szeged - timisoara axis for the safe food and feed acronym: szetisa 1

Szeged - Timisoara axis for the safe food and feed

Acronym: SZETISA 1

HURO / 0901/147/2.2.2

Szeged University

Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara

January, 26th - 27th, 2012

Determination of microelements as affected by microbial toxins in wheat

2 wheat cultivars artificially infected with 2 Fusarium strains of low and high activities, respectively, in Szeged (Cereal Research Institute).

Wheat specimen spontaneously and naturally infected - collected in Romania

The analyzed material

Artificially infected wheat cultivars – moment 0 – Fusarium strain identification

the samples were negatives

The Fusarium attack was evaluated

in different organs:

parts1 grains2 chaf3 culm4 flag leaf

GMO presence

Fusarium detection

Toxin genes identification

Corn + Soy bean

Fusarium graminearumFusarium culmorumFusarium proliferatumFusarium verticiloides

TRI 7 DONTRI 13 DONTRI 7 NIVPQF5 - fumonisin

8 samples of commercial feed samples

Wheat specimen spontaneously and naturally infected - collected in Romania

Fusarium detectionToxin genes identification

Collected from 2010 harvest

7 wheat samples, collected from three Romanian counties

WHEAT SAMPLES

18 wheat samples, collected from two Romanian counties

Fusarium detectionToxin genes identificationToxin quantification

Flag leaf

Culm

Ear Chaf

Grain Fusarium quantification

The analyzed material

DETERMINATION OF MICROELEMENTS AS AFFECTED BY MICROBIAL TOXINS IN MAIZE.

2 corn cultivars - genetically modified (Bt) and conventional from different locations

5 maize samples from last year’s harvest 4 conventional varieties YG – genetically modified maize

GMO presence

Fusarium detection

Toxin genes identification

Maize samples from this year harvestgenetically modified maize – 14 from different farms

GMO presence

Fusarium detection

Toxin genes identification

Toxin quantification

SIGMA

SATELIT

Two month treatment

The plants were grown in the green house

ALFALFA metal treatment

Untreated - control

Pb 50 Cd 1 Cu 100 Fe 100

100 3 250 250

250 10 500 500

500 20

Untreated - control

Pb 50 Cd 1 Cu 5 Fe 5100 3 10 10250 10 50 50

Dried materials

INCREASE OF INFRASTRUCTURE

COOLING CENTRIFUGE - SIGMA

Multi-Sample Micro-Volume UV-Vis Spectrophotometer

The tender was organizedThe contract was signedThe equipment was installed

The tender was organizedThe contract was signedThe equipment was installed

BIOANALYZER

The tender was organized

Ioja-Boldura Oana-Maria, Popescu Sorina

A molecular approach for screening of toxigenic Fusarium graminearum in feedstuff

Bulletin of University of Agricultural Sciences And Veterinary Medicine, Volume 68 (1-2), pag. 422, Cluj-Napoca 2011, ISSN 1843-5262

COMUNICATION ACTIVITIES

THANK YOU FOR YOUR ATTENTIONS!