solaµ increasing lc-ms...

thermoscientific.com/sola-spe

- Getting more from your sampleSOLAµ increasing LC-MS sensitivity

Sample Preparation Sample Handling LC Columns and Accessories

Separation Detection Data Processing

A comprehensive product offering for your complete bioanalytical workflow Ordering informationDescription Part Number

SOLAμ HRP 96 well plate 60209-001 SOLAμ SCX 96 well plate 60209-002 SOLAμ SAX 96 well plate 60209-003 SOLAμ WCX 96 well plate 60209-004 SOLAμ WAX 96 well plate 60209-005 Complementary products Description Part Number

HyperSep-96 Well Plate Manifold 60103-351 Vacuum Pump, European Version 60104-241 Vacuum Pump, North American Version 60104-243

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No compromiseThe SOLAµ family consists of a wide range of phase chemistries suitable for almost every compound type. This allows for extremely selective analysis removing potential interferences. As a result increased concentration can be achieved without increased matrix effects.

Thermo Scientific™ SOLAμ™ is a micro elution device built on Embedded Particle Technology (EPT), which removes the need for frits and so eliminates issues associated with traditional loose-packed SPE. This results in highly reproducible, accurate data. SOLAμ enables:

• An increase in your sensitivity by up to 20 fold

• Removal of evaporation and reconstitution steps

• Removal of analyte volatility, adsorption and solubility issues

Simple workflowExchanging your traditional SPE for SOLAµ allows you to keep the workflow simple. By reducing your elution volumes you can directly inject the eluent with no further processing. This can provide up to 20-fold increase in sample concentration with no additional steps.

Make your sample work harderLow elution volumes gives you the tools to concentrate your sample pre-analysis without any need for evaporation.

This not only saves you time but avoids issues with loss of volatile compounds, or compounds susceptible to non-specific binding such as proteins and peptides.

• SOLAµ: sample enrichment 20 × preconcentration)

• Observed matrix effects on a selection of opiates extracted from human urine using SOLAµ SCX method and analysed by LC-MS/MS with electrospray ionization.

1:1 dilutionwith 4% H3PO4

SOLAμ

20 pg/mL 25 μLelution

800 pg/mL

1:1 dilutionwith 4% H3PO4

1:10 dilutionwith 4% H3PO4

Conventionalscale SPE

20 pg/mL 500 μLelution

40 pg/mL

Dry down

Re-conin 25 μL

800 pg/mL

Conventionalscale SPE

20 pg/mL

40 pg/mL500 μL sample

40 pg/mL500 μL sample

40 pg/mL500 μL sample

500 μLelution

40 pg/mL

Problem;improvement in assay

sensitivity required

Option 2; SOLAμ providestwenty fold increase insample concentration

without changes to workflow

Option 1; introduceadditional steps withassociated issues

0%-5%

-10%-15%-20%-25%-30%-35%-40%-45%-50%-55%-60%-65%-70%

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102030405060708090

100

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1.351.361.361.381.310.96 1.48 1.81 2.281.960.730.51 2.510.22 2.77

1.33 1.341.331.360.970.52 2.161.31 2.24 2.531.55 1.880.75 2.820.270.14

102030405060708090

100

Peak Area144300 counts

Peak Area7215 counts

400 pg/mL aqueoussolution of niflumic acidinjected neat

(no concentration factor)

400 pg/mL aqueoussolution of niflumic acidtaken through the extractionprocedure

(20 × concentration factor)