socialize!• Tweet! (Profusely, if possible)

Photo Credit: J. Baldwin, M. Mundo-Ocampo

Biodiversity?

Phylogeography?

Environmental Impacts?

Extract Environmental DNA

Amplify rRNA

High-throughput sequencing

Community analysis

Diverse marine community

EASYEASY

EASY

VERY Difficult!

http://phylosift.wordpress.com

Explicitly Phylogenetic ApproachesAligned environmentalsequences

Guide Tree

Evolutionary Placement of short reads

We provide:• Support for Paired End (raw) Illumina data• Marker gene data for Bacteria, Archaea,

Eukaryotes, Viruses• Taxonomy assignments based on probability

distributions over a reference phylogeny• Complement to existing tools – QIIME, etc.

– Inputs/outputs will be compatible for use with other software tools

Great!

Not Bad

Getting Tricky…

MarineMetagenome

Tree PlacementSing Tree - Guppy

AcknowledgementsUC Davis• Jonathan Eisen• Aaron Darling• Guillaume Jospin• Dongying Wu

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PhyloSift Software Development on Github:https://github.com/gjospin/PhyloSift

Google Group for user support: https://groups.google.com/d/forum/phylosift

Twitter: @PhyloSift