test tube baby

Patel Nimesh .G Msc.Bioinformatics Ganpat University. nima_btech1989@yahoo.in

Test Tube Baby

Good Morning From Me

Defination:

Children are the gift of god , but some couple are deprived of this gift, so scientist & doctor have invented a specific technique which called Test Tube Baby.

Which also known as I.V.F & Embryo Transfer.

I.V.F is a process from there eggs cells are fertilized by sperm outside the woman’s womb ,after the embryo transfer in to woman’s uterus.

The procedure has an average success rate is only 15%.s

Procedure:

I. Before StartingII. CounselingIII. Ovarian StimulationIV. Ultrasound

ExaminationV. HCG injection.

VI. Egg CollectionVII. Sperm

CollectionVIII. After Egg

CollectionIX. Embryo TransferX. Pregnancy Test.

Before Starting:

screening test will be checked Wife: complete hemogram, blood sugar, urea Hepatitis B Oral Contraceptive pill

from the

HIV 3rd/4th/5th day of period.

Husband: Sperm test of last 6 month Hepatitis B HIV

Counseling: Couples with infertility problems have

faced many frustration and under stress . Consent must be signed before starting

treatment.

Ovarian Stimulation: Drugs are used to stimulate the ovaries to

produce several eggs instead of the usual one to increase the chance of pregnancy.

Long Protocol: start injection on the 21st day of previous cycle. Short/Antagonist Protocol: injection given started from 2nd of period. Drugs Treatment: start from 2nd of the cycle to 9-12 days daily till

the eggs become matureas determined by ultrasound exa.

Ultrasound Examination:

The examination is done after 4 days of drug to measure the response of the ovaries.

This shows the number & size of the follicles growing in the ovaries, which show when they will be mature?

Sometimes with poor chances if show then

doctor suggest cancelling the cycle.

HCG Injection

HCG is given during the final maturing of the eggs ready for collection.

Eggs collection is planned 34-36 hours after HCG injection

The embryologist will inform about the time for sperm collection by the husband.

Egg Collection: Patient admitted early in the morning. She should have nothing to eat or drink

from midnight the night before the procedure.

The eggs are collected through needle ,which is passed through the vagina into ovary, and passed immediately to the laboratory.

The procedure takes about 15 -30 minutes & patients will able to go home about 2-3 hours.

Sperm Collection:

A fresh sperm sample is collected on the day of egg collection, just before or after egg collection.

Sexual activity may continue during the treatment cycle otherwise more than 4 or 5 days without ejaculation may reduce sperm quality.

Embryo Transfer Embryo transfer is done two or three

days after egg collection. It is simple procedure from there

woman’s legs are rested in stripes and embryo passed through the cervix into the uterus through fine soft plastic tube. It takes only few minutes.

There is no need to rest afterwards; you may go home and back to work and all normal activity but heavy work like lifting weights, climbing, should be avoided.

Pregnancy Test: You will be given a date for a pregnancy

test about 14 or 15 days after embryo transfer.

It is important that you get the test done even if you have a period. As there a small chance of pregnancy even then.

If the test is negative than the further treatment will depend on whether you have frozen embryos stored or not.

First Successful “Test Tube Baby”

“Louise Joy Brown”

On July 25, 1978. Louise joy brown ,the world’s first successful “test tube baby” was born in Great Britain.

Dr.Patrick Steptoe & Dr.Edward had successfully found a way to fertilize an egg outside a woman body.

The fertilization was done under 37 C, 100%moisture,5% co2 & 95% o2.

At 11:47 p.m , on July 25,1978. a five pound 12 ounce baby girl was born ,named Louise joy brown.

India’s First

“ Test Tube Baby”

Kanupriya Agarwal(Durga)

Kanupriya (Durga) born October 3,1978.

The international scientist community is now accepting “Dr.Subhas Mukhopadhyay ‘s claim and recognizing India for producing the world’s second test tube baby.

She born just 67 days after Marie Louise Brown was born.

Poor chances of success with IVF Older woman ,whose ovaries are falling ,

however is no upper age limit at with done.

Men whose sperm count are very low.

Woman with a damaged uterus

General Information:

In Bhopal(India),on October 2009,three(3) pregnant women give birth double baby with using of “test tube baby” procedure.

Today in India, minimum 2000 hospital contain the process of test tube baby.

The procedure has an average success rate of only 15%.

The total cost having roughly around one lac ten thousand nearest.

Does not affect woman’s health.

Any Question? E-mail to Gynecologists.

Thank You