studying social behavior in zebrafish
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Studying Social Behavior in Zebrafish
An informative webinar for scientists interested in novel methods for quantitative characterization of zebrafish social interactions.
InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information regarding innovative technologies, protocols, research
tools and laboratory services.
Thank you to our event sponsor
Zebrafish Systems
The zebra fish (Danio rerio) examination systems of the MDE GmbH are supporting the methodological back-ground of the physiological, pharmacological and toxicological researches in the fields of cardiovascular function and group behavior...
Studying Social Behavior in Zebrafish
Róbert Kovács Research Associate,Szent István University
Copyright 2016 R. Kovács, MDE Ltd. and InsideScientific. All Rights Reserved.
1. Social behaviour of zebrafish
2. How to adjust and calibrate the software
3. Experiment for measuring social cohesion
4. Some tips for use
What are we going to cover today?
About shoaling in zebrafish
About shoaling in zebrafish
1. Zebrafish is a very social animal
2. Hierarchy in the aquaria
3. Is a lonely zebrafish depressed?
1. Mechanical Unit
2. PsychoFish software for measuring socialcohesion
About the PsychoFish System for Zebrafish
Lower lighting
Overhead lighting with camera
1. Monitoring and measuring groupparameters
2. More possiblilties to apply in different behaviour tests (ROI –Range of Interest, reference point)
PsychoFish & Shoaling
1. Area of the shoal
2. Longest diagonal
3. Distance of the shoalcenter from the referencepoint
4. Distance of the nearestand the farthest fish
5. Mean distance of fish and mean median distanceand coordinates
Measured Parameters
Example Data
Studies 1: Gender Experiment
1. Difference between genders for shoalssize and cohesion
2. Ethanol effects to the cohesion of shoals
• Similar size fish were used
• 2, 3, 4 and 5 adults were tested withboth gender
• Water volume and tank were thesame
• Room conditions and temperature
• Capture duration was 120 seconds
• In case of minimal distance, fish density decreased the inter-individual distance
• Maximal distance wasincreased with density
Difference between group sizeM
ale
Fem
ale
Kruskal-Wallis ANOVA, Dunn’s post-hoc, p≤0.05
A – mean area
B – minimal distance
C – maximal distance
genders
Difference between genders
• Male shoals area weresignificantly bigger with 3 and 4 fish
• Minimal and maximaldistance weresignificantly higher in 2 to4 fish groups
Kruskal-Wallis ANOVA, Dunn’s post-hoc, p≤0.05
Studies 2: Ethanol Effects
1. Difference betweengenders for shoalssize and cohesion
2. Ethanol effects tothe cohesion of shoals
• Similar-sized fish were used
• 4 adults (2 female-2 male) were tested
• Water volume and tank constant
• Room conditions and temperature
• 1 % ethanol treated fish was tested
The effects of 1% ethanol
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
5000
10000
15000
* ** *
**
*
mean
are
a (
mm
2)
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
50
100
150
200
250 * **
* * *
lon
ges d
iag
on
al
(mm
)
A B • Mean area of shoals (A) were increased afterexposure
• Longest diagonal (B) was significantly longer in EtOH treated fish
Kruskal-Wallis ANOVA, Dunn’s post-hoc, p≤0.05
The effects of 1% ethanol
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
20
40
60
80
100
* * * ** * *
min
imal
dis
tan
ce (
mm
)
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
50
100
150
200
250
**
** *
*
maxim
al
dis
tan
ce (
mm
)
A B • Minimal distance (A) wassignificantly higher afterEtOH treatment
• Mean maximal individual distance was also higher after treatment
Kruskal-Wallis ANOVA, Dunn’s post-hoc, p≤0.05
The effects of 1% ethanol
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
50
100
150 * * * * ** *
mean
dis
tan
ce (
mm
)
previ
ous
10 m
in
20 m
in
30 m
in
40 m
in
50 m
in
60 m
in
90 m
in
0
50
100
150
200
** *
* * * *
mean
med
ian
dis
tan
ce (
mm
)
A B
• Mean individual distance(A) was higher aftertreatment
• As well as mean mediandistance (B)
Kruskal-Wallis ANOVA, Dunn’s post-hoc, p≤0.05
Male and female group cohesion is different
1% EtOH cauesd significant decrease in group cohesionafter 10 minutes
Use fish from the same tank
Take care of room and water temperature
After transposition fish need 1 to 20 minutes to calm down
Tips, Pointers, Summary
Thank you!If you have questions for the presenters please contact them by email.
For additional information on the solutions presented in this webinar please visit:
http://mdegmbh.eu
Thank you for your participation
MDE Ltd.info@mdegmbh.eu
Róbert Kovácsrob.x21@gmail.com
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