srinivas istp india 2011

Srinivas Garlapati

Vaccine and Infectious Disease OrganizationSaskatoon, Canada

Optimizing vaccine formulation and delivery to improve human and animal healthPartnership development activity (PDA) between Canada and India

funded by ISTP, Canada

Courtesy: Parallel solutions Inc Andrianov et al, 1998

1000X 5000X

OVA CpG Merge

Garlapati et al 2008

Garlapati et al 2010

Alum SOL

MP

MP

Single step process by simple mixing PCEP with natural poly-amine Chitosan or synthetic poly-ally-amine hydrochloride (PAH) – FDA approved

NaCl-CaCl2 PAH

Chitosan

PCEP- CaCl2 MPs

PCEP- PAH MPs

PCEP- CHIT MPs

PCEP particle uptake by DCsPCEP particle uptake by DCs

Garlapati et al manuscript in submission

Garlapati et al manuscript in submission

RSV MARKET POTENTIAL

Discovery Pre-Clinical Phase-I Phase-II Phase-III2020

MEDI-559MEDI-534 (PIV3)

Novavax VLP F & G

PEV4 (virosome)

Protollin- RSV

RSV287Nanobio RSV

Sendai Virus vectorAlpha Virus vector

Virosomes P3CSK4rRSVΔG

DNA electoporation

Adenovirus vector

0 21 44 48 50

Immunize Boost Challenge

Kill 1

Kill 2

Spleens - ELISPOTLung-Fragment culturesBAL

Groups

1.Saline/RSV challenge2.Ag IN3.Ag Triple Combo SOL IN4.Ag Triple Combo MP IN5.Ag IM6.Ag Triple Combo SOL IM7.Ag Triple Combo MP IM

Triple Combo formulation with ΔF protein

Viral SheddingLung Cytokines

RSV-specific humoral responses in serum.

Serum Virus Neutralization TitersVirus Titers

Lung Fragment Culture

Cytokine ELISPOT of Splenocytes

Cytokines in Lung Homogenates Post-RSV Challenge

Acknowledgements….Acknowledgements….

Nelson EngGael AurayTadele KirosRachelle BuchananStacy StromMonika PolewiczAleksandra GraciaLaura LatimerMarlene SniderRavinder Garg

Volker GerdtsLorne BabiukAndrew PotterSylvia v.d. HurkHeather Wilson George MutwiriJim HolmstromHugh Townsend

VIDO

Funding: - B. & M. Gates Foundation - Canadian Institute of Health Research - Krembil Foundation - National Science and Engineering Research Council - Saskatchewan Health Research Foundation

Animal CareBarry WalkerSherryOthers…